FastQCFastQC Report
Sat 18 Jun 2016
SRR3548970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790095
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78160.4366248718643424No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52880.29540331658375674No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC24580.13731114829101249No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19050.10641893307338436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT207.0343417E-445.011
ATCGTGT253.8916805E-545.017
ATATCGT207.0343417E-445.042
CGAATCG207.0343417E-445.037
ATGCGAA207.0343417E-445.040
CGCACGA207.0343417E-445.040
CACGTTA207.0343417E-445.016
TCGACGA351.2124292E-745.031
ACGGTTC207.0343417E-445.043
TACGCTA302.1661835E-644.99999632
TACTACG302.1661835E-644.9999961
CGGTCTA1150.043.0434831
ATTTACG900.042.51
ACGTTAG850.042.3529431
ACGGGTA1250.041.3999985
AGTACGG3000.041.2500042
CGCATGG1700.041.0294152
GCGAAGT556.184564E-1140.90909243
TACGGGC2150.040.8139534
AAGGGCG7250.040.344835