FastQCFastQC Report
Sat 18 Jun 2016
SRR3548946_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548946_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1693876
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66530.3927678295223499No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC23060.1361374740535907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22110.13052903518321293No Hit
TAGAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA20240.11948926603836409No Hit
ATTTGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA19260.11370371857207966No Hit
GTTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA17330.10230973223541748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG302.1661126E-645.0000041
TCGCAAA302.1661126E-645.00000431
CGAACGA302.1661126E-645.00000422
GATCGAA302.1661126E-645.00000413
GTACAAC302.1661126E-645.00000431
TCCGATA302.1661126E-645.00000414
TAGCGCT302.1661126E-645.00000429
GACCGAA302.1661126E-645.00000436
ATTAACG302.1661126E-645.0000041
CGATTCG650.045.00000410
AGTGATC302.1661126E-645.00000410
AGCGCGA302.1661126E-645.0000045
CTACCGG650.045.0000042
TACTCCG302.1661126E-645.00000434
CGTGTAC302.1661126E-645.00000418
TATCGAG1350.045.0000041
AATCGTA302.1661126E-645.00000413
ACGCGAG1050.045.01
CGGACAC207.034234E-445.027
TCACGAC700.045.025
Produced by FastQC (version 0.11.5)