##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548955_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1903640
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.865524994221598	31.0	31.0	33.0	30.0	33.0
2	31.181618898531234	31.0	31.0	33.0	30.0	34.0
3	31.33947910319178	31.0	31.0	34.0	28.0	34.0
4	35.18661196444706	37.0	35.0	37.0	32.0	37.0
5	35.049924880754766	37.0	35.0	37.0	32.0	37.0
6	35.11846304973629	37.0	35.0	37.0	32.0	37.0
7	35.545452921770924	36.0	35.0	37.0	35.0	37.0
8	35.630849320249624	37.0	35.0	37.0	35.0	37.0
9	37.47438696392175	39.0	37.0	39.0	35.0	39.0
10	36.94544083965456	39.0	37.0	39.0	32.0	39.0
11	36.517155554621674	39.0	35.0	39.0	32.0	39.0
12	34.94360908575151	35.0	34.0	39.0	30.0	39.0
13	34.420570065768736	35.0	33.0	39.0	27.0	39.0
14	35.01010957954235	35.0	33.0	40.0	27.0	41.0
15	35.50110420037402	35.0	33.0	40.0	30.0	41.0
16	35.7329516084974	35.0	34.0	40.0	31.0	41.0
17	35.58400485385892	35.0	34.0	40.0	31.0	41.0
18	35.691080771574455	36.0	35.0	39.0	31.0	41.0
19	35.53367548486058	36.0	34.0	39.0	30.0	41.0
20	35.245997667626234	35.0	34.0	39.0	30.0	41.0
21	35.092143472505306	35.0	34.0	39.0	30.0	41.0
22	34.91139658759009	35.0	34.0	39.0	29.0	41.0
23	34.87376867474943	35.0	34.0	39.0	29.0	41.0
24	34.98378790107373	35.0	34.0	39.0	30.0	41.0
25	34.89831007963691	35.0	34.0	39.0	30.0	41.0
26	34.62327698514425	35.0	34.0	38.0	29.0	41.0
27	34.61990134689332	35.0	34.0	39.0	29.0	41.0
28	34.75183595637831	35.0	34.0	39.0	29.0	41.0
29	34.76117700825787	35.0	34.0	39.0	29.0	41.0
30	34.713451072681806	35.0	34.0	38.0	29.0	41.0
31	34.40819430144355	35.0	34.0	38.0	28.0	41.0
32	34.25728446555021	35.0	34.0	39.0	27.0	41.0
33	34.01005232081696	35.0	33.0	39.0	26.0	41.0
34	33.80679487718266	35.0	33.0	39.0	24.0	41.0
35	33.547297283099745	35.0	33.0	38.0	23.0	41.0
36	33.26406095690361	35.0	33.0	38.0	23.0	41.0
37	33.270441364963965	35.0	32.0	38.0	23.0	41.0
38	33.3491510999979	35.0	32.0	38.0	23.0	41.0
39	33.36443550251098	35.0	33.0	38.0	23.0	41.0
40	32.958983841482635	35.0	32.0	38.0	23.0	41.0
41	33.1089234309008	35.0	32.0	38.0	23.0	41.0
42	33.117586833645014	35.0	32.0	38.0	23.0	41.0
43	33.07504675253725	35.0	32.0	38.0	23.0	41.0
44	33.03743197243176	35.0	32.0	38.0	23.0	41.0
45	33.11383087138324	35.0	32.0	38.0	23.0	40.0
46	33.14249910697401	35.0	33.0	38.0	23.0	40.0
47	33.07797062469795	35.0	32.0	38.0	23.0	40.0
48	33.12475573112563	35.0	33.0	38.0	23.0	40.0
49	33.16769557269232	35.0	32.0	38.0	23.0	40.0
50	32.91499443172028	35.0	32.0	38.0	23.0	40.0
51	32.60048380996407	35.0	31.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	4.0
14	13.0
15	44.0
16	147.0
17	477.0
18	1220.0
19	2588.0
20	4483.0
21	7411.0
22	11264.0
23	16190.0
24	22807.0
25	31008.0
26	39317.0
27	44703.0
28	49030.0
29	55765.0
30	66376.0
31	81479.0
32	103830.0
33	137980.0
34	256947.0
35	374146.0
36	97294.0
37	115877.0
38	162684.0
39	220434.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.605335042339938	26.41003551091593	30.410581832699464	13.574047614044673
2	29.680349225693938	25.847271542938792	32.143630098127794	12.328749133239478
3	26.727479985711582	25.660418986783217	34.628291063436365	12.983809964068838
4	25.652224160030258	28.297524742073083	32.07140005463218	13.978851043264484
5	21.193870689836313	32.706446597045655	32.72451724065474	13.37516547246328
6	19.224853438675378	41.88838225714946	29.498644701729322	9.38811960244584
7	87.39835262969889	4.213506755478977	7.158759009056334	1.229381605765796
8	89.46166291945956	2.7755773150385576	6.557752516232061	1.2050072492698198
9	85.89827908638188	4.1883969658128635	8.186631926204536	1.7266920216007227
10	63.18326994599819	17.889727049232	12.522693366392806	6.404309638377004
11	60.153600470677226	14.922516862431973	17.774158979639008	7.149723687251791
12	54.50620915719359	19.898615284402513	18.585762013826145	7.009413544577757
13	16.96906978210166	56.59195015864344	18.263799878128218	8.175180181126683
14	9.643472505305626	56.58722237397827	24.779107394255217	8.990197726460886
15	8.111932928494884	22.261509529112647	61.30602424828224	8.32053329411023
16	8.436258956525393	14.793710995776513	60.02710596541363	16.742924082284468
17	9.062585362778677	15.311245823790212	31.00565232922191	44.6205164842092
18	15.534975100334098	21.85612825954487	46.742241180055046	15.866655460065978
19	33.384463448971445	17.98128847891408	28.559023764997583	20.075224307116894
20	33.17549536677103	19.63144291987981	30.296852346031812	16.89620936731735
21	15.261919270450294	33.9330965938938	33.69518396335442	17.109800172301483
22	19.84161921371688	23.473240738795152	20.972557836565738	35.712582210922236
23	11.335809291672795	34.80048748712992	25.007511924523545	28.85619129667374
24	9.58132840242903	19.103717089365638	58.91581391439558	12.399140593809754
25	9.229738816162719	23.252453194931817	51.21131096215671	16.306497026748758
26	8.689615683637662	41.650627219432245	30.41047677081801	19.249280326112082
27	8.510800361412873	53.18143136307285	26.80328213317644	11.504486142337838
28	7.663844004118426	36.41092853690824	44.44942321027085	11.475804248702486
29	8.423914185455233	24.881332604904287	42.29691538316068	24.397837826479794
30	13.323369964909332	44.070359942005844	29.45031623626316	13.155953856821668
31	32.69919732722574	30.406274295560088	26.83963354415751	10.05489483305667
32	35.8638713202076	20.73648378895169	32.43465150973924	10.964993381101468
33	36.87672038830871	27.10512491857704	21.287901073732428	14.730253619381816
34	19.129667374083333	26.890010716311906	24.729255531508056	29.251066378096695
35	18.289855224727365	23.635141098106786	36.090227143787686	21.98477653337816
36	40.821741505746886	24.34556954045933	22.625391355508395	12.20729759828539
37	15.117669307221954	45.07926918955265	29.311844676514465	10.491216826710932
38	16.356611544199534	43.079994116534635	21.93723603202286	18.626158307242964
39	19.84330020382005	39.902765228719716	27.560725767477045	12.693208799983191
40	28.158843058561494	22.688796200962365	25.275419722216384	23.876941018259757
41	12.105650227984283	23.31601563320796	28.966768926897945	35.61156521190981
42	16.22496900674497	19.30223151436196	27.854111071421066	36.618688407472
43	16.573984576915805	19.98392553213843	31.529438339181777	31.912651551763986
44	11.013479439389801	30.54206677733185	36.37436700216428	22.070086781114075
45	10.227143787690949	55.60189951881658	20.701340589607277	13.469616103885187
46	19.098463995293226	42.14620411422328	24.153726544934965	14.601605345548526
47	17.556365699396945	29.84713496249291	25.37911579920573	27.217383538904414
48	18.28145027421151	27.289771175222207	39.7843079573869	14.644470593179381
49	23.86391334496018	25.591761047256835	38.30902901809166	12.235296589691329
50	20.08998550146036	44.90849110125864	22.52773633670232	12.473787060578681
51	16.0322329852283	39.36595154546028	23.52992162383644	21.071893845474985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4210.0
1	4181.0
2	4152.0
3	8921.0
4	13690.0
5	11162.5
6	8635.0
7	9473.0
8	10311.0
9	11039.5
10	11768.0
11	11917.0
12	12066.0
13	11869.5
14	11673.0
15	10942.5
16	10212.0
17	9478.5
18	8745.0
19	8668.0
20	8591.0
21	8120.5
22	7650.0
23	8970.5
24	10291.0
25	11274.0
26	15659.0
27	19061.0
28	24753.5
29	30446.0
30	34820.5
31	39195.0
32	48360.5
33	57526.0
34	65628.0
35	73730.0
36	72565.0
37	71400.0
38	90793.0
39	110186.0
40	160304.0
41	210422.0
42	261238.5
43	312055.0
44	298229.0
45	284403.0
46	243346.0
47	202289.0
48	173064.5
49	143840.0
50	119280.5
51	94721.0
52	71502.0
53	48283.0
54	35444.5
55	22606.0
56	17931.0
57	13256.0
58	11527.5
59	9799.0
60	8889.5
61	7980.0
62	6889.5
63	5799.0
64	5003.0
65	4207.0
66	3389.0
67	2571.0
68	2212.5
69	1854.0
70	1666.0
71	1478.0
72	1253.0
73	1028.0
74	841.0
75	463.5
76	273.0
77	220.5
78	168.0
79	135.5
80	103.0
81	61.5
82	20.0
83	17.5
84	15.0
85	14.0
86	13.0
87	9.0
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1903640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.940363866685306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.96200044233437	13.71525746867881
2	7.703900762769342	2.6101376422829423
3	2.7645276330945108	1.4049631207238193
4	1.3879586241420154	0.940500964994786
5	0.9192506153512451	0.7786219954362233
6	0.6012537074971361	0.6111273948717036
7	0.468400935312829	0.5554417595786535
8	0.3816075658491729	0.517165681581205
9	0.3031294311355117	0.4621610575923205
>10	2.826731761641774	10.264198152302734
>50	0.5001389517375672	6.054567702352053
>100	1.0656350262757437	48.257858406522786
>500	0.1048828866398682	11.087684719040295
>1k	0.010270431035951483	2.3699371180758164
>5k	3.112251829076207E-4	0.37037681596588756
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7025	0.36902985858670756	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3385	0.17781723435103275	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2434	0.1278603097224265	No Hit
TTTGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2032	0.10674287155134374	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02542497531045786	0.0
2	0.0	0.0	0.0	0.12276480847219012	0.0
3	0.0	0.0	0.0	0.18291273560126914	0.0
4	0.0	0.0	0.0	0.2931751801811267	0.0
5	0.0	0.0	0.0	0.5196360656426635	0.0
6	0.0	0.0	0.0	0.7312832258200079	0.0
7	0.0	0.0	0.0	0.8621903301044315	0.0
8	5.2530940724086486E-5	0.0	0.0	1.1572566241516253	0.0
9	5.2530940724086486E-5	0.0	0.0	1.2735075959740287	0.0
10	5.2530940724086486E-5	0.0	0.0	1.4719694900296274	0.0
11	5.2530940724086486E-5	0.0	0.0	1.6864007900653486	0.0
12	5.2530940724086486E-5	0.0	0.0	1.8626420961946586	0.0
13	5.2530940724086486E-5	0.0	0.0	1.9336639280536236	0.0
14	5.2530940724086486E-5	0.0	0.0	1.9597192746527705	0.0
15	5.2530940724086486E-5	0.0	0.0	2.0034775482759346	0.0
16	5.2530940724086486E-5	0.0	0.0	2.116314008951272	0.0
17	5.2530940724086486E-5	0.0	0.0	2.241862957281839	0.0
18	5.2530940724086486E-5	0.0	0.0	2.441007753566851	0.0
19	5.2530940724086486E-5	0.0	0.0	2.5169149629131558	0.0
20	5.2530940724086486E-5	0.0	0.0	2.5956588430585614	0.0
21	5.2530940724086486E-5	0.0	0.0	2.7120674077031373	0.0
22	5.2530940724086486E-5	0.0	0.0	2.8245886827341304	0.0
23	5.2530940724086486E-5	0.0	0.0	2.9660545061040953	0.0
24	5.2530940724086486E-5	0.0	0.0	3.066808850412893	0.0
25	5.2530940724086486E-5	0.0	0.0	3.148021684772331	0.0
26	5.2530940724086486E-5	0.0	0.0	3.2391103359878968	0.0
27	5.2530940724086486E-5	0.0	0.0	3.3234224958500556	0.0
28	5.2530940724086486E-5	0.0	0.0	3.4115168834443486	0.0
29	5.2530940724086486E-5	0.0	0.0	3.513111722804732	0.0
30	5.2530940724086486E-5	0.0	0.0	3.6589376142547962	0.0
31	5.2530940724086486E-5	0.0	0.0	3.8019793658464835	0.0
32	5.2530940724086486E-5	0.0	0.0	3.9189132398983	0.0
33	5.2530940724086486E-5	0.0	0.0	4.032064886217983	0.0
34	5.2530940724086486E-5	0.0	0.0	4.158244205837238	0.0
35	1.0506188144817297E-4	0.0	0.0	4.337532306528545	0.0
36	1.0506188144817297E-4	0.0	0.0	4.470645710323381	0.0
37	1.0506188144817297E-4	0.0	0.0	4.613214683448551	0.0
38	1.0506188144817297E-4	0.0	0.0	4.739446534008531	0.0
39	1.0506188144817297E-4	0.0	0.0	4.866729003382993	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	20	7.034454E-4	45.000004	6
GTACCAC	20	7.034454E-4	45.000004	9
CGAACAC	20	7.034454E-4	45.000004	23
GCGACGG	20	7.034454E-4	45.000004	2
GTCGATG	20	7.034454E-4	45.000004	1
GGTCGTA	20	7.034454E-4	45.000004	8
GATCGCT	40	6.8193913E-9	45.000004	9
ACGTTCG	20	7.034454E-4	45.000004	2
ACGTTAT	20	7.034454E-4	45.000004	40
GCGAACA	20	7.034454E-4	45.000004	22
ACACCCG	20	7.034454E-4	45.000004	1
CATCTCG	40	6.8193913E-9	45.000004	20
TAACGCA	40	6.8193913E-9	45.000004	28
CCTCGGG	40	6.8193913E-9	45.000004	3
TCTAGCG	20	7.034454E-4	45.000004	1
GTAATCG	20	7.034454E-4	45.000004	1
CCCGTCG	20	7.034454E-4	45.000004	37
CGCTAAT	40	6.8193913E-9	45.000004	25
GTCGTAA	20	7.034454E-4	45.000004	38
CTCGCTA	40	6.8193913E-9	45.000004	23
>>END_MODULE