##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548969_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1784754 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.14410725511751 31.0 31.0 33.0 30.0 34.0 2 31.406336671608525 31.0 31.0 34.0 30.0 34.0 3 31.515325921667635 31.0 31.0 34.0 30.0 34.0 4 35.28108915850588 37.0 35.0 37.0 33.0 37.0 5 35.12181006458033 37.0 35.0 37.0 32.0 37.0 6 35.11818323421603 37.0 35.0 37.0 32.0 37.0 7 35.79359676459613 37.0 35.0 37.0 35.0 37.0 8 35.883322295397576 37.0 35.0 37.0 35.0 37.0 9 37.50775625100154 39.0 37.0 39.0 35.0 39.0 10 36.70238699563077 39.0 35.0 39.0 32.0 39.0 11 36.33661725929736 38.0 35.0 39.0 32.0 39.0 12 35.4097713186243 37.0 35.0 39.0 30.0 39.0 13 34.95945099436673 37.0 34.0 39.0 29.0 39.0 14 35.7417952278017 38.0 34.0 40.0 27.0 41.0 15 36.193561129432965 38.0 35.0 40.0 30.0 41.0 16 36.132022676514524 38.0 34.0 40.0 31.0 41.0 17 36.196076882304226 37.0 34.0 40.0 31.0 41.0 18 36.292251481156505 37.0 35.0 40.0 31.0 41.0 19 36.21410065476811 37.0 35.0 40.0 31.0 41.0 20 36.082950927690874 37.0 34.0 40.0 31.0 41.0 21 35.94257752048742 37.0 34.0 40.0 30.0 41.0 22 35.815937658635306 37.0 34.0 40.0 30.0 41.0 23 35.75638099144196 36.0 34.0 40.0 30.0 41.0 24 35.54949589691353 36.0 34.0 40.0 29.0 41.0 25 35.537125564643645 36.0 34.0 40.0 30.0 41.0 26 35.39863869194298 36.0 34.0 40.0 29.0 41.0 27 35.34156079773459 36.0 34.0 40.0 29.0 41.0 28 35.37547695648812 36.0 34.0 40.0 29.0 41.0 29 35.389814506649095 36.0 34.0 40.0 29.0 41.0 30 35.29526478158895 36.0 34.0 40.0 29.0 41.0 31 35.17631897729323 36.0 34.0 40.0 29.0 41.0 32 35.03750881073806 36.0 34.0 40.0 28.0 41.0 33 34.85729742026072 36.0 34.0 40.0 27.0 41.0 34 34.70927814141333 35.0 34.0 40.0 26.0 41.0 35 34.56337175879701 35.0 33.0 40.0 26.0 41.0 36 34.32991605565809 35.0 33.0 40.0 24.0 41.0 37 34.15016971526608 35.0 33.0 40.0 24.0 41.0 38 34.22232083525237 35.0 33.0 40.0 24.0 41.0 39 34.21511592073754 35.0 33.0 40.0 24.0 41.0 40 34.01326625406078 35.0 33.0 40.0 23.0 41.0 41 34.02595315656948 35.0 33.0 40.0 23.0 41.0 42 33.99436168794131 35.0 33.0 40.0 24.0 41.0 43 33.87017650611793 35.0 33.0 40.0 23.0 41.0 44 33.82513892670923 35.0 33.0 39.0 23.0 41.0 45 33.78857758548237 35.0 33.0 39.0 23.0 41.0 46 33.70140758894503 35.0 33.0 39.0 23.0 41.0 47 33.72286152601423 35.0 33.0 39.0 23.0 41.0 48 33.698815074794624 35.0 33.0 39.0 23.0 41.0 49 33.69130087395798 35.0 33.0 39.0 24.0 41.0 50 33.46469541460616 35.0 32.0 39.0 24.0 41.0 51 33.28469525772179 35.0 32.0 39.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 4.0 13 6.0 14 13.0 15 67.0 16 192.0 17 558.0 18 1226.0 19 2347.0 20 3961.0 21 6122.0 22 9115.0 23 12807.0 24 17714.0 25 23809.0 26 30697.0 27 37125.0 28 42119.0 29 48512.0 30 58571.0 31 70985.0 32 87890.0 33 113548.0 34 187318.0 35 262728.0 36 127084.0 37 152995.0 38 203812.0 39 283234.0 40 193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.967232458927114 24.4542945414326 28.46907753113314 14.109395468507143 2 30.207076157274336 26.799939935699825 29.859801406804525 13.133182500221318 3 28.889135421464246 26.128754999288418 31.697981906750172 13.284127672497162 4 26.78951833137788 28.33751878410134 30.090813635940865 14.782149248579914 5 23.54727878463923 33.46836594847245 28.194641950655384 14.78971331623294 6 21.198383642787746 42.040079473137475 26.583439510431127 10.17809737364365 7 86.92060642531128 5.154659969945438 6.215310345291284 1.7094232594520027 8 88.37923881946756 4.001952089755787 5.8933612139264016 1.725447876850255 9 84.01197027713623 5.676804758526945 7.390374247655419 2.9208507166814024 10 52.60114279054704 23.444799675473483 13.309397261471329 10.644660272508144 11 45.792249239951275 21.052257061757533 20.178803353291265 12.976690344999927 12 42.30420550955482 21.03752113736683 23.306181131965527 13.352092221112827 13 21.005864113485668 41.550095979614 22.404208086940834 15.0398318199595 14 15.032772023483348 42.399400701721355 26.627367132949413 15.94046014184588 15 14.307854191670113 24.03552534410905 46.68822706098432 14.968393403236524 16 16.069497532993342 18.499356213797533 45.49876341501406 19.932382838195068 17 15.462186945651894 19.60298169943869 29.641451987220645 35.29337936768877 18 20.07918178079444 23.80294427131134 35.60776443140063 20.510109516493593 19 30.65268378723342 24.12410898084554 25.25916736984481 19.96403986207623 20 32.10291166177524 23.130806822676963 25.804788783216065 18.961492732331738 21 21.571768434193174 29.969788553492528 27.444678650391037 21.01376436192327 22 20.972974426727717 26.01406132161631 23.300746209281503 29.712218042374467 23 19.321934563530885 31.679940204644453 25.221907332887334 23.776217898937333 24 18.119640017615872 23.84474274886063 41.10319965664736 16.93241757687614 25 17.079272549606276 26.299534837854406 36.12044012788317 20.500752484656147 26 16.622626983886853 35.53049888107829 26.426499114163633 21.420375020871223 27 16.545417463695276 36.977757158689656 27.636581848254714 18.840243529360347 28 14.694294003543346 31.016879637193696 36.85393056970317 17.434895789559793 29 15.866164188453983 25.66297652225461 36.163583328570766 22.307275960720638 30 18.621782049514948 33.4922908143083 29.3973847376165 18.488542398560252 31 29.64565424702788 27.82775665441848 24.50438547833483 18.02220362021881 32 31.86539993747037 25.30320705262462 25.715308664387358 17.116084345517645 33 29.698042419291397 27.081603403046024 22.806280305296976 20.414073872365606 34 19.350453900089313 26.718472125570248 27.272273938032914 26.65880003630752 35 21.176139680874787 24.556773650598345 31.320843096583616 22.94624357194325 36 30.46352606577713 27.7469051757273 23.232165329227445 18.557403429268124 37 20.220825951363604 37.36105928324015 25.69877977581224 16.719334989584 38 19.979448148036088 35.82023068725438 23.695982751684546 20.50433841302499 39 21.471026259081082 34.83370817490814 24.441575701749375 19.253689864261407 40 25.856056352864314 25.414538922450937 26.07989672526298 22.64950799942177 41 16.638819691677394 25.587279815593632 27.08927953096057 30.6846209617684 42 19.43679633159528 25.8779641339927 24.062980108182977 30.62225942622905 43 20.105459912122342 25.89258799812187 26.613639750912448 27.388312338843335 44 17.609541707148434 29.987606135075197 30.379648960024742 22.023203197751624 45 16.589905387521192 39.82498428354832 22.395803567326368 21.189306761604122 46 19.278959453235572 36.18605141100678 24.866003942280003 19.668985193477646 47 20.382304788222918 28.510819978551666 26.94819566169904 24.158679571526385 48 20.3788309201156 24.986972994597576 33.38936346409645 21.244832621190373 49 20.403540207782136 27.047368993149757 32.58275370163059 19.966337097437517 50 18.471957479854368 35.92371833877386 26.48449029950346 19.119833881868313 51 17.62242863722395 35.2860394205588 25.061324978120236 22.03020696409701 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5288.0 1 4948.5 2 4609.0 3 8868.5 4 13128.0 5 9798.0 6 6468.0 7 6513.0 8 6558.0 9 6904.0 10 7250.0 11 7751.5 12 8253.0 13 8156.5 14 8060.0 15 7836.0 16 7612.0 17 7252.5 18 6893.0 19 7010.5 20 7128.0 21 7465.5 22 7803.0 23 8517.5 24 9232.0 25 10349.5 26 13750.0 27 16033.0 28 20530.5 29 25028.0 30 29986.5 31 34945.0 32 38710.0 33 42475.0 34 48473.5 35 54472.0 36 56065.5 37 57659.0 38 70685.0 39 83711.0 40 111295.5 41 138880.0 42 161134.5 43 183389.0 44 184617.5 45 185846.0 46 168487.0 47 151128.0 48 137683.5 49 124239.0 50 115660.5 51 107082.0 52 99066.0 53 91050.0 54 79232.0 55 67414.0 56 62812.0 57 58210.0 58 57884.0 59 57558.0 60 55001.5 61 52445.0 62 47219.0 63 41993.0 64 36354.0 65 30715.0 66 26773.0 67 22831.0 68 19384.0 69 15937.0 70 13803.0 71 11669.0 72 10099.5 73 8530.0 74 6976.0 75 4264.5 76 3107.0 77 2347.5 78 1588.0 79 1291.5 80 995.0 81 711.5 82 428.0 83 285.5 84 143.0 85 93.5 86 44.0 87 33.0 88 22.0 89 16.0 90 10.0 91 7.0 92 4.0 93 3.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1784754.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.615010778301208 #Duplication Level Percentage of deduplicated Percentage of total 1 77.74798053360045 14.47279495624125 2 6.909574425706154 2.572436048159889 3 2.7359638109015254 1.5278998748692185 4 1.5828680906665213 1.1786042627354538 5 0.9860812255711847 0.9177956321144034 6 0.7488975198695091 0.8364441242528373 7 0.5746485719459538 0.7487962552356529 8 0.45595025606375555 0.6790015144796805 9 0.39673243467129765 0.6646660692757098 >10 5.391658729149435 24.639799856992802 >50 1.4089626450441064 18.11352367363248 >100 1.0507398071270844 31.50495941348962 >500 0.008077834117439574 0.9745644228230306 >1k 0.0015534296379691491 0.757107759727986 >5k 3.106859275938298E-4 0.4116061359700616 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7117 0.3987664406411191 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4670 0.26166071066376656 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 2782 0.15587582378299755 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 2564 0.14366125527663756 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 1870 0.10477634452703286 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.6030130763119175E-5 0.0 0.0 0.0178175815826719 0.0 2 5.6030130763119175E-5 0.0 0.0 0.055133648670909266 0.0 3 5.6030130763119175E-5 0.0 0.0 0.07984293633744483 0.0 4 5.6030130763119175E-5 0.0 0.0 0.11497382832592054 0.0 5 5.6030130763119175E-5 0.0 0.0 0.2103931410155125 0.0 6 5.6030130763119175E-5 0.0 0.0 0.3235179750262501 0.0 7 5.6030130763119175E-5 0.0 0.0 0.405265935809641 0.0 8 5.6030130763119175E-5 0.0 0.0 0.6272573138931191 0.0 9 5.6030130763119175E-5 0.0 0.0 0.7236851689364473 0.0 10 5.6030130763119175E-5 0.0 0.0 0.8676265748669004 0.0 11 5.6030130763119175E-5 0.0 0.0 0.9845614577695302 0.0 12 5.6030130763119175E-5 0.0 0.0 1.0995352860954506 0.0 13 1.1206026152623835E-4 0.0 0.0 1.148113409467075 0.0 14 1.1206026152623835E-4 0.0 0.0 1.1677799853649298 0.0 15 1.1206026152623835E-4 0.0 0.0 1.193721935908254 0.0 16 1.1206026152623835E-4 0.0 0.0 1.249640006409847 0.0 17 1.1206026152623835E-4 0.0 0.0 1.3157555607103275 0.0 18 1.6809039228935752E-4 0.0 0.0 1.4201957244527816 0.0 19 1.6809039228935752E-4 0.0 0.0 1.4684376670398274 0.0 20 2.241205230524767E-4 0.0 0.0 1.522338652833948 5.6030130763119175E-5 21 2.8015065381559585E-4 0.0 0.0 1.5934408887723461 5.6030130763119175E-5 22 2.8015065381559585E-4 0.0 0.0 1.6764775425632887 5.6030130763119175E-5 23 2.8015065381559585E-4 0.0 0.0 1.7764352958446934 5.6030130763119175E-5 24 3.922109153418342E-4 0.0 0.0 1.8508433094981156 5.6030130763119175E-5 25 3.922109153418342E-4 0.0 0.0 1.9165106227524913 5.6030130763119175E-5 26 4.482410461049534E-4 0.0 0.0 1.9773033146304757 5.6030130763119175E-5 27 4.482410461049534E-4 0.0 0.0 2.039328669385249 5.6030130763119175E-5 28 4.482410461049534E-4 0.0 0.0 2.104715831985809 5.6030130763119175E-5 29 4.482410461049534E-4 0.0 0.0 2.180020327731441 5.6030130763119175E-5 30 4.482410461049534E-4 0.0 0.0 2.2851328530430526 5.6030130763119175E-5 31 4.482410461049534E-4 0.0 0.0 2.378030809848304 5.6030130763119175E-5 32 4.482410461049534E-4 0.0 0.0 2.4676229889385315 5.6030130763119175E-5 33 4.482410461049534E-4 0.0 0.0 2.554413661490603 5.6030130763119175E-5 34 4.482410461049534E-4 0.0 0.0 2.645518654111435 5.6030130763119175E-5 35 4.482410461049534E-4 0.0 0.0 2.7713062976746374 5.6030130763119175E-5 36 4.482410461049534E-4 0.0 0.0 2.8720484727867257 5.6030130763119175E-5 37 4.482410461049534E-4 0.0 0.0 2.9730707985526297 5.6030130763119175E-5 38 4.482410461049534E-4 0.0 0.0 3.072356190264877 5.6030130763119175E-5 39 4.482410461049534E-4 0.0 0.0 3.1948380561130554 5.6030130763119175E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGATAT 20 7.034335E-4 45.000004 23 AATCGGC 20 7.034335E-4 45.000004 20 TCTATCG 20 7.034335E-4 45.000004 1 GTCGAAT 25 3.8916754E-5 45.0 18 CTATCGA 25 3.8916754E-5 45.0 26 AACGATC 25 3.8916754E-5 45.0 24 TAGTACG 25 3.8916754E-5 45.0 1 GGACGTA 60 0.0 44.999996 9 TAATGCG 80 0.0 42.187504 1 TCGATTG 60 3.6379788E-12 41.249996 1 TACGATG 60 3.6379788E-12 41.249996 1 TCGAACG 55 6.184564E-11 40.909092 1 ACGTAGG 250 0.0 40.499996 2 CGGGATA 130 0.0 39.80769 6 ACGGGTA 250 0.0 39.6 5 CGAAAGG 205 0.0 39.512196 2 TCTACGG 40 3.4596633E-7 39.375004 2 AGGCACG 40 3.4596633E-7 39.375004 1 TTACGCG 40 3.4596633E-7 39.375004 2 GACCGAT 700 0.0 39.214287 9 >>END_MODULE