##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688666
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.86527708854208	31.0	31.0	33.0	30.0	33.0
2	31.19048112533799	31.0	31.0	33.0	30.0	34.0
3	31.277121704351245	31.0	31.0	34.0	28.0	34.0
4	35.15862402630242	35.0	35.0	37.0	32.0	37.0
5	35.06897160243648	37.0	35.0	37.0	32.0	37.0
6	35.155915971542036	37.0	35.0	37.0	32.0	37.0
7	35.54106732770128	36.0	35.0	37.0	35.0	37.0
8	35.593249938116834	37.0	35.0	37.0	35.0	37.0
9	37.3647974199753	39.0	37.0	39.0	35.0	39.0
10	36.86695888944291	39.0	37.0	39.0	32.0	39.0
11	36.54431012408611	38.0	35.0	39.0	32.0	39.0
12	35.603312318717855	37.0	35.0	39.0	31.0	39.0
13	35.257570768879106	37.0	35.0	39.0	30.0	39.0
14	36.000051519957175	38.0	35.0	40.0	30.0	41.0
15	36.319639881421196	38.0	35.0	40.0	31.0	41.0
16	36.40695081206112	38.0	35.0	40.0	32.0	41.0
17	36.21860510012045	37.0	35.0	40.0	31.0	41.0
18	36.2425796457085	37.0	35.0	40.0	31.0	41.0
19	36.12743254142619	37.0	35.0	40.0	31.0	41.0
20	35.88566241044706	36.0	34.0	40.0	31.0	41.0
21	35.756853634762585	36.0	34.0	40.0	30.0	41.0
22	35.60900497789379	36.0	34.0	40.0	30.0	41.0
23	35.48567449098874	35.0	34.0	40.0	30.0	41.0
24	35.57134448138353	35.0	34.0	40.0	30.0	41.0
25	35.42923941146444	35.0	34.0	40.0	30.0	41.0
26	35.16867574760195	35.0	34.0	40.0	29.0	41.0
27	35.158333264245265	35.0	34.0	40.0	29.0	41.0
28	35.1724029500209	36.0	34.0	40.0	29.0	41.0
29	35.13277758893707	36.0	34.0	40.0	29.0	41.0
30	35.092279941681774	36.0	34.0	40.0	29.0	41.0
31	34.91717189781757	36.0	34.0	40.0	28.0	41.0
32	34.74549022719709	35.0	34.0	40.0	27.0	41.0
33	34.47358506655549	35.0	33.0	40.0	25.0	41.0
34	34.166636860101406	35.0	33.0	40.0	24.0	41.0
35	33.90048416916075	35.0	33.0	40.0	23.0	41.0
36	33.68360824461439	35.0	33.0	40.0	22.0	41.0
37	33.71537177867027	35.0	33.0	40.0	23.0	41.0
38	33.708079632088285	35.0	33.0	40.0	23.0	41.0
39	33.68939091566953	35.0	33.0	40.0	23.0	41.0
40	33.40851299191196	35.0	32.0	40.0	21.0	41.0
41	33.402546743997924	35.0	32.0	40.0	21.0	41.0
42	33.38231953506496	35.0	32.0	40.0	21.0	41.0
43	33.33487498415909	35.0	32.0	40.0	21.0	41.0
44	33.28216118521958	35.0	32.0	40.0	21.0	41.0
45	33.33891900470549	35.0	32.0	40.0	22.0	41.0
46	33.36599481484201	35.0	33.0	40.0	22.0	41.0
47	33.27988897745321	35.0	32.0	40.0	21.0	41.0
48	33.30093754478387	35.0	32.0	39.0	21.0	41.0
49	33.32197426844622	35.0	32.0	39.0	21.0	41.0
50	33.17337294645596	35.0	32.0	39.0	20.0	41.0
51	32.88865116014653	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	5.0
12	2.0
13	6.0
14	15.0
15	52.0
16	188.0
17	492.0
18	1158.0
19	2421.0
20	4127.0
21	6607.0
22	9962.0
23	14532.0
24	20354.0
25	28685.0
26	36236.0
27	40887.0
28	43286.0
29	47000.0
30	54802.0
31	65637.0
32	82188.0
33	106312.0
34	179384.0
35	252604.0
36	110854.0
37	134904.0
38	192554.0
39	253314.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.3240557931527	23.264636109212837	28.006840902819146	15.404467194815316
2	34.79450643288845	25.45618849434998	27.75865683326365	11.990648239497924
3	28.25751214272094	25.002635216200243	33.90463241398832	12.835220227090497
4	25.605122623419906	27.03062654189757	33.69405199133517	13.670198843347354
5	21.610727047266895	32.12405531940597	32.97200275246851	13.29321488085862
6	20.015029615092622	40.25733922516353	31.057296114210864	8.670335045532983
7	82.9345767605909	5.298797986102639	10.085357317551251	1.6812679357552054
8	84.47881345393345	3.8528045214388165	9.885258541357498	1.7831234832702263
9	78.77466591972599	6.209576079579976	12.151662910249867	2.8640950904441733
10	49.06630440833178	23.228927449241	18.195664506776353	9.509103635650863
11	44.882528575810724	20.15975924191048	24.44704873551075	10.51066344676804
12	41.57559872704253	22.181591860083643	25.925789943067485	10.317019469806345
13	18.561456202706754	44.26636173168643	25.23850187070741	11.933680194899406
14	12.97165928608736	44.41428914894953	29.400070825136527	13.213980739826587
15	11.62509341693384	24.513017968029203	52.07216820851489	11.78972040652207
16	12.398958704681684	19.321938145257857	50.6051522325907	17.673950917469767
17	13.071915938379764	19.94414526022316	33.037379801571184	33.946558999825896
18	16.865620554923236	23.757924894561743	42.70317516903875	16.673279381476267
19	29.986332406763683	21.510470394974497	30.892017722865265	17.611179475396554
20	30.271527939805743	22.075591028658124	31.43108228625436	16.22179874528178
21	17.24248608072881	32.43507004937625	32.80216454882138	17.520279321073556
22	18.95330396893169	25.582264343570603	25.76963117632498	29.694800511172726
23	14.477996240819676	33.28911697162138	28.912052472188105	23.32083431537083
24	12.84617562028252	22.30754927262111	51.075819611456616	13.770455495639752
25	12.911493451043604	26.36234755718419	44.15633405303358	16.569824938738627
26	12.585318825629225	37.10348878937575	31.779759881468568	18.531432503526453
27	12.361355057779337	43.49368081077016	29.19185913614652	14.953104995303987
28	11.606676512702926	32.12648327141069	42.03981130667639	14.22702890920999
29	12.363013171343534	25.54460147832668	40.493738844744904	21.59864650558488
30	15.344715888162607	39.017603244217625	31.127706722347696	14.509974145272068
31	28.18206797554993	30.312803123885956	28.816888597271458	12.688240303292659
32	30.477134021766293	23.807549864804525	31.668429399300983	14.046886714128192
33	31.220561081942787	27.978120007153574	24.924822315366093	15.876496595537542
34	18.58603181446183	26.689469676063826	28.493852543960735	26.230645965513606
35	18.581057473769235	25.76684791427079	35.95287641250549	19.699218199454478
36	32.30259861926515	27.692509945720467	25.705438494053883	14.299452940960498
37	16.702059495483418	41.81389333355441	28.72107332059744	12.762973850364725
38	16.509126138620665	39.896048123193104	26.347779845155884	17.247045893030357
39	19.223576479895964	38.70457509063367	27.67877129047426	14.393077138996107
40	25.710531271429637	26.79327942885094	27.71939507279711	19.776794226922316
41	15.48002979866948	25.50504362615224	29.32918647026706	29.68574010491121
42	16.457724618130523	24.41862393155307	27.61084785268372	31.51280359763269
43	17.516844657262006	23.96133989788389	30.33749717232419	28.18431827252991
44	13.715915403045956	30.799281799953338	35.21596336990263	20.268839427098076
45	13.048406256773099	46.31774430230727	24.866788340619163	15.767061100300475
46	17.564574640574275	40.64077798688432	26.120914378568642	15.673732993972758
47	17.496355111075843	30.270698883023638	27.64412856065083	24.588817445249685
48	17.028352557580956	27.927784416811853	38.50725957649411	16.536603449113088
49	19.941006688119497	28.82221824801352	36.617661515065734	14.619113548801243
50	17.517969805751996	40.177572119057295	27.726501273786525	14.577956801404184
51	16.436109923454374	36.98108447733299	27.083330865902433	19.499474733310198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1470.0
1	2812.0
2	4154.0
3	13995.5
4	23837.0
5	18168.5
6	12500.0
7	12409.0
8	12318.0
9	12737.5
10	13157.0
11	12942.5
12	12728.0
13	12458.0
14	12188.0
15	11514.0
16	10840.0
17	10551.0
18	10262.0
19	10239.5
20	10217.0
21	10954.0
22	11691.0
23	11952.0
24	12213.0
25	14780.0
26	20552.5
27	23758.0
28	29143.0
29	34528.0
30	41583.5
31	48639.0
32	57618.5
33	66598.0
34	71594.0
35	76590.0
36	81280.5
37	85971.0
38	98337.0
39	110703.0
40	142557.5
41	174412.0
42	192990.5
43	211569.0
44	200197.5
45	188826.0
46	163230.0
47	137634.0
48	121661.5
49	105689.0
50	89762.5
51	73836.0
52	58949.0
53	44062.0
54	36198.0
55	28334.0
56	26037.0
57	23740.0
58	22226.5
59	20713.0
60	19655.5
61	18598.0
62	16159.5
63	13721.0
64	12302.5
65	10884.0
66	9173.0
67	7462.0
68	6787.5
69	6113.0
70	5234.0
71	4355.0
72	3705.0
73	3055.0
74	2501.0
75	1365.5
76	784.0
77	678.0
78	572.0
79	455.0
80	338.0
81	242.5
82	147.0
83	117.5
84	88.0
85	60.5
86	33.0
87	27.5
88	22.0
89	15.5
90	9.0
91	6.5
92	4.0
93	6.5
94	9.0
95	5.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1688666.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.124720399401983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.99477294200322	15.897477184720602
2	6.679651603230688	2.688522417608696
3	2.7340825937599917	1.6506794324487477
4	1.6700950887927926	1.344407868094775
5	1.221500773533536	1.2291180767507828
6	0.9322310933861325	1.125653406121483
7	0.7213741409157034	1.0162217022501137
8	0.5938496361823965	0.9560846309965856
9	0.511287034609893	0.9260557753827117
>10	4.093446929169783	16.801462803460687
>50	0.7602478692754955	10.98615127046046
>100	1.0661781148605456	41.66535777162891
>500	0.01862190774489521	2.3352944503603537
>1k	0.002364686697764471	0.6282892654925379
>5k	0.0	0.0
>10k+	2.955858372205589E-4	0.7492239442225445
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12595	0.7458550121812129	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2002	0.11855511984015786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.015100677102517609	0.0
2	0.0	0.0	0.0	0.057441791331145416	0.0
3	0.0	0.0	0.0	0.08562972192251161	0.0
4	0.0	0.0	0.0	0.12915520298270944	0.0
5	0.0	0.0	0.0	0.22929341859195365	0.0
6	0.0	0.0	0.0	0.353118970832598	0.0
7	0.0	0.0	0.0	0.4404068063193077	0.0
8	0.0	0.0	0.0	0.6609359103576432	0.0
9	0.0	0.0	0.0	0.7603042875263669	0.0
10	0.0	0.0	0.0	0.9140943206057326	0.0
11	0.0	0.0	0.0	1.0264907329217263	0.0
12	0.0	0.0	0.0	1.1326100010303992	0.0
13	0.0	0.0	0.0	1.17838577907058	0.0
14	0.0	0.0	0.0	1.1941970762720395	0.0
15	0.0	0.0	0.0	1.2164631727055557	0.0
16	0.0	0.0	0.0	1.2680423482204297	0.0
17	0.0	0.0	0.0	1.3300439518531195	0.0
18	0.0	0.0	0.0	1.4311296609276198	0.0
19	0.0	0.0	0.0	1.472345626666256	0.0
20	0.0	0.0	0.0	1.5219705969090394	0.0
21	0.0	0.0	0.0	1.5826101786854239	0.0
22	0.0	0.0	0.0	1.6488162845701873	0.0
23	0.0	0.0	0.0	1.728524172334849	0.0
24	0.0	0.0	0.0	1.789341409135969	0.0
25	0.0	0.0	0.0	1.8413943313834706	0.0
26	0.0	0.0	0.0	1.8939210003636007	0.0
27	0.0	0.0	0.0	1.9481057829079285	0.0
28	0.0	0.0	0.0	2.0035341506254047	0.0
29	0.0	0.0	0.0	2.074536942178027	0.0
30	0.0	0.0	0.0	2.1779913849156673	0.0
31	0.0	0.0	0.0	2.2683585741644587	0.0
32	0.0	0.0	0.0	2.3500798855427893	0.0
33	0.0	0.0	0.0	2.429550899941137	0.0
34	0.0	0.0	0.0	2.5144700017647064	0.0
35	0.0	0.0	0.0	2.628820619352791	0.0
36	0.0	0.0	0.0	2.716404546547393	0.0
37	0.0	0.0	0.0	2.811568421464043	0.0
38	0.0	0.0	0.0	2.9085088466280484	0.0
39	0.0	0.0	0.0	3.0343478224823617	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAG	45	3.8562575E-10	45.000004	1
GTCGACG	20	7.034228E-4	45.0	1
TACGCTA	25	3.8915863E-5	45.0	1
TCTACCG	20	7.034228E-4	45.0	32
TCGCTAA	20	7.034228E-4	45.0	12
CAGTCCG	55	1.8189894E-12	45.0	38
CGCAACA	25	3.8915863E-5	45.0	27
TTCGACA	20	7.034228E-4	45.0	12
ACGACCG	40	6.8175723E-9	45.0	1
TGCGTCG	20	7.034228E-4	45.0	2
TACGGGT	115	0.0	44.999996	4
ACGGGTA	125	0.0	44.999996	5
CGTTTTT	9750	0.0	42.876923	1
ACGTTCG	80	0.0	42.1875	13
GGCGATT	655	0.0	41.908398	8
ACGGCAT	60	3.6379788E-12	41.249996	45
GGATGCC	2860	0.0	41.145103	8
TTTACGG	115	0.0	41.086956	2
TAGCGGA	115	0.0	41.086956	3
CACGACG	115	0.0	41.086956	26
>>END_MODULE