##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1188389
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22549687013259	31.0	31.0	33.0	30.0	34.0
2	31.501865971495864	31.0	31.0	34.0	30.0	34.0
3	31.605487765369755	31.0	31.0	34.0	30.0	34.0
4	35.38706265372702	37.0	35.0	37.0	33.0	37.0
5	35.268104130886435	37.0	35.0	37.0	33.0	37.0
6	35.31999286428939	37.0	35.0	37.0	32.0	37.0
7	35.84914619707857	37.0	35.0	37.0	35.0	37.0
8	35.84639541429616	37.0	35.0	37.0	35.0	37.0
9	37.578770082860075	39.0	37.0	39.0	35.0	39.0
10	36.81587678781948	39.0	37.0	39.0	32.0	39.0
11	36.52119129342328	39.0	35.0	39.0	32.0	39.0
12	35.788285653939916	37.0	35.0	39.0	31.0	39.0
13	35.48422696608602	37.0	35.0	39.0	30.0	39.0
14	36.45112332746264	38.0	35.0	40.0	31.0	41.0
15	36.7220522909586	38.0	35.0	40.0	31.0	41.0
16	36.84428499422327	38.0	35.0	41.0	32.0	41.0
17	36.77305410938674	38.0	35.0	40.0	31.0	41.0
18	36.67449799686803	38.0	35.0	40.0	31.0	41.0
19	36.62665675969737	38.0	35.0	40.0	31.0	41.0
20	36.52209587937956	38.0	35.0	40.0	31.0	41.0
21	36.38388187706214	38.0	35.0	40.0	31.0	41.0
22	36.284255407951434	38.0	35.0	40.0	30.0	41.0
23	36.250003155532404	38.0	35.0	40.0	31.0	41.0
24	36.16581186799945	38.0	35.0	40.0	30.0	41.0
25	36.04899069244161	37.0	34.0	40.0	30.0	41.0
26	35.906077892003374	37.0	34.0	40.0	30.0	41.0
27	35.81433352210429	37.0	34.0	40.0	30.0	41.0
28	35.81455987896219	37.0	34.0	40.0	30.0	41.0
29	35.82411146518522	37.0	34.0	40.0	30.0	41.0
30	35.74876744904236	37.0	34.0	40.0	30.0	41.0
31	35.61189223394023	37.0	34.0	40.0	29.0	41.0
32	35.479027490156845	37.0	34.0	40.0	29.0	41.0
33	35.290502520639286	37.0	34.0	40.0	28.0	41.0
34	35.127245371675436	37.0	34.0	40.0	27.0	41.0
35	34.987334955136745	37.0	34.0	40.0	27.0	41.0
36	34.82688076042441	37.0	33.0	40.0	26.0	41.0
37	34.7571872509759	37.0	33.0	40.0	26.0	41.0
38	34.71812260126945	36.0	33.0	40.0	26.0	41.0
39	34.64775927747564	36.0	33.0	40.0	26.0	41.0
40	34.489901875564314	36.0	33.0	40.0	25.0	41.0
41	34.44909453049465	36.0	33.0	40.0	25.0	41.0
42	34.4040461498718	36.0	33.0	40.0	25.0	41.0
43	34.34160784053033	35.0	33.0	40.0	25.0	41.0
44	34.199866373721065	35.0	33.0	40.0	24.0	41.0
45	34.1129133642267	35.0	33.0	40.0	24.0	41.0
46	34.04326697739545	35.0	33.0	39.0	24.0	41.0
47	33.96807695123398	35.0	33.0	39.0	24.0	41.0
48	33.89399851395461	35.0	33.0	39.0	24.0	41.0
49	33.865549916736015	35.0	33.0	39.0	24.0	41.0
50	33.79208828085753	35.0	33.0	39.0	24.0	41.0
51	33.63485441214956	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	7.0
15	38.0
16	95.0
17	248.0
18	534.0
19	1100.0
20	1908.0
21	3203.0
22	4633.0
23	7076.0
24	9858.0
25	13855.0
26	18577.0
27	22122.0
28	25576.0
29	29183.0
30	35428.0
31	43418.0
32	54471.0
33	70827.0
34	114158.0
35	153929.0
36	95913.0
37	117917.0
38	154388.0
39	209782.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.63259000209528	23.4328153491828	28.935811422017537	13.998783226704386
2	31.12793874732937	26.0850613730016	30.1355027688745	12.65149711079453
3	28.691699435117624	25.918449261984083	32.199809994875416	13.190041308022879
4	25.938897111972597	27.586842355491342	31.394854715080665	15.079405817455397
5	23.615752081178805	32.99466757097213	29.66537051420032	13.724209833648745
6	21.013489690665264	41.717568910516675	27.053094567519558	10.215846831298506
7	88.66852520513065	3.9551863909881355	6.086727494111777	1.2895609097694443
8	89.95110186984229	2.8449438693895686	5.827384804134	1.3765694566341493
9	85.92338030728996	5.05129212740946	6.827141617769938	2.1981859475306487
10	47.22367844199164	28.321786889646404	12.747761886049098	11.706772782312862
11	42.23877871639674	21.44045426203036	20.655273651977595	15.66549336959531
12	37.84610931269138	20.735550396376944	25.390507653638668	16.027832637293006
13	24.03388116180813	33.131996341265356	25.45757323569976	17.376549261226753
14	18.60939473522559	35.75369681139762	28.463743774134564	17.173164679242234
15	17.602317086408576	22.552716324368536	41.5392602927156	18.30570629650729
16	19.69725401362685	19.475609417455058	39.92934973312611	20.897786835791983
17	20.338374050921036	19.4027376557676	28.175538481086583	32.08334981222479
18	23.76494565331722	22.509127903405364	31.960915154886155	21.76501128839126
19	29.132043463882617	23.534128976286382	24.92424618538206	22.40958137444894
20	30.161672650958565	23.08006889999823	25.226840706199738	21.531417742843463
21	24.653543578743996	25.78095219662922	28.234610047720064	21.33089417690672
22	25.04146369581004	22.743815366853784	26.08565040571732	26.129070531618854
23	22.20148453073867	26.746629260284298	25.528088866524346	25.52379734245268
24	22.668419179241813	23.23582597954037	34.15640838143066	19.939346459787156
25	21.77948466369177	23.83318930080975	32.169685178842954	22.217640856655525
26	20.172771710273317	29.744300898106594	26.346087013595714	23.736840378024368
27	20.476880886645702	29.86370624433582	27.909548136174266	21.749864732844213
28	19.65997665747495	26.70421890475257	34.635376126840626	19.00042831093186
29	20.760458065498753	24.0417068821741	32.28850149235646	22.909333559970683
30	21.873056717960196	27.68335957333836	29.61042217657686	20.833161532124585
31	29.18648691632117	25.587076285627013	24.466820207861232	20.759616590190586
32	29.726798211696675	24.743328994125662	26.13689625198483	19.392976542192834
33	28.915784309683108	25.482228462229116	24.53885049424052	21.06313673384725
34	22.079302315992493	27.11191369156059	27.220800596437698	23.587983396009218
35	23.449224117692104	24.259228249335866	28.823222025784485	23.468325607187545
36	30.1399625880078	24.96480529523582	24.97767986745081	19.917552249305572
37	22.978082092648116	30.646530723525718	26.669886712179263	19.70550047164691
38	23.299862250492055	30.82778450490538	23.411610171416935	22.460743073185633
39	23.26653982828855	28.879180133777744	25.150687190810416	22.703592847123293
40	24.549957968308355	25.694953420134315	25.57176143501833	24.183327176538995
41	20.243623931221176	24.528836938073308	27.078338826764636	28.14920030394088
42	22.905210330960653	25.81595756944906	24.162795179019664	27.116036920570618
43	23.607253180566296	25.541804914047507	25.632515952268154	25.218425953118047
44	21.994734047521476	26.486108504875087	27.72501260109274	23.794144846510694
45	20.019454909124875	32.17565965353096	24.539860264610326	23.265025172733843
46	23.598922575015422	29.67302793950466	26.635806962198405	20.092242523281516
47	23.86196775634914	26.690923594883493	26.541056842498545	22.906051806268824
48	23.106322929613114	24.69098922995753	29.43489042729275	22.76779741313661
49	23.215462277082672	24.71707496451078	29.79100277770999	22.276459980696554
50	22.124573687572	29.650308106184088	26.492167127093907	21.73295107915001
51	21.43649932808197	30.32887379469181	24.790535758913958	23.44409111831227
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	643.0
1	1269.5
2	1896.0
3	5621.0
4	9346.0
5	6896.0
6	4446.0
7	4707.0
8	4968.0
9	5247.5
10	5527.0
11	5521.0
12	5515.0
13	5277.5
14	5040.0
15	4771.5
16	4503.0
17	4245.0
18	3987.0
19	3671.5
20	3356.0
21	3632.5
22	3909.0
23	3919.0
24	3929.0
25	4533.5
26	5829.5
27	6521.0
28	9070.0
29	11619.0
30	13699.5
31	15780.0
32	18509.0
33	21238.0
34	24066.5
35	26895.0
36	28795.0
37	30695.0
38	37144.0
39	43593.0
40	53829.5
41	64066.0
42	78567.5
43	93069.0
44	93841.0
45	94613.0
46	93953.5
47	93294.0
48	89898.0
49	86502.0
50	83374.5
51	80247.0
52	75796.0
53	71345.0
54	67171.5
55	62998.0
56	64261.5
57	65525.0
58	61844.0
59	58163.0
60	55454.0
61	52745.0
62	47763.5
63	42782.0
64	38059.5
65	33337.0
66	28621.0
67	23905.0
68	20266.5
69	16628.0
70	14676.5
71	12725.0
72	10429.0
73	8133.0
74	6597.0
75	3737.5
76	2414.0
77	1770.5
78	1127.0
79	903.5
80	680.0
81	484.5
82	289.0
83	208.0
84	127.0
85	79.5
86	32.0
87	24.0
88	16.0
89	14.5
90	13.0
91	10.5
92	8.0
93	4.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1188389.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.598122693068476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68844314540436	13.671707543063006
2	6.568353856010327	2.311813940991183
3	2.4510523105630315	1.2940175786525163
4	1.3963018941912384	0.9828916820216532
5	0.9596918825184813	0.8444387748051045
6	0.7286839518050251	0.7694081753000898
7	0.6018469342836568	0.7413963334379639
8	0.49861717576942166	0.7019780988849258
9	0.45289620673369974	0.7173110711992456
>10	5.643440073600892	23.26326908543468
>50	1.7890683502855549	22.742731538594757
>100	1.2168154919867014	30.982232440780272
>500	0.0038309814781163362	0.4340881760361524
>1k	4.7887268476454203E-4	0.11730746283096953
>5k	4.7887268476454203E-4	0.42540809796748147
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5048	0.42477673556386	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1392	0.11713336289716583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.026254029614881994	0.0
2	0.0	0.0	0.0	0.09920993883315984	0.0
3	0.0	0.0	0.0	0.13859098325548283	0.0
4	0.0	0.0	0.0	0.1906783048311622	0.0
5	0.0	0.0	0.0	0.29199193193474526	0.0
6	0.0	0.0	0.0	0.389014034966665	0.0
7	0.0	0.0	0.0	0.44615020839136005	0.0
8	0.0	0.0	0.0	0.6000560422555241	0.0
9	0.0	0.0	0.0	0.6684679848096877	0.0
10	0.0	0.0	0.0	0.7951941662199835	0.0
11	0.0	0.0	0.0	0.9178812661510667	0.0
12	0.0	0.0	0.0	1.0167546148609587	0.0
13	0.0	0.0	0.0	1.0581552000228882	0.0
14	0.0	0.0	0.0	1.0780140172956836	0.0
15	0.0	0.0	0.0	1.1066241777734396	0.0
16	0.0	0.0	0.0	1.170492153663489	0.0
17	0.0	0.0	0.0	1.245804193744641	0.0
18	0.0	0.0	0.0	1.3497263943035487	0.0
19	0.0	0.0	0.0	1.3933989627975352	0.0
20	0.0	0.0	0.0	1.4427935633870728	0.0
21	8.414753081692948E-5	0.0	0.0	1.5049785886607836	0.0
22	8.414753081692948E-5	0.0	0.0	1.5697721873898194	0.0
23	8.414753081692948E-5	0.0	0.0	1.641718326238294	0.0
24	8.414753081692948E-5	0.0	0.0	1.6983496144780876	0.0
25	1.6829506163385896E-4	0.0	0.0	1.7423587730953416	0.0
26	1.6829506163385896E-4	0.0	0.0	1.7912484884999778	0.0
27	1.6829506163385896E-4	0.0	0.0	1.8477956292089543	0.0
28	1.6829506163385896E-4	0.0	0.0	1.8941609186890824	0.0
29	1.6829506163385896E-4	0.0	0.0	1.9490251087817205	0.0
30	1.6829506163385896E-4	0.0	0.0	2.0169321661509825	0.0
31	1.6829506163385896E-4	0.0	0.0	2.0841660432737092	0.0
32	1.6829506163385896E-4	0.0	0.0	2.1410497741059533	0.0
33	1.6829506163385896E-4	0.0	0.0	2.201299406170875	0.0
34	1.6829506163385896E-4	0.0	0.0	2.2630636937905013	0.0
35	1.6829506163385896E-4	0.0	0.0	2.343256290659035	0.0
36	1.6829506163385896E-4	0.0	0.0	2.409143807288691	0.0
37	1.6829506163385896E-4	0.0	0.0	2.4731800782403743	0.0
38	1.6829506163385896E-4	0.0	0.0	2.5469774627668214	0.0
39	1.6829506163385896E-4	0.0	0.0	2.6211114374165363	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	3.890885E-5	45.000004	21
CTATACG	30	2.1655633E-6	45.000004	1
TAGAACG	35	1.2119744E-7	45.000004	1
ACTTCGC	25	3.890885E-5	45.000004	11
TAACGCG	25	3.890885E-5	45.000004	1
TATGCGT	30	2.1655633E-6	45.000004	20
TCGATAG	45	3.8562575E-10	45.000004	1
CCGCTAA	25	3.890885E-5	45.000004	31
TGTTACG	35	1.2119744E-7	45.000004	1
ACTCGAT	25	3.890885E-5	45.000004	19
CGGTCCA	30	2.1655633E-6	45.000004	24
TATCGTC	25	3.890885E-5	45.000004	14
ATCGCGA	30	2.1655633E-6	45.000004	27
TACACGG	65	0.0	45.000004	2
TACGTAA	25	3.890885E-5	45.000004	39
AATCACG	20	7.033383E-4	45.0	1
ACGCATT	20	7.033383E-4	45.0	40
AGTATCG	20	7.033383E-4	45.0	1
TTATACG	40	6.8157533E-9	45.0	36
ACGATTT	20	7.033383E-4	45.0	37
>>END_MODULE