##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548941_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2091997
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.037600436329498	31.0	31.0	33.0	30.0	33.0
2	31.363978533430018	31.0	31.0	33.0	30.0	34.0
3	31.549228799085277	31.0	31.0	34.0	30.0	34.0
4	35.381577507042316	37.0	35.0	37.0	33.0	37.0
5	35.278609386151125	37.0	35.0	37.0	33.0	37.0
6	35.35064199422848	37.0	35.0	37.0	33.0	37.0
7	35.6557036171658	36.0	35.0	37.0	35.0	37.0
8	35.72968364677387	37.0	35.0	37.0	35.0	37.0
9	37.62592250371296	39.0	37.0	39.0	35.0	39.0
10	37.18320580765651	39.0	37.0	39.0	33.0	39.0
11	36.74740116740129	39.0	35.0	39.0	33.0	39.0
12	35.040392027330824	35.0	35.0	39.0	31.0	39.0
13	34.47855661360891	35.0	33.0	39.0	29.0	39.0
14	35.045246718805046	35.0	33.0	40.0	27.0	41.0
15	35.55964038189347	35.0	34.0	40.0	31.0	41.0
16	35.834128347220386	35.0	34.0	40.0	32.0	41.0
17	35.745872484520774	35.0	34.0	40.0	32.0	41.0
18	35.89433732457551	36.0	35.0	39.0	31.0	41.0
19	35.75097144020761	36.0	35.0	39.0	31.0	41.0
20	35.43215597345503	35.0	34.0	39.0	31.0	41.0
21	35.265584988888605	35.0	34.0	39.0	31.0	41.0
22	35.10609288636647	35.0	34.0	39.0	30.0	41.0
23	35.108876829173276	35.0	34.0	39.0	30.0	41.0
24	35.18257244154748	35.0	34.0	39.0	31.0	41.0
25	35.0839408469515	35.0	34.0	39.0	31.0	41.0
26	34.8841929505635	35.0	34.0	38.0	30.0	41.0
27	34.85827226329675	35.0	34.0	39.0	30.0	41.0
28	35.03179211060054	35.0	34.0	39.0	30.0	41.0
29	35.06379406853834	35.0	34.0	39.0	30.0	41.0
30	35.029753388747686	35.0	34.0	38.0	30.0	41.0
31	34.74858950562549	35.0	34.0	38.0	29.0	41.0
32	34.59100801769792	35.0	34.0	39.0	29.0	41.0
33	34.35691781584773	35.0	33.0	39.0	29.0	41.0
34	34.17583916229325	35.0	34.0	39.0	27.0	41.0
35	33.947041033041636	35.0	33.0	38.0	27.0	41.0
36	33.641930174852064	35.0	33.0	38.0	23.0	41.0
37	33.63810559957782	35.0	33.0	38.0	24.0	41.0
38	33.747376788781246	35.0	33.0	38.0	26.0	41.0
39	33.780390220444865	35.0	33.0	38.0	25.0	41.0
40	33.373641071187	35.0	33.0	38.0	23.0	41.0
41	33.5496336753829	35.0	33.0	38.0	23.0	41.0
42	33.55854047591846	35.0	33.0	38.0	23.0	41.0
43	33.54813606329263	35.0	33.0	38.0	24.0	41.0
44	33.51337262911945	35.0	33.0	38.0	23.0	41.0
45	33.57190521783731	35.0	33.0	38.0	24.0	41.0
46	33.60806588154763	35.0	33.0	38.0	24.0	41.0
47	33.585721203233085	35.0	33.0	38.0	24.0	41.0
48	33.66698613812544	35.0	33.0	38.0	24.0	41.0
49	33.72073047905901	35.0	33.0	38.0	24.0	41.0
50	33.428265910515165	35.0	33.0	38.0	24.0	40.0
51	33.136960043441746	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	8.0
14	19.0
15	30.0
16	118.0
17	335.0
18	843.0
19	1879.0
20	3381.0
21	5704.0
22	8986.0
23	13632.0
24	20148.0
25	28087.0
26	36527.0
27	42387.0
28	46937.0
29	53779.0
30	65231.0
31	81984.0
32	109627.0
33	152180.0
34	305014.0
35	459234.0
36	97916.0
37	118712.0
38	175316.0
39	263800.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.89684163027002	25.018630523848746	30.221506053784974	13.86302179209626
2	28.206302399095218	27.333117590512796	32.21830624040092	12.242273769991066
3	27.234981694524418	26.671214155660834	34.34742019228517	11.746383957529575
4	25.145925161460557	28.20706721854764	34.36372040686483	12.283287213126979
5	22.981151502607318	32.06778021192191	33.084942282422006	11.866126003048763
6	19.685353277275254	41.0635388100461	30.612328793970544	8.638779118708104
7	87.43277356516286	5.327206492169922	6.350773925584023	0.8892460170831985
8	90.65242445376356	2.6713709436485806	5.707704169747854	0.9685004328400089
9	87.63822319056864	3.6980454560881304	7.173528451522635	1.4902029018206049
10	67.5195996934986	16.165749759679386	10.98395456590043	5.330695980921578
11	65.39942456896448	12.9989192145113	15.135537957272405	6.466118259251806
12	59.42135672278689	18.4208677163495	15.916896630348894	6.240878930514718
13	16.575597383743858	61.09114879227838	14.732095696121936	7.6011581278558245
14	8.21554715422632	59.380391080866744	23.45098965247082	8.953072112436107
15	7.138681365221842	20.49644430656449	64.40214780422725	7.962726523986412
16	6.422475749248206	13.610009956993247	63.1292492293249	16.83826506443365
17	7.204025627187802	14.005182607814447	29.967633796797987	48.823157968199766
18	13.952792475323816	21.079093325659645	48.050594718826076	16.91751948019046
19	35.42791887368863	17.00662094639715	28.181541369323188	19.383918810591027
20	35.57261315384295	17.842042794516434	29.35338817407482	17.23195587756579
21	14.869093980536302	35.66558651852751	32.50009440740116	16.96522509353503
22	18.772541260814428	23.542528980682096	17.894671933085945	39.79025782541753
23	11.631326431156449	35.02218215418091	23.34831264098371	29.99817877367893
24	8.766695172125008	18.275074008232327	61.473845325782015	11.484385493860652
25	7.745326594636608	23.246543852596346	52.71575437249672	16.292375180270334
26	8.08318558774224	44.43978648152937	29.027957497070982	18.44907043365741
27	7.061912612685391	56.058445590505144	25.017626698317446	11.862015098492016
28	6.691596594067774	37.273523814804705	44.46282666753346	11.57205292359406
29	7.31535465873039	23.88650652940707	43.3034559800994	25.494682831763143
30	11.595188712029701	47.06369081791226	28.794113949494193	12.547006520563844
31	34.02915969764775	31.573850249307238	25.240093556539517	9.156896496505492
32	38.85947255182488	20.186357819824792	30.514288500413723	10.439881127936609
33	39.777877310531515	27.90835742116265	17.778849587260403	14.534915681045431
34	19.252274262343587	26.22384257721211	21.94706780172247	32.57681535872183
35	18.30657500942879	23.064277816841994	37.26458498745457	21.364562186274647
36	43.585626556825844	24.005674960336943	21.253041949868955	11.15565653296826
37	14.791225800036997	48.37597759461414	27.55137794174657	9.28141866360229
38	15.611494662755252	44.6615363215148	22.186504091545064	17.54046492418488
39	19.319195964430158	42.514210106419846	25.84688218960161	12.319711739548383
40	30.378102836667548	22.013176883140844	25.013324588897596	22.595395691294012
41	11.74571474050871	23.125272168172327	27.783787452850078	37.34522563846889
42	15.035776819947639	18.273783375406367	26.97981880471148	39.71062099993451
43	16.612738928401903	18.643525779434675	29.592824463897415	35.15091082826601
44	10.301209800970078	30.746554607869896	36.857175225394684	22.09506036576534
45	9.799631643831228	58.32059032589435	19.017761497745933	12.86201653252849
46	17.80365841824821	45.41794275995616	23.04855121685165	13.729847604943984
47	17.17526363565531	29.88417287405288	24.349795912709244	28.59076757758257
48	17.868094457114424	26.704149193330583	41.68987814036062	13.737878209194374
49	23.514947679179272	26.539952017139605	39.21850748351934	10.72659282016179
50	19.343287777181324	46.779034577965454	22.426179387446542	11.45149825740668
51	16.046820334828396	41.47625450705713	22.91800609656706	19.55891906154741
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3129.0
1	3607.5
2	4086.0
3	8468.0
4	12850.0
5	10584.5
6	8319.0
7	9148.5
8	9978.0
9	10795.5
10	11613.0
11	11819.0
12	12025.0
13	11577.5
14	11130.0
15	10333.5
16	9537.0
17	8760.5
18	7984.0
19	8226.5
20	8469.0
21	8245.5
22	8022.0
23	7200.5
24	6379.0
25	10008.0
26	17791.5
27	21946.0
28	25021.5
29	28097.0
30	36141.0
31	44185.0
32	56744.5
33	69304.0
34	69257.5
35	69211.0
36	78004.5
37	86798.0
38	107074.5
39	127351.0
40	181041.0
41	234731.0
42	294267.5
43	353804.0
44	351310.5
45	348817.0
46	283521.5
47	218226.0
48	180692.0
49	143158.0
50	119092.5
51	95027.0
52	72484.0
53	49941.0
54	35751.0
55	21561.0
56	17118.0
57	12675.0
58	11091.5
59	9508.0
60	9260.0
61	9012.0
62	8045.0
63	7078.0
64	5864.0
65	4650.0
66	3776.5
67	2903.0
68	2540.0
69	2177.0
70	1918.0
71	1659.0
72	1433.5
73	1208.0
74	1030.5
75	661.0
76	469.0
77	390.0
78	311.0
79	192.0
80	73.0
81	57.5
82	42.0
83	44.5
84	47.0
85	26.0
86	5.0
87	5.0
88	5.0
89	5.0
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2091997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.74597518737138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.24205795548588	10.355092548716106
2	7.9173521843692765	2.0182874898330283
3	2.650802143898	1.0136117505826427
4	1.3085197397986075	0.6671344054263482
5	0.8418881388424209	0.5365342664113883
6	0.5808772610643341	0.44423082938605496
7	0.41717346701550934	0.37220978615865624
8	0.3573967123974823	0.3644295702613131
9	0.28300414468052104	0.32464474254190634
>10	2.6840584913786505	7.396822294157294
>50	0.42356984289278077	3.766162358736308
>100	0.8087360187025118	27.385407282718766
>500	0.449548792518994	38.75938171574253
>1k	0.0346386004285992	6.290406289634705
>5k	3.7650652639781746E-4	0.3056446696929595
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6369	0.30444594327812136	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2967	0.14182620720775413	No Hit
GGCATTGGTATCTTTTCCCCACTTCCAAGCATTTTTTCAACTAATCTTATG	2744	0.1311665360896789	No Hit
TAATTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2596	0.12409195615481283	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2574	0.12304032940773815	No Hit
AATATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2452	0.11720858108305128	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2413	0.1153443336677825	No Hit
TAGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2373	0.11343228503673762	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2331	0.11142463397414051	No Hit
TGGATTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2254	0.10774394035937909	No Hit
TTTTGAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2176	0.10401544552884158	No Hit
TGATTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2106	0.10066936042451304	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02151054709925492	0.0
2	0.0	0.0	0.0	0.083030711803124	0.0
3	0.0	0.0	0.0	0.1094647841273195	0.0
4	0.0	0.0	0.0	0.15755280719809828	0.0
5	0.0	0.0	0.0	0.25568870318647685	0.0
6	0.0	0.0	0.0	0.3459373985717953	0.0
7	0.0	0.0	0.0	0.40176921859830583	0.0
8	0.0	0.0	0.0	0.5335093692772982	0.0
9	0.0	0.0	0.0	0.5871901345938833	0.0
10	0.0	0.0	0.0	0.6945038640112773	0.0
11	0.0	0.0	0.0	0.8061675040642984	0.0
12	0.0	0.0	0.0	0.907075870567692	0.0
13	0.0	0.0	0.0	0.9498101574715452	0.0
14	0.0	0.0	0.0	0.964246124635934	0.0
15	0.0	0.0	0.0	0.9918274261387564	0.0
16	0.0	0.0	0.0	1.0534909944899538	0.0
17	0.0	0.0	0.0	1.1274394752956147	0.0
18	0.0	0.0	0.0	1.2562637518122637	0.0
19	0.0	0.0	0.0	1.3012924970733706	0.0
20	0.0	0.0	0.0	1.3487591043390597	0.0
21	0.0	0.0	0.0	1.416206619799168	0.0
22	0.0	0.0	0.0	1.478539405171231	0.0
23	0.0	0.0	0.0	1.5632909607422956	0.0
24	0.0	0.0	0.0	1.6258627521932394	0.0
25	0.0	0.0	0.0	1.6694574609810626	0.0
26	0.0	0.0	0.0	1.7179756949938265	0.0
27	0.0	0.0	0.0	1.766685133869695	0.0
28	0.0	0.0	0.0	1.817306621376608	0.0
29	0.0	0.0	0.0	1.8707005793985365	0.0
30	0.0	0.0	0.0	1.9608536723523027	0.0
31	0.0	0.0	0.0	2.041589925798173	0.0
32	0.0	0.0	0.0	2.107890212079654	0.0
33	0.0	0.0	0.0	2.1754811311870905	0.0
34	0.0	0.0	0.0	2.24723075606705	0.0
35	0.0	0.0	0.0	2.3610454508299963	0.0
36	0.0	0.0	0.0	2.4366669741878213	0.0
37	0.0	0.0	0.0	2.5200800957171543	0.0
38	0.0	0.0	0.0	2.595510414211875	0.0
39	0.0	0.0	0.0	2.6740000105162673	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCA	25	3.891906E-5	45.000004	24
TCACGAG	65	0.0	45.000004	1
TCGCCTA	165	0.0	45.000004	43
CAGGTAC	50	2.1827873E-11	45.000004	20
GTGCAAC	50	2.1827873E-11	45.000004	20
TCGTTGC	65	0.0	45.000004	24
AATCCGA	65	0.0	45.000004	30
ATCTCGG	75	0.0	45.000004	2
ATCTCGA	75	0.0	45.000004	12
GGTACCC	50	2.1827873E-11	45.000004	8
ATGATCC	25	3.891906E-5	45.000004	17
CAACGCT	25	3.891906E-5	45.000004	18
TTAGCGA	25	3.891906E-5	45.000004	27
TCCGACA	25	3.891906E-5	45.000004	18
CGTGATA	25	3.891906E-5	45.000004	42
TTGTACG	95	0.0	45.000004	1
GCGTTAG	165	0.0	45.000004	1
ATCCGCG	50	2.1827873E-11	45.000004	1
ATCCGAT	65	0.0	45.000004	31
TCCAGCG	25	3.891906E-5	45.000004	12
>>END_MODULE