##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548949_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2381836
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.82891475315681	31.0	31.0	33.0	28.0	33.0
2	31.143634154492585	31.0	31.0	33.0	30.0	34.0
3	31.255372326222293	31.0	31.0	34.0	28.0	34.0
4	35.137360422799894	35.0	35.0	37.0	32.0	37.0
5	35.02671804439936	37.0	35.0	37.0	32.0	37.0
6	35.113596402103255	37.0	35.0	37.0	32.0	37.0
7	35.505367288092046	36.0	35.0	37.0	35.0	37.0
8	35.55633385338033	37.0	35.0	37.0	35.0	37.0
9	37.32978173140384	39.0	37.0	39.0	35.0	39.0
10	36.82536790946144	39.0	37.0	39.0	32.0	39.0
11	36.50322482320361	38.0	35.0	39.0	32.0	39.0
12	35.551744956411774	37.0	35.0	39.0	31.0	39.0
13	35.18647211646814	37.0	35.0	39.0	30.0	39.0
14	35.92661627416833	38.0	35.0	40.0	30.0	41.0
15	36.23334142233134	38.0	35.0	40.0	31.0	41.0
16	36.3297897084434	37.0	35.0	40.0	31.0	41.0
17	36.124108460868	37.0	34.0	40.0	31.0	41.0
18	36.178058438952135	37.0	35.0	40.0	31.0	41.0
19	36.074919096025084	37.0	35.0	40.0	31.0	41.0
20	35.83170881622412	36.0	34.0	40.0	30.0	41.0
21	35.71520415343458	36.0	34.0	40.0	30.0	41.0
22	35.53957325357413	36.0	34.0	40.0	30.0	41.0
23	35.41255191373377	35.0	34.0	40.0	29.0	41.0
24	35.47822939950525	35.0	34.0	40.0	30.0	41.0
25	35.34805419012896	35.0	34.0	40.0	30.0	41.0
26	35.08447726879601	35.0	34.0	40.0	29.0	41.0
27	35.093046288661355	35.0	34.0	40.0	29.0	41.0
28	35.099310783781924	36.0	34.0	40.0	29.0	41.0
29	35.0583180370101	36.0	34.0	40.0	29.0	41.0
30	35.01865241771474	36.0	34.0	40.0	29.0	41.0
31	34.819021964568506	36.0	34.0	40.0	27.0	41.0
32	34.66755057862926	35.0	34.0	40.0	27.0	41.0
33	34.40520631983058	35.0	33.0	40.0	25.0	41.0
34	34.12912769812867	35.0	33.0	40.0	24.0	41.0
35	33.89108905902842	35.0	33.0	40.0	23.0	41.0
36	33.68800076915455	35.0	33.0	40.0	22.0	41.0
37	33.71347565491495	35.0	33.0	40.0	23.0	41.0
38	33.70111796110228	35.0	33.0	40.0	23.0	41.0
39	33.68322882012028	35.0	33.0	40.0	23.0	41.0
40	33.43622482824174	35.0	32.0	40.0	22.0	41.0
41	33.436902456760244	35.0	32.0	40.0	22.0	41.0
42	33.40176233796114	35.0	32.0	40.0	22.0	41.0
43	33.321826943584696	35.0	32.0	40.0	21.0	41.0
44	33.286090226195256	35.0	32.0	40.0	21.0	41.0
45	33.352081335574745	35.0	32.0	39.0	22.0	41.0
46	33.34757262884598	35.0	32.0	39.0	22.0	41.0
47	33.25686193339928	35.0	32.0	39.0	22.0	41.0
48	33.24956042313577	35.0	32.0	39.0	22.0	41.0
49	33.257317464342634	35.0	32.0	39.0	21.0	41.0
50	33.08497772306742	35.0	32.0	39.0	20.0	41.0
51	32.82681679175224	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	4.0
11	3.0
12	3.0
13	11.0
14	25.0
15	76.0
16	261.0
17	680.0
18	1702.0
19	3349.0
20	5930.0
21	9361.0
22	13929.0
23	20078.0
24	28807.0
25	39725.0
26	50852.0
27	58384.0
28	61909.0
29	68281.0
30	79411.0
31	95133.0
32	117653.0
33	151317.0
34	254303.0
35	362950.0
36	155658.0
37	189760.0
38	267108.0
39	345054.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.367368702127266	23.73198658513852	28.24476580251537	14.655878910218838
2	33.05114206015863	26.363989796106868	28.65902606224778	11.925842081486719
3	29.20247237845091	24.827192132455803	33.44890244332524	12.521433045768054
4	26.362940185638305	27.139022166093717	32.47557766361748	14.022459984650496
5	22.53328944562094	31.585382033019904	32.05833651015435	13.822992011204802
6	19.895450400447388	41.709588737427765	29.187735847472286	9.207225014652561
7	83.2648007671393	5.197041274042377	9.73795005197671	1.800207906841613
8	84.43851717750508	4.298196853183846	9.40522353344227	1.8580624358688003
9	78.8095402034397	6.864956277426322	11.52241380178988	2.803089717344099
10	49.82131431383185	23.458206190518574	17.317187245469462	9.403292250180113
11	45.97869038842305	20.218436533833565	23.179639572161978	10.623233505581409
12	42.398679002248684	22.376183750686447	24.83202034061119	10.393116906453677
13	18.96919015414999	44.43907976871623	23.982801502706316	12.608928574427459
14	12.726526931325246	45.965339343262926	28.054156541424348	13.253977183987478
15	12.267301359119603	24.63922788974556	51.42423743700238	11.669233314132459
16	13.148302402012565	19.790069509403672	49.676300131495196	17.385327957088563
17	12.884640252309563	19.94591567177589	32.818926240093774	34.35051783582077
18	16.622471068537045	23.54738109592768	42.20017667043407	17.629971165101207
19	30.575950653193583	20.86109203152526	29.723331077370567	18.839626237910586
20	30.484844464522325	22.37509215579914	30.508313754599392	16.631749625079138
21	18.219138513314938	32.785254736262274	31.812391785160692	17.183214965262092
22	19.919297550293138	26.054438676718295	24.912672409015567	29.113591363973
23	15.78479794578636	32.65909155794102	27.341093173501452	24.215017322771175
24	13.094898221372084	23.506782163003667	49.34525298971046	14.05306662591379
25	13.049387111455196	27.03951909367396	43.888202210395676	16.02289158447517
26	12.220236825709243	37.57739827595183	31.26764395197654	18.934720946362386
27	12.958700766971361	42.98167464090727	28.719693547330717	15.339931044790658
28	11.443441110135206	33.17436632916792	40.567906438562524	14.814286122134353
29	12.109775819997683	25.823944217821882	40.14508135740664	21.921198604773796
30	15.341484468284131	38.568272542693954	31.085767449983965	15.004475539037953
31	28.461111512295556	31.131950310600732	27.42472613563654	12.98221204146717
32	31.241067814912533	24.18428472825165	29.84512787614261	14.729519580693212
33	30.44957755277861	28.723808020367482	24.121643975487817	16.704970451366087
34	19.100391462720356	27.209933849349827	27.609583531359842	26.080091156569974
35	19.49521293657498	25.430508229785765	34.77838104722576	20.295897786413505
36	31.841109127580573	28.529840005777057	25.42681360093642	14.202237265705953
37	16.758752491775255	41.25783639175829	29.557366670081397	12.426044446385058
38	16.60009337334728	39.651638483925844	25.926931996997276	17.821336145729596
39	19.655131587565222	37.60023779974776	27.566759424242477	15.177871188444545
40	25.052480523428144	26.14059070397794	27.620919324420324	21.186009448173593
41	14.729561565111956	25.976095751344765	29.4555544546308	29.838788228912488
42	15.837110531539537	24.683689389193884	27.675373115529368	31.80382696373722
43	17.23888630451467	23.46895420171666	30.331937211462083	28.960222282306592
44	13.734572825333064	30.393234462826157	34.20365633905945	21.668536372781332
45	12.795927175506627	46.27098591170844	24.42187455391555	16.511212358869376
46	17.84757640744367	40.50639926510474	25.629136514856604	16.01688781259499
47	18.095452415699487	30.544504323555444	27.552442737451276	23.807600523293797
48	17.304130091240538	28.09819819668525	37.66342434995524	16.93424736211897
49	20.72342512246855	27.919512510517098	36.62468784584665	14.732374521167705
50	17.719649883535222	40.32502657613706	27.16156779895845	14.793755741369264
51	16.194691825969546	36.62006955978497	27.21228497679941	19.97295363744607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2306.0
1	4265.0
2	6224.0
3	18594.5
4	30965.0
5	23586.5
6	16208.0
7	16360.5
8	16513.0
9	16740.0
10	16967.0
11	17015.5
12	17064.0
13	16766.0
14	16468.0
15	15890.5
16	15313.0
17	13942.5
18	12572.0
19	12791.0
20	13010.0
21	13408.5
22	13807.0
23	15411.0
24	17015.0
25	21430.0
26	30611.0
27	35377.0
28	44132.0
29	52887.0
30	60374.0
31	67861.0
32	76945.5
33	86030.0
34	96380.0
35	106730.0
36	106335.5
37	105941.0
38	124318.5
39	142696.0
40	189237.0
41	235778.0
42	271700.5
43	307623.0
44	291186.0
45	274749.0
46	234674.5
47	194600.0
48	169075.5
49	143551.0
50	125696.0
51	107841.0
52	87563.5
53	67286.0
54	57985.0
55	48684.0
56	44906.0
57	41128.0
58	38041.0
59	34954.0
60	32907.5
61	30861.0
62	26983.0
63	23105.0
64	19738.0
65	16371.0
66	14000.0
67	11629.0
68	9945.5
69	8262.0
70	7559.0
71	6856.0
72	5920.0
73	4984.0
74	3958.5
75	2133.0
76	1333.0
77	968.5
78	604.0
79	533.0
80	462.0
81	344.0
82	226.0
83	176.5
84	127.0
85	88.5
86	50.0
87	32.5
88	15.0
89	12.5
90	10.0
91	6.0
92	2.0
93	7.0
94	12.0
95	6.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2381836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.395957688632308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62658585829939	15.444338901978227
2	6.918022869830796	2.683633577444576
3	2.6602076537368835	1.547918252845693
4	1.555349579659896	1.2066997855246155
5	1.1137339328802467	1.080096811926966
6	0.8135967998730145	0.9468293463566181
7	0.677474186414036	0.9198182458379066
8	0.5549006780991926	0.8610264059044255
9	0.461452417763735	0.8055280413236237
>10	4.066153315182877	15.824017066982815
>50	0.5643973269409652	7.843471108027401
>100	0.9409163975476488	43.09842235909386
>500	0.04177016075619943	5.198985171747317
>1k	0.005003717173919724	1.6111035138868455
>5k	2.1755292060520536E-4	0.25381178167228136
>10k+	2.1755292060520536E-4	0.6742996294468921
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15980	0.6709110115054101	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6015	0.2525362787362354	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3797	0.15941483796533432	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3135	0.13162115275778852	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.198441874251628E-5	0.0	0.0	0.024266994033174408	0.0
2	4.198441874251628E-5	0.0	0.0	0.07439639001173884	0.0
3	4.198441874251628E-5	0.0	0.0	0.10710225221215902	0.0
4	4.198441874251628E-5	0.0	0.0	0.1578194300531187	0.0
5	4.198441874251628E-5	0.0	0.0	0.28280704464958967	0.0
6	4.198441874251628E-5	0.0	0.0	0.42026403161258796	0.0
7	4.198441874251628E-5	0.0	0.0	0.5207327456634294	0.0
8	4.198441874251628E-5	0.0	0.0	0.8035817747317615	0.0
9	4.198441874251628E-5	0.0	0.0	0.9206763186046395	0.0
10	4.198441874251628E-5	0.0	0.0	1.1043581506031481	0.0
11	4.198441874251628E-5	0.0	0.0	1.2452998443217753	0.0
12	4.198441874251628E-5	0.0	0.0	1.3761652775421986	0.0
13	4.198441874251628E-5	0.0	0.0	1.4278900814329787	0.0
14	4.198441874251628E-5	0.0	0.0	1.4480426024293864	0.0
15	4.198441874251628E-5	0.0	0.0	1.4787751969489082	0.0
16	4.198441874251628E-5	0.0	0.0	1.5512403036984914	0.0
17	4.198441874251628E-5	0.0	0.0	1.6348312814148414	0.0
18	4.198441874251628E-5	0.0	0.0	1.7701050786032289	0.0
19	4.198441874251628E-5	0.0	0.0	1.8274138101867634	0.0
20	8.396883748503256E-5	0.0	0.0	1.8865698561949689	0.0
21	8.396883748503256E-5	0.0	0.0	1.972302039267187	0.0
22	8.396883748503256E-5	0.0	0.0	2.0631983058447347	0.0
23	8.396883748503256E-5	0.0	0.0	2.169334916425816	0.0
24	8.396883748503256E-5	0.0	0.0	2.253513676004561	0.0
25	8.396883748503256E-5	0.0	0.0	2.3257268762416894	0.0
26	8.396883748503256E-5	0.0	0.0	2.402810269052949	0.0
27	8.396883748503256E-5	0.0	0.0	2.474015843240257	0.0
28	8.396883748503256E-5	0.0	0.0	2.55332441024487	0.0
29	8.396883748503256E-5	0.0	0.0	2.645648147059663	0.0
30	8.396883748503256E-5	0.0	0.0	2.769670120025056	0.0
31	8.396883748503256E-5	0.0	0.0	2.8827341596986527	0.0
32	8.396883748503256E-5	0.0	0.0	2.9860578142239853	0.0
33	8.396883748503256E-5	0.0	0.0	3.096392866679318	0.0
34	8.396883748503256E-5	0.0	0.0	3.211933987058723	0.0
35	8.396883748503256E-5	0.0	0.0	3.3530856028710625	0.0
36	8.396883748503256E-5	0.0	0.0	3.481389986548192	0.0
37	8.396883748503256E-5	0.0	0.0	3.6051600530011303	0.0
38	8.396883748503256E-5	0.0	0.0	3.726075178979577	0.0
39	8.396883748503256E-5	0.0	0.0	3.8524902638132934	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAA	20	7.0348114E-4	45.000004	28
CACGATA	20	7.0348114E-4	45.000004	22
CGAATCG	20	7.0348114E-4	45.000004	12
ACGAACT	20	7.0348114E-4	45.000004	42
TGTAACG	35	1.2126657E-7	45.0	1
ACGTTAG	60	0.0	44.999996	1
CGCTAAT	30	2.1664873E-6	44.999996	11
GACGACT	25	3.8920713E-5	44.999996	4
TCGAATA	25	3.8920713E-5	44.999996	23
CGGTCTA	200	0.0	42.749996	31
ACGGGAC	285	0.0	42.63158	5
CGTTTTT	12480	0.0	42.16947	1
TCACGAC	210	0.0	41.785717	25
CTCACGA	210	0.0	41.785717	24
TAGTTCG	70	0.0	41.785713	1
AATGCGG	410	0.0	41.707317	2
ATATCGA	65	0.0	41.538464	14
GCTACGA	190	0.0	41.44737	10
ACGGGAT	755	0.0	41.42384	5
TTGTGCG	125	0.0	41.399998	1
>>END_MODULE