FastQCFastQC Report
Sat 18 Jun 2016
SRR3548935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066473
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102020.4936914249544998No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG27310.1321575457313016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26410.12780229889284786No Hit
GGATTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA24260.11739809811209727No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA23270.11260732658979818No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT22990.11125236090672368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC302.1663454E-645.00000427
TACCCGT600.045.00000438
ACGCATG207.0345943E-445.0000041
GCACGAG850.045.0000041
GTTAGCG207.0345943E-445.0000041
CTAAGCG406.8193913E-945.0000041
TCTCGTC207.0345943E-445.00000433
GAACGAT207.0345943E-445.0000049
CGTAATT406.8193913E-945.00000430
ATAACCG207.0345943E-445.00000437
TCATACG351.2125565E-745.00000410
CGCGTCA207.0345943E-445.00000439
CCAAACG302.1663454E-645.00000427
CGATAGC207.0345943E-445.00000410
TATTGCG453.8562575E-1045.01
TGCGCAC502.1827873E-1145.012
CGGATGG453.8562575E-1045.017
GTATACG453.8562575E-1045.01
CACACGG502.1827873E-1145.02
ATTGCGA253.8918897E-545.010