FastQCFastQC Report
Sat 18 Jun 2016
SRR3548951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688666
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125950.7458550121812129No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20020.11855511984015786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAG453.8562575E-1045.0000041
GTCGACG207.034228E-445.01
TACGCTA253.8915863E-545.01
TCTACCG207.034228E-445.032
TCGCTAA207.034228E-445.012
CAGTCCG551.8189894E-1245.038
CGCAACA253.8915863E-545.027
TTCGACA207.034228E-445.012
ACGACCG406.8175723E-945.01
TGCGTCG207.034228E-445.02
TACGGGT1150.044.9999964
ACGGGTA1250.044.9999965
CGTTTTT97500.042.8769231
ACGTTCG800.042.187513
GGCGATT6550.041.9083988
ACGGCAT603.6379788E-1241.24999645
GGATGCC28600.041.1451038
TTTACGG1150.041.0869562
TAGCGGA1150.041.0869563
CACGACG1150.041.08695626