##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548957_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302565
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.976188520342554	31.0	31.0	33.0	28.0	34.0
2	31.246309397227776	31.0	31.0	34.0	28.0	34.0
3	31.38240548456315	31.0	31.0	34.0	28.0	34.0
4	35.17419399415768	35.0	35.0	37.0	32.0	37.0
5	35.02529086840196	35.0	35.0	37.0	32.0	37.0
6	35.073908019945264	37.0	35.0	37.0	32.0	37.0
7	35.66552686430236	37.0	35.0	37.0	35.0	37.0
8	35.66127448534238	37.0	35.0	37.0	35.0	37.0
9	37.34797649253588	39.0	37.0	39.0	35.0	39.0
10	36.53958842744892	39.0	35.0	39.0	32.0	39.0
11	36.19333699277963	38.0	35.0	39.0	31.0	39.0
12	35.3631089427399	37.0	35.0	39.0	30.0	39.0
13	34.977267929047684	37.0	34.0	39.0	28.0	39.0
14	35.88463608342002	38.0	34.0	40.0	28.0	41.0
15	36.22747502044044	38.0	35.0	40.0	30.0	41.0
16	36.40030017695854	38.0	35.0	40.0	31.0	41.0
17	36.34012506093746	38.0	35.0	40.0	31.0	41.0
18	36.28831421080714	38.0	35.0	40.0	30.0	41.0
19	36.21002790647684	37.0	35.0	40.0	30.0	41.0
20	36.05102317350766	37.0	34.0	40.0	30.0	41.0
21	35.90257837420781	37.0	34.0	40.0	30.0	41.0
22	35.77312456576063	37.0	34.0	40.0	30.0	41.0
23	35.72254820296876	37.0	34.0	40.0	30.0	41.0
24	35.67561004633166	37.0	34.0	40.0	29.0	41.0
25	35.59178160015047	37.0	34.0	40.0	29.0	41.0
26	35.41612741014844	36.0	34.0	40.0	29.0	41.0
27	35.30370691673736	36.0	34.0	40.0	29.0	41.0
28	35.29149101964202	36.0	34.0	40.0	29.0	41.0
29	35.29390932506247	36.0	34.0	40.0	29.0	41.0
30	35.24756921919444	36.0	34.0	40.0	29.0	41.0
31	35.10305359041583	36.0	34.0	40.0	27.0	41.0
32	34.90664189503019	36.0	34.0	40.0	27.0	41.0
33	34.697817767251536	36.0	33.0	40.0	26.0	41.0
34	34.567709864766826	36.0	33.0	40.0	25.0	41.0
35	34.437018498117176	36.0	33.0	40.0	25.0	41.0
36	34.23309163074396	36.0	33.0	40.0	24.0	41.0
37	34.15669314007362	36.0	33.0	40.0	23.0	41.0
38	34.137192385792645	35.0	33.0	40.0	24.0	41.0
39	34.04566298035031	35.0	33.0	40.0	23.0	41.0
40	33.91503456641319	35.0	33.0	40.0	23.0	41.0
41	33.85820899532845	35.0	33.0	40.0	23.0	41.0
42	33.77535094217947	35.0	33.0	40.0	23.0	41.0
43	33.700160068787355	35.0	33.0	40.0	23.0	41.0
44	33.581613969360454	35.0	32.0	39.0	23.0	41.0
45	33.51533781423576	35.0	32.0	39.0	23.0	41.0
46	33.41329300265246	35.0	32.0	39.0	23.0	41.0
47	33.319552575111416	35.0	32.0	39.0	23.0	41.0
48	33.288460844564376	35.0	32.0	39.0	23.0	41.0
49	33.277711285041434	35.0	32.0	39.0	23.0	41.0
50	33.21672315776948	35.0	32.0	39.0	23.0	41.0
51	33.01612510700042	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	3.0
14	15.0
15	49.0
16	179.0
17	456.0
18	1001.0
19	1951.0
20	3230.0
21	5228.0
22	7718.0
23	11102.0
24	15075.0
25	19681.0
26	24825.0
27	28712.0
28	32468.0
29	36992.0
30	44005.0
31	52955.0
32	64437.0
33	80632.0
34	127122.0
35	171733.0
36	98404.0
37	117344.0
38	152002.0
39	205112.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.01437548222162	24.408148537692938	26.976465665820896	13.601010314264547
2	29.376729760127134	27.51317592596147	29.89255814489104	13.217536169020356
3	27.700038001942325	26.350470034124978	31.47505114907893	14.47444081485377
4	25.843854241439008	27.64445536307209	31.70905098785857	14.80263940763033
5	22.606011984046862	33.620663844030815	29.53771980668911	14.235604365233211
6	20.266397454253724	40.897920641196414	27.19119583283752	11.644486071712352
7	86.09052139432582	5.081128388986346	6.819851600495945	2.0084986161918983
8	87.7484041103515	4.173688069309401	5.891836491844937	2.1860713284941635
9	83.60319830488305	5.740366123763498	7.405388598649587	3.2510469727038576
10	48.33670488612852	27.474483039234126	13.018620951737534	11.170191122899817
11	43.89830833777969	20.6892554306311	20.960335952524442	14.45210027906477
12	39.25047886285905	20.817464003715745	24.0244440776468	15.907613055778405
13	23.044224280554136	37.275145578147736	23.726186409123535	15.954443732174594
14	16.912399765078902	38.44775500646801	27.385965383685267	17.253879844767823
15	15.030881376361258	22.641864321550173	43.833206020428925	18.494048281659648
16	16.925374165588668	19.075977014582765	41.5960048059022	22.402644013926366
17	17.24704717231002	20.44965126500405	28.503068944735965	33.800232617949966
18	21.983317531178866	23.04806286058661	32.45258394014886	22.516035668085664
19	27.70855964961442	25.147305508746204	24.085170413760544	23.058964427878838
20	30.67486075550932	23.177653322482946	24.887894270151588	21.259591651856148
21	22.76792328981663	28.43489576335922	26.233930744339055	22.563250202485097
22	22.915324763063648	24.71776840311232	24.266428162893984	28.100478670930052
23	20.27399784271802	29.719131099023848	24.155032570351576	25.851838487906555
24	19.877318982162116	23.579782966684963	37.19891137870279	19.34398667245013
25	18.343192086383407	25.36848449021738	33.8322463754208	22.45607704797841
26	18.313174390529454	32.705008963084374	26.577023027641616	22.404793618744552
27	17.89139121656117	33.542049724965736	26.938540495100057	21.628018563373036
28	17.59912173288857	28.099787726524205	35.033491610783344	19.267598929803885
29	17.49970251004748	23.559515264113497	34.75028117598738	24.19050104985164
30	19.645775834603263	30.219374848855914	29.535186343867675	20.59966297267315
31	28.763017584535127	26.47530065678104	23.482206262259464	21.279475496424364
32	29.533957998257282	25.53861035725664	24.68229992361226	20.245131720873815
33	27.881679609079008	26.843497253495986	23.77969621477623	21.495126922648776
34	19.245795795219433	27.63462859818896	26.507621500654476	26.61195410593713
35	20.968089884190043	25.604096532610654	29.554686330432645	23.873127252766658
36	28.69346251434669	26.15769654489411	24.236103380637434	20.912737560121762
37	20.74307232268639	33.11473899575069	26.139885533543435	20.002303148019486
38	20.567956301604912	32.06749759129103	24.312184036881078	23.052362070222983
39	20.652788920322596	32.32529662627201	25.360193157347233	21.66172129605816
40	24.153573909939237	26.860310234038227	25.052722896746037	23.933392959276503
41	19.57407115959664	24.775884504803983	26.45741287382971	29.192631461769665
42	21.57067017768787	25.785431053344748	23.6870328927923	28.956865876175087
43	21.674311838564677	26.148560724416825	25.11590592408056	27.061221512937934
44	21.314329803119232	28.107234571787203	27.330152430013086	23.248283195080475
45	18.047928510285473	35.5493967671479	23.290430803837044	23.112243918729583
46	21.41467028516811	31.84424577660232	25.273057390610067	21.468026547619505
47	20.839881311105398	27.635319542594804	26.362599947027594	25.162199199272205
48	21.61888274289575	24.324237178183047	30.65873871937293	23.398141359548276
49	21.274792428784746	25.299850679236734	30.442089262340076	22.983267629638444
50	20.418558766741008	30.740116616061385	27.323703615558532	21.51762100163907
51	19.246947369229176	32.157780993654825	24.25959549043618	24.33567614667982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1057.0
1	1396.0
2	1735.0
3	4918.0
4	8101.0
5	6236.5
6	4372.0
7	4582.0
8	4792.0
9	5214.5
10	5637.0
11	5805.5
12	5974.0
13	6063.5
14	6153.0
15	5807.5
16	5462.0
17	5279.0
18	5096.0
19	4847.0
20	4598.0
21	4551.5
22	4505.0
23	4660.5
24	4816.0
25	6390.5
26	9870.0
27	11775.0
28	12368.5
29	12962.0
30	15616.5
31	18271.0
32	20889.0
33	23507.0
34	27974.5
35	32442.0
36	35305.0
37	38168.0
38	45608.5
39	53049.0
40	69743.5
41	86438.0
42	98586.0
43	110734.0
44	116598.0
45	122462.0
46	112908.5
47	103355.0
48	98447.0
49	93539.0
50	92448.0
51	91357.0
52	84825.5
53	78294.0
54	72558.0
55	66822.0
56	64735.0
57	62648.0
58	57752.5
59	52857.0
60	48482.5
61	44108.0
62	39017.5
63	33927.0
64	31174.5
65	28422.0
66	25054.0
67	21686.0
68	18392.5
69	15099.0
70	12828.5
71	10558.0
72	9690.5
73	8823.0
74	7077.5
75	3939.5
76	2547.0
77	1992.5
78	1438.0
79	1056.0
80	674.0
81	637.5
82	601.0
83	393.0
84	185.0
85	185.0
86	185.0
87	105.5
88	26.0
89	16.0
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1302565.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.6659536209311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62263719710356	14.862324530973584
2	7.3309862427848	2.7367969840701
3	2.6942108367193565	1.508700435696404
4	1.4650941961542268	1.0938952126284052
5	0.9020390169344752	0.8418709227184598
6	0.6010790202679442	0.6731827868901691
7	0.4467491520908062	0.5837299267182098
8	0.3772630159404043	0.5633579166748941
9	0.31596065262707373	0.5307936199178471
>10	3.7012922375882815	16.163567646696325
>50	1.1727872041687395	15.77813019735028
>100	1.3637156411019087	43.51112938662736
>500	0.005360841649327128	0.6792889081386552
>1k	8.247448691272505E-4	0.47323152489931475
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4411	0.3386395304648904	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1737	0.1333522703281602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04237792355851723	0.0
2	0.0	0.0	0.0	0.1727361014613474	0.0
3	0.0	0.0	0.0	0.23438369678288606	0.0
4	7.677160064948774E-5	0.0	0.0	0.3461631473285402	0.0
5	7.677160064948774E-5	0.0	0.0	0.5981275406601589	0.0
6	7.677160064948774E-5	0.0	0.0	0.8511667364008706	0.0
7	7.677160064948774E-5	0.0	0.0	1.0258988994791047	0.0
8	7.677160064948774E-5	0.0	0.0	1.4615777331649475	0.0
9	7.677160064948774E-5	0.0	0.0	1.6234122673340678	0.0
10	7.677160064948774E-5	0.0	0.0	1.9073136465358735	0.0
11	7.677160064948774E-5	0.0	0.0	2.1150575978933874	0.0
12	7.677160064948774E-5	0.0	0.0	2.3250279256697364	0.0
13	7.677160064948774E-5	0.0	0.0	2.3988822054945436	0.0
14	7.677160064948774E-5	0.0	0.0	2.4302050185595343	0.0
15	7.677160064948774E-5	0.0	0.0	2.479108528173258	0.0
16	7.677160064948774E-5	0.0	0.0	2.56724232571887	0.0
17	7.677160064948774E-5	0.0	0.0	2.66320682653073	0.0
18	7.677160064948774E-5	0.0	0.0	2.8136791638037257	0.0
19	7.677160064948774E-5	0.0	0.0	2.879779511962935	0.0
20	7.677160064948774E-5	0.0	0.0	2.9466475761286386	0.0
21	7.677160064948774E-5	0.0	0.0	3.0390038117099722	0.0
22	7.677160064948774E-5	0.0	0.0	3.1327419361029967	0.0
23	7.677160064948774E-5	0.0	0.0	3.239991862210331	0.0
24	7.677160064948774E-5	0.0	0.0	3.3236729069182727	0.0
25	7.677160064948774E-5	0.0	0.0	3.3897732550774817	0.0
26	7.677160064948774E-5	0.0	0.0	3.4501925047886286	0.0
27	7.677160064948774E-5	0.0	0.0	3.516216081347188	0.0
28	7.677160064948774E-5	0.0	0.0	3.5825467443083454	0.0
29	7.677160064948774E-5	0.0	0.0	3.658704172152637	0.0
30	7.677160064948774E-5	0.0	0.0	3.756127333376837	0.0
31	7.677160064948774E-5	0.0	0.0	3.838349717672439	0.0
32	2.3031480194846322E-4	0.0	0.0	3.916503207133617	0.0
33	2.3031480194846322E-4	0.0	0.0	3.989820085753878	0.0
34	2.3031480194846322E-4	0.0	0.0	4.071735383646882	0.0
35	2.3031480194846322E-4	0.0	0.0	4.176989248137329	0.0
36	2.3031480194846322E-4	0.0	0.0	4.258443916426436	0.0
37	2.3031480194846322E-4	0.0	0.0	4.345579683163604	0.0
38	2.3031480194846322E-4	0.0	0.0	4.432945764702721	0.0
39	2.3031480194846322E-4	0.0	0.0	4.521693735053529	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTATT	20	7.033633E-4	45.000004	43
CGAGTTA	30	2.1657252E-6	45.000004	38
TACGGTA	30	2.1657252E-6	45.000004	6
CGACAGT	20	7.033633E-4	45.000004	41
CGCCTAG	20	7.033633E-4	45.000004	37
CGCTAAG	40	6.8157533E-9	45.000004	15
ACTCGCT	30	2.1657252E-6	45.000004	10
CGACTAG	20	7.033633E-4	45.000004	16
TGCGTTA	20	7.033633E-4	45.000004	35
ACGTTAG	100	0.0	45.0	1
ATTAACG	25	3.8910937E-5	45.0	1
CTAAGCG	50	2.1827873E-11	45.0	1
ACAATCG	25	3.8910937E-5	45.0	18
GGACGTA	35	1.2121018E-7	45.0	8
CGTACTG	35	1.2121018E-7	45.0	11
CTCGACC	25	3.8910937E-5	45.0	36
ACGTAAG	35	1.2121018E-7	45.0	1
CAATCGA	45	3.8562575E-10	45.0	41
TACGTAG	95	0.0	45.0	1
CTTACGA	35	1.2121018E-7	45.0	44
>>END_MODULE