##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548967_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1586616
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.195786504106852	31.0	31.0	33.0	30.0	34.0
2	31.456058050593214	31.0	31.0	34.0	30.0	34.0
3	31.5531016956844	31.0	31.0	34.0	30.0	34.0
4	35.32540766007654	37.0	35.0	37.0	33.0	37.0
5	35.17764663913638	37.0	35.0	37.0	32.0	37.0
6	35.16773560836396	37.0	35.0	37.0	32.0	37.0
7	35.80539399577466	37.0	35.0	37.0	35.0	37.0
8	35.885410206376335	37.0	35.0	37.0	35.0	37.0
9	37.4901935944173	39.0	37.0	39.0	35.0	39.0
10	36.744151073731764	39.0	37.0	39.0	32.0	39.0
11	36.43319681636893	38.0	35.0	39.0	32.0	39.0
12	35.65831177802316	37.0	35.0	39.0	31.0	39.0
13	35.3301744089307	37.0	35.0	39.0	30.0	39.0
14	36.14078957983532	38.0	35.0	40.0	30.0	41.0
15	36.498487976926995	38.0	35.0	40.0	31.0	41.0
16	36.373460244949	38.0	35.0	40.0	31.0	41.0
17	36.425861708189004	38.0	35.0	40.0	31.0	41.0
18	36.496864395669775	38.0	35.0	40.0	31.0	41.0
19	36.40580518537567	37.0	35.0	40.0	31.0	41.0
20	36.276603160437055	37.0	35.0	40.0	31.0	41.0
21	36.174371114371716	37.0	34.0	40.0	30.0	41.0
22	36.05222372647194	37.0	34.0	40.0	30.0	41.0
23	35.953267835443484	37.0	34.0	40.0	30.0	41.0
24	35.757442254458546	37.0	34.0	40.0	30.0	41.0
25	35.71540687853898	37.0	34.0	40.0	30.0	41.0
26	35.55827623066955	36.0	34.0	40.0	30.0	41.0
27	35.55117810484705	37.0	34.0	40.0	29.0	41.0
28	35.53978782515744	36.0	34.0	40.0	29.0	41.0
29	35.50424488345006	36.0	34.0	40.0	29.0	41.0
30	35.40196178533432	36.0	34.0	40.0	29.0	41.0
31	35.28646187861461	36.0	34.0	40.0	29.0	41.0
32	35.148192757415785	36.0	34.0	40.0	28.0	41.0
33	34.97362814947032	36.0	34.0	40.0	27.0	41.0
34	34.80874452293435	36.0	34.0	40.0	26.0	41.0
35	34.63025079792464	36.0	33.0	40.0	26.0	41.0
36	34.42584406056664	36.0	33.0	40.0	24.0	41.0
37	34.25511465912357	35.0	33.0	40.0	24.0	41.0
38	34.277610965728314	35.0	33.0	40.0	24.0	41.0
39	34.27057397631185	35.0	33.0	40.0	24.0	41.0
40	34.0931599076273	35.0	33.0	40.0	23.0	41.0
41	34.077236710079816	35.0	33.0	40.0	23.0	41.0
42	33.99555784134283	35.0	33.0	40.0	23.0	41.0
43	33.86958533129629	35.0	33.0	40.0	23.0	41.0
44	33.799722176002255	35.0	33.0	39.0	23.0	41.0
45	33.72792786660414	35.0	33.0	39.0	23.0	41.0
46	33.63730921659683	35.0	33.0	39.0	23.0	41.0
47	33.635427223726474	35.0	33.0	39.0	23.0	41.0
48	33.56425625356104	35.0	33.0	39.0	23.0	41.0
49	33.52717103571374	35.0	32.0	39.0	23.0	41.0
50	33.341867219289355	35.0	32.0	39.0	23.0	41.0
51	33.1844390829287	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	4.0
13	7.0
14	7.0
15	43.0
16	158.0
17	427.0
18	943.0
19	1870.0
20	3195.0
21	5196.0
22	7563.0
23	10907.0
24	15301.0
25	20762.0
26	27171.0
27	32634.0
28	37071.0
29	42814.0
30	51033.0
31	61653.0
32	76530.0
33	99070.0
34	158409.0
35	219601.0
36	121834.0
37	145399.0
38	190878.0
39	255936.0
40	194.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.4591255855229	23.731703197244954	27.639643114654085	14.169528102578067
2	31.838516692129666	26.319474907602093	28.766506829629858	13.075501570638389
3	30.321010250747506	25.80908045803143	30.56467349377543	13.305235797445633
4	27.48604577289023	27.791034503622804	29.820952265702605	14.901967457784366
5	23.349001900901037	32.53931638153151	29.36803864325079	14.743643074316658
6	22.064318020239302	42.09714259783085	25.949126946910912	9.889412435018933
7	85.5186762266358	5.48544827481886	7.086654867970574	1.9092206305747577
8	86.5100314127678	4.731579663888427	6.893224321448921	1.8651646018948504
9	81.8613325467536	6.538507111991812	8.38867123487977	3.2114891063748257
10	49.61603815920172	23.951353068417312	14.383820659819389	12.048788112561578
11	43.45254302238223	22.067469381375204	20.145202115697813	14.334785480544756
12	38.764830305505555	21.722899554775697	24.805434963469423	14.706835176249324
13	22.106609286683103	37.38150882129009	24.505551437777005	16.006330454249802
14	16.623366964659375	39.445587338083065	27.61878110393441	16.312264593323146
15	15.991140893574753	23.7461994584701	44.02073343518533	16.24192621276982
16	17.639680930987712	19.374064045742635	42.301918044441756	20.684336978827893
17	17.00285387264467	20.123268642191935	30.175606447936993	32.6982710372264
18	21.40158677335915	23.73668234783968	34.65268218648999	20.209048692311182
19	30.872498449530323	23.037269257337627	25.869082374059005	20.221149919073046
20	31.33209295759024	22.71854059205252	25.90065901264074	20.048707437716498
21	23.59001800057481	28.586311999878987	27.36547469583062	20.45819530371558
22	23.36501081547142	24.654232656168855	24.415485536512932	27.565270991846795
23	20.88350300261689	30.30979140510369	25.595733309130882	23.210972283148536
24	19.73376040579447	24.306259359542572	38.33870325270891	17.621276981954047
25	19.920131903371704	25.498167168363363	34.601125918306636	19.9805750099583
26	17.353348258179675	33.76109909392064	27.22334830860145	21.66220433929823
27	19.48373141327202	33.93328946638633	27.219692729683803	19.36328639065785
28	17.28508977597604	29.49718142260005	35.38146596277864	17.83626283864527
29	17.589133098367846	26.654086433012147	34.15325447367227	21.603525994947738
30	20.019840969711638	31.61811049428469	29.59657535282639	18.765473183177278
31	29.73731514115577	27.05519167838973	24.509711234476395	18.697781945978107
32	30.7693228859409	24.778333257700666	26.764636181659583	17.687707674698856
33	29.185826942372948	26.00824648182043	23.980093481976734	20.82583309382989
34	21.085631305873633	26.457000307572848	27.14229530018606	25.315073086367462
35	22.481495207409985	24.789552103344477	30.280420719317085	22.44853196992845
36	30.448010104524347	27.67298451547192	23.852337301527278	18.026668078476455
37	21.49234597407312	34.40693904511237	26.14079273119646	17.959922249618053
38	20.983779313961286	35.586241409389544	23.22219112879235	20.207788147856824
39	22.71242695144887	32.040329859272816	24.43830139113686	20.808941798141454
40	25.17420724359265	25.43034987671875	26.618097888840147	22.777344990848448
41	18.5509915442678	25.12107529484135	27.589851608706834	28.73808155218402
42	20.298799457461666	26.66562041476955	24.5506159020204	28.48496422574838
43	20.810013260927658	25.514050028488306	26.78896468962875	26.88697202095529
44	19.13481270830497	28.867413413201433	30.121340009176762	21.876433869316834
45	18.447815980678374	36.530137096814855	23.943852828913865	21.078194093592906
46	21.235194905383533	34.27975010966737	24.76144196201223	19.723613022936867
47	21.904670065094518	27.922509290212627	26.35993838458707	23.812882260105788
48	21.596466946003318	26.515489570255184	31.663237985750804	20.22480549799069
49	22.268148058509432	26.83554180721737	31.106266418591517	19.790043715681676
50	19.938157689069065	33.791919405829766	26.283801499543685	19.98612140555749
51	19.39669081869841	33.007482591881086	25.494196453332123	22.101630136088378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3974.0
1	4014.0
2	4054.0
3	9200.0
4	14346.0
5	10562.5
6	6779.0
7	6705.5
8	6632.0
9	6999.5
10	7367.0
11	7798.0
12	8229.0
13	8310.0
14	8391.0
15	8000.0
16	7609.0
17	7237.5
18	6866.0
19	6623.5
20	6381.0
21	6440.0
22	6499.0
23	7058.0
24	7617.0
25	8599.0
26	12783.0
27	15985.0
28	18688.5
29	21392.0
30	27355.5
31	33319.0
32	34442.5
33	35566.0
34	40080.0
35	44594.0
36	44549.5
37	44505.0
38	51605.0
39	58705.0
40	82079.5
41	105454.0
42	126632.5
43	147811.0
44	149514.5
45	151218.0
46	140804.5
47	130391.0
48	122352.5
49	114314.0
50	104293.5
51	94273.0
52	86981.5
53	79690.0
54	72461.0
55	65232.0
56	64395.0
57	63558.0
58	60656.5
59	57755.0
60	55477.0
61	53199.0
62	47754.5
63	42310.0
64	38327.0
65	34344.0
66	29308.0
67	24272.0
68	21825.0
69	19378.0
70	17905.5
71	16433.0
72	14211.5
73	11990.0
74	9700.0
75	6043.0
76	4676.0
77	3596.5
78	2517.0
79	1810.5
80	1104.0
81	817.5
82	531.0
83	390.0
84	249.0
85	162.5
86	76.0
87	55.0
88	34.0
89	19.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1586616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.132455213194746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.94194131690573	15.490301875788628
2	6.7747140825001555	2.7278325569626825
3	2.658786781178407	1.6058371738052548
4	1.5034672321845506	1.2107394686584532
5	1.0168603715100664	1.0235947943749493
6	0.7694179820856829	0.9294163838740013
7	0.6284485445451203	0.885654852379644
8	0.539044973927153	0.8681839036388911
9	0.49873109001286503	0.9036613199800625
>10	6.709593242905811	32.189832010289514
>50	1.1826800904793604	16.63363197547517
>100	0.7669688727277272	22.9926371370222
>500	0.005478349090895037	0.7706210077735843
>1k	0.0035448141176379643	1.26349925293741
>5k	3.222558288761786E-4	0.5045562870394987
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7777	0.4901627110781689	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3414	0.21517493835937618	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2962	0.18668663369082375	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2211	0.13935318942957842	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1966	0.1239115198636595	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1672	0.10538151638455683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019349357374437166	0.0
2	0.0	0.0	0.0	0.06403565828152495	0.0
3	0.0	0.0	0.0	0.0937214801817201	0.0
4	0.0	0.0	0.0	0.1307814871399255	0.0
5	0.0	0.0	0.0	0.2389362013240759	0.0
6	0.0	0.0	0.0	0.36291074841045345	0.0
7	0.0	0.0	0.0	0.44245110348061534	0.0
8	0.0	0.0	0.0	0.6440121617328957	0.0
9	0.0	0.0	0.0	0.7367882335738453	0.0
10	6.302722271803637E-5	0.0	0.0	0.8983270054001724	0.0
11	6.302722271803637E-5	0.0	0.0	1.0407685287429347	0.0
12	6.302722271803637E-5	0.0	0.0	1.1686507636378305	0.0
13	6.302722271803637E-5	0.0	0.0	1.2239256379615484	0.0
14	6.302722271803637E-5	0.0	0.0	1.2478759825944021	0.0
15	6.302722271803637E-5	0.0	0.0	1.2849359895526076	0.0
16	6.302722271803637E-5	0.0	0.0	1.3569130778966052	6.302722271803637E-5
17	6.302722271803637E-5	0.0	0.0	1.441999828565954	6.302722271803637E-5
18	6.302722271803637E-5	0.0	0.0	1.5754914862827551	6.302722271803637E-5
19	6.302722271803637E-5	0.0	0.0	1.6279931628068796	6.302722271803637E-5
20	6.302722271803637E-5	0.0	0.0	1.689318650511529	6.302722271803637E-5
21	6.302722271803637E-5	0.0	0.0	1.7688590055816908	6.302722271803637E-5
22	6.302722271803637E-5	0.0	0.0	1.8535045656920137	6.302722271803637E-5
23	6.302722271803637E-5	0.0	0.0	1.954789312599898	6.302722271803637E-5
24	6.302722271803637E-5	0.0	0.0	2.038237355478578	6.302722271803637E-5
25	6.302722271803637E-5	0.0	0.0	2.10013008818769	6.302722271803637E-5
26	6.302722271803637E-5	0.0	0.0	2.16593050870532	6.302722271803637E-5
27	1.2605444543607275E-4	0.0	0.0	2.23463018146798	6.302722271803637E-5
28	1.2605444543607275E-4	0.0	0.0	2.3090023042752628	6.302722271803637E-5
29	1.2605444543607275E-4	0.0	0.0	2.392387319931225	6.302722271803637E-5
30	1.2605444543607275E-4	0.0	0.0	2.505395130264664	6.302722271803637E-5
31	1.2605444543607275E-4	0.0	0.0	2.6000620187871544	6.302722271803637E-5
32	1.2605444543607275E-4	0.0	0.0	2.6991408128999077	6.302722271803637E-5
33	1.2605444543607275E-4	0.0	0.0	2.7916017486272673	6.302722271803637E-5
34	1.8908166815410912E-4	0.0	0.0	2.892319250530689	6.302722271803637E-5
35	1.8908166815410912E-4	0.0	0.0	3.018814886525788	6.302722271803637E-5
36	1.8908166815410912E-4	0.0	0.0	3.124448511801217	6.302722271803637E-5
37	1.8908166815410912E-4	0.0	0.0	3.226615639827154	6.302722271803637E-5
38	1.8908166815410912E-4	6.302722271803637E-5	0.0	3.335148517347613	6.302722271803637E-5
39	1.8908166815410912E-4	6.302722271803637E-5	0.0	3.4577364655341936	6.302722271803637E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGTA	20	7.0341E-4	45.0	21
CGTATTA	20	7.0341E-4	45.0	19
ACGGGTA	135	0.0	43.333336	5
ATATACG	100	0.0	42.75	1
CGAATAT	350	0.0	42.428574	14
GGTACCT	795	0.0	41.603775	8
CTATACG	65	0.0	41.538464	1
GCTACGA	375	0.0	40.8	10
CGTATGA	50	1.0822987E-9	40.5	15
GGGTACC	875	0.0	39.857143	7
CTACGAA	385	0.0	39.740257	11
TTGATCC	755	0.0	39.63576	17
CATATGC	735	0.0	39.489796	33
TGAATCG	40	3.4593359E-7	39.375	1
TACGAAT	390	0.0	39.23077	12
AGCTACG	385	0.0	39.15584	9
TATAGCG	75	0.0	39.000004	1
ATAGGGT	525	0.0	39.0	4
TAGGGTA	620	0.0	38.830647	5
AACGGGA	420	0.0	38.57143	4
>>END_MODULE