FastQCFastQC Report
Sat 18 Jun 2016
SRR3548969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1784754
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71170.3987664406411191No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46700.26166071066376656No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT27820.15587582378299755No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25640.14366125527663756No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC18700.10477634452703286No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAT207.034335E-445.00000423
AATCGGC207.034335E-445.00000420
TCTATCG207.034335E-445.0000041
GTCGAAT253.8916754E-545.018
CTATCGA253.8916754E-545.026
AACGATC253.8916754E-545.024
TAGTACG253.8916754E-545.01
GGACGTA600.044.9999969
TAATGCG800.042.1875041
TCGATTG603.6379788E-1241.2499961
TACGATG603.6379788E-1241.2499961
TCGAACG556.184564E-1140.9090921
ACGTAGG2500.040.4999962
CGGGATA1300.039.807696
ACGGGTA2500.039.65
CGAAAGG2050.039.5121962
TCTACGG403.4596633E-739.3750042
AGGCACG403.4596633E-739.3750041
TTACGCG403.4596633E-739.3750042
GACCGAT7000.039.2142879
Produced by FastQC (version 0.11.5)