##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1928597
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92554276502556	31.0	31.0	33.0	30.0	33.0
2	31.252946572041747	31.0	31.0	33.0	30.0	34.0
3	31.381366869283735	31.0	31.0	34.0	30.0	34.0
4	35.22702150838148	37.0	35.0	37.0	33.0	37.0
5	35.11759792222014	37.0	35.0	37.0	32.0	37.0
6	35.207435249562245	37.0	35.0	37.0	32.0	37.0
7	35.57558992365953	36.0	35.0	37.0	35.0	37.0
8	35.64633617080188	37.0	35.0	37.0	35.0	37.0
9	37.44813976170242	39.0	37.0	39.0	35.0	39.0
10	36.975969577884854	39.0	37.0	39.0	33.0	39.0
11	36.58323952593518	39.0	35.0	39.0	32.0	39.0
12	35.36479471864781	35.0	35.0	39.0	31.0	39.0
13	34.92811613831194	35.0	34.0	39.0	30.0	39.0
14	35.62697909412905	36.0	34.0	40.0	30.0	41.0
15	36.01199213728944	36.0	34.0	40.0	31.0	41.0
16	36.194763343508264	36.0	35.0	40.0	32.0	41.0
17	36.04713426392346	36.0	34.0	40.0	31.0	41.0
18	36.128907179675174	36.0	35.0	40.0	31.0	41.0
19	35.98527530634964	36.0	35.0	40.0	31.0	41.0
20	35.719032540235204	36.0	34.0	40.0	31.0	41.0
21	35.6022180891083	35.0	34.0	40.0	30.0	41.0
22	35.40666453385544	35.0	34.0	40.0	30.0	41.0
23	35.32080470933015	35.0	34.0	40.0	30.0	41.0
24	35.383483433812245	35.0	34.0	40.0	30.0	41.0
25	35.30268272739198	35.0	34.0	40.0	30.0	41.0
26	35.060677269538424	35.0	34.0	39.0	29.0	41.0
27	35.05418550376258	35.0	34.0	39.0	29.0	41.0
28	35.117423183796305	36.0	34.0	39.0	29.0	41.0
29	35.09806040349539	36.0	34.0	39.0	29.0	41.0
30	35.074969524478156	36.0	34.0	39.0	29.0	41.0
31	34.84031811726348	35.0	34.0	40.0	29.0	41.0
32	34.68289020464099	35.0	34.0	40.0	28.0	41.0
33	34.41413628663739	35.0	33.0	40.0	27.0	41.0
34	34.17770327341586	35.0	34.0	40.0	25.0	41.0
35	33.94477539890397	35.0	33.0	40.0	24.0	41.0
36	33.70951577753154	35.0	33.0	40.0	23.0	41.0
37	33.71294002842481	35.0	33.0	40.0	23.0	41.0
38	33.73028424289782	35.0	33.0	40.0	23.0	41.0
39	33.74318740514477	35.0	33.0	40.0	23.0	41.0
40	33.44041393821519	35.0	32.0	39.0	23.0	41.0
41	33.505252263692206	35.0	33.0	39.0	23.0	41.0
42	33.4851184565775	35.0	33.0	39.0	23.0	41.0
43	33.45932872445617	35.0	33.0	39.0	23.0	41.0
44	33.40599824639362	35.0	33.0	39.0	23.0	41.0
45	33.455744253465085	35.0	33.0	39.0	23.0	41.0
46	33.45749060068018	35.0	33.0	39.0	23.0	41.0
47	33.40845132497873	35.0	33.0	39.0	23.0	41.0
48	33.464667838848655	35.0	33.0	39.0	23.0	41.0
49	33.48806723229374	35.0	33.0	39.0	23.0	41.0
50	33.29995068954271	35.0	32.0	39.0	23.0	41.0
51	33.01619363713622	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	3.0
13	7.0
14	16.0
15	48.0
16	144.0
17	397.0
18	1145.0
19	2200.0
20	4084.0
21	6531.0
22	9877.0
23	14808.0
24	21229.0
25	29656.0
26	38400.0
27	44248.0
28	48098.0
29	53205.0
30	62086.0
31	76450.0
32	96966.0
33	128588.0
34	233937.0
35	334866.0
36	113530.0
37	136045.0
38	195738.0
39	276187.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.79067166442756	25.58927551997644	30.253495157360504	14.366557658235493
2	30.81348773227377	27.093840755741088	30.598979465383387	11.493692046601753
3	29.274026662905726	24.80191558941552	33.731826815037046	12.192230932641708
4	26.122824001074356	27.317941488035085	33.578451070908024	12.980783439982538
5	22.323274380287845	32.83837940222867	32.06968589083152	12.768660326651965
6	20.064585810306664	41.58152273388375	29.516534558541778	8.837356897267806
7	84.86723768625586	5.423268832213262	8.379303711454494	1.330189770076382
8	86.97379494005227	3.638085095019851	8.081781730449649	1.3063382344782242
9	81.94770602671268	5.734738776426594	9.949408818949735	2.368146377910989
10	56.32669759415783	21.414997534477134	14.7034346729773	7.554870198387739
11	52.9003208031538	16.686897262621482	21.22428895202056	9.188492982204162
12	49.14183730452759	19.72392366056776	22.117425257842875	9.01681377706177
13	18.593568277872464	49.57075013597968	21.345361420763385	10.490320165384475
14	10.699902571662198	50.17922354955442	26.656061375186212	12.464812503597175
15	10.081836692683853	23.425993092387888	55.41069492485988	11.081475290068376
16	10.676932505857884	17.32290364446279	54.111408448732426	17.888755400946906
17	10.3734476409535	18.318497851028496	32.10603355703654	39.20202095098146
18	15.642977770887335	23.394882393781593	43.864218392956126	17.09792144237495
19	31.699416726252295	20.27774594692411	28.896135377167965	19.12670194965563
20	31.00989994280817	21.243733138649496	30.740481292877675	17.005885625664668
21	16.087705207464285	33.913150336747385	32.29855693024515	17.70058752554318
22	19.544829738924204	25.63267494453222	22.848111865776	31.97438345076758
23	13.767469305406987	33.39552016310302	26.84365888778216	25.993351643707836
24	10.91109236403458	21.25996255308911	54.377975284624	13.450969798252304
25	10.360121891717139	25.952648479697938	47.36692009787426	16.320309530710666
26	9.974504782492142	39.79488716408871	30.288961353771683	19.941646699647464
27	10.546889785683582	47.883253992410026	27.562782686066605	14.007073535839783
28	9.891491068377686	34.8822485983334	42.7164410190413	12.509819314247611
29	9.771922283400833	25.72916996137607	40.68009024176643	23.818817513456672
30	13.388955805697094	42.06016083194156	30.513736151202142	14.037147211159201
31	30.1947996393233	30.760184735328323	26.99247172944892	12.052543895899454
32	33.15104192322191	22.78345346383926	31.268533550555144	12.796971062383692
33	33.13667915069867	27.88374139335486	22.223253484268614	16.756325971677857
34	18.758766087471876	26.531203771446286	26.695623813580543	28.014406327501284
35	19.300247796714398	24.15543527237676	35.269473093653055	21.274843837255787
36	35.573683874858254	27.50906487980641	23.78589202409835	13.131359221236993
37	15.657392394574918	43.499186195975625	29.313848357121785	11.529573052327677
38	15.728584043218982	41.31225963744629	25.341426954412977	17.617729364921754
39	19.75145662883433	39.81811648571475	27.368703777927685	13.06172310752324
40	27.169802711504786	24.928069472264035	26.040173245110303	21.861954571120872
41	13.305942091582637	24.588807303962415	30.037068397389397	32.06818220706555
42	15.309989593471315	22.59295228604006	28.08357578073594	34.013482339752684
43	16.81284374081262	22.91971832373482	30.37529354240414	29.89214439304842
44	12.917887977633482	30.28678360486924	34.89827060811564	21.89705780938164
45	11.824346921622299	50.89741402688068	22.404369601321584	14.87386945017544
46	17.899436740801733	41.66889194580309	24.96638748271412	15.46528383068106
47	17.182698096077097	29.979098795653	27.211387345308534	25.626815762961364
48	17.53445639498558	28.070250031499583	38.96770553931174	15.427588034203103
49	21.45590810314441	28.21066298454265	37.43068147466786	12.902747437645088
50	18.18705514941691	41.996020941648254	25.63272679569656	14.184197113238275
51	16.10419387772562	37.599094056456586	25.801813442621764	20.494898623196033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2855.0
1	4350.5
2	5846.0
3	13788.0
4	21730.0
5	17040.0
6	12350.0
7	12371.5
8	12393.0
9	12847.0
10	13301.0
11	13077.0
12	12853.0
13	12355.0
14	11857.0
15	10875.0
16	9893.0
17	8976.5
18	8060.0
19	8279.5
20	8499.0
21	8737.5
22	8976.0
23	10022.5
24	11069.0
25	12883.5
26	20396.0
27	26094.0
28	33937.5
29	41781.0
30	45309.5
31	48838.0
32	63879.5
33	78921.0
34	78791.0
35	78661.0
36	82119.5
37	85578.0
38	100922.5
39	116267.0
40	163118.5
41	209970.0
42	238258.5
43	266547.0
44	256682.5
45	246818.0
46	211008.0
47	175198.0
48	158383.5
49	141569.0
50	113728.5
51	85888.0
52	68019.5
53	50151.0
54	38553.0
55	26955.0
56	23260.0
57	19565.0
58	18739.0
59	17913.0
60	16669.0
61	15425.0
62	13595.5
63	11766.0
64	10459.5
65	9153.0
66	7881.5
67	6610.0
68	5633.0
69	4656.0
70	4219.5
71	3783.0
72	3262.0
73	2741.0
74	2264.5
75	1325.5
76	863.0
77	603.0
78	343.0
79	270.0
80	197.0
81	146.0
82	95.0
83	69.5
84	44.0
85	28.0
86	12.0
87	13.5
88	15.0
89	12.0
90	9.0
91	5.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1928597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.516465878438744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.93044990504727	14.000989984100176
2	7.653320808815637	2.6811826560872865
3	2.8631524451025787	1.5045693632822332
4	1.6299963539428548	1.1420710206327833
5	1.061715791872317	0.9298754220465505
6	0.774932653198001	0.8144448826698472
7	0.5862281405298472	0.7188051654400163
8	0.4698975830066625	0.6584755983277634
9	0.38843642559381325	0.6123630055371065
>10	3.077946302530178	10.967288066034266
>50	0.4495948175980986	5.58111268760655
>100	0.9705060724916368	43.54019362087777
>500	0.13639384178609468	14.453400491881613
>1k	0.0071317041456781536	1.8670671628425493
>5k	0.0	0.0
>10k+	2.971543394032564E-4	0.5281608726334917
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10147	0.5261337645967509	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3321	0.1721977167858293	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3162	0.16395338165516177	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2224	0.11531698950065775	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2083	0.10800597532817897	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012651684099892304	0.0
2	0.0	0.0	0.0	0.053095592288072625	0.0
3	0.0	0.0	0.0	0.08581367698902363	0.0
4	0.0	0.0	0.0	0.15052393009011214	0.0
5	0.0	0.0	0.0	0.28678878998567353	0.0
6	0.0	0.0	0.0	0.44514224589170265	0.0
7	0.0	0.0	0.0	0.5567259515596052	0.0
8	0.0	0.0	0.0	0.867469979472124	0.0
9	0.0	0.0	0.0	1.0137939652503867	0.0
10	0.0	0.0	0.0	1.2493019537000214	0.0
11	0.0	0.0	0.0	1.4354994848586822	0.0
12	0.0	0.0	0.0	1.6066083271932914	0.0
13	0.0	0.0	0.0	1.6689334267345641	0.0
14	0.0	0.0	0.0	1.6899849994581553	0.0
15	0.0	0.0	0.0	1.724258619089421	0.0
16	0.0	0.0	0.0	1.8077908448473166	0.0
17	0.0	0.0	0.0	1.8996711080645672	0.0
18	0.0	0.0	0.0	2.0611356338312254	0.0
19	5.185116434382092E-5	0.0	0.0	2.1248607148097816	0.0
20	5.185116434382092E-5	0.0	0.0	2.2091188568684905	0.0
21	5.185116434382092E-5	0.0	0.0	2.309710115695503	0.0
22	5.185116434382092E-5	0.0	0.0	2.4004496532971897	0.0
23	5.185116434382092E-5	0.0	0.0	2.5104778240347776	0.0
24	5.185116434382092E-5	0.0	0.0	2.592039705547608	0.0
25	5.185116434382092E-5	0.0	0.0	2.6605869448101394	0.0
26	5.185116434382092E-5	0.0	0.0	2.733230426055832	0.0
27	5.185116434382092E-5	0.0	0.0	2.8057183538084938	0.0
28	5.185116434382092E-5	0.0	0.0	2.8840136119676636	0.0
29	5.185116434382092E-5	0.0	0.0	2.9726791029955972	0.0
30	5.185116434382092E-5	0.0	0.0	3.0994033486518955	0.0
31	5.185116434382092E-5	0.0	0.0	3.2072019193226993	0.0
32	5.185116434382092E-5	0.0	0.0	3.3127708899267185	0.0
33	5.185116434382092E-5	0.0	0.0	3.414243618547576	0.0
34	5.185116434382092E-5	0.0	0.0	3.5265532405162925	0.0
35	5.185116434382092E-5	0.0	0.0	3.675780891497809	0.0
36	5.185116434382092E-5	0.0	0.0	3.7972163183910377	0.0
37	5.185116434382092E-5	0.0	0.0	3.9147629079584796	0.0
38	5.185116434382092E-5	0.0	0.0	4.032413199854609	0.0
39	5.185116434382092E-5	0.0	0.0	4.148352403327393	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTG	20	7.034476E-4	45.000004	1
TATCACG	35	1.212502E-7	45.000004	1
GGTAATC	125	0.0	45.000004	8
ACGCATG	40	6.8193913E-9	45.000004	1
CAGTTCG	20	7.034476E-4	45.000004	1
GTTAGTC	20	7.034476E-4	45.000004	35
TACGGCA	20	7.034476E-4	45.000004	36
ATCCGGT	20	7.034476E-4	45.000004	11
TGTTGCG	20	7.034476E-4	45.000004	1
GTTACGT	35	1.212502E-7	45.000004	29
TGATTCG	35	1.212502E-7	45.000004	15
CTCATCG	20	7.034476E-4	45.000004	21
CGTGTTA	20	7.034476E-4	45.000004	32
ATACGTT	20	7.034476E-4	45.000004	27
GATTCGT	20	7.034476E-4	45.000004	11
TGCGTAG	20	7.034476E-4	45.000004	1
AGTCGAT	20	7.034476E-4	45.000004	24
ATAGCCG	25	3.891794E-5	45.0	15
TCGATTG	95	0.0	45.0	1
CACGAAC	25	3.891794E-5	45.0	42
>>END_MODULE