##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1790095
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31735075512752	31.0	31.0	34.0	30.0	34.0
2	31.56964518642865	31.0	31.0	34.0	30.0	34.0
3	31.653167569318946	31.0	31.0	34.0	30.0	34.0
4	35.41450760993131	37.0	35.0	37.0	33.0	37.0
5	35.28337993234996	37.0	35.0	37.0	33.0	37.0
6	35.2698041165413	37.0	35.0	37.0	32.0	37.0
7	35.879536560908775	37.0	35.0	37.0	35.0	37.0
8	35.97306567528539	37.0	35.0	37.0	35.0	37.0
9	37.62083967610658	39.0	37.0	39.0	35.0	39.0
10	36.90016339914921	39.0	37.0	39.0	32.0	39.0
11	36.53065619422433	39.0	35.0	39.0	32.0	39.0
12	35.517333996240424	37.0	35.0	39.0	31.0	39.0
13	35.05571324426916	37.0	34.0	39.0	30.0	39.0
14	35.825951695301086	38.0	34.0	40.0	29.0	41.0
15	36.29859420868725	38.0	35.0	40.0	31.0	41.0
16	36.2547239113008	38.0	35.0	40.0	31.0	41.0
17	36.33081484502219	37.0	35.0	40.0	31.0	41.0
18	36.4137590463076	37.0	35.0	40.0	31.0	41.0
19	36.32878701968331	37.0	35.0	40.0	31.0	41.0
20	36.1563833204383	36.0	35.0	40.0	31.0	41.0
21	36.02514782734995	36.0	34.0	40.0	31.0	41.0
22	35.903776615207576	36.0	34.0	40.0	30.0	41.0
23	35.832823956270474	36.0	34.0	40.0	30.0	41.0
24	35.65958119541142	36.0	34.0	40.0	30.0	41.0
25	35.65194640507906	36.0	34.0	40.0	30.0	41.0
26	35.497782519922126	35.0	34.0	40.0	30.0	41.0
27	35.465078110379615	36.0	34.0	40.0	30.0	41.0
28	35.50655021102232	36.0	34.0	40.0	30.0	41.0
29	35.522382890293535	36.0	34.0	40.0	30.0	41.0
30	35.447792435596995	36.0	34.0	40.0	30.0	41.0
31	35.31012152986294	36.0	34.0	40.0	29.0	41.0
32	35.14346668752217	35.0	34.0	40.0	29.0	41.0
33	34.960675271424144	35.0	34.0	40.0	28.0	41.0
34	34.79173060647619	35.0	34.0	40.0	27.0	41.0
35	34.63287646745005	35.0	33.0	40.0	27.0	41.0
36	34.402980847385194	35.0	33.0	40.0	25.0	41.0
37	34.185425913149864	35.0	33.0	40.0	24.0	41.0
38	34.248391286495966	35.0	33.0	40.0	25.0	41.0
39	34.240809565972754	35.0	33.0	40.0	25.0	41.0
40	34.019682195637664	35.0	33.0	40.0	23.0	41.0
41	34.06447199729623	35.0	33.0	40.0	24.0	41.0
42	34.00581589245264	35.0	33.0	39.0	24.0	41.0
43	33.87436309246157	35.0	33.0	39.0	23.0	41.0
44	33.83958504995545	35.0	33.0	39.0	23.0	41.0
45	33.776755423594835	35.0	33.0	39.0	23.0	41.0
46	33.71613629444247	35.0	33.0	39.0	23.0	41.0
47	33.74234998701186	35.0	33.0	39.0	23.0	41.0
48	33.7246654507163	35.0	33.0	39.0	23.0	41.0
49	33.69251017404104	35.0	33.0	39.0	24.0	41.0
50	33.45280110832107	35.0	33.0	39.0	24.0	40.0
51	33.2703392836693	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	5.0
13	5.0
14	16.0
15	62.0
16	156.0
17	404.0
18	997.0
19	1903.0
20	3234.0
21	5419.0
22	8412.0
23	12022.0
24	16701.0
25	22653.0
26	29744.0
27	34875.0
28	39712.0
29	46015.0
30	55256.0
31	67960.0
32	86128.0
33	113348.0
34	195045.0
35	287797.0
36	125563.0
37	149357.0
38	200906.0
39	286191.0
40	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.51531901938165	23.5400914476606	27.95209192808203	13.99249760487572
2	31.68474298850061	26.23011627874498	29.192584751088628	12.892555981665776
3	28.672165443733434	26.222072012937858	31.77110712001318	13.334655423315523
4	26.290727587083367	28.089067898631075	31.264206648250514	14.355997866035041
5	23.390211134045956	32.950485868068455	29.027733164999624	14.631569832885964
6	21.267139453492693	42.15072384426525	26.652216781790912	9.92991992045115
7	86.3618411313366	5.163915881559358	6.784556126909466	1.6896868601945707
8	88.33011655805977	3.9934193436661185	6.110960591476989	1.5655035067971252
9	83.9973297506557	5.415243325074926	7.611383753376217	2.976043170893165
10	55.13266055712127	21.370094883232454	13.069809144207431	10.427435415438847
11	49.32229853722847	20.289984609755347	18.127697133392363	12.260019719623818
12	43.52640502319709	21.32367276597052	21.857555045961245	13.292367164871138
13	21.143682318536168	42.3792592013273	22.276527223415517	14.20053125672101
14	14.448339333945965	44.87812099357855	26.195984012021707	14.477555660453776
15	13.610674293822395	23.217091830321852	49.434024451216274	13.738209424639475
16	15.204723771643405	17.025129951203706	48.006334859323104	19.76381141782978
17	15.130761216583476	18.229367715121263	29.754454372533303	36.88541669576196
18	19.489636024903707	23.704775444878624	36.547222354120876	20.2583661760968
19	32.326273186618586	22.451043101064467	25.95906921141057	19.263614500906375
20	34.05606964993478	22.523944259941512	25.048111971711002	18.37187411841271
21	22.473611735690007	29.439219706216708	27.423013862392775	20.664154695700507
22	21.518969663621203	25.292456545602327	23.02821917272547	30.160354618051
23	19.36701683430209	31.71407104092241	24.667405919797552	24.25150620497795
24	17.85815836589678	23.655616042723988	42.30758702750413	16.178638563875104
25	17.47834612129524	24.734888371846186	38.81229767135264	18.974467835505937
26	15.758381538409974	36.776260477795866	27.392345099003123	20.07301288479103
27	17.464212793175783	38.839949835064616	26.67998067141688	17.01585670034272
28	15.036352819263781	30.887243414455657	37.91474754133161	16.161656224948956
29	15.600959725601154	25.61741136643586	37.271150413804854	21.510478494158132
30	18.682136981556845	33.89043598244786	29.539940617676713	17.88748641831858
31	30.927911647147216	27.703445906502168	24.36976808493404	16.998874361416572
32	33.79301098545049	24.07363854990936	26.731430454808265	15.40192000983188
33	31.89043039615216	26.626631547487705	22.450707923322504	19.03223013303763
34	20.469192975791785	27.11325376586159	26.176823017772797	26.24073024057382
35	21.867945555962116	24.243629528041808	30.839704038053846	23.048720877942234
36	33.20013742287421	26.30810096670847	23.43434287007114	17.057418740346183
37	20.979501087931087	37.02635893625757	25.8121496345166	16.181990341294735
38	20.449864392671895	37.17288747245258	22.086928347378212	20.290319787497314
39	21.79074294939654	34.34851222979786	24.742038830341407	19.118705990464193
40	26.36837709730489	25.29016616436558	25.94393035006522	22.39752638826431
41	17.79642979841852	24.482890572846692	27.07174758881512	30.648932039919668
42	20.60063851359844	25.433398786097943	23.90169236828213	30.064270332021486
43	20.774092995064507	24.48411955790056	26.84818403492552	27.893603412109414
44	18.076247350000976	29.916792125557585	30.418553205276815	21.588407319164627
45	16.98233892614638	39.911904116820615	23.2624525514009	19.843304405632104
46	20.76738944022524	36.17087361285295	24.05944935883291	19.0022875880889
47	21.604607576692857	28.716129590887636	25.476078085241287	24.203184747178224
48	22.023523891190134	25.02526402230049	32.6660316910555	20.28518039545387
49	21.920065694837426	26.10565361056257	33.28387599540806	18.690404699191944
50	19.792916018423604	36.817990106670315	24.873651957019042	18.515441917887042
51	19.020107871369955	35.98836933235387	23.94219301210271	21.049329784173466
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6063.0
1	5733.5
2	5404.0
3	9933.5
4	14463.0
5	10671.5
6	6880.0
7	6986.0
8	7092.0
9	7555.5
10	8019.0
11	8161.5
12	8304.0
13	8671.0
14	9038.0
15	8469.5
16	7901.0
17	7255.0
18	6609.0
19	6814.5
20	7020.0
21	7821.0
22	8622.0
23	8154.0
24	7686.0
25	9342.5
26	12715.5
27	14432.0
28	18151.5
29	21871.0
30	25475.5
31	29080.0
32	34656.0
33	40232.0
34	43297.5
35	46363.0
36	49342.5
37	52322.0
38	67687.5
39	83053.0
40	108768.0
41	134483.0
42	165154.0
43	195825.0
44	199031.5
45	202238.0
46	181032.5
47	159827.0
48	142414.0
49	125001.0
50	112532.5
51	100064.0
52	89923.5
53	79783.0
54	71628.0
55	63473.0
56	61530.5
57	59588.0
58	58383.5
59	57179.0
60	55716.0
61	54253.0
62	49020.0
63	43787.0
64	38916.5
65	34046.0
66	29145.0
67	24244.0
68	20449.0
69	16654.0
70	15420.0
71	14186.0
72	12177.5
73	10169.0
74	8267.0
75	4935.0
76	3505.0
77	2790.0
78	2075.0
79	1644.5
80	1214.0
81	802.0
82	390.0
83	298.5
84	207.0
85	118.5
86	30.0
87	25.0
88	20.0
89	23.5
90	27.0
91	15.0
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1790095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.916985565782415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82007012698556	13.333979886321723
2	6.951535638292605	2.351980561060361
3	2.4202135929565	1.228281552544665
4	1.3081204420164743	0.8851781854359043
5	0.8255396793034087	0.6982821419378203
6	0.5751218123979874	0.5837596439342025
7	0.4449858212537839	0.5269473100589667
8	0.37602358354639265	0.5088948428198482
9	0.3363975951271728	0.5121749935017269
>10	5.020285050136675	21.34285694602898
>50	1.6565536040831637	19.379360212665016
>100	1.2505053475680958	36.15825173969185
>500	0.011922551665726346	1.196734773240132
>1k	0.002043865999838802	0.5381762434289785
>5k	6.812886666129341E-4	0.7551409673298355
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7816	0.4366248718643424	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5288	0.29540331658375674	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2458	0.13731114829101249	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1905	0.10641893307338436	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016535435270195156	0.0
2	0.0	0.0	0.0	0.05720366796175622	0.0
3	0.0	0.0	0.0	0.08334753183490262	0.0
4	0.0	0.0	0.0	0.11396043226756122	0.0
5	0.0	0.0	0.0	0.20825710367326875	0.0
6	0.0	0.0	0.0	0.2955150424977445	0.0
7	0.0	0.0	0.0	0.3602602096536776	0.0
8	5.586295699390256E-5	0.0	0.0	0.5272345881084524	0.0
9	5.586295699390256E-5	0.0	0.0	0.5958901622539586	0.0
10	5.586295699390256E-5	0.0	0.0	0.7405193579111723	0.0
11	5.586295699390256E-5	0.0	0.0	0.8500107536192213	0.0
12	5.586295699390256E-5	0.0	0.0	0.9636360081448191	0.0
13	5.586295699390256E-5	0.0	0.0	1.0077677441700021	0.0
14	5.586295699390256E-5	0.0	0.0	1.0251411237951058	0.0
15	5.586295699390256E-5	0.0	0.0	1.049218058259478	0.0
16	5.586295699390256E-5	0.0	0.0	1.0964781198763194	0.0
17	5.586295699390256E-5	0.0	0.0	1.1497155178915086	0.0
18	5.586295699390256E-5	0.0	0.0	1.243565285641265	0.0
19	5.586295699390256E-5	0.0	0.0	1.2878646105374296	0.0
20	1.1172591398780512E-4	0.0	0.0	1.3319404836056188	0.0
21	1.1172591398780512E-4	0.0	0.0	1.391322806890137	0.0
22	1.1172591398780512E-4	0.0	0.0	1.4599783810356433	0.0
23	1.1172591398780512E-4	0.0	0.0	1.5360078655043448	0.0
24	2.2345182797561025E-4	0.0	0.0	1.6002502660473328	0.0
25	2.2345182797561025E-4	0.0	0.0	1.6469516980942351	0.0
26	2.2345182797561025E-4	0.0	0.0	1.6943793485820584	0.0
27	2.2345182797561025E-4	0.0	0.0	1.7432594359517233	0.0
28	2.2345182797561025E-4	0.0	0.0	1.795100260042065	0.0
29	2.2345182797561025E-4	0.0	0.0	1.8519128873048638	0.0
30	2.2345182797561025E-4	0.0	0.0	1.937662526290504	0.0
31	2.2345182797561025E-4	0.0	0.0	2.0108988629095106	0.0
32	2.2345182797561025E-4	0.0	0.0	2.076426111463358	0.0
33	2.2345182797561025E-4	0.0	0.0	2.1455844522218093	0.0
34	2.2345182797561025E-4	0.0	0.0	2.2189325147548034	0.0
35	2.2345182797561025E-4	0.0	0.0	2.3230610665914377	0.0
36	2.2345182797561025E-4	0.0	0.0	2.4057382429424137	0.0
37	2.2345182797561025E-4	0.0	0.0	2.4913761560140664	0.0
38	2.793147849695128E-4	0.0	0.0	2.5738298805370663	0.0
39	2.793147849695128E-4	0.0	0.0	2.6548870311352193	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.0343417E-4	45.0	11
ATCGTGT	25	3.8916805E-5	45.0	17
ATATCGT	20	7.0343417E-4	45.0	42
CGAATCG	20	7.0343417E-4	45.0	37
ATGCGAA	20	7.0343417E-4	45.0	40
CGCACGA	20	7.0343417E-4	45.0	40
CACGTTA	20	7.0343417E-4	45.0	16
TCGACGA	35	1.2124292E-7	45.0	31
ACGGTTC	20	7.0343417E-4	45.0	43
TACGCTA	30	2.1661835E-6	44.999996	32
TACTACG	30	2.1661835E-6	44.999996	1
CGGTCTA	115	0.0	43.04348	31
ATTTACG	90	0.0	42.5	1
ACGTTAG	85	0.0	42.352943	1
ACGGGTA	125	0.0	41.399998	5
AGTACGG	300	0.0	41.250004	2
CGCATGG	170	0.0	41.029415	2
GCGAAGT	55	6.184564E-11	40.909092	43
TACGGGC	215	0.0	40.813953	4
AAGGGCG	725	0.0	40.34483	5
>>END_MODULE