##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548959_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1181048
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.983230994845258	31.0	31.0	33.0	28.0	34.0
2	31.25175522078696	31.0	31.0	34.0	28.0	34.0
3	31.36534586231889	31.0	31.0	34.0	28.0	34.0
4	35.14271477535206	35.0	35.0	37.0	32.0	37.0
5	34.99126199781889	35.0	35.0	37.0	32.0	37.0
6	35.04058768144902	37.0	35.0	37.0	32.0	37.0
7	35.68905582160928	37.0	35.0	37.0	35.0	37.0
8	35.6864445814226	37.0	35.0	37.0	35.0	37.0
9	37.34920511274732	39.0	37.0	39.0	35.0	39.0
10	36.54856195514492	39.0	35.0	39.0	32.0	39.0
11	36.17412332098272	38.0	35.0	39.0	31.0	39.0
12	35.304526149656915	37.0	35.0	39.0	30.0	39.0
13	34.874887388150185	37.0	33.0	39.0	27.0	39.0
14	35.802633762556646	38.0	34.0	40.0	27.0	41.0
15	36.16686705366759	38.0	34.0	40.0	30.0	41.0
16	36.33908274684856	38.0	35.0	40.0	31.0	41.0
17	36.28098095928362	38.0	34.0	40.0	31.0	41.0
18	36.23096182373621	37.0	35.0	40.0	30.0	41.0
19	36.149541762908875	37.0	35.0	40.0	30.0	41.0
20	35.99105201482074	37.0	34.0	40.0	30.0	41.0
21	35.841644878108255	37.0	34.0	40.0	30.0	41.0
22	35.72720414411607	37.0	34.0	40.0	30.0	41.0
23	35.67385322188429	37.0	34.0	40.0	30.0	41.0
24	35.588097181486276	36.0	34.0	40.0	29.0	41.0
25	35.530340003115874	36.0	34.0	40.0	29.0	41.0
26	35.354014400769486	36.0	34.0	40.0	29.0	41.0
27	35.246553061348905	36.0	34.0	40.0	29.0	41.0
28	35.23854237931058	36.0	34.0	40.0	29.0	41.0
29	35.25228102498798	36.0	34.0	40.0	29.0	41.0
30	35.179333100771515	36.0	34.0	40.0	29.0	41.0
31	35.02187802697266	36.0	34.0	40.0	27.0	41.0
32	34.84177696418774	36.0	34.0	40.0	27.0	41.0
33	34.64166486036131	36.0	33.0	40.0	26.0	41.0
34	34.52233101448883	36.0	33.0	40.0	25.0	41.0
35	34.37632001408918	36.0	33.0	40.0	25.0	41.0
36	34.16802873380252	35.0	33.0	40.0	23.0	41.0
37	34.13252636641356	35.0	33.0	40.0	23.0	41.0
38	34.10139554023206	35.0	33.0	40.0	24.0	41.0
39	34.03578178024941	35.0	33.0	40.0	23.0	41.0
40	33.91375625715466	35.0	33.0	40.0	23.0	41.0
41	33.89478412393061	35.0	33.0	40.0	23.0	41.0
42	33.81528523819523	35.0	33.0	40.0	23.0	41.0
43	33.73819607670476	35.0	33.0	40.0	23.0	41.0
44	33.64420582398006	35.0	32.0	39.0	23.0	41.0
45	33.576272090550084	35.0	32.0	39.0	23.0	41.0
46	33.50726727448842	35.0	32.0	39.0	23.0	41.0
47	33.41735306270363	35.0	32.0	39.0	23.0	41.0
48	33.36676578767332	35.0	32.0	39.0	23.0	41.0
49	33.35537929025747	35.0	32.0	39.0	23.0	41.0
50	33.24454721569318	35.0	32.0	39.0	23.0	41.0
51	33.085064281892016	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	13.0
15	54.0
16	137.0
17	375.0
18	882.0
19	1654.0
20	2881.0
21	4411.0
22	6619.0
23	9778.0
24	13213.0
25	17628.0
26	22653.0
27	26115.0
28	29815.0
29	34261.0
30	40268.0
31	48292.0
32	59565.0
33	74443.0
34	117347.0
35	161449.0
36	87393.0
37	103511.0
38	134461.0
39	183698.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.61666926323062	23.912406608368162	28.970456746889205	13.500467381512014
2	30.40909429591346	27.252406337422357	29.038108527341816	13.300390839322365
3	28.491560038203357	25.8831986506899	31.778979347156085	13.84626196395066
4	26.11672006556888	28.447785356734016	31.017452296604368	14.418042281092724
5	23.515386334848372	32.80340849821514	29.890063740000407	13.791141426936077
6	20.07970886873353	41.75740528750737	26.79967283294159	11.36321301081751
7	86.64093245998468	4.720807283023213	6.8385027534867335	1.7997575035053615
8	87.7079509046203	3.93819726209265	6.199324667583367	2.15452716570368
9	82.98519619863038	5.489531331495417	8.047005710182821	3.4782667596913925
10	49.65589882883676	25.37890077287291	13.437811164321856	11.527389233968476
11	44.42164924710934	20.768419234442632	20.97171325805556	13.838218260392466
12	39.24616103663865	21.49252189580779	24.452181452404982	14.80913561514858
13	22.131191958328536	38.5400932053566	24.92989277319804	14.398822063116826
14	16.7877173493372	40.94177374670632	26.948185001795018	15.32232390216147
15	14.93918960110004	22.9826391476045	46.06468153707554	16.013489714219915
16	16.616344128265744	18.572826845310267	43.678072356076974	21.132756670347014
17	16.583237937831484	20.317125129545964	28.52906909795368	34.57056783466887
18	21.294646788276175	22.485792279399313	34.36278627117611	21.856774661148403
19	28.68587898205661	24.28698918248877	24.358959161693683	22.66817267376093
20	30.62051669364835	23.74873840859982	24.8298968373851	20.800848060366725
21	22.751488508511088	29.295083688385233	26.413321050456883	21.5401067526468
22	23.211673022603655	25.127429198474577	24.707039849354132	26.953857929567636
23	20.131188571505984	30.411549742262807	24.562930549816773	24.894331136414436
24	19.3170811008528	24.384529671952368	37.85629373234619	18.442095494848644
25	18.294938055015546	24.988569473890983	34.6281438180328	22.08834865306067
26	17.406235817680567	33.8209793336088	26.45523297952327	22.317551869187366
27	18.786450677703193	33.274092162215254	27.265530274806782	20.673926885274774
28	16.69669649328393	27.836887239129993	36.66895841659272	18.797457850993354
29	17.080338817727984	24.57292167634169	35.913612317196254	22.433127188734073
30	19.18279358671282	30.890615792076193	29.943236854048266	19.983353767162722
31	29.397789082238823	26.63295649287751	23.459503762759855	20.509750662123807
32	30.076677662550548	25.834682417649407	24.983658581192298	19.104981338607747
33	27.988278207151613	27.00025739851386	24.221708177821732	20.789756216512792
34	18.850461623913677	27.34232647614661	27.698281526237718	26.108930373701998
35	20.47867656522004	25.382033583732415	30.516541241338203	23.622748609709344
36	29.21405395885688	26.07489280706627	24.98204984048066	19.729003393596194
37	21.12285021438587	32.239587214067505	26.53092846353408	20.106634108012543
38	20.80669032926689	32.90408179853825	23.367974883323964	22.9212529888709
39	20.95435579248261	31.366633701593837	25.50963212333453	22.169378382589024
40	24.460987191037113	25.61284554057075	24.683755444317253	25.24241182407489
41	17.71951690363135	24.656406852219384	26.513655668524905	31.11042057562436
42	21.844836111656765	25.747217725274503	24.38893254126843	28.019013621800298
43	22.045081994973955	26.295882978507223	25.216333290433578	26.44270173608524
44	20.30459388610793	29.661114535565027	28.284117156965678	21.75017442136137
45	18.094861512825897	35.97482913480231	23.318527274082	22.611782078289792
46	21.91087915139774	32.96902412095021	25.09999593581294	20.020100791839113
47	21.46771342062304	28.07269475923079	25.890734330865467	24.568857489280706
48	21.90740765828315	25.16476891709736	30.664968739627856	22.262854684991634
49	21.196174922611107	24.328562429300078	31.45816258102973	23.017100067059086
50	19.416230331028036	32.00504975242327	26.206301522038057	22.37241839451064
51	18.665371771511406	33.19653392580149	24.08081635970765	24.057277942979454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	711.0
1	1005.0
2	1299.0
3	4911.5
4	8524.0
5	6261.0
6	3998.0
7	4383.5
8	4769.0
9	5088.0
10	5407.0
11	5578.5
12	5750.0
13	5692.0
14	5634.0
15	5293.0
16	4952.0
17	4731.0
18	4510.0
19	4250.5
20	3991.0
21	4237.5
22	4484.0
23	4369.5
24	4255.0
25	5065.0
26	7327.5
27	8780.0
28	11761.0
29	14742.0
30	15159.0
31	15576.0
32	19049.5
33	22523.0
34	25409.5
35	28296.0
36	31605.5
37	34915.0
38	41936.0
39	48957.0
40	60072.0
41	71187.0
42	91649.5
43	112112.0
44	112661.5
45	113211.0
46	109873.0
47	106535.0
48	101908.0
49	97281.0
50	89735.0
51	82189.0
52	76888.5
53	71588.0
54	65033.5
55	58479.0
56	55994.0
57	53509.0
58	49910.5
59	46312.0
60	41948.5
61	37585.0
62	32302.5
63	27020.0
64	23836.0
65	20652.0
66	17550.0
67	14448.0
68	12038.0
69	9628.0
70	8660.0
71	7692.0
72	6484.0
73	5276.0
74	4769.0
75	3307.5
76	2353.0
77	1657.0
78	961.0
79	698.0
80	435.0
81	302.5
82	170.0
83	173.5
84	177.0
85	95.5
86	14.0
87	13.5
88	13.0
89	9.5
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1181048.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.230239309681444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.42258347819657	16.067378703570682
2	7.541554960911453	3.0513492325270795
3	2.741900045640779	1.664078822596183
4	1.5077523605335132	1.2200876429332026
5	0.9653551915928988	0.9764683272383862
6	0.6424381796289189	0.7798006869341486
7	0.4950802180663078	0.7010913902279478
8	0.3907016620327503	0.6323190497290255
9	0.32105035627410133	0.5845432984095202
>10	3.6568713319137593	17.406409743985243
>50	1.1686698712226475	16.923058763580883
>100	1.1414330329135733	38.73253905637576
>500	0.0037712545140316297	0.481241695260226
>1k	4.190282793368477E-4	0.33340207635343827
>5k	4.190282793368477E-4	0.446231510278286
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5264	0.4457058476878163	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3933	0.33300932730930494	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03810175369671681	0.0
2	0.0	0.0	0.0	0.13471086695883655	0.0
3	0.0	0.0	0.0	0.19050876848358408	0.0
4	0.0	0.0	0.0	0.28838794020226105	0.0
5	0.0	0.0	0.0	0.5042132072532192	0.0
6	0.0	0.0	0.0	0.751789935718108	0.0
7	0.0	0.0	0.0	0.9352710474087421	0.0
8	0.0	0.0	0.0	1.4678488935250726	0.0
9	0.0	0.0	0.0	1.709752694217339	0.0
10	8.467056377048181E-5	0.0	0.0	2.1003380048905718	0.0
11	8.467056377048181E-5	0.0	0.0	2.3475760511003787	0.0
12	8.467056377048181E-5	0.0	0.0	2.6120022217555934	0.0
13	8.467056377048181E-5	0.0	0.0	2.7114901341859095	0.0
14	8.467056377048181E-5	0.0	0.0	2.748321829426069	0.0
15	8.467056377048181E-5	0.0	0.0	2.798023450359342	0.0
16	8.467056377048181E-5	0.0	0.0	2.9226585202294912	0.0
17	8.467056377048181E-5	0.0	0.0	3.061433574249311	0.0
18	1.6934112754096362E-4	0.0	0.0	3.286403262187481	0.0
19	1.6934112754096362E-4	0.0	0.0	3.3739526251261593	0.0
20	1.6934112754096362E-4	0.0	0.0	3.4692070093679512	0.0
21	1.6934112754096362E-4	0.0	0.0	3.581818859182692	0.0
22	1.6934112754096362E-4	0.0	0.0	3.7002729778975962	0.0
23	2.5401169131144543E-4	0.0	0.0	3.8244846949488926	0.0
24	2.5401169131144543E-4	0.0	0.0	3.922025184412488	0.0
25	2.5401169131144543E-4	0.0	0.0	4.00491766634379	0.0
26	2.5401169131144543E-4	0.0	0.0	4.089672900678042	0.0
27	2.5401169131144543E-4	0.0	0.0	4.172565382609344	0.0
28	4.2335281885240905E-4	0.0	0.0	4.25799798145376	0.0
29	4.2335281885240905E-4	0.0	0.0	4.3535910479506335	0.0
30	4.2335281885240905E-4	0.0	0.0	4.491265384641437	0.0
31	4.2335281885240905E-4	0.0	0.0	4.603030528818473	0.0
32	4.2335281885240905E-4	0.0	0.0	4.702179758993707	0.0
33	4.2335281885240905E-4	0.0	0.0	4.798280848873204	0.0
34	4.2335281885240905E-4	0.0	0.0	4.905812464861716	0.0
35	4.2335281885240905E-4	0.0	0.0	5.043486801552519	0.0
36	4.2335281885240905E-4	0.0	0.0	5.1569453570049655	0.0
37	4.2335281885240905E-4	0.0	0.0	5.265408349194952	0.0
38	4.2335281885240905E-4	0.0	0.0	5.373363318002316	0.0
39	4.2335281885240905E-4	0.0	0.0	5.491140072207057	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	35	1.2119744E-7	45.000004	6
CTATGCG	25	3.8908707E-5	45.000004	1
GTACCTA	25	3.8908707E-5	45.000004	9
CGAACCT	35	1.2119744E-7	45.000004	37
CTCCGAT	30	2.1655524E-6	45.000004	29
CGTTCGA	30	2.1655524E-6	45.000004	14
CCGAGTA	25	3.8908707E-5	45.000004	39
TGTTACG	35	1.2119744E-7	45.000004	1
TTACGCG	55	1.8189894E-12	45.000004	1
TACTACG	25	3.8908707E-5	45.000004	1
ACGTAAC	50	2.1827873E-11	45.000004	13
TAGTGCG	55	1.8189894E-12	45.000004	1
CGCAACA	35	1.2119744E-7	45.000004	30
TCGACAG	25	3.8908707E-5	45.000004	1
TCGAATC	30	2.1655524E-6	45.000004	32
TAGTACG	35	1.2119744E-7	45.000004	1
TCACGAC	335	0.0	45.0	25
TCGCACA	20	7.033367E-4	45.0	40
GTACACG	40	6.8157533E-9	45.0	1
CGAGTGT	20	7.033367E-4	45.0	19
>>END_MODULE