FastQCFastQC Report
Sat 18 Jun 2016
SRR3548948_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548948_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643167
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48380.29443142419486273No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC25680.15628356703852986No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG22590.13747841820094975No Hit
GGTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA20680.12585452361202484No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG20460.1245156457012586No Hit
GTAGATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAAT18810.11447406137051194No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG18180.11064000189877231No Hit
GTATAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA16490.1003549852206136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA406.8175723E-945.00000435
ACGCGAG302.1660726E-645.0000041
AGTTTCG302.1660726E-645.0000041
CGAAATT406.8175723E-945.00000437
GTACAAT207.034172E-445.00000413
CCCTAGA207.034172E-445.00000444
TAGACCG406.8175723E-945.0000041
GGCACGA406.8175723E-945.00000433
ACACCGT302.1660726E-645.00000435
GGCGTTA351.2123564E-745.00000420
CGACCTT207.034172E-445.00000423
ATTCGGT302.1660726E-645.00000430
TACGGTG351.2123564E-745.0000044
ACCTCGT302.1660726E-645.00000437
CGTTATC351.2123564E-745.00000422
CCGTATG600.045.0000041
CCCGTAC302.1660726E-645.0000044
CCGTAGA207.034172E-445.00000433
TACGCGG207.034172E-445.0000042
CCGTAAG800.045.0000041