FastQCFastQC Report
Sat 18 Jun 2016
SRR3548952_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548952_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2191290
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92500.42212577979181215No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT24480.11171501718166012No Hit
GTAATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA23480.10715149523796484No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC21930.1000780362252372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG551.8189894E-1245.0000042
TACCCGA600.045.00000422
TAGGTAC207.034687E-445.00000414
ACTCTCG406.8193913E-945.0000041
TAGCCGT207.034687E-445.00000444
CGTTACA207.034687E-445.00000420
ATATCGC302.1664073E-645.00000422
CGAGGTG600.045.0000044
TACGACT406.8193913E-945.00000417
TATTCGA406.8193913E-945.00000428
GACGGGT406.8193913E-945.0000044
GACGGAT207.034687E-445.00000439
GTATACG551.8189894E-1245.0000041
CGGTCTA1550.045.00000431
CCGCGTA207.034687E-445.00000412
ACGTAGC207.034687E-445.00000423
TAGGCCG551.8189894E-1245.0000041
CACACGG700.045.0000042
ACTCCGA351.2126111E-745.00000431
TATACGA406.8193913E-945.00000415