##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3428857
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.192208657287253	31.0	31.0	33.0	30.0	34.0
2	31.45655768088316	31.0	31.0	34.0	30.0	34.0
3	31.54153118663158	31.0	31.0	34.0	30.0	34.0
4	35.30732252759447	37.0	35.0	37.0	33.0	37.0
5	35.16275423559512	37.0	35.0	37.0	32.0	37.0
6	35.16005158570334	37.0	35.0	37.0	32.0	37.0
7	35.81754357209997	37.0	35.0	37.0	35.0	37.0
8	35.9008765894874	37.0	35.0	37.0	35.0	37.0
9	37.54040661363247	39.0	37.0	39.0	35.0	39.0
10	36.74452915359258	39.0	37.0	39.0	32.0	39.0
11	36.38854813717808	38.0	35.0	39.0	32.0	39.0
12	35.47925241560088	37.0	35.0	39.0	30.0	39.0
13	35.05380568510148	37.0	34.0	39.0	30.0	39.0
14	35.83520689255924	38.0	34.0	40.0	29.0	41.0
15	36.24943909880173	38.0	35.0	40.0	30.0	41.0
16	36.160765526238045	38.0	35.0	40.0	31.0	41.0
17	36.21151363267701	37.0	34.0	40.0	31.0	41.0
18	36.31351351193707	37.0	35.0	40.0	31.0	41.0
19	36.2336140002339	37.0	35.0	40.0	31.0	41.0
20	36.10202262736533	37.0	34.0	40.0	31.0	41.0
21	35.974953461167964	37.0	34.0	40.0	30.0	41.0
22	35.85788179559544	36.0	34.0	40.0	30.0	41.0
23	35.79690783255178	36.0	34.0	40.0	30.0	41.0
24	35.612300250491636	36.0	34.0	40.0	30.0	41.0
25	35.57449727416454	36.0	34.0	40.0	30.0	41.0
26	35.4252355814197	36.0	34.0	40.0	29.0	41.0
27	35.40240027507709	36.0	34.0	40.0	29.0	41.0
28	35.434049888928	36.0	34.0	40.0	29.0	41.0
29	35.429795118314935	36.0	34.0	40.0	29.0	41.0
30	35.301653291461264	36.0	34.0	40.0	29.0	41.0
31	35.18066049415301	36.0	34.0	40.0	29.0	41.0
32	35.0333656375871	36.0	34.0	40.0	29.0	41.0
33	34.857436166046	35.0	34.0	40.0	27.0	41.0
34	34.67227971303557	35.0	34.0	40.0	26.0	41.0
35	34.52777645728591	35.0	33.0	40.0	25.0	41.0
36	34.31936123320395	35.0	33.0	40.0	24.0	41.0
37	34.1642001984918	35.0	33.0	40.0	24.0	41.0
38	34.23682614935531	35.0	33.0	40.0	24.0	41.0
39	34.25018307850109	35.0	33.0	40.0	24.0	41.0
40	34.0791709890497	35.0	33.0	40.0	23.0	41.0
41	34.08232918433169	35.0	33.0	40.0	24.0	41.0
42	33.99758520113262	35.0	33.0	40.0	24.0	41.0
43	33.870236933182106	35.0	33.0	40.0	23.0	41.0
44	33.814243930265974	35.0	33.0	39.0	23.0	41.0
45	33.75430063137658	35.0	33.0	39.0	23.0	41.0
46	33.66089457798911	35.0	33.0	39.0	23.0	41.0
47	33.6787393000058	35.0	33.0	39.0	23.0	41.0
48	33.64405573052478	35.0	33.0	39.0	23.0	41.0
49	33.64196115498547	35.0	33.0	39.0	24.0	41.0
50	33.452703626893744	35.0	32.0	39.0	23.0	41.0
51	33.271997344887815	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	7.0
12	4.0
13	12.0
14	38.0
15	134.0
16	362.0
17	908.0
18	2058.0
19	4049.0
20	6864.0
21	11061.0
22	16507.0
23	23628.0
24	33536.0
25	45757.0
26	58968.0
27	70189.0
28	80435.0
29	93177.0
30	111348.0
31	136803.0
32	168978.0
33	219229.0
34	362736.0
35	510033.0
36	246259.0
37	289398.0
38	389632.0
39	546379.0
40	366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.51927479040391	24.022086660365247	28.03800216806942	14.420636381161419
2	31.86831646814084	25.9462263955598	29.257446431857616	12.928010704441743
3	29.481077805227805	26.043051664155143	31.49760984491333	12.978260685703718
4	26.112812520323832	28.68384420814283	30.73123201113374	14.472111260399604
5	23.232056629949863	32.706729968616365	29.653992569535564	14.40722083189821
6	21.13593538604847	41.352147377391354	26.919291180705407	10.592626055854765
7	86.03382993224857	5.16577390074885	6.8434175003507	1.95697866665189
8	87.15857791678101	4.1614158887349335	6.778789549987066	1.9012166444969854
9	83.20451975687526	5.650512692713637	8.274389978934671	2.8705775714764425
10	51.48806147354643	23.11910353800115	14.332268741449411	11.06056624700301
11	44.62752456576637	21.88446470646049	20.41304143042419	13.074969297348943
12	41.50490965356678	21.483543933153236	24.178057002668822	12.833489410611174
13	21.85153828229057	39.94462294578047	22.943359842653106	15.26047892927585
14	15.325427686252299	41.963663109893474	26.874144941010954	15.836764262843275
15	14.866965872300886	23.361779158477592	47.04448158672117	14.72677338250035
16	16.832664645973864	19.320082464798034	44.815138105788606	19.032114783439496
17	15.286726743051693	19.852679770547446	30.636594060353055	34.223999426047804
18	20.072753106939135	23.93077343266284	35.82047895260724	20.17599450779079
19	30.39056455256081	24.623861537532772	25.724578190341564	19.260995719564857
20	31.987685692345874	23.291026718232928	26.426998851220684	18.294288738200514
21	21.984002249146	30.572491066264938	27.829973661777085	19.613533022811975
22	21.65876267222576	25.897259640749088	24.04016848763305	28.4038091993921
23	19.26434960688066	31.298768073442552	25.86237921266474	23.574503107012045
24	18.647671804335964	23.784339796031155	41.430686669056186	16.13730173057669
25	17.264178704448742	25.874686520901864	37.03808586943113	19.82304890521827
26	16.234010342221914	34.9819487951816	27.412808408166338	21.37123245443015
27	16.325906854674894	36.74854331924603	27.810812757720722	19.114737068358348
28	14.933576990816475	30.78364014597284	37.07716594771961	17.20561691549108
29	16.00218381810615	25.789993575118476	36.14997650820667	22.057846098568707
30	18.79509702504362	33.12272865272597	30.52367013264187	17.558504189588543
31	29.211483593512355	28.436939773224722	24.947817888001744	17.403758745261175
32	31.10844225933015	25.010695984113656	27.01521235793735	16.86564939861884
33	29.46395839779845	27.2871397086551	23.363354027304144	19.885547866242305
34	18.926744393248246	27.17374915314345	27.58053193819398	26.31897451541432
35	20.138635119516504	25.308637834707014	31.64176867101778	22.910958374758703
36	29.725503279955973	28.17043697068732	24.216582960444253	17.88747678891246
37	19.758595940279807	35.721116395346904	27.846568112930925	16.67371955144236
38	18.857712643017777	35.77384533679882	24.826786302257574	20.541655717925828
39	20.8041630199218	34.220995509582345	25.70556310747284	19.269278363023012
40	25.062083370639254	25.38428986685651	26.82491570806248	22.728711054441757
41	16.844126191322648	25.11933276890812	28.53851881253724	29.498022227231992
42	18.991868135649867	25.62337828611692	25.327769574525856	30.05698400370736
43	19.283248032799268	25.34611387993142	27.91154603414491	27.459092053124408
44	17.61438286869356	29.4741658809335	30.49864721684223	22.412804033530705
45	17.318249200826983	38.85673855748431	23.603375702165476	20.22163653952323
46	20.194426305908937	35.614346121754274	24.608287834692437	19.582939737644352
47	19.70312556050019	28.768857960539034	27.1860856256181	24.341930853342674
48	19.912991413756828	25.662954156443384	33.817041655572105	20.60701277422768
49	20.533635552605432	26.817012199692204	32.81341274949641	19.835939498205963
50	18.811108191446888	35.07935734852751	26.275986429296992	19.833548030728608
51	16.81548691006945	34.04475602219632	26.665183179117708	22.47457388861653
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11734.0
1	10692.0
2	9650.0
3	20281.0
4	30912.0
5	22484.0
6	14056.0
7	14055.0
8	14054.0
9	15314.0
10	16574.0
11	17508.5
12	18443.0
13	18434.0
14	18425.0
15	17738.5
16	17052.0
17	16196.0
18	15340.0
19	14845.0
20	14350.0
21	13948.0
22	13546.0
23	16150.0
24	18754.0
25	19532.5
26	26061.0
27	31811.0
28	37687.0
29	43563.0
30	51622.5
31	59682.0
32	72493.5
33	85305.0
34	101328.5
35	117352.0
36	121493.0
37	125634.0
38	145118.5
39	164603.0
40	206828.0
41	249053.0
42	301670.5
43	354288.0
44	345664.0
45	337040.0
46	318024.0
47	299008.0
48	273273.5
49	247539.0
50	231072.0
51	214605.0
52	189357.0
53	164109.0
54	145344.0
55	126579.0
56	120206.0
57	113833.0
58	113333.0
59	112833.0
60	107333.5
61	101834.0
62	88857.5
63	75881.0
64	63740.5
65	51600.0
66	43214.0
67	34828.0
68	31027.5
69	27227.0
70	23488.0
71	19749.0
72	17775.5
73	15802.0
74	13310.0
75	8006.5
76	5195.0
77	4200.5
78	3206.0
79	2359.5
80	1513.0
81	1084.5
82	656.0
83	453.5
84	251.0
85	219.0
86	187.0
87	115.5
88	44.0
89	27.5
90	11.0
91	9.5
92	8.0
93	6.5
94	5.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3428857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.761442948705263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.01097664758349	13.243379773999544
2	7.794687547093614	2.6130042124718593
3	2.7239244993535587	1.3697071527748563
4	1.4424949255803734	0.9671318559564909
5	0.9573230926692732	0.8023058200627051
6	0.7045534191699647	0.7085599163839553
7	0.5070447138694147	0.5949160730775346
8	0.3931502961977991	0.5271813007988788
9	0.3048551879064196	0.4598831555739247
>10	3.488410301589606	14.008237850934782
>50	1.3642196892644705	16.50351442028624
>100	1.275605754179562	42.944056467326014
>500	0.027174960203020706	2.9438629713300064
>1k	0.004859098844248735	1.1031643241711377
>5k	3.599332477221285E-4	0.3995957945447229
>10k+	3.599332477221285E-4	0.8114989103074005
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16727	0.48783020114282977	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10175	0.29674611685468366	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	7645	0.22296059590703257	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5602	0.1633780586358661	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9164237528715837E-5	0.0	0.0	0.03523039893468873	0.0
2	2.9164237528715837E-5	0.0	0.0	0.10989084700820127	0.0
3	2.9164237528715837E-5	0.0	0.0	0.16136572624638473	0.0
4	2.9164237528715837E-5	0.0	0.0	0.22322307404479103	0.0
5	2.9164237528715837E-5	0.0	0.0	0.39255063713651517	0.0
6	2.9164237528715837E-5	0.0	0.0	0.5964378216997676	0.0
7	2.9164237528715837E-5	0.0	0.0	0.7375635671012235	0.0
8	2.9164237528715837E-5	0.0	0.0	1.0445171670909577	0.0
9	2.9164237528715837E-5	0.0	0.0	1.1893759348960893	0.0
10	5.8328475057431674E-5	0.0	0.0	1.413153129453926	0.0
11	5.8328475057431674E-5	0.0	0.0	1.6212399642213133	0.0
12	5.8328475057431674E-5	0.0	0.0	1.8031664779254428	0.0
13	8.749271258614751E-5	0.0	0.0	1.8812099775522864	0.0
14	8.749271258614751E-5	0.0	0.0	1.9137572666343332	0.0
15	8.749271258614751E-5	0.0	0.0	1.9596034480294746	0.0
16	8.749271258614751E-5	0.0	0.0	2.054591369660502	0.0
17	8.749271258614751E-5	0.0	0.0	2.16841938873508	0.0
18	1.1665695011486335E-4	0.0	0.0	2.349062675987946	0.0
19	1.1665695011486335E-4	0.0	0.0	2.42663954781433	0.0
20	1.1665695011486335E-4	0.0	0.0	2.5039247772654267	0.0
21	1.1665695011486335E-4	0.0	0.0	2.6172570043020165	0.0
22	1.1665695011486335E-4	0.0	0.0	2.737413662920326	0.0
23	1.7498542517229503E-4	0.0	0.0	2.885422168378559	0.0
24	2.916423752871584E-4	0.0	0.0	2.999512665590895	0.0
25	2.916423752871584E-4	0.0	0.0	3.088113619203134	0.0
26	3.208066128158742E-4	0.0	0.0	3.173477342449685	0.0
27	3.208066128158742E-4	0.0	0.0	3.2607659053731317	0.0
28	3.4997085034459006E-4	0.0	0.0	3.3570662176929513	0.0
29	4.082993254020217E-4	0.0	0.0	3.46042427549472	0.0
30	4.082993254020217E-4	0.0	0.0	3.612778252344732	0.0
31	4.082993254020217E-4	0.0	0.0	3.7344222870770056	0.0
32	4.082993254020217E-4	0.0	0.0	3.846237973762102	0.0
33	4.082993254020217E-4	0.0	0.0	3.964586449653631	0.0
34	4.082993254020217E-4	0.0	0.0	4.083401553345619	0.0
35	4.082993254020217E-4	0.0	0.0	4.241996677026776	0.0
36	4.957920379881693E-4	0.0	0.0	4.371398398941688	0.0
37	4.957920379881693E-4	0.0	0.0	4.499721044068038	0.0
38	4.957920379881693E-4	0.0	0.0	4.646242173412306	0.0
39	5.832847505743168E-4	0.0	0.0	4.77742291381647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	30	2.1667656E-6	45.000004	43
AATGCGT	20	7.035245E-4	45.000004	31
ATATACG	95	0.0	45.0	1
ATGTACG	100	0.0	42.75	1
TCGATAG	75	0.0	42.0	1
TCTAGCG	75	0.0	42.0	1
ACCGTAA	45	1.9303116E-8	40.000004	24
TACCGTA	45	1.9303116E-8	40.000004	23
GCTACGA	1055	0.0	39.88152	10
CTAAGCG	125	0.0	39.6	1
CGACGGT	340	0.0	39.044117	28
CGTTTTT	12230	0.0	39.02085	1
TGTTACG	150	0.0	39.0	1
TAAGGGA	2640	0.0	38.948864	4
GGACTAA	4375	0.0	38.77714	8
CGTTAGG	640	0.0	38.67188	2
ACGTTAG	140	0.0	38.57143	1
TAATCCG	35	6.251914E-6	38.57143	23
AACGGGA	855	0.0	38.421055	4
TAGGGTA	1635	0.0	38.394497	5
>>END_MODULE