FastQCFastQC Report
Sat 18 Jun 2016
SRR3548964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3428857
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167270.48783020114282977No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101750.29674611685468366No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT76450.22296059590703257No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC56020.1633780586358661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG302.1667656E-645.00000443
AATGCGT207.035245E-445.00000431
ATATACG950.045.01
ATGTACG1000.042.751
TCGATAG750.042.01
TCTAGCG750.042.01
ACCGTAA451.9303116E-840.00000424
TACCGTA451.9303116E-840.00000423
GCTACGA10550.039.8815210
CTAAGCG1250.039.61
CGACGGT3400.039.04411728
CGTTTTT122300.039.020851
TGTTACG1500.039.01
TAAGGGA26400.038.9488644
GGACTAA43750.038.777148
CGTTAGG6400.038.671882
ACGTTAG1400.038.571431
TAATCCG356.251914E-638.5714323
AACGGGA8550.038.4210554
TAGGGTA16350.038.3944975
Produced by FastQC (version 0.11.5)