##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2066473
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.863353162610885	31.0	31.0	33.0	30.0	33.0
2	31.18003816164063	31.0	31.0	33.0	30.0	34.0
3	31.32341579106042	31.0	31.0	34.0	28.0	34.0
4	35.2099867745671	37.0	35.0	37.0	33.0	37.0
5	35.10568296803297	37.0	35.0	37.0	32.0	37.0
6	35.1590274830593	37.0	35.0	37.0	32.0	37.0
7	35.49663170048677	36.0	35.0	37.0	35.0	37.0
8	35.54733548418005	37.0	35.0	37.0	35.0	37.0
9	37.368215311789704	39.0	37.0	39.0	35.0	39.0
10	36.89360180365289	39.0	37.0	39.0	32.0	39.0
11	36.52479272654421	38.0	35.0	39.0	32.0	39.0
12	35.34504201119492	35.0	35.0	39.0	31.0	39.0
13	34.935129082257546	35.0	34.0	39.0	30.0	39.0
14	35.620566056270754	36.0	34.0	40.0	30.0	41.0
15	35.991977635323565	37.0	34.0	40.0	31.0	41.0
16	36.16605201229341	36.0	35.0	40.0	32.0	41.0
17	36.00380213048997	36.0	34.0	40.0	31.0	41.0
18	36.07928388127984	36.0	35.0	40.0	31.0	41.0
19	35.95001628378401	36.0	35.0	40.0	31.0	41.0
20	35.67393186361496	36.0	34.0	40.0	31.0	41.0
21	35.547018518993475	35.0	34.0	40.0	30.0	41.0
22	35.377439240677234	35.0	34.0	40.0	30.0	41.0
23	35.28010770041515	35.0	34.0	40.0	30.0	41.0
24	35.36659999912895	35.0	34.0	40.0	30.0	41.0
25	35.25970869205646	35.0	34.0	39.0	30.0	41.0
26	35.01682383462063	35.0	34.0	39.0	29.0	41.0
27	34.9993046122548	35.0	34.0	39.0	29.0	41.0
28	35.05490756472501	36.0	34.0	39.0	29.0	41.0
29	35.01720322501189	36.0	34.0	39.0	29.0	41.0
30	34.99098754254229	36.0	34.0	39.0	29.0	41.0
31	34.75955601645896	35.0	34.0	39.0	29.0	41.0
32	34.61992631890182	35.0	34.0	40.0	27.0	41.0
33	34.34893124662166	35.0	33.0	40.0	26.0	41.0
34	34.136029844087005	35.0	33.0	40.0	24.0	41.0
35	33.91275327575052	35.0	33.0	40.0	23.0	41.0
36	33.65946179795235	35.0	33.0	40.0	23.0	41.0
37	33.669748890984785	35.0	33.0	39.0	23.0	41.0
38	33.69385034307247	35.0	33.0	40.0	23.0	41.0
39	33.70131184873937	35.0	33.0	40.0	23.0	41.0
40	33.40952434413612	35.0	33.0	39.0	23.0	41.0
41	33.45140439773469	35.0	33.0	39.0	23.0	41.0
42	33.40498085385098	35.0	33.0	39.0	23.0	41.0
43	33.37133124894446	35.0	33.0	39.0	23.0	41.0
44	33.33011028936744	35.0	33.0	39.0	23.0	41.0
45	33.40920544328428	35.0	33.0	39.0	23.0	41.0
46	33.406742793155296	35.0	33.0	39.0	23.0	41.0
47	33.34283486888045	35.0	33.0	39.0	23.0	41.0
48	33.37070554514867	35.0	33.0	39.0	23.0	41.0
49	33.38341270367433	35.0	33.0	39.0	23.0	41.0
50	33.20329179234377	35.0	32.0	39.0	23.0	41.0
51	32.92904141501002	35.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	3.0
13	6.0
14	12.0
15	66.0
16	202.0
17	570.0
18	1302.0
19	2764.0
20	4841.0
21	7798.0
22	11808.0
23	17131.0
24	23869.0
25	32438.0
26	41611.0
27	46900.0
28	50767.0
29	57603.0
30	67336.0
31	82725.0
32	104431.0
33	138315.0
34	246901.0
35	356679.0
36	123834.0
37	148977.0
38	211144.0
39	286339.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.687288921752184	23.933387951354796	28.696334285519338	14.682988841373684
2	31.581540141100316	26.15122481639005	30.60185156060592	11.665383481903707
3	28.236758960799392	26.15122481639005	33.0227397115762	12.58927651123436
4	25.873456851359784	28.121683661001136	32.24905430654066	13.75580518109842
5	22.639056982597886	32.93210218570482	31.56876475037419	12.860076081323104
6	21.126673322129058	40.4998758754651	29.236675243276828	9.136775559129008
7	84.58237780024224	5.3336288448965945	8.637519096547596	1.4464742583135615
8	86.00920505615123	4.373926008227545	8.036930557524826	1.5799383780963991
9	81.26667999049589	6.192870654491978	10.184454381934824	2.3559949730773155
10	55.045626049795956	22.266489811383938	14.963999045717028	7.723885093103079
11	51.66905156757432	18.188381846750477	21.116559471137535	9.02600711453767
12	48.19883927832592	20.7603970630151	22.32605990980768	8.714703748851303
13	18.194140450903546	49.1619779208342	21.270977167376493	11.37290446088577
14	10.698905816819286	50.143021467011664	26.54310024858781	12.614972467581238
15	9.490953910358375	25.153050632647993	54.452828563450865	10.903166893542766
16	10.94236411508885	17.86740015475644	53.22973007631844	17.96050565383627
17	10.298561849102311	19.113678233395742	32.18174154707078	38.40601837043117
18	15.428558708485424	22.802233564145286	44.01305993351958	17.756147793849713
19	31.42450929675829	21.017453409746945	28.3347520146646	19.22328527883016
20	32.079102896577886	20.887618662329483	30.730089384182612	16.30318905691001
21	16.61637969622637	34.32273250122309	31.72090803993084	17.339979762619688
22	19.80422681544835	26.069588133984812	23.132845190815463	30.993339859751373
23	13.728947825594624	34.496119717025095	26.20943994913072	25.565492508249566
24	11.006192677087965	22.125718555238805	53.74887549946212	13.119213268211102
25	10.840983647015955	27.03209768528309	47.02853606120186	15.098382606499092
26	10.802560691574485	40.09178924670199	30.212734451405847	18.892915610317676
27	10.287141424059254	48.7806518643118	26.943492607936324	13.98871410369262
28	10.028342978592027	34.757870052016166	41.78360907691511	13.4301778924767
29	9.936350487037576	26.25826710535294	40.86344220321291	22.941940204396573
30	13.383044443358322	42.87735673294546	30.0964009691876	13.643197854508623
31	29.57135176699623	32.48849609939254	26.18858315593768	11.751568977673553
32	33.90777426078154	23.419275257891105	29.604451642968478	13.068498838358886
33	32.53209695940861	30.130371894527535	21.30427060987489	16.033260536188955
34	18.05370793617918	27.849190383808548	25.903798404334346	28.193303275677927
35	18.723012591986443	25.61707798746947	34.077774062375845	21.58213535816824
36	34.69970331090704	28.431148144689043	23.285811138108265	13.583337406295653
37	15.485322092279938	43.32139834394159	29.653762715506083	11.539516848272395
38	14.893686005091766	42.58487771192752	25.153099024279534	17.368337258701178
39	18.43934084790849	40.274419264127815	27.257263946831145	14.028975941132549
40	26.962268560973214	25.702779566923933	25.758139593403833	21.57681227869902
41	13.976664587439567	24.828294393393964	29.446404574364145	31.748636444802326
42	14.878732990946409	22.845302116214437	27.147947251185954	35.128017641653194
43	15.48227341949302	23.225708731737605	30.383072994420928	30.908944854348448
44	11.99284965252389	29.93283725458789	35.363442929087384	22.710870163800834
45	11.065424034090936	51.08602919079998	22.874724228189773	14.973822546919315
46	17.436956592222593	43.288395251232416	24.067335987453017	15.207312169091974
47	15.893263546148436	32.17666042575925	26.63310868324919	25.296967344843125
48	16.376695945216802	28.242662739847074	39.214448966911256	16.16619234802487
49	20.38395856127808	28.964472315873476	37.597926515371846	13.053642607476604
50	18.16505708034898	42.175242550955176	26.10191374385245	13.557786624843393
51	14.763318949727386	39.21788477275048	25.30495196404695	20.71384431347518
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3449.0
1	4487.0
2	5525.0
3	13395.5
4	21266.0
5	16988.0
6	12710.0
7	13124.5
8	13539.0
9	14193.5
10	14848.0
11	14487.0
12	14126.0
13	13796.5
14	13467.0
15	13068.0
16	12669.0
17	11742.0
18	10815.0
19	10890.0
20	10965.0
21	10459.0
22	9953.0
23	13042.5
24	16132.0
25	17623.0
26	21764.0
27	24414.0
28	33568.0
29	42722.0
30	49475.0
31	56228.0
32	69425.5
33	82623.0
34	82706.5
35	82790.0
36	92479.0
37	102168.0
38	114012.5
39	125857.0
40	170531.5
41	215206.0
42	259178.0
43	303150.0
44	290214.0
45	277278.0
46	234573.0
47	191868.0
48	157101.5
49	122335.0
50	103337.5
51	84340.0
52	66776.0
53	49212.0
54	38150.0
55	27088.0
56	23858.5
57	20629.0
58	19164.5
59	17700.0
60	16885.5
61	16071.0
62	14467.5
63	12864.0
64	11051.0
65	9238.0
66	8039.0
67	6840.0
68	6074.0
69	5308.0
70	4805.0
71	4302.0
72	3884.0
73	3466.0
74	2783.5
75	1544.0
76	987.0
77	757.0
78	527.0
79	413.0
80	299.0
81	236.0
82	173.0
83	116.5
84	60.0
85	48.0
86	36.0
87	23.5
88	11.0
89	6.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2066473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.291130175433523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.75361488821639	13.963212671689758
2	7.18102875876981	2.4833620612284113
3	2.65453704170412	1.3769983663084844
4	1.457166171230685	1.0078419981595133
5	1.0123673454225004	0.8752487777529263
6	0.7301936315020852	0.7575523881345054
7	0.5651154915176291	0.6840039869589796
8	0.4749116872497242	0.6569407844855802
9	0.3958030794177114	0.615949431304419
>10	3.1936787677034215	10.991081503333675
>50	0.4611198570607951	5.691852731931615
>100	0.9593527508311699	42.07694493837087
>500	0.14986372104076895	15.62031873958312
>1k	0.010965638124934313	2.702695817025632
>5k	0.0	0.0
>10k+	2.8117020833164904E-4	0.4959958037325376
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10202	0.4936914249544998	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2731	0.1321575457313016	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2641	0.12780229889284786	No Hit
GGATTAGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	2426	0.11739809811209727	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	2327	0.11260732658979818	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2299	0.11125236090672368	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02245371703380591	0.0
2	0.0	0.0	0.0	0.08013654182754867	0.0
3	0.0	0.0	0.0	0.11280089311595168	0.0
4	0.0	0.0	0.0	0.17145155054046193	0.0
5	0.0	0.0	0.0	0.2841072687617985	0.0
6	0.0	0.0	0.0	0.4259915324323134	0.0
7	0.0	0.0	0.0	0.5244685026129061	0.0
8	0.0	0.0	0.0	0.7793956175570647	0.0
9	0.0	0.0	0.0	0.8914222445684022	0.0
10	0.0	0.0	0.0	1.0682452662096238	0.0
11	0.0	0.0	0.0	1.2093068721439864	0.0
12	0.0	0.0	0.0	1.349594211973735	0.0
13	0.0	0.0	0.0	1.4012764744567192	0.0
14	0.0	0.0	0.0	1.4241173245428322	0.0
15	0.0	0.0	0.0	1.4511682465727838	0.0
16	0.0	0.0	0.0	1.5123352688372895	0.0
17	0.0	0.0	0.0	1.587439080984847	0.0
18	0.0	0.0	0.0	1.721435508714607	0.0
19	0.0	0.0	0.0	1.7687625243591376	0.0
20	0.0	0.0	0.0	1.8282358395198002	0.0
21	0.0	0.0	0.0	1.8987424466712122	0.0
22	0.0	0.0	0.0	1.970362061348007	0.0
23	0.0	0.0	0.0	2.063951476743224	0.0
24	0.0	0.0	0.0	2.1324740270015625	0.0
25	0.0	0.0	0.0	2.1856564300622363	0.0
26	0.0	0.0	0.0	2.2444038707498235	0.0
27	0.0	0.0	0.0	2.304167535699716	0.0
28	0.0	0.0	0.0	2.365963649174221	0.0
29	0.0	0.0	0.0	2.4422772521102383	0.0
30	0.0	0.0	0.0	2.5488356247577393	0.0
31	0.0	0.0	0.0	2.642231473626803	0.0
32	0.0	0.0	0.0	2.7294815852904923	0.0
33	0.0	0.0	0.0	2.817457571427258	0.0
34	0.0	0.0	0.0	2.908869363403248	0.0
35	0.0	0.0	0.0	3.034639213771484	0.0
36	0.0	0.0	0.0	3.1303094693228513	0.0
37	0.0	0.0	0.0	3.233819169183435	0.0
38	0.0	0.0	0.0	3.3272634096840363	0.0
39	0.0	0.0	0.0	3.4235627564454023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATC	30	2.1663454E-6	45.000004	27
TACCCGT	60	0.0	45.000004	38
ACGCATG	20	7.0345943E-4	45.000004	1
GCACGAG	85	0.0	45.000004	1
GTTAGCG	20	7.0345943E-4	45.000004	1
CTAAGCG	40	6.8193913E-9	45.000004	1
TCTCGTC	20	7.0345943E-4	45.000004	33
GAACGAT	20	7.0345943E-4	45.000004	9
CGTAATT	40	6.8193913E-9	45.000004	30
ATAACCG	20	7.0345943E-4	45.000004	37
TCATACG	35	1.2125565E-7	45.000004	10
CGCGTCA	20	7.0345943E-4	45.000004	39
CCAAACG	30	2.1663454E-6	45.000004	27
CGATAGC	20	7.0345943E-4	45.000004	10
TATTGCG	45	3.8562575E-10	45.0	1
TGCGCAC	50	2.1827873E-11	45.0	12
CGGATGG	45	3.8562575E-10	45.0	17
GTATACG	45	3.8562575E-10	45.0	1
CACACGG	50	2.1827873E-11	45.0	2
ATTGCGA	25	3.8918897E-5	45.0	10
>>END_MODULE