FastQCFastQC Report
Sat 18 Jun 2016
SRR3548939_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548939_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762321
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71460.4054879899859333No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG31400.17817412378335162No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21190.12023916187800066No Hit
TAGTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA19260.10928769503399212No Hit
GTAATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA18730.106280297403254No Hit
TATTAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA18310.10389707663927288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC551.8189894E-1245.0000046
GCGACGG1100.045.0000042
ACGCCAA207.0343114E-445.00000444
ACGCATC207.0343114E-445.00000420
GCGAACC207.0343114E-445.00000427
CCTCGAC207.0343114E-445.00000422
ATCGTAG207.0343114E-445.0000041
CGGATCA406.8193913E-945.00000431
TATAGCG850.045.0000041
CGGTCTA551.8189894E-1245.00000431
ACGTAAG406.8193913E-945.0000041
GCGGAAC406.8193913E-945.00000421
ACATCGC207.0343114E-445.00000441
CTCTCGA207.0343114E-445.00000416
CGTTTCA207.0343114E-445.00000444
GTAACGT551.8189894E-1245.0000049
ATCGATC207.0343114E-445.00000429
CCTTCGT351.2124292E-745.07
AGTTTCG253.8916558E-545.036
CAACGGA253.8916558E-545.022