FastQCFastQC Report
Sat 18 Jun 2016
SRR3548933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319275
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119020.35857227858493196No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG44880.13521024922611113No Hit
TATTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA42980.12948610765905205No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39200.11809807864669242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCAG302.1667456E-645.0000041
CAACGAC302.1667456E-645.00000413
ACGTTGA600.045.00000441
CGTTGAA1250.045.0000042
GCTATCG600.045.0000041
TACGGTG302.1667456E-645.00000435
CCCCGAT302.1667456E-645.00000440
ACGGACG1250.045.0000041
TTGCGTC302.1667456E-645.00000432
TCATCCG302.1667456E-645.0000041
AGTCGTG302.1667456E-645.0000041
CTTAACG1650.045.01
GTCATCG453.8562575E-1045.01
CGGAATA253.8924034E-545.029
AGCGTCG207.0352154E-445.01
TCGTTAG3450.045.01
CGTATAT253.8924034E-545.023
GGTCGTA502.1827873E-1145.08
ACGTTAG1100.045.01
CCTAGCG453.8562575E-1045.01
Produced by FastQC (version 0.11.5)