##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548946_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1693876
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.827787866408165	31.0	31.0	33.0	28.0	33.0
2	31.132146627025826	31.0	31.0	33.0	30.0	34.0
3	31.284960056108	31.0	31.0	34.0	28.0	34.0
4	35.13035723984518	35.0	35.0	37.0	32.0	37.0
5	34.982688225112106	35.0	35.0	37.0	32.0	37.0
6	35.08699987484326	37.0	35.0	37.0	32.0	37.0
7	35.524037178636455	36.0	35.0	37.0	35.0	37.0
8	35.601953743957644	37.0	35.0	37.0	35.0	37.0
9	37.43837624477825	39.0	37.0	39.0	35.0	39.0
10	36.930109405883314	39.0	37.0	39.0	32.0	39.0
11	36.464779594256015	38.0	35.0	39.0	32.0	39.0
12	34.83286438912884	35.0	34.0	39.0	30.0	39.0
13	34.240759063827575	35.0	33.0	39.0	26.0	39.0
14	34.7990307436908	35.0	33.0	40.0	26.0	41.0
15	35.354387216065405	35.0	33.0	40.0	30.0	41.0
16	35.62293520895272	35.0	34.0	40.0	31.0	41.0
17	35.49231112548971	35.0	34.0	40.0	31.0	41.0
18	35.617673312568336	36.0	34.0	39.0	31.0	41.0
19	35.45291331833027	36.0	34.0	39.0	30.0	41.0
20	35.153594477990126	35.0	34.0	39.0	30.0	41.0
21	34.96662093329146	35.0	34.0	39.0	29.0	41.0
22	34.76322942175224	35.0	33.0	39.0	29.0	41.0
23	34.742984728516134	35.0	34.0	39.0	29.0	41.0
24	34.88458600275345	35.0	34.0	39.0	29.0	41.0
25	34.781804571290934	35.0	34.0	38.0	29.0	41.0
26	34.550942335802624	35.0	34.0	38.0	29.0	41.0
27	34.497992769246395	35.0	34.0	38.0	29.0	41.0
28	34.62305091990205	35.0	34.0	38.0	29.0	41.0
29	34.64472074697321	35.0	34.0	38.0	29.0	41.0
30	34.6189685667664	35.0	34.0	38.0	29.0	41.0
31	34.31329920253903	35.0	34.0	38.0	28.0	41.0
32	34.11928500079109	35.0	34.0	38.0	27.0	41.0
33	33.8447471951902	35.0	33.0	38.0	25.0	41.0
34	33.67056856582182	35.0	33.0	38.0	24.0	41.0
35	33.44633609544028	35.0	33.0	38.0	23.0	41.0
36	33.167781466884236	35.0	33.0	38.0	23.0	41.0
37	33.194504792558604	35.0	32.0	38.0	23.0	41.0
38	33.290257964573556	35.0	32.0	38.0	23.0	41.0
39	33.31490675822788	35.0	33.0	38.0	23.0	41.0
40	32.94770219307671	35.0	32.0	38.0	23.0	41.0
41	33.05710276313024	35.0	32.0	38.0	23.0	41.0
42	33.01019732259032	35.0	32.0	38.0	23.0	41.0
43	32.96312894214216	35.0	32.0	38.0	23.0	41.0
44	32.94847143474493	35.0	32.0	38.0	23.0	40.0
45	33.01251567411074	35.0	32.0	38.0	23.0	40.0
46	33.02130144119168	35.0	32.0	38.0	23.0	40.0
47	32.97089574443466	35.0	32.0	38.0	23.0	40.0
48	33.01790508868418	35.0	32.0	38.0	23.0	40.0
49	33.102923708701226	35.0	32.0	38.0	23.0	40.0
50	32.82089125768356	35.0	32.0	38.0	23.0	40.0
51	32.532564367167375	35.0	31.0	37.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	6.0
14	11.0
15	40.0
16	151.0
17	506.0
18	1160.0
19	2349.0
20	4133.0
21	6672.0
22	10121.0
23	14782.0
24	20326.0
25	28162.0
26	35501.0
27	41180.0
28	45026.0
29	50461.0
30	60281.0
31	73707.0
32	94454.0
33	125789.0
34	231694.0
35	339428.0
36	86055.0
37	99469.0
38	138443.0
39	183879.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.30800365552142	26.0839046069488	31.06083326052202	12.54725847700776
2	29.987968422718076	26.521598983632806	31.620260278792543	11.870172314856578
3	27.48825770009139	25.50529082412172	34.14464813244889	12.861803343338002
4	24.69129971733468	27.588205984381382	34.14984331792882	13.570650980355115
5	20.9580866604167	32.416009200201195	33.907853939721676	12.718050199660425
6	19.17176936210207	41.77265632195037	29.48793182027492	9.567642495672647
7	86.348174246521	4.922320169835336	7.301006685259133	1.4284988983845335
8	89.29543839100383	2.7829664036800805	6.815906241070775	1.1056889642453167
9	85.39981675163943	4.464671558012511	8.426000486458276	1.709511203889777
10	64.99678843079423	16.71893338119201	12.297594392977999	5.986683795035764
11	61.6759432213456	15.002928195452322	16.74290207783805	6.57822650536403
12	54.002654267490655	20.33342464265389	18.488484399094148	7.175436690761307
13	17.440355728518497	56.20694785214502	18.631883325579913	7.7208130937565675
14	9.010340780553005	57.168175238329134	25.514618543506135	8.306865437611725
15	7.532310511513239	22.053857543291244	62.16181113611622	8.252020809079296
16	8.411949871183015	15.251706736502554	60.599300066828974	15.737043325485455
17	8.737239325664925	15.38725384856979	30.717419693058996	45.15808713270629
18	15.055293303642062	21.001242121619292	47.68743402704803	16.25603054769062
19	35.542625316138846	16.6495068115966	29.661203063270275	18.146664808994284
20	35.59363259175996	19.138591018468883	28.56242133426532	16.705355055505834
21	16.632268241595018	36.08062219430466	31.446339637612198	15.840769926488123
22	18.870094387074378	23.453428704344358	20.84585884681051	36.83061806177076
23	11.28211274024781	35.918390720454155	25.047110886511177	27.752385652786867
24	9.77385593750664	19.827838637538996	58.63357176086089	11.764733664093475
25	8.760322479331426	22.293485473552963	53.109200437340164	15.836991609775449
26	8.05525315902699	43.382337313947424	30.663519643704735	17.898889883320855
27	8.977280509317092	53.85394208312769	25.704301849722178	11.46447555783304
28	7.5048586791477065	35.50141804949122	45.15991725486399	11.833806016497075
29	8.417676382450663	24.483020008548444	45.11823769862729	21.981065910373605
30	12.849818995014983	43.80887384908931	30.231551778288374	13.109755377607335
31	34.17995177923295	29.222623143606736	25.871787545251244	10.725637531909065
32	38.6473390023827	19.771222923047496	30.93349218006513	10.647945894504675
33	39.17535876297911	25.707371732051225	20.43478979571114	14.682479709258528
34	19.42745513839266	25.787306745003768	24.111032920945807	30.674205195657773
35	17.21040973483301	22.71789670554397	38.18113014175772	21.890563417865298
36	41.35019328451433	23.124774186540222	23.776061529887667	11.748970999057782
37	15.133634339231444	46.45216060679766	27.71879405576323	10.695410998207661
38	15.957071237800168	44.964271292585764	21.220266418557202	17.858391051056866
39	18.68235927541331	41.29074383248833	26.298146971797227	13.72874992030113
40	29.52329450325762	22.73613889092236	24.588812876503358	23.151753729316667
41	13.127348164800729	22.647997846359473	26.40157839180672	37.82307559703308
42	15.346400799113985	18.942236621807027	26.70974734868432	39.00161523039467
43	15.966871246773673	19.580417929057383	29.488935435651726	34.96377538851722
44	11.094259556189472	30.31986993144717	36.7167962708014	21.869074241561957
45	10.692282079679977	54.05791214941353	21.49195100467803	13.757854766228462
46	18.33770594777894	43.69328097216089	23.58395773952757	14.3850553405326
47	17.217789259662457	29.355218445742192	23.996266550798286	29.430725743797066
48	17.673666785526212	26.79062694081503	40.885991654642964	14.649714619015796
49	23.580946893397154	24.140019694475864	40.48714309666115	11.79189031546583
50	18.619603796263718	45.807013028108315	23.579411952232633	11.993971223395338
51	15.731434886615078	42.05726983557238	22.577685733784527	19.633609544028015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2903.0
1	3237.0
2	3571.0
3	8008.5
4	12446.0
5	10056.0
6	7666.0
7	8434.5
8	9203.0
9	9838.0
10	10473.0
11	10703.5
12	10934.0
13	10743.5
14	10553.0
15	10107.5
16	9662.0
17	8774.0
18	7886.0
19	7905.0
20	7924.0
21	6876.5
22	5829.0
23	8208.0
24	10587.0
25	11049.5
26	14173.0
27	16834.0
28	21571.5
29	26309.0
30	31087.5
31	35866.0
32	41996.5
33	48127.0
34	49709.0
35	51291.0
36	54315.5
37	57340.0
38	80270.5
39	103201.0
40	152918.5
41	202636.0
42	247052.5
43	291469.0
44	274956.0
45	258443.0
46	216124.0
47	173805.0
48	146196.0
49	118587.0
50	96773.5
51	74960.0
52	59437.0
53	43914.0
54	32585.0
55	21256.0
56	17052.0
57	12848.0
58	11619.0
59	10390.0
60	8983.5
61	7577.0
62	6719.0
63	5861.0
64	4984.0
65	4107.0
66	3433.5
67	2760.0
68	2313.0
69	1866.0
70	1580.5
71	1295.0
72	1112.0
73	929.0
74	759.5
75	423.5
76	257.0
77	180.5
78	104.0
79	76.5
80	49.0
81	38.0
82	27.0
83	22.0
84	17.0
85	10.5
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	2.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1693876.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.49741928900832
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07607456247067	13.375459963639802
2	7.80603312118818	2.575588027682554
3	2.593622265169784	1.283642219574403
4	1.3271834406612482	0.8758040677606922
5	0.8879943658948881	0.7324807690222519
6	0.6077959866274946	0.6016239140142162
7	0.45380003868909485	0.5240570658135537
8	0.3913373754124135	0.5164845412510912
9	0.345516404472741	0.5130116096235657
>10	2.914382955784951	10.148238579460193
>50	0.47927304992103115	5.56579598315651
>100	0.8935172919365315	38.75430816785239
>500	0.20766103527917779	20.713243839832355
>1k	0.015448831344298693	3.425930440117636
>5k	3.5927514754183014E-4	0.3943308111988138
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6653	0.3927678295223499	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2306	0.1361374740535907	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2211	0.13052903518321293	No Hit
TAGAAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2024	0.11948926603836409	No Hit
ATTTGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1926	0.11370371857207966	No Hit
GTTTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1733	0.10230973223541748	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03489039339361323	0.0
2	0.0	0.0	0.0	0.13714108943039513	0.0
3	0.0	0.0	0.0	0.19257607994918163	0.0
4	0.0	0.0	0.0	0.2761123010184925	0.0
5	0.0	0.0	0.0	0.4740606750435097	0.0
6	0.0	0.0	0.0	0.6511692709501758	0.0
7	0.0	0.0	0.0	0.7600910574327755	0.0
8	0.0	0.0	0.0	1.0236286481418946	0.0
9	0.0	0.0	0.0	1.1139540320542944	0.0
10	0.0	0.0	0.0	1.2571758499441517	0.0
11	0.0	0.0	0.0	1.4242482920827735	0.0
12	0.0	0.0	0.0	1.5595592593554664	0.0
13	0.0	0.0	0.0	1.6169424444292262	0.0
14	0.0	0.0	0.0	1.6365424623762306	0.0
15	0.0	0.0	0.0	1.6758605706675105	0.0
16	0.0	0.0	0.0	1.7651233030044702	0.0
17	0.0	0.0	0.0	1.86979448318531	0.0
18	0.0	0.0	0.0	2.0535151333391584	0.0
19	0.0	0.0	0.0	2.119812784406887	0.0
20	0.0	0.0	0.0	2.1881176662282247	0.0
21	0.0	0.0	0.0	2.2923755930186154	0.0
22	0.0	0.0	0.0	2.3947443614526684	0.0
23	0.0	0.0	0.0	2.5252733966358814	0.0
24	0.0	0.0	0.0	2.6260481877067745	0.0
25	0.0	0.0	0.0	2.7039759699057075	0.0
26	0.0	0.0	0.0	2.7951868967976403	0.0
27	0.0	0.0	0.0	2.8786050454696803	0.0
28	0.0	0.0	0.0	2.964030424895329	0.0
29	0.0	0.0	0.0	3.06297509380852	0.0
30	0.0	0.0	0.0	3.2028318483761504	0.0
31	0.0	0.0	0.0	3.3406223359915366	0.0
32	0.0	0.0	0.0	3.4568646111049453	0.0
33	0.0	0.0	0.0	3.569623750498856	0.0
34	0.0	0.0	0.0	3.6882274735576868	0.0
35	0.0	0.0	0.0	3.8642734178889127	0.0
36	0.0	0.0	0.0	3.996219321839379	0.0
37	0.0	0.0	0.0	4.13341944746841	0.0
38	0.0	0.0	0.0	4.2642436636448	0.0
39	0.0	0.0	0.0	4.382434133313183	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	30	2.1661126E-6	45.000004	1
TCGCAAA	30	2.1661126E-6	45.000004	31
CGAACGA	30	2.1661126E-6	45.000004	22
GATCGAA	30	2.1661126E-6	45.000004	13
GTACAAC	30	2.1661126E-6	45.000004	31
TCCGATA	30	2.1661126E-6	45.000004	14
TAGCGCT	30	2.1661126E-6	45.000004	29
GACCGAA	30	2.1661126E-6	45.000004	36
ATTAACG	30	2.1661126E-6	45.000004	1
CGATTCG	65	0.0	45.000004	10
AGTGATC	30	2.1661126E-6	45.000004	10
AGCGCGA	30	2.1661126E-6	45.000004	5
CTACCGG	65	0.0	45.000004	2
TACTCCG	30	2.1661126E-6	45.000004	34
CGTGTAC	30	2.1661126E-6	45.000004	18
TATCGAG	135	0.0	45.000004	1
AATCGTA	30	2.1661126E-6	45.000004	13
ACGCGAG	105	0.0	45.0	1
CGGACAC	20	7.034234E-4	45.0	27
TCACGAC	70	0.0	45.0	25
>>END_MODULE