##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548952_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2191290
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.902912439704465	31.0	31.0	33.0	30.0	33.0
2	31.21494325260463	31.0	31.0	33.0	30.0	34.0
3	31.344883607372825	31.0	31.0	34.0	30.0	34.0
4	35.216386238243224	37.0	35.0	37.0	33.0	37.0
5	35.109021170178295	37.0	35.0	37.0	32.0	37.0
6	35.17468294931296	37.0	35.0	37.0	32.0	37.0
7	35.573840523162154	36.0	35.0	37.0	35.0	37.0
8	35.65421966056524	37.0	35.0	37.0	35.0	37.0
9	37.50034591496333	39.0	37.0	39.0	35.0	39.0
10	37.00348881252596	39.0	37.0	39.0	33.0	39.0
11	36.56652200302105	39.0	35.0	39.0	32.0	39.0
12	35.19018158253814	35.0	35.0	39.0	31.0	39.0
13	34.76315275477002	35.0	34.0	39.0	30.0	39.0
14	35.41298869615615	36.0	33.0	40.0	29.0	41.0
15	35.83225086592829	36.0	34.0	40.0	31.0	41.0
16	36.0338791305578	35.0	34.0	40.0	32.0	41.0
17	35.88504898940806	35.0	34.0	40.0	31.0	41.0
18	35.96492431398856	36.0	35.0	40.0	31.0	41.0
19	35.81180993843809	36.0	35.0	40.0	31.0	41.0
20	35.549425224411195	35.0	34.0	40.0	31.0	41.0
21	35.38959060644643	35.0	34.0	40.0	30.0	41.0
22	35.223817021024146	35.0	34.0	40.0	30.0	41.0
23	35.16646222088359	35.0	34.0	39.0	30.0	41.0
24	35.25864627685062	35.0	34.0	39.0	30.0	41.0
25	35.1555439946333	35.0	34.0	39.0	30.0	41.0
26	34.92771837593381	35.0	34.0	39.0	29.0	41.0
27	34.90463380018163	35.0	34.0	39.0	29.0	41.0
28	34.97836936233908	35.0	34.0	39.0	29.0	41.0
29	34.97347452870227	36.0	34.0	39.0	29.0	41.0
30	34.93340041710591	36.0	34.0	39.0	29.0	41.0
31	34.68724221805421	35.0	34.0	39.0	29.0	41.0
32	34.521738793130986	35.0	34.0	39.0	28.0	41.0
33	34.238342255018736	35.0	33.0	39.0	27.0	41.0
34	34.00467259011815	35.0	34.0	39.0	24.0	41.0
35	33.759720073563976	35.0	33.0	39.0	23.0	41.0
36	33.49441789995847	35.0	33.0	39.0	23.0	41.0
37	33.49834252883005	35.0	33.0	39.0	23.0	41.0
38	33.54356246777012	35.0	33.0	39.0	23.0	41.0
39	33.54723427752602	35.0	33.0	39.0	23.0	41.0
40	33.164490323051716	35.0	32.0	39.0	23.0	41.0
41	33.286535328505124	35.0	32.0	39.0	23.0	41.0
42	33.29985533635438	35.0	32.0	39.0	23.0	41.0
43	33.25293685454687	35.0	33.0	39.0	23.0	41.0
44	33.19452605542854	35.0	33.0	39.0	23.0	41.0
45	33.27364109725322	35.0	33.0	39.0	23.0	41.0
46	33.289535844183106	35.0	33.0	39.0	23.0	41.0
47	33.24331421217639	35.0	33.0	39.0	23.0	41.0
48	33.290621049701315	35.0	33.0	39.0	23.0	41.0
49	33.32651862601481	35.0	33.0	39.0	23.0	41.0
50	33.115115297381905	35.0	32.0	39.0	23.0	41.0
51	32.79780996581922	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	5.0
13	2.0
14	13.0
15	60.0
16	181.0
17	550.0
18	1319.0
19	2799.0
20	5114.0
21	8148.0
22	12529.0
23	18256.0
24	25661.0
25	35312.0
26	44683.0
27	50555.0
28	54106.0
29	60484.0
30	71426.0
31	88321.0
32	113283.0
33	151014.0
34	279758.0
35	403177.0
36	119645.0
37	145535.0
38	207523.0
39	291694.0
40	134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.931551734366515	24.88931177525567	29.155155182563696	15.023981307814118
2	29.02751347379854	27.133925678481624	32.35039634188081	11.488164505839027
3	26.62869816409512	26.66246822647847	35.18799428647053	11.520839322955885
4	24.63685774133045	28.851270256333027	34.27985341967516	12.232018582661356
5	22.24027855737944	32.01575327774964	33.25570782507108	12.488260339799844
6	19.051973951416745	41.202624937822016	30.43380839596767	9.31159271479357
7	86.58685066787145	4.477043202862241	7.79006886354613	1.1460372657201923
8	88.79221828238161	2.8386019194173295	7.293694581730397	1.0754852164706634
9	85.29003463713155	4.765001437509412	8.437404451259303	1.5075594740997311
10	59.45904010879436	22.16589314969721	12.727845241843847	5.647221499664582
11	55.253709002459736	15.915100237759495	20.86081714423924	7.97037361554153
12	50.57418233095574	20.574273601394612	20.811622377686202	8.039921689963446
13	17.344942933158094	53.57059083918605	19.517909541868033	9.566556685787823
14	9.871080505090609	52.683305267673376	26.072587380036417	11.373026847199595
15	8.050372155214507	23.05272236901551	58.77341657197359	10.123488903796392
16	8.683697730560537	17.407828265542218	56.34261097344486	17.565863030452384
17	9.865878090074796	16.99263903910482	31.42327122379968	41.7182116470207
18	15.63512816651379	23.50318762007767	45.353604497807225	15.508079715601312
19	31.083927732066503	20.042486389295803	29.559209415458476	19.314376463179222
20	31.546897033254385	20.282390737875865	30.975498450684302	17.195213778185455
21	15.14870236253531	33.84714026897399	32.58537208676168	18.41878528172903
22	19.112486252390145	24.183791282760385	22.550461143892413	34.153261320957064
23	11.38895353878309	34.29158167107047	26.86038817317653	27.459076616969913
24	10.19349333041268	20.192991342998873	56.9524800460003	12.661035280588145
25	9.307120463288749	26.62637076790384	47.888230220555016	16.1782785482524
26	9.488429190111761	41.109985442365	30.683706857604427	18.717878509918815
27	9.318392362489675	50.96851626211044	26.653386817810514	13.059704557589367
28	7.981417338645273	35.631659889836584	43.17538983886204	13.211532932656105
29	8.915433374861383	25.11242236308293	41.52517466880239	24.44696959325329
30	13.520163921708217	42.72036106585618	31.29106599309083	12.46840901934477
31	30.721584089737096	31.219007981599876	27.3004942294265	10.758913699236523
32	34.68294931296177	21.586554038945096	31.197650698903384	12.532845949189747
33	35.00841969798611	28.626334259728285	20.52101730031169	15.844228741973906
34	18.867927111427516	26.602594818577185	25.88730838912239	28.642169680872907
35	18.598816222407805	25.738309397660743	35.58753063264105	20.075343747290407
36	37.986437212783336	27.43758242861511	22.67070994710878	11.905270411492774
37	14.990941408941765	44.8556329833112	29.09053571184097	11.062889895906064
38	16.623267572982126	42.5742827284385	24.030183134135598	16.772266564443775
39	19.69492855806397	40.10696895436022	27.424348214978387	12.77375427259742
40	27.467929849540685	24.165217748449543	26.014585016132052	22.352267385877724
41	13.021279702823453	24.689612054999564	29.36398194670719	32.925126295469795
42	16.474131675862164	21.43522765129216	26.982964372584185	35.107676300261495
43	17.266039638751604	21.56579001410128	30.203487443469374	30.964682903677744
44	12.331594631472784	31.14548964308695	35.53062351400317	20.9922922114371
45	11.117332712694349	53.54129302830752	21.1297454923812	14.211628766616924
46	18.066298846798006	41.861369330394425	24.81743630464247	15.254895518165098
47	17.109921553057788	30.83193005033565	25.87234003714707	26.185808359459493
48	18.539672978017517	27.078935239060097	39.63564840801537	14.74574337490702
49	22.57683830072697	27.39349880663901	37.22505921169722	12.804603680936799
50	18.85428218081587	44.28313915547463	24.691026746802113	12.171551916907394
51	16.571425963701746	38.43868223740354	24.794299248387937	20.19559255050678
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2437.0
1	3535.0
2	4633.0
3	11724.0
4	18815.0
5	15470.5
6	12126.0
7	12774.5
8	13423.0
9	14254.5
10	15086.0
11	15198.0
12	15310.0
13	14737.0
14	14164.0
15	13482.5
16	12801.0
17	12053.5
18	11306.0
19	11141.5
20	10977.0
21	10937.0
22	10897.0
23	11654.5
24	12412.0
25	15385.0
26	21314.5
27	24271.0
28	32911.0
29	41551.0
30	51049.5
31	60548.0
32	70421.0
33	80294.0
34	88366.0
35	96438.0
36	95694.5
37	94951.0
38	121751.0
39	148551.0
40	193201.0
41	237851.0
42	280422.0
43	322993.0
44	313013.0
45	303033.0
46	257808.5
47	212584.0
48	180671.0
49	148758.0
50	128204.5
51	107651.0
52	77439.0
53	47227.0
54	36012.0
55	24797.0
56	20497.0
57	16197.0
58	14684.5
59	13172.0
60	11729.5
61	10287.0
62	8988.0
63	7689.0
64	6622.0
65	5555.0
66	4900.5
67	4246.0
68	3773.5
69	3301.0
70	3073.5
71	2846.0
72	2262.5
73	1679.0
74	1389.5
75	780.0
76	460.0
77	388.0
78	316.0
79	206.0
80	96.0
81	76.5
82	57.0
83	40.5
84	24.0
85	16.5
86	9.0
87	9.0
88	9.0
89	6.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2191290.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.839195701695722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.01884750678933	13.642922286926678
2	7.646233715118039	2.5751285181955317
3	2.766884916799839	1.3977634979418774
4	1.4357373073748143	0.9670664598044064
5	0.8989108680564991	0.7568468012792284
6	0.6435882266020625	0.6502504859435654
7	0.4869965154192445	0.5740440740431975
8	0.4015597088059912	0.5409554018000027
9	0.33548680281299437	0.5084395135113793
>10	2.8753965639785393	10.189885992196654
>50	0.4635768703868226	5.548080944533151
>100	0.7681802693411635	32.71150922794698
>500	0.2411792057516366	25.66384131129041
>1k	0.0171493114699245	3.8492622050757612
>5k	2.722112931734048E-4	0.4240032795111734
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9250	0.42212577979181215	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2448	0.11171501718166012	No Hit
GTAATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2348	0.10715149523796484	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2193	0.1000780362252372	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.019760050016200504	0.0
2	0.0	0.0	0.0	0.06744885432781604	0.0
3	0.0	0.0	0.0	0.09587959603703754	0.0
4	0.0	0.0	0.0	0.15096130589743942	0.0
5	0.0	0.0	0.0	0.24547184535136837	0.0
6	0.0	0.0	0.0	0.35750630906908715	0.0
7	0.0	0.0	0.0	0.4353143582090914	0.0
8	0.0	0.0	0.0	0.6361093237316832	0.0
9	0.0	0.0	0.0	0.732627812840838	0.0
10	0.0	0.0	0.0	0.8977816719831697	0.0
11	0.0	0.0	0.0	1.0316754058111888	0.0
12	0.0	0.0	0.0	1.1690374163164163	0.0
13	0.0	0.0	0.0	1.2230238809103313	0.0
14	0.0	0.0	0.0	1.243651000095834	0.0
15	0.0	0.0	0.0	1.273542068827038	0.0
16	0.0	0.0	0.0	1.3387547974024434	0.0
17	0.0	0.0	0.0	1.4221759785331929	0.0
18	0.0	0.0	0.0	1.5742325296971191	0.0
19	0.0	0.0	0.0	1.633877761501216	0.0
20	0.0	0.0	0.0	1.703517106362006	0.0
21	0.0	0.0	0.0	1.7907260107060226	0.0
22	0.0	0.0	0.0	1.8807186634356932	0.0
23	0.0	0.0	0.0	1.9901062844260686	0.0
24	0.0	0.0	0.0	2.0712457045849706	0.0
25	0.0	0.0	0.0	2.138238206718417	0.0
26	0.0	0.0	0.0	2.201899337832966	0.0
27	0.0	0.0	0.0	2.271447412254882	0.0
28	0.0	0.0	0.0	2.3422276376015954	0.0
29	0.0	0.0	0.0	2.430942504187031	0.0
30	0.0	0.0	0.0	2.5558917350054076	0.0
31	0.0	0.0	0.0	2.6615829032213902	0.0
32	0.0	0.0	0.0	2.757097417502932	0.0
33	0.0	0.0	0.0	2.848732938132333	0.0
34	0.0	0.0	0.0	2.9531006849846437	0.0
35	0.0	0.0	0.0	3.0931095382172145	0.0
36	0.0	0.0	0.0	3.211852379192165	0.0
37	0.0	0.0	0.0	3.327948377439773	0.0
38	0.0	0.0	0.0	3.4419907908127176	0.0
39	0.0	0.0	0.0	3.5586800469130058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGG	55	1.8189894E-12	45.000004	2
TACCCGA	60	0.0	45.000004	22
TAGGTAC	20	7.034687E-4	45.000004	14
ACTCTCG	40	6.8193913E-9	45.000004	1
TAGCCGT	20	7.034687E-4	45.000004	44
CGTTACA	20	7.034687E-4	45.000004	20
ATATCGC	30	2.1664073E-6	45.000004	22
CGAGGTG	60	0.0	45.000004	4
TACGACT	40	6.8193913E-9	45.000004	17
TATTCGA	40	6.8193913E-9	45.000004	28
GACGGGT	40	6.8193913E-9	45.000004	4
GACGGAT	20	7.034687E-4	45.000004	39
GTATACG	55	1.8189894E-12	45.000004	1
CGGTCTA	155	0.0	45.000004	31
CCGCGTA	20	7.034687E-4	45.000004	12
ACGTAGC	20	7.034687E-4	45.000004	23
TAGGCCG	55	1.8189894E-12	45.000004	1
CACACGG	70	0.0	45.000004	2
ACTCCGA	35	1.2126111E-7	45.000004	31
TATACGA	40	6.8193913E-9	45.000004	15
>>END_MODULE