##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548936_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1983434
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8812115754797	31.0	31.0	33.0	30.0	33.0
2	31.184181575993957	31.0	31.0	33.0	30.0	34.0
3	31.3201724887241	31.0	31.0	34.0	28.0	34.0
4	35.1939671297356	37.0	35.0	37.0	33.0	37.0
5	35.07752564491685	37.0	35.0	37.0	32.0	37.0
6	35.14914637946108	37.0	35.0	37.0	32.0	37.0
7	35.56390431947824	36.0	35.0	37.0	35.0	37.0
8	35.62737656004687	37.0	35.0	37.0	35.0	37.0
9	37.44245737443242	39.0	37.0	39.0	35.0	39.0
10	36.93919434677433	39.0	37.0	39.0	33.0	39.0
11	36.54672250248811	38.0	35.0	39.0	32.0	39.0
12	35.286498567635725	35.0	35.0	39.0	31.0	39.0
13	34.834671080560284	35.0	34.0	39.0	30.0	39.0
14	35.522315842120285	36.0	34.0	40.0	29.0	41.0
15	35.923755466529265	36.0	34.0	40.0	31.0	41.0
16	36.0967937425697	36.0	34.0	40.0	31.0	41.0
17	35.923684377700496	36.0	34.0	40.0	31.0	41.0
18	36.00425524620431	36.0	35.0	40.0	31.0	41.0
19	35.866302080129714	36.0	35.0	40.0	31.0	41.0
20	35.5877271439332	35.0	34.0	40.0	30.0	41.0
21	35.43759560439117	35.0	34.0	40.0	30.0	41.0
22	35.27803345107526	35.0	34.0	40.0	30.0	41.0
23	35.19786642761998	35.0	34.0	40.0	29.0	41.0
24	35.284534801763	35.0	34.0	39.0	30.0	41.0
25	35.17608652468396	35.0	34.0	39.0	30.0	41.0
26	34.93601249146682	35.0	34.0	39.0	29.0	41.0
27	34.91363513986349	35.0	34.0	39.0	29.0	41.0
28	34.97542343228965	36.0	34.0	39.0	29.0	41.0
29	34.94958138259201	36.0	34.0	39.0	29.0	41.0
30	34.92260695339497	36.0	34.0	39.0	29.0	41.0
31	34.69822943440518	35.0	34.0	39.0	29.0	41.0
32	34.52693863269461	35.0	34.0	39.0	27.0	41.0
33	34.27339906445085	35.0	33.0	40.0	26.0	41.0
34	34.024746475052865	35.0	33.0	39.0	24.0	41.0
35	33.781851072432964	35.0	33.0	39.0	23.0	41.0
36	33.51463522355672	35.0	33.0	39.0	23.0	41.0
37	33.54928976714123	35.0	33.0	39.0	23.0	41.0
38	33.57396414501314	35.0	33.0	39.0	23.0	41.0
39	33.57648653799421	35.0	33.0	39.0	23.0	41.0
40	33.264367253964586	35.0	32.0	39.0	23.0	41.0
41	33.33379230163444	35.0	32.0	39.0	23.0	41.0
42	33.30930799814867	35.0	32.0	39.0	23.0	41.0
43	33.272957406195516	35.0	32.0	39.0	23.0	41.0
44	33.236283637368324	35.0	32.0	39.0	23.0	41.0
45	33.28851930540668	35.0	33.0	39.0	23.0	41.0
46	33.2880862181449	35.0	33.0	39.0	23.0	41.0
47	33.22816136054943	35.0	32.0	39.0	23.0	41.0
48	33.251364552589095	35.0	33.0	39.0	23.0	41.0
49	33.27136068051672	35.0	32.0	39.0	23.0	41.0
50	33.04901095776315	35.0	32.0	39.0	22.0	41.0
51	32.76248062703372	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	2.0
12	4.0
13	7.0
14	14.0
15	52.0
16	178.0
17	534.0
18	1276.0
19	2513.0
20	4549.0
21	7439.0
22	11255.0
23	16474.0
24	22885.0
25	31654.0
26	40478.0
27	46749.0
28	50376.0
29	56305.0
30	66052.0
31	81411.0
32	102462.0
33	135727.0
34	242289.0
35	346707.0
36	116352.0
37	139626.0
38	198548.0
39	261435.0
40	76.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.85604663427167	24.763667457550895	27.48727711635477	14.893008791822668
2	30.373181058709285	25.970362512692635	30.98182243523102	12.67463399336706
3	28.22387838466014	25.7118714310635	34.32380406910439	11.74044611517197
4	26.58712112427235	27.768960298149576	33.02449186612713	12.619426711450949
5	22.538435864263697	32.509072648749594	32.32177123110726	12.63072025587945
6	19.56999829588481	41.15539009616655	29.787076353435506	9.487535254513132
7	84.6918526152118	5.243532177022275	8.597765289896211	1.466849917869715
8	86.87286796535705	3.450429910952419	8.360701692115795	1.3160004315747336
9	82.40884244194663	5.486545052671276	10.00512242908007	2.0994900763020095
10	55.79000864157819	21.11721388258949	15.133349534191709	7.95942794164061
11	52.82772202150412	17.911763134039248	20.559645544041295	8.70086930041534
12	48.260088311484026	21.571779045836664	21.82174955153537	8.346383091143945
13	17.840775140488667	50.62679171578181	21.909022432810975	9.623410710918538
14	10.5872945608475	51.23316429989604	27.13884102017007	11.040700119086393
15	9.899598373326262	23.795447693243133	56.023895929988086	10.281058003442514
16	10.336164450140513	17.34506920825195	54.9482362407824	17.370530100825135
17	10.730228482520719	18.59184626259306	32.42058974485665	38.25733551002958
18	15.115854623849344	23.3959385590849	44.56992266947123	16.918284147594527
19	31.43568175195141	19.787903202224022	29.72823900366738	19.048176042157188
20	31.30903271800322	20.52213484290377	31.778521493530913	16.390310945562092
21	16.766627979554652	33.736237253167985	33.40811945343278	16.089015313844573
22	19.577258431588852	24.881896750786765	23.22774541527472	32.31309940234966
23	13.430545205940808	33.62204136865658	27.17625088608948	25.77116253931313
24	11.277309958385304	21.133599605532627	54.3363681372811	13.252722298800968
25	10.571463431603975	25.356830628092492	48.09723943423376	15.974466506069776
26	10.087202296622928	40.31603774060544	30.75338024859915	18.84337971417249
27	10.312316921057116	48.76355855551534	27.659554086498467	13.264570436929082
28	10.245866512321559	34.8307027105515	42.138130131882384	12.78530064524456
29	10.167215042194497	26.074424457783824	40.99909550809354	22.75926499192814
30	13.084831660645124	43.33070825648849	30.541424620128527	13.043035462737857
31	30.060087706472714	30.932917354446882	28.37795459793469	10.629040341145709
32	33.11216808827518	23.688058185954258	31.364492087964614	11.835281637805947
33	32.99323294851253	29.588330138537504	22.3141279215744	15.104308991375564
34	18.47129775934062	28.1788554597733	25.466236839743594	27.883609941142485
35	19.276214887916613	25.40946661194676	34.94862949813304	20.365689002003595
36	36.16036631418035	27.35241001213048	23.970094291012455	12.517129382676712
37	15.971138943872093	43.70284062892942	28.69946769088359	11.626552736314895
38	15.980163695893083	41.88578999855805	24.41578595506581	17.71826035048305
39	19.547713712682146	38.91982289302291	27.43811994752535	14.094343446769592
40	27.05242523824841	25.078979184585926	26.63290031329502	21.235695263870642
41	13.557395910325223	25.300211653122815	30.125529763027153	31.016862673524802
42	15.87000122010614	22.736324979807748	28.375635387918123	33.01803841216799
43	17.300500041846615	22.378057449857167	30.365013406042245	29.956429102253967
44	12.494592711428766	30.51182948361276	35.31859391338457	21.674983891573905
45	12.276939893134836	50.66339489995634	22.94565889260747	14.114006314301358
46	18.026916953122715	42.57772126523998	24.650227837175322	14.745133944461978
47	17.307810595159708	31.825309034734705	25.903811268738963	24.96306910136662
48	18.10723220434862	28.963857632772257	38.428200787119714	14.500709375759415
49	22.735518298062853	27.79845459944722	36.90705110429689	12.558975998193034
50	19.241527572886216	42.465037909000245	24.879476705552086	13.413957812561447
51	16.608266269510356	38.84591067814709	24.716274905038432	19.82954814730412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2825.0
1	4517.0
2	6209.0
3	14753.0
4	23297.0
5	18184.5
6	13072.0
7	12954.5
8	12837.0
9	13616.0
10	14395.0
11	14364.5
12	14334.0
13	13837.5
14	13341.0
15	12583.0
16	11825.0
17	11460.5
18	11096.0
19	10697.5
20	10299.0
21	11597.5
22	12896.0
23	14046.0
24	15196.0
25	15613.0
26	21401.5
27	26773.0
28	32645.0
29	38517.0
30	49528.5
31	60540.0
32	65175.0
33	69810.0
34	72069.0
35	74328.0
36	81687.0
37	89046.0
38	104862.5
39	120679.0
40	158834.5
41	196990.0
42	248097.5
43	299205.0
44	281952.5
45	264700.0
46	226333.0
47	187966.0
48	158505.0
49	129044.0
50	108950.5
51	88857.0
52	68418.5
53	47980.0
54	36889.0
55	25798.0
56	22243.5
57	18689.0
58	17338.0
59	15987.0
60	14659.0
61	13331.0
62	11910.5
63	10490.0
64	9047.5
65	7605.0
66	6415.0
67	5225.0
68	4727.5
69	4230.0
70	4030.5
71	3831.0
72	3339.5
73	2848.0
74	2336.0
75	1288.5
76	753.0
77	555.0
78	357.0
79	269.0
80	181.0
81	139.5
82	98.0
83	76.0
84	54.0
85	42.5
86	31.0
87	18.0
88	5.0
89	5.5
90	6.0
91	3.0
92	0.0
93	1.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1983434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.47465043433775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55135843132368	14.07606830598426
2	7.442719578115085	2.6011784581672575
3	2.6710952786209123	1.4002936881213135
4	1.4541880049987612	1.016457082126414
5	1.005094544438811	0.8781837908764087
6	0.7642903933507857	0.8013424472476487
7	0.55613219246554	0.6802750961032008
8	0.4472248467902894	0.6252078290568452
9	0.3492719425252598	0.5493064591935919
>10	3.1805670521745295	11.43151865520913
>50	0.5039934313413996	6.240486088495138
>100	0.8961162513546493	38.57606197749218
>500	0.1649062571216086	17.33656695212764
>1k	0.01275197770360418	3.190003678394405
>5k	0.0	0.0
>10k+	2.8981767508191316E-4	0.5970494914045792
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11789	0.5943731931589356	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3833	0.19325069551091695	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3075	0.1550341478466135	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2926	0.14752192409729792	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2462	0.1241281534954024	No Hit
AAGTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2025	0.10209565833801378	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1989	0.10028062441200464	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02495671648262559	0.0
2	0.0	0.0	0.0	0.09544053394264694	0.0
3	0.0	0.0	0.0	0.1343629281337317	0.0
4	0.0	0.0	0.0	0.18841060504155924	0.0
5	0.0	0.0	0.0	0.3091607787302224	0.0
6	0.0	0.0	0.0	0.4330872617894016	0.0
7	0.0	0.0	0.0	0.5156208878137614	0.0
8	0.0	0.0	0.0	0.7214255679795748	0.0
9	0.0	0.0	0.0	0.8049675461850507	0.0
10	0.0	0.0	0.0	0.9587412538052691	0.0
11	0.0	0.0	0.0	1.1074732005199064	0.0
12	0.0	0.0	0.0	1.237903555147285	0.0
13	0.0	0.0	0.0	1.296337564042968	0.0
14	0.0	0.0	0.0	1.3195296642086403	0.0
15	0.0	0.0	0.0	1.3559815955559902	0.0
16	0.0	0.0	0.0	1.4359439235185039	0.0
17	0.0	0.0	0.0	1.5276535543910208	0.0
18	0.0	0.0	0.0	1.6882840568428292	0.0
19	0.0	0.0	0.0	1.7474239122652935	0.0
20	0.0	0.0	0.0	1.813370144910292	0.0
21	0.0	0.0	0.0	1.9067435568816506	0.0
22	0.0	0.0	0.0	1.9983523525360563	0.0
23	0.0	0.0	0.0	2.1158757992451473	0.0
24	0.0	0.0	0.0	2.2030982629116975	0.0
25	0.0	0.0	0.0	2.270909947091761	0.0
26	0.0	0.0	0.0	2.3434104689140147	0.0
27	0.0	0.0	0.0	2.412028834838971	0.0
28	0.0	0.0	0.0	2.4867477314596806	0.0
29	0.0	0.0	0.0	2.569634280747431	0.0
30	0.0	0.0	0.0	2.6831747363411136	0.0
31	0.0	0.0	0.0	2.788144198395308	0.0
32	0.0	0.0	0.0	2.886609788881304	0.0
33	0.0	0.0	0.0	2.9835124334865695	0.0
34	0.0	0.0	0.0	3.087423125750592	0.0
35	0.0	0.0	0.0	3.2319199933045417	0.0
36	0.0	0.0	0.0	3.346166295425005	0.0
37	0.0	0.0	0.0	3.4590513221009624	0.0
38	0.0	0.0	0.0	3.571785095949752	0.0
39	0.0	0.0	0.0	3.69066981810335	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGCT	20	7.0345256E-4	45.0	19
AAGTACG	75	0.0	45.0	1
GATCGCT	40	6.8193913E-9	45.0	10
ACGTTAT	20	7.0345256E-4	45.0	33
ACGTTAG	45	3.8562575E-10	45.0	1
ATTACCG	35	1.2125201E-7	45.0	13
TCGGGCA	20	7.0345256E-4	45.0	1
TACGATA	25	3.8918333E-5	45.0	34
CTACGAT	20	7.0345256E-4	45.0	33
GCGATAA	160	0.0	45.0	9
GCTAGAT	25	3.8918333E-5	45.0	23
TAGGCGT	20	7.0345256E-4	45.0	15
CGGTATA	25	3.8918333E-5	45.0	41
AGTAGCG	155	0.0	45.0	1
TACAGCC	20	7.0345256E-4	45.0	43
AAAATCG	55	1.8189894E-12	45.0	27
CCGTTTA	20	7.0345256E-4	45.0	18
GTACGTG	55	1.8189894E-12	45.0	1
TACGTAG	25	3.8918333E-5	45.0	1
GTTGCGA	20	7.0345256E-4	45.0	16
>>END_MODULE