##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548954_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2034344
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87514009430067	31.0	31.0	33.0	30.0	33.0
2	31.193248536137446	31.0	31.0	33.0	30.0	34.0
3	31.346501378331293	31.0	31.0	34.0	28.0	34.0
4	35.18743880091076	37.0	35.0	37.0	33.0	37.0
5	35.03996226793502	37.0	35.0	37.0	32.0	37.0
6	35.11380621959708	37.0	35.0	37.0	32.0	37.0
7	35.560284298034155	36.0	35.0	37.0	35.0	37.0
8	35.6429753276732	37.0	35.0	37.0	35.0	37.0
9	37.49680191747315	39.0	37.0	39.0	35.0	39.0
10	37.03655035726504	39.0	37.0	39.0	33.0	39.0
11	36.52381357331897	39.0	35.0	39.0	32.0	39.0
12	34.656209569276385	35.0	33.0	39.0	30.0	39.0
13	34.04582312529248	35.0	33.0	39.0	25.0	39.0
14	34.57790521170461	35.0	33.0	39.0	25.0	41.0
15	35.16445842001156	35.0	33.0	39.0	30.0	41.0
16	35.47460852245245	35.0	34.0	39.0	31.0	41.0
17	35.35388115284337	35.0	34.0	39.0	31.0	41.0
18	35.485822456772304	36.0	34.0	39.0	31.0	41.0
19	35.3168539833971	36.0	34.0	38.0	30.0	41.0
20	35.00511122995914	35.0	34.0	38.0	30.0	41.0
21	34.790992575493625	35.0	34.0	38.0	29.0	41.0
22	34.60703302882895	35.0	33.0	38.0	29.0	41.0
23	34.599541178876336	35.0	34.0	38.0	29.0	41.0
24	34.71993969554805	35.0	34.0	38.0	29.0	41.0
25	34.65294807564502	35.0	34.0	38.0	29.0	41.0
26	34.39827335003323	35.0	34.0	38.0	29.0	40.0
27	34.392646966294784	35.0	34.0	38.0	29.0	40.0
28	34.534358004349315	35.0	34.0	38.0	29.0	40.0
29	34.583870279559406	35.0	34.0	38.0	29.0	40.0
30	34.540294070226075	35.0	34.0	38.0	29.0	40.0
31	34.18993493725741	35.0	33.0	38.0	29.0	40.0
32	34.01101288670942	35.0	34.0	38.0	27.0	41.0
33	33.753118449976995	35.0	33.0	38.0	27.0	41.0
34	33.578803781464686	35.0	33.0	38.0	24.0	41.0
35	33.31030297727425	35.0	33.0	38.0	23.0	41.0
36	33.00029591848773	35.0	33.0	38.0	23.0	40.0
37	32.998589225814314	35.0	32.0	38.0	23.0	40.0
38	33.115009555905985	35.0	32.0	38.0	23.0	40.0
39	33.16744365751318	35.0	33.0	38.0	23.0	40.0
40	32.718096349486615	35.0	32.0	38.0	23.0	40.0
41	32.91742055424255	35.0	32.0	37.0	23.0	40.0
42	32.89025749824022	35.0	32.0	38.0	23.0	40.0
43	32.84685087674454	35.0	32.0	37.0	23.0	40.0
44	32.797017613540284	35.0	32.0	37.0	23.0	40.0
45	32.87986348424848	35.0	32.0	37.0	23.0	40.0
46	32.89924368739997	35.0	32.0	37.0	23.0	40.0
47	32.869373124702605	35.0	32.0	37.0	23.0	40.0
48	32.90516402339034	35.0	32.0	37.0	23.0	40.0
49	32.96970423881113	35.0	32.0	37.0	23.0	40.0
50	32.64593058007888	35.0	32.0	37.0	23.0	40.0
51	32.39357109712025	35.0	31.0	36.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	1.0
13	5.0
14	11.0
15	39.0
16	204.0
17	560.0
18	1383.0
19	2815.0
20	4973.0
21	8067.0
22	12107.0
23	17679.0
24	24987.0
25	33605.0
26	42338.0
27	49140.0
28	54354.0
29	61307.0
30	72940.0
31	89093.0
32	114634.0
33	154881.0
34	298034.0
35	441079.0
36	92314.0
37	105207.0
38	145910.0
39	206500.0
40	174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.72016728734177	25.421167708116226	32.50217269055774	13.356492313984262
2	29.390162135803976	25.811170578820498	32.96128874959201	11.837378535783525
3	26.238777709177995	26.007695846916747	34.8826943722399	12.870832071665362
4	24.858381866586967	28.641272075912433	33.2605498381788	13.239796219321805
5	21.698542626025883	33.546686302808176	32.24548060701631	12.509290464149622
6	19.498423078889314	41.6575564407986	28.91742989386259	9.926590586449489
7	87.44814053080502	4.953046289123177	6.517973361437397	1.08083981863441
8	90.68559692952618	2.666805613996453	5.539279492553865	1.1083179639235057
9	87.68207343497461	3.80722237733638	7.110891766584215	1.3998124211047887
10	70.32458620567613	15.422662047323362	9.330329580444605	4.9224221665559025
11	67.90493643159662	12.483729398764417	14.438020315148274	5.1733138544906865
12	59.090055565823675	19.73668170181641	15.25273011840672	5.9205326139531955
13	16.326393176375284	61.7786863971875	15.988937957395603	5.905982469041617
14	7.910903957246168	60.86792597515465	24.781403735061524	6.439766332537664
15	6.138096605097269	20.040661756320468	67.12724101725175	6.694000621330512
16	7.01169517053163	13.406631326855242	64.36694089101942	15.214732611593712
17	7.529945771216667	13.471762887692543	30.030073576543593	48.9682177645472
18	14.245624142229632	20.612738061999348	49.313587082617296	15.828050713153724
19	35.179645133763024	15.999555630709459	28.698047134604572	20.12275210092295
20	35.69656852528383	18.185026721144506	29.617606461837333	16.500798291734338
21	16.174943863967943	34.399737704144435	32.77877291156265	16.646545520324977
22	20.77691875120432	23.42543837227136	18.14226109251926	37.655381784005066
23	9.678304160948198	35.53071653564982	24.019339895317607	30.771639408084372
24	8.65881089923828	18.581960573039762	61.736461483406934	11.022767044315023
25	7.330520305317095	22.026707380855942	54.40751416672893	16.235258147098033
26	6.9203635176744935	44.79979787095988	29.49717451915704	18.782664092208595
27	7.322262114961875	57.90087615467198	25.584217811736853	9.192643918629297
28	6.600407797304684	37.25250006881825	45.48970085688556	10.6573912769915
29	6.453529983129696	25.005456304341845	43.60747248253	24.933541229998465
30	12.517991057559586	47.41287609175242	28.4721757972103	11.59695705347768
31	35.103895899611864	30.95582654654277	25.168801343332298	8.77147621051307
32	39.85437074555729	19.819951787898212	32.07087886807737	8.254798598467122
33	39.97396703802307	28.369784067984572	18.33908129598534	13.317167598007023
34	20.139121013948476	26.35989783438789	22.80086357076286	30.70011758090077
35	17.689486143936325	22.609794607008453	36.503020138187054	23.19769911086817
36	44.75088775546319	22.970451408414704	22.320807100470716	9.957853735651394
37	13.849525940548896	47.075125937402916	28.802552567314084	10.272795554734106
38	15.393856692870036	45.91848772872238	19.1767469021955	19.510908676212086
39	19.4875596261006	41.04551639250785	27.10908282964926	12.357841151742281
40	29.570220179084757	21.44740515861624	23.54542791189691	25.436946750402097
41	11.892580605836574	22.084416401552538	28.227330284357024	37.79567270825386
42	15.959591888097588	17.408461892383983	27.513340909895277	39.11860530962315
43	16.710595651472907	17.05665315207261	30.486535217249394	35.74621597920509
44	9.775436209411978	30.460433437019503	36.97127919368602	22.7928511598825
45	10.344120758337823	57.98026292505103	20.12530820746147	11.550308109149682
46	20.546377603787757	43.266576350902305	22.321052879945576	13.865993165364362
47	16.738221264446917	31.55955924858333	23.43925117875836	28.262968308211395
48	18.768064791402043	26.9956801799499	40.76237843747174	13.473876591176321
49	25.862636800855704	23.509740732147563	39.49459875026053	11.133023716736206
50	19.98806494870091	46.56700145108202	21.258941457295325	12.185992142921748
51	15.048290751220051	42.8020039875262	21.680109165411555	20.4695960958422
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3588.0
1	3770.0
2	3952.0
3	7823.0
4	11694.0
5	9787.0
6	7880.0
7	8910.5
8	9941.0
9	11039.0
10	12137.0
11	12380.5
12	12624.0
13	12384.5
14	12145.0
15	11364.5
16	10584.0
17	9774.5
18	8965.0
19	9086.0
20	9207.0
21	8800.0
22	8393.0
23	10099.0
24	11805.0
25	12920.5
26	17199.5
27	20363.0
28	21656.0
29	22949.0
30	28901.5
31	34854.0
32	46177.0
33	57500.0
34	57731.0
35	57962.0
36	63555.5
37	69149.0
38	92300.0
39	115451.0
40	177829.5
41	240208.0
42	299365.0
43	358522.0
44	348515.0
45	338508.0
46	286494.5
47	234481.0
48	193193.5
49	151906.0
50	124826.0
51	97746.0
52	71989.0
53	46232.0
54	32575.5
55	18919.0
56	14416.5
57	9914.0
58	8503.0
59	7092.0
60	6074.5
61	5057.0
62	4345.0
63	3633.0
64	2943.0
65	2253.0
66	1941.5
67	1630.0
68	1292.0
69	954.0
70	891.5
71	829.0
72	700.0
73	571.0
74	488.5
75	282.5
76	159.0
77	117.5
78	76.0
79	53.0
80	30.0
81	24.0
82	18.0
83	16.5
84	15.0
85	9.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2034344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.57751660431873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.29726661716327	11.851122539974245
2	8.307151049669793	2.4219526472229034
3	2.800268458255279	1.2246287984029902
4	1.3757783924484124	0.8022172943911865
5	0.7810792771234978	0.5693098065778529
6	0.5478228296299784	0.47915378370935285
7	0.42003472568306677	0.4286144231624758
8	0.3063693655633448	0.3572883610843403
9	0.2621425247023802	0.3439248305892282
>10	2.321182496953999	7.336916573231744
>50	0.448013115479596	4.5158539791252865
>100	0.7407104902925977	27.856685747592756
>500	0.35935794458233816	35.0242208114897
>1k	0.03248433397354469	6.497425337918387
>5k	3.3837847889109054E-4	0.2906850655275397
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5893	0.2896756890673357	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3328	0.16359081846531362	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3131	0.15390710715591857	No Hit
GATAGCGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCA	2364	0.11620453571274082	No Hit
TATACAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2178	0.10706153924803277	No Hit
TTTTGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2126	0.10450543270951226	No Hit
TTGTTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2105	0.10347315891510973	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.037260168388433816	0.0
2	0.0	0.0	0.0	0.14427255174149506	0.0
3	0.0	0.0	0.0	0.19682020346608048	0.0
4	0.0	0.0	0.0	0.3024070658649668	0.0
5	0.0	0.0	0.0	0.49293531477468905	0.0
6	0.0	0.0	0.0	0.6907386361402005	0.0
7	0.0	0.0	0.0	0.8162336360025639	0.0
8	0.0	0.0	0.0	1.1517717750783545	0.0
9	0.0	0.0	0.0	1.2839519766568486	0.0
10	0.0	0.0	0.0	1.4999921350568046	0.0
11	0.0	0.0	0.0	1.6930764905050473	0.0
12	0.0	0.0	0.0	1.8689562827132482	0.0
13	0.0	0.0	0.0	1.9398390832622212	0.0
14	0.0	0.0	0.0	1.9625491067390766	0.0
15	0.0	0.0	0.0	2.0021196021911734	0.0
16	0.0	0.0	0.0	2.100578859819185	0.0
17	0.0	0.0	0.0	2.2146696920481492	0.0
18	0.0	0.0	0.0	2.4374933639541787	0.0
19	0.0	0.0	0.0	2.5037555103758264	0.0
20	0.0	0.0	0.0	2.5781775353627507	0.0
21	0.0	0.0	0.0	2.677374131415336	0.0
22	0.0	0.0	0.0	2.7644292214099484	0.0
23	0.0	0.0	0.0	2.8750791409909042	0.0
24	0.0	0.0	0.0	2.95176233714652	0.0
25	0.0	0.0	0.0	3.0142886355503298	0.0
26	0.0	0.0	0.0	3.089300531276913	0.0
27	0.0	0.0	0.0	3.1490249436673445	0.0
28	0.0	0.0	0.0	3.214746375244305	0.0
29	0.0	0.0	0.0	3.294428080993185	0.0
30	0.0	0.0	0.0	3.425723476462191	0.0
31	0.0	0.0	0.0	3.555445883292108	0.0
32	0.0	0.0	0.0	3.6471707833090177	0.0
33	0.0	0.0	0.0	3.737273538791866	0.0
34	0.0	0.0	0.0	3.8292442182836335	0.0
35	0.0	0.0	0.0	3.989246656416024	0.0
36	0.0	0.0	0.0	4.100191511366809	0.0
37	0.0	0.0	0.0	4.219738647937615	0.0
38	0.0	0.0	0.0	4.318738620410314	0.0
39	0.0	0.0	0.0	4.421621908585765	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	115	0.0	45.000004	1
CCTTAAG	20	7.0345675E-4	45.000004	33
GGTACCG	115	0.0	45.000004	1
GCGACAG	45	3.8562575E-10	45.000004	1
GTCGATC	20	7.0345675E-4	45.000004	1
CGTATAA	35	1.2125383E-7	45.000004	26
TTAGCGA	80	0.0	45.000004	1
CGTGATG	40	6.8193913E-9	45.000004	42
CGTGAGA	20	7.0345675E-4	45.000004	20
CTATACG	35	1.2125383E-7	45.000004	1
CGCATGA	90	0.0	45.000004	41
GACAACG	35	1.2125383E-7	45.000004	41
GATCCGG	40	6.8193913E-9	45.000004	2
TTCACGC	20	7.0345675E-4	45.000004	19
ATTCGTG	25	3.891868E-5	45.000004	1
TTCGTGA	80	0.0	45.000004	1
CGTCTGG	80	0.0	45.000004	2
AAGCGTG	20	7.0345675E-4	45.000004	34
ACCCTTG	25	3.891868E-5	45.000004	25
CGACAAG	35	1.2125383E-7	45.000004	24
>>END_MODULE