##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548945_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1921811
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8621487752958	31.0	31.0	33.0	30.0	33.0
2	31.179796036134668	31.0	31.0	33.0	30.0	34.0
3	31.347704326804248	31.0	31.0	34.0	30.0	34.0
4	35.19557490304718	37.0	35.0	37.0	33.0	37.0
5	35.08052508805496	37.0	35.0	37.0	32.0	37.0
6	35.15738696469111	37.0	35.0	37.0	32.0	37.0
7	35.53518738315058	36.0	35.0	37.0	35.0	37.0
8	35.603171175521425	37.0	35.0	37.0	35.0	37.0
9	37.434760754309345	39.0	37.0	39.0	35.0	39.0
10	36.90403322699267	39.0	37.0	39.0	32.0	39.0
11	36.47512476513039	38.0	35.0	39.0	32.0	39.0
12	35.23401728890094	35.0	35.0	39.0	31.0	39.0
13	34.819544689878455	35.0	34.0	39.0	30.0	39.0
14	35.515361292031315	36.0	34.0	40.0	29.0	41.0
15	35.956943736923144	36.0	34.0	40.0	31.0	41.0
16	36.12049363855239	36.0	34.0	40.0	32.0	41.0
17	35.974470954740084	36.0	34.0	40.0	31.0	41.0
18	36.06598151431124	36.0	35.0	40.0	31.0	41.0
19	35.945317203408656	36.0	35.0	40.0	31.0	41.0
20	35.666178932267535	36.0	34.0	40.0	31.0	41.0
21	35.540575530059925	35.0	34.0	40.0	30.0	41.0
22	35.36947025487938	35.0	34.0	40.0	30.0	41.0
23	35.30895493885715	35.0	34.0	40.0	30.0	41.0
24	35.41176837888845	35.0	34.0	40.0	30.0	41.0
25	35.339402782063374	35.0	34.0	40.0	30.0	41.0
26	35.06957395914583	35.0	34.0	39.0	29.0	41.0
27	35.08167556539119	35.0	34.0	39.0	29.0	41.0
28	35.15369773614575	36.0	34.0	40.0	29.0	41.0
29	35.11506282355549	36.0	34.0	40.0	29.0	41.0
30	35.087753166154215	36.0	34.0	39.0	29.0	41.0
31	34.87611476883003	35.0	34.0	40.0	29.0	41.0
32	34.75413503200887	35.0	34.0	40.0	29.0	41.0
33	34.52423261184372	35.0	33.0	40.0	27.0	41.0
34	34.33492731595354	35.0	34.0	40.0	26.0	41.0
35	34.10784879470458	35.0	33.0	40.0	24.0	41.0
36	33.886755773590636	35.0	33.0	40.0	23.0	41.0
37	33.91636534497929	35.0	33.0	40.0	23.0	41.0
38	33.95541861296454	35.0	33.0	40.0	24.0	41.0
39	33.980851915198734	35.0	33.0	40.0	24.0	41.0
40	33.623410938952894	35.0	33.0	40.0	23.0	41.0
41	33.726939329621906	35.0	33.0	39.0	23.0	41.0
42	33.72051466039064	35.0	33.0	39.0	23.0	41.0
43	33.673528770519056	35.0	33.0	39.0	23.0	41.0
44	33.63053963162871	35.0	33.0	39.0	23.0	41.0
45	33.71920287686979	35.0	33.0	39.0	23.0	41.0
46	33.72545635340832	35.0	33.0	39.0	23.0	41.0
47	33.66121382383596	35.0	33.0	39.0	23.0	41.0
48	33.71183794868486	35.0	33.0	39.0	23.0	41.0
49	33.76202134340994	35.0	33.0	39.0	24.0	41.0
50	33.55123214509647	35.0	33.0	39.0	24.0	41.0
51	33.27764801013211	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	2.0
13	9.0
14	20.0
15	57.0
16	153.0
17	441.0
18	1047.0
19	2311.0
20	3989.0
21	6468.0
22	10012.0
23	14424.0
24	20441.0
25	28076.0
26	35451.0
27	40769.0
28	45560.0
29	51602.0
30	61901.0
31	76888.0
32	97872.0
33	129469.0
34	232849.0
35	336001.0
36	111579.0
37	137625.0
38	198547.0
39	278142.0
40	104.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.262011717073115	24.345994481247114	29.492130079388662	12.89986372229111
2	29.774884210778268	27.32537174571277	31.4398242074793	11.45991983602966
3	28.34487886686048	26.616040807342657	32.92306059232672	12.116019733470148
4	24.764922253020718	28.786597641495444	32.76383577781582	13.684644327668016
5	21.312657696308328	33.51281681705433	31.98202112486608	13.192504361771269
6	20.16826836770109	41.041392728004986	29.40034165690591	9.38999724738801
7	87.1340626107354	4.803125801652712	6.82762248733096	1.235189100280933
8	88.73661353795977	3.4394641304477913	6.464475434889279	1.3594468967031619
9	84.67872230932178	4.913698589507501	8.189879233701962	2.2176998674687574
10	56.937857052540544	22.6031071733901	13.689951821485046	6.769083952584307
11	52.367064191015665	17.574568987272944	20.52423469321385	9.534132128497548
12	49.10545313769148	21.577095770603876	21.107902910327812	8.209548181376837
13	17.20502172169896	52.75159732148479	19.90695234859203	10.136428608224222
14	10.166868646292482	51.43148832013138	26.01738672533355	12.38425630824259
15	9.141897928568419	23.35406551424672	56.99967374523302	10.504362811951852
16	9.550210712707962	17.72401136219951	53.94630377284759	18.77947415224494
17	9.88385434363733	18.449993261564224	30.687044667763896	40.97910772703455
18	15.891781241755822	23.40932589104756	44.05240681836039	16.64648604883623
19	31.739697608141487	20.677059294592446	28.93624815343444	18.646994943831626
20	31.57167900485532	21.052902704792512	30.444200808508228	16.931217481843948
21	15.67240483065192	35.137586370355876	30.863232648788042	18.326776150204157
22	18.55250074018725	24.631558462304564	22.57386392314333	34.242076874364855
23	12.872129465384475	33.65492236229265	26.650175277381592	26.82277289494128
24	10.137781498805033	21.26202836803411	56.468612158011375	12.131577975149481
25	9.948428851744525	26.88292449153429	46.79393551186875	16.374711144852434
26	10.256679767157124	41.73178319824374	29.881658498156167	18.12987853644297
27	10.039124554912007	49.50299483143764	26.980072442087177	13.477808171563177
28	9.836763344574466	34.04106855460813	42.70154557342007	13.420622527397336
29	9.886247919280304	25.432469686144994	40.214776583129144	24.466505811445558
30	14.099409359192968	42.197697900573985	30.217071293691212	13.485821446541829
31	31.151554445260228	31.154416329181174	26.357222432382788	11.336806793175812
32	34.534145137060825	22.51568962816843	29.905386117573475	13.044779117197269
33	34.408638518564	27.69408646323702	21.566480782969812	16.330794235229167
34	18.55426990479293	25.855404095407923	25.84411266248346	29.746213337315687
35	18.03403144221778	26.177600190653504	35.080608863202464	20.707759503926244
36	36.415859832210344	27.70860401985419	22.96151910879894	12.914017039136521
37	14.662576080582326	45.59537852577595	28.599170261799937	11.142875131841787
38	15.269191403316976	42.55085437641891	24.880854568945647	17.29909965131847
39	19.24153831984519	40.82669939968082	26.53309820788829	13.398664072585701
40	26.437251113663102	24.433359992215674	27.520864434640036	21.60852445948119
41	12.310471737335254	25.350827943018327	28.87833402972509	33.460366289921325
42	16.040339034379553	21.7173280827303	27.042617614323156	35.199715268567
43	16.328348625333085	22.196251348337583	31.369421863023994	30.10597816330534
44	11.59812281228487	31.882635701429535	35.66521369687238	20.854027789413216
45	10.773744140292672	53.33781521700105	21.798085243554127	14.090355399152156
46	16.845985375252823	42.460939187048055	24.758574074141528	15.934501363557601
47	16.79717724583739	30.000296595242716	26.914561317424035	26.28796484149586
48	16.961709554165317	27.37522056019036	40.29131896945121	15.371750916193111
49	20.77597640975101	28.30371977265194	38.05296150349852	12.867342314098526
50	17.492719107133844	44.438813181941406	24.97623335489286	13.09223435603189
51	15.9485506118968	37.87708572799302	25.89214027810227	20.282223382007906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2542.0
1	3191.5
2	3841.0
3	8971.0
4	14101.0
5	11414.5
6	8728.0
7	9155.5
8	9583.0
9	10057.5
10	10532.0
11	10630.5
12	10729.0
13	10297.0
14	9865.0
15	9145.5
16	8426.0
17	8005.0
18	7584.0
19	7441.0
20	7298.0
21	8956.5
22	10615.0
23	10125.5
24	9636.0
25	13602.0
26	21670.0
27	25772.0
28	33970.0
29	42168.0
30	48602.5
31	55037.0
32	63334.0
33	71631.0
34	81239.0
35	90847.0
36	92992.0
37	95137.0
38	113630.0
39	132123.0
40	172115.0
41	212107.0
42	245970.5
43	279834.0
44	271367.0
45	262900.0
46	220340.5
47	177781.0
48	155000.0
49	132219.0
50	110981.0
51	89743.0
52	67762.5
53	45782.0
54	33574.0
55	21366.0
56	17533.0
57	13700.0
58	12698.0
59	11696.0
60	10808.0
61	9920.0
62	8467.0
63	7014.0
64	5801.0
65	4588.0
66	3773.5
67	2959.0
68	2557.0
69	2155.0
70	1881.5
71	1608.0
72	1409.5
73	1211.0
74	959.5
75	541.5
76	375.0
77	282.5
78	190.0
79	140.5
80	91.0
81	75.5
82	60.0
83	36.0
84	12.0
85	10.0
86	8.0
87	9.0
88	10.0
89	9.0
90	8.0
91	5.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1921811.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.070941454712063
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.81117947231093	12.987117341857383
2	7.62590505663327	2.4511094740869193
3	2.7134164330723203	1.3082146991447667
4	1.4072273641130366	0.9046187432851756
5	0.9351451728228476	0.751433166204629
6	0.6337276386861675	0.6110759867734992
7	0.4915320807341514	0.5529568304813945
8	0.4011530123861283	0.5157525261151081
9	0.3319799223679942	0.4801706906864301
>10	2.825216804669008	9.508317436264306
>50	0.49871194314840855	5.733329881918459
>100	1.1980139362013804	50.327791230200525
>500	0.11736310202678257	11.435676362082075
>1k	0.009102955281855712	2.082847461676308
>5k	3.2510554578056117E-4	0.3495881692230322
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6691	0.34816118754653813	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2986	0.15537427978089416	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2635	0.13711025693993845	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2187	0.11379891154749348	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2051	0.10672225312478698	No Hit
ATTGTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGC	1934	0.10063424551113506	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024091859189066978	0.0
2	0.0	0.0	0.0	0.08981112086464278	0.0
3	0.0	0.0	0.0	0.12483017320641832	0.0
4	0.0	0.0	0.0	0.19309911328429277	0.0
5	0.0	0.0	0.0	0.3497222151397822	0.0
6	0.0	0.0	0.0	0.4866243350672881	0.0
7	0.0	0.0	0.0	0.5913692865739659	0.0
8	0.0	0.0	0.0	0.8860392619253402	0.0
9	0.0	0.0	0.0	1.0025959888875648	0.0
10	0.0	0.0	0.0	1.231754839575796	0.0
11	0.0	0.0	0.0	1.3856201260165542	0.0
12	0.0	0.0	0.0	1.5426595018969087	0.0
13	0.0	0.0	0.0	1.602446858718157	0.0
14	0.0	0.0	0.0	1.6199824020155988	0.0
15	0.0	0.0	0.0	1.6554177283822396	0.0
16	0.0	0.0	0.0	1.730971463895253	0.0
17	0.0	0.0	0.0	1.8215630985565179	0.0
18	0.0	0.0	0.0	1.975012110972411	0.0
19	0.0	0.0	0.0	2.0329782689348743	0.0
20	0.0	0.0	0.0	2.090944426897338	0.0
21	0.0	0.0	0.0	2.1740951633641394	0.0
22	0.0	0.0	0.0	2.26005574949878	0.0
23	0.0	0.0	0.0	2.3695878522914064	0.0
24	0.0	0.0	0.0	2.454091479339019	0.0
25	0.0	0.0	0.0	2.516272411803242	0.0
26	0.0	0.0	0.0	2.5846454203873326	0.0
27	0.0	0.0	0.0	2.6513012986188547	0.0
28	0.0	0.0	0.0	2.7243573899826776	0.0
29	0.0	0.0	0.0	2.8080284689805604	0.0
30	0.0	0.0	0.0	2.9300487925191394	0.0
31	0.0	0.0	0.0	3.0385922445027114	0.0
32	0.0	0.0	0.0	3.1348035784996546	0.0
33	0.0	0.0	0.0	3.226331829716866	0.0
34	0.0	0.0	0.0	3.323896054294621	0.0
35	0.0	0.0	0.0	3.471152990590646	0.0
36	0.0	0.0	0.0	3.576418284628405	0.0
37	0.0	0.0	0.0	3.6877715862798164	0.0
38	0.0	0.0	0.0	3.7931929830769	0.0
39	0.0	0.0	0.0	3.909125299001827	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	125	0.0	45.000004	1
TATTGCG	75	0.0	45.000004	1
TTGCGAG	75	0.0	45.000004	1
TTACCCG	75	0.0	45.000004	45
TATCCGA	75	0.0	45.000004	30
AACGTGC	20	7.034472E-4	45.0	44
CGGGTGC	50	2.1827873E-11	45.0	6
AACGTAG	20	7.034472E-4	45.0	1
TACCCGG	35	1.212502E-7	45.0	2
TACCCGC	40	6.8193913E-9	45.0	45
CGAACGG	100	0.0	45.0	2
CACGGTA	20	7.034472E-4	45.0	30
GTCGAAG	45	3.8562575E-10	45.0	1
ACGTTAC	20	7.034472E-4	45.0	45
GCGAAAG	70	0.0	45.0	1
TCCGAAC	50	2.1827873E-11	45.0	39
GCAACCG	20	7.034472E-4	45.0	30
ATTACGT	55	1.8189894E-12	45.0	31
ATTACGC	20	7.034472E-4	45.0	33
TTACTCG	25	3.8917886E-5	45.0	20
>>END_MODULE