##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762321
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.879938444812268	31.0	31.0	33.0	30.0	33.0
2	31.189712884315625	31.0	31.0	33.0	30.0	34.0
3	31.351520523219097	31.0	31.0	34.0	30.0	34.0
4	35.20423294053694	37.0	35.0	37.0	33.0	37.0
5	35.082231330160624	37.0	35.0	37.0	32.0	37.0
6	35.150860711527585	37.0	35.0	37.0	32.0	37.0
7	35.55678619275376	36.0	35.0	37.0	35.0	37.0
8	35.613898376062025	37.0	35.0	37.0	35.0	37.0
9	37.4290801732488	39.0	37.0	39.0	35.0	39.0
10	36.98608085587132	39.0	37.0	39.0	33.0	39.0
11	36.55926814694939	39.0	35.0	39.0	32.0	39.0
12	34.91365704658799	35.0	34.0	39.0	30.0	39.0
13	34.37916077717964	35.0	33.0	39.0	27.0	39.0
14	34.92788203738139	35.0	33.0	40.0	27.0	41.0
15	35.452002784963696	35.0	33.0	40.0	30.0	41.0
16	35.724702820882236	35.0	34.0	40.0	31.0	41.0
17	35.58324845473668	35.0	34.0	40.0	31.0	41.0
18	35.69985547468367	36.0	35.0	39.0	31.0	41.0
19	35.571627983778214	36.0	34.0	39.0	31.0	41.0
20	35.24692380105554	35.0	34.0	39.0	30.0	41.0
21	35.084270686214374	35.0	34.0	39.0	30.0	41.0
22	34.896754337036214	35.0	34.0	39.0	29.0	41.0
23	34.87300440725611	35.0	34.0	39.0	29.0	41.0
24	35.008531362901536	35.0	34.0	39.0	30.0	41.0
25	34.90196905104121	35.0	34.0	39.0	30.0	41.0
26	34.668345324149236	35.0	34.0	38.0	29.0	41.0
27	34.62829643407756	35.0	34.0	39.0	29.0	41.0
28	34.7518857234295	35.0	34.0	39.0	29.0	41.0
29	34.77030404790047	35.0	34.0	39.0	29.0	41.0
30	34.74568083794042	35.0	34.0	38.0	29.0	41.0
31	34.456074120435495	35.0	34.0	38.0	29.0	41.0
32	34.273451885326224	35.0	34.0	39.0	27.0	41.0
33	34.01666098287429	35.0	33.0	39.0	27.0	41.0
34	33.81243087950492	35.0	33.0	38.0	25.0	41.0
35	33.61370544866684	35.0	33.0	38.0	24.0	41.0
36	33.33035865770197	35.0	33.0	38.0	23.0	41.0
37	33.36150394848612	35.0	33.0	38.0	23.0	41.0
38	33.419032060561044	35.0	33.0	38.0	23.0	41.0
39	33.42529141966759	35.0	33.0	38.0	23.0	41.0
40	33.075639455014155	35.0	32.0	38.0	23.0	41.0
41	33.19879579259397	35.0	32.0	38.0	23.0	41.0
42	33.17871261818931	35.0	32.0	38.0	23.0	41.0
43	33.14339612363469	35.0	32.0	38.0	23.0	41.0
44	33.12878584548445	35.0	32.0	38.0	23.0	41.0
45	33.21675563078463	35.0	33.0	38.0	23.0	41.0
46	33.241142220968825	35.0	33.0	38.0	23.0	41.0
47	33.19114054703996	35.0	33.0	38.0	23.0	41.0
48	33.226562016794894	35.0	33.0	38.0	23.0	41.0
49	33.29292336640147	35.0	33.0	38.0	24.0	40.0
50	33.05205067635238	35.0	32.0	38.0	23.0	40.0
51	32.70934693509298	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	1.0
13	6.0
14	14.0
15	39.0
16	150.0
17	451.0
18	1146.0
19	2276.0
20	3996.0
21	6644.0
22	10017.0
23	14346.0
24	20280.0
25	27754.0
26	35214.0
27	40951.0
28	45321.0
29	50940.0
30	60993.0
31	74647.0
32	95524.0
33	127431.0
34	240868.0
35	354618.0
36	88102.0
37	104499.0
38	148586.0
39	207413.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.639809092668138	25.101726643443506	30.309404472851426	12.949059791036936
2	29.34760466453047	27.524894726897088	30.23314140840403	12.894359200168415
3	27.692798304054712	26.476731537557573	33.54729359747742	12.2831765609103
4	25.581378193870467	28.104414576005166	32.90512908828755	13.409078141836817
5	22.04575670380141	33.22867967867375	31.70591509719285	13.019648520331994
6	19.483340435709497	42.03785802926935	29.2150521953719	9.263749339649246
7	86.62241441825866	4.8777719836511055	7.097401665190393	1.4024119328998519
8	88.62142594907512	3.5204142718608016	6.596982048105879	1.261177730958208
9	84.11691173174466	5.175504349094178	8.742675142610228	1.9649087765509234
10	65.35052354253284	15.71223403681849	12.396039087090264	6.541203333558415
11	63.21493076459964	14.789133194236465	16.227350181947557	5.76858585921634
12	55.46815818457591	20.108027992630173	17.51531077482479	6.908503047969126
13	17.682760405170228	56.726158287848804	18.425871336720153	7.165209970260809
14	9.567099296893131	57.55557585706577	24.963443095781074	7.9138817502600265
15	8.200776135562137	22.37571929290975	61.52420586260959	7.899298708918523
16	8.56506845234211	15.411324043690112	61.04960447046821	14.974003033499573
17	9.33757243998114	15.903175414694598	30.47140674145062	44.287845403873646
18	15.198820192235127	21.201926323297513	47.17307459878195	16.42617888568541
19	36.05631437178584	16.83427706984142	28.629006860838636	18.480401697534106
20	35.25872982277349	19.927697621488935	29.087720114553477	15.725852441184099
21	15.886946816158916	36.01948793664718	31.555772189062036	16.53779305813186
22	18.544748658161595	23.95346818201678	21.3668225028244	36.13496065699722
23	12.229213633611584	35.512713064192056	24.84394159747288	27.414131704723488
24	9.517051660849528	19.874472357760023	57.37910403382812	13.229371947562335
25	8.957448728126147	22.8308009721271	53.22146192435998	14.990288375386775
26	8.099659483147509	44.08459071871697	30.378120671546217	17.43762912658931
27	9.044946976175169	54.097976475341326	26.28113720485655	10.575939343626956
28	8.013523075535048	35.57008059258217	45.55203053246259	10.864365799420197
29	8.530511751264385	25.992540519008738	44.007760220754335	21.469187508972542
30	13.102891017016763	45.44200517385879	29.12318470925558	12.331919099868866
31	35.58721708474222	29.212158284444207	25.24585475631284	9.95476987450073
32	39.12334926497499	21.00814777784524	30.368814761896385	9.49968819528338
33	39.4563759950656	25.990951705166086	19.645853394472404	14.906818905295912
34	19.465012333167454	27.216040664555436	22.94360675495554	30.37534024732157
35	18.015219701745597	23.35737927426388	36.93186428579129	21.695536738199227
36	41.215987325805	24.742597971652156	22.989795843095553	11.051618859447286
37	15.998334015199275	46.26977718588157	27.60450564908436	10.127383149834792
38	16.29118645241134	46.22477970812355	19.978369434399294	17.50566440506582
39	19.890984673053318	41.54861685243495	25.31973459999626	13.240663874515482
40	29.478681806549435	23.307785585032466	23.874027489884078	23.339505118534024
41	13.498108460376967	22.900311577743217	27.000245698712096	36.60133426316772
42	15.637332812807655	19.31481268168512	26.823944105529012	38.22391039997821
43	17.091551425648337	19.30686861247185	29.089649388505272	34.51193057337454
44	10.651181027746931	30.745193412550837	36.53466082512777	22.06896473457446
45	10.196610038693292	55.14863637214786	21.47781249840409	13.176941090754749
46	17.960235394119458	44.50891750140865	23.17563031933456	14.355216785137328
47	17.29951580898145	30.08889980883165	24.118591334949762	28.492993047237135
48	17.97776908974018	27.2985454976704	40.44149732086266	14.282188091726763
49	22.615970643259654	26.4627159297313	39.52253874294184	11.398774684067204
50	19.086988125318825	46.02612123444027	22.98616426859806	11.90072637164285
51	16.90367418875449	41.64905258463129	22.988604232713563	18.458668993900655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2718.0
1	3354.0
2	3990.0
3	9031.5
4	14073.0
5	11110.0
6	8147.0
7	8700.5
8	9254.0
9	9863.0
10	10472.0
11	10497.0
12	10522.0
13	10151.0
14	9780.0
15	9478.0
16	9176.0
17	8675.0
18	8174.0
19	8865.0
20	9556.0
21	8950.5
22	8345.0
23	8529.0
24	8713.0
25	10304.0
26	14351.5
27	16808.0
28	23339.5
29	29871.0
30	37758.5
31	45646.0
32	49991.0
33	54336.0
34	53844.0
35	53352.0
36	54653.5
37	55955.0
38	76177.5
39	96400.0
40	161900.0
41	227400.0
42	262261.0
43	297122.0
44	276994.5
45	256867.0
46	223653.0
47	190439.0
48	152276.5
49	114114.0
50	96648.0
51	79182.0
52	61269.0
53	43356.0
54	31979.5
55	20603.0
56	16314.0
57	12025.0
58	11491.0
59	10957.0
60	10279.5
61	9602.0
62	8546.5
63	7491.0
64	6231.0
65	4971.0
66	4298.0
67	3625.0
68	3095.5
69	2566.0
70	2260.0
71	1954.0
72	1671.0
73	1388.0
74	1068.0
75	564.5
76	381.0
77	264.0
78	147.0
79	121.0
80	95.0
81	62.0
82	29.0
83	34.5
84	40.0
85	31.5
86	23.0
87	14.5
88	6.0
89	6.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1762321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.774992795556928
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.18800527688617	12.807401983285175
2	7.499121796220826	2.3659718461677492
3	2.650553467489143	1.25437385561639
4	1.4342889474498777	0.90503591251075
5	0.928586122815197	0.7324219698731937
6	0.6498472786427942	0.6150801683281413
7	0.510306255101558	0.5635054248428301
8	0.40145421328917924	0.5066349857906222
9	0.3196201966890733	0.4537805670076158
>10	2.8100982300011323	9.237895907518133
>50	0.41621033265160434	4.685310176091918
>100	0.940808768328614	39.59393997790507
>500	0.23195054887364444	22.019937556284503
>1k	0.018787271871385534	3.8514301621655775
>5k	3.6129368983433717E-4	0.407279506612336
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7146	0.4054879899859333	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3140	0.17817412378335162	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2119	0.12023916187800066	No Hit
TAGTATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1926	0.10928769503399212	No Hit
GTAATAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1873	0.106280297403254	No Hit
TATTAAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	1831	0.10389707663927288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024229411100474885	0.0
2	0.0	0.0	0.0	0.08863311507948893	0.0
3	0.0	0.0	0.0	0.12500560340596292	0.0
4	0.0	0.0	0.0	0.1910548645791544	0.0
5	0.0	0.0	0.0	0.32599055450170544	0.0
6	0.0	0.0	0.0	0.4675084732009662	0.0
7	0.0	0.0	0.0	0.5744129474709772	0.0
8	0.0	0.0	0.0	0.8589808553606295	0.0
9	0.0	0.0	0.0	0.9708220012131729	0.0
10	0.0	0.0	0.0	1.1504147087846084	0.0
11	0.0	0.0	0.0	1.291251707265589	0.0
12	0.0	0.0	0.0	1.4270385474609903	0.0
13	0.0	0.0	0.0	1.4778238470743978	0.0
14	0.0	0.0	0.0	1.49592497621035	0.0
15	0.0	0.0	0.0	1.5265663860329646	0.0
16	0.0	0.0	0.0	1.6008434331770431	0.0
17	0.0	0.0	0.0	1.6856180003529437	0.0
18	0.0	0.0	0.0	1.835590678429185	0.0
19	0.0	0.0	0.0	1.8851843676606022	0.0
20	0.0	0.0	0.0	1.9399417018806449	0.0
21	0.0	0.0	0.0	2.015580589461284	0.0
22	0.0	0.0	0.0	2.0899143799568862	0.0
23	0.0	0.0	0.0	2.1867185376557394	0.0
24	0.0	0.0	0.0	2.2622439385333317	0.0
25	0.0	0.0	0.0	2.323016068014851	0.0
26	0.0	0.0	0.0	2.389008585836519	0.0
27	0.0	0.0	0.0	2.445865424062926	0.0
28	0.0	0.0	0.0	2.5084533407931926	0.0
29	0.0	0.0	0.0	2.585056865349729	0.0
30	0.0	0.0	0.0	2.695252454008095	0.0
31	0.0	0.0	0.0	2.8019299548720125	0.0
32	5.674335152336039E-5	0.0	0.0	2.8956132282370803	0.0
33	5.674335152336039E-5	0.0	0.0	2.9822603260132516	0.0
34	1.1348670304672078E-4	0.0	0.0	3.074978962402423	0.0
35	1.1348670304672078E-4	0.0	0.0	3.2122411297374316	0.0
36	1.1348670304672078E-4	0.0	0.0	3.3196562941711525	0.0
37	1.1348670304672078E-4	0.0	0.0	3.424234291028706	0.0
38	1.1348670304672078E-4	0.0	0.0	3.5256914035524742	0.0
39	1.1348670304672078E-4	0.0	0.0	3.6283968698097566	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTC	55	1.8189894E-12	45.000004	6
GCGACGG	110	0.0	45.000004	2
ACGCCAA	20	7.0343114E-4	45.000004	44
ACGCATC	20	7.0343114E-4	45.000004	20
GCGAACC	20	7.0343114E-4	45.000004	27
CCTCGAC	20	7.0343114E-4	45.000004	22
ATCGTAG	20	7.0343114E-4	45.000004	1
CGGATCA	40	6.8193913E-9	45.000004	31
TATAGCG	85	0.0	45.000004	1
CGGTCTA	55	1.8189894E-12	45.000004	31
ACGTAAG	40	6.8193913E-9	45.000004	1
GCGGAAC	40	6.8193913E-9	45.000004	21
ACATCGC	20	7.0343114E-4	45.000004	41
CTCTCGA	20	7.0343114E-4	45.000004	16
CGTTTCA	20	7.0343114E-4	45.000004	44
GTAACGT	55	1.8189894E-12	45.000004	9
ATCGATC	20	7.0343114E-4	45.000004	29
CCTTCGT	35	1.2124292E-7	45.0	7
AGTTTCG	25	3.8916558E-5	45.0	36
CAACGGA	25	3.8916558E-5	45.0	22
>>END_MODULE