##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3319275
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89140007983671	31.0	31.0	33.0	30.0	33.0
2	31.208804332271356	31.0	31.0	33.0	30.0	34.0
3	31.375760068087157	31.0	31.0	34.0	30.0	34.0
4	35.19029697750262	37.0	35.0	37.0	33.0	37.0
5	35.055802547243	37.0	35.0	37.0	32.0	37.0
6	35.12211522094433	37.0	35.0	37.0	32.0	37.0
7	35.560847775493144	36.0	35.0	37.0	35.0	37.0
8	35.64470193039143	37.0	35.0	37.0	35.0	37.0
9	37.52393820939814	39.0	37.0	39.0	35.0	39.0
10	37.06422637473545	39.0	37.0	39.0	33.0	39.0
11	36.51812278283661	39.0	35.0	39.0	32.0	39.0
12	34.63756844491643	35.0	33.0	39.0	30.0	39.0
13	33.95743980236648	35.0	33.0	38.0	25.0	39.0
14	34.47186629610382	35.0	33.0	39.0	25.0	41.0
15	35.0981289588841	35.0	33.0	39.0	30.0	41.0
16	35.43064313743212	35.0	34.0	39.0	31.0	41.0
17	35.31515828004609	35.0	34.0	39.0	31.0	41.0
18	35.464302596199474	36.0	34.0	39.0	31.0	41.0
19	35.30190659104775	36.0	34.0	38.0	30.0	41.0
20	34.96143736207455	35.0	34.0	38.0	30.0	41.0
21	34.77023175241581	35.0	34.0	38.0	29.0	41.0
22	34.60012382222021	35.0	33.0	38.0	29.0	41.0
23	34.597711247185	35.0	34.0	38.0	29.0	41.0
24	34.74317072252224	35.0	34.0	38.0	30.0	41.0
25	34.67554360515474	35.0	34.0	38.0	30.0	41.0
26	34.42082864480948	35.0	34.0	38.0	29.0	40.0
27	34.39060849131211	35.0	34.0	38.0	29.0	40.0
28	34.55142162068524	35.0	34.0	38.0	29.0	40.0
29	34.58761446400193	35.0	34.0	38.0	29.0	40.0
30	34.55986442822604	35.0	34.0	38.0	29.0	40.0
31	34.20409155613801	35.0	34.0	38.0	29.0	40.0
32	34.029658283811976	35.0	34.0	38.0	27.0	41.0
33	33.797063816646705	35.0	33.0	38.0	27.0	41.0
34	33.63831378840259	35.0	33.0	38.0	25.0	41.0
35	33.40351432165156	35.0	33.0	38.0	23.0	41.0
36	33.08437866702819	35.0	33.0	38.0	23.0	40.0
37	33.117622974896626	35.0	32.0	38.0	23.0	40.0
38	33.24232882180597	35.0	32.0	38.0	23.0	40.0
39	33.28727267249625	35.0	33.0	38.0	23.0	40.0
40	32.87420807254596	35.0	32.0	38.0	23.0	40.0
41	33.06835769859382	35.0	32.0	38.0	23.0	40.0
42	33.048312959908415	35.0	32.0	38.0	23.0	40.0
43	33.010393233462125	35.0	32.0	38.0	23.0	40.0
44	32.98038427066152	35.0	32.0	37.0	23.0	40.0
45	33.09648281627765	35.0	33.0	37.0	23.0	40.0
46	33.136971175934505	35.0	33.0	37.0	23.0	40.0
47	33.08014460989222	35.0	33.0	37.0	23.0	40.0
48	33.1347233959223	35.0	33.0	37.0	23.0	40.0
49	33.24432564340104	35.0	33.0	37.0	24.0	40.0
50	32.98488314466262	35.0	32.0	37.0	24.0	40.0
51	32.684971868856906	35.0	32.0	37.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	4.0
11	2.0
12	6.0
13	10.0
14	22.0
15	82.0
16	294.0
17	847.0
18	2182.0
19	4524.0
20	8085.0
21	13085.0
22	19064.0
23	27372.0
24	38510.0
25	52706.0
26	67206.0
27	77540.0
28	85929.0
29	97650.0
30	116310.0
31	144575.0
32	185933.0
33	251195.0
34	487842.0
35	737727.0
36	145364.0
37	168397.0
38	240765.0
39	345860.0
40	183.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.222706765784697	25.197550669950513	32.05980221584533	13.51994034841946
2	28.56590671155599	27.93423262610058	31.740003464612	11.759857197731433
3	28.75751481874807	26.055298220243877	32.96963945439893	12.217547506609124
4	24.537677655512123	27.924079806584267	33.47164666983001	14.0665958680736
5	22.546791091428098	32.83063319550203	31.940589435946105	12.68198627712377
6	20.399093175467534	41.05893606284505	29.73031761453932	8.811653147148098
7	87.0124650714388	4.894502564566058	6.892017082043518	1.201015281951631
8	90.61105211228355	2.737525513854682	5.824434552726123	0.8269878211356395
9	87.71481724171693	4.099841079753862	6.753131331390137	1.4322103471390588
10	71.0703994095096	13.96094026557004	10.150017699648267	4.818642625272085
11	68.49827145988205	12.142983030932958	14.41100842804528	4.947737081139707
12	60.26677512408584	19.200006025412176	14.885961542806788	5.647257307695205
13	16.644387705146453	61.08174224793065	14.919523088626283	7.354346958296616
14	7.502300954274653	60.890465538408236	23.48576119785194	8.121472309465169
15	6.334455566351085	21.087165118888915	65.86022550105068	6.7181538137093195
16	6.869451913444954	13.39644952587538	64.61007298280498	15.124025577874686
17	7.287464883144662	13.949732998922956	29.984559881299376	48.778242236633
18	13.931024094116939	20.73591371609764	49.398859690745724	15.934202499039701
19	36.857084754954016	15.633203033795033	27.80546956790263	19.704242643348323
20	36.552319407099446	18.221539342175625	29.831183014363077	15.394958236361855
21	15.314217713205444	35.99364319015448	32.99892295757357	15.693216139066513
22	19.811314217713203	23.09227165570795	18.96543672940627	38.13097739717258
23	10.98420588833405	36.425755624345676	23.678935912209745	28.91110257511053
24	8.855518147788297	18.392480285604538	61.45227496968465	11.299726596922522
25	7.364108126021495	21.87131225945425	55.06847127761333	15.696108336910921
26	6.799014845109248	46.26133718959712	28.93571638384888	18.003931581444743
27	7.6979460876245565	57.65084242793984	25.18140256531923	9.469808919116375
28	6.505276001536481	36.91604341309473	46.25464898208193	10.324031603286862
29	7.118180928064111	24.942886624338144	44.37984198356569	23.559090464032057
30	12.568256622304569	48.4143977223942	27.9847557071951	11.032589948106137
31	36.681564498271456	30.615661552598084	24.0764022263898	8.626371722740659
32	40.86555045906109	20.13487885155644	30.551219769377347	8.44835092000512
33	40.793576910620544	26.82088709130759	18.41628066369915	13.969255334372718
34	18.975920946592254	26.543115590000827	22.36961384639718	32.111349617009736
35	17.786143058348586	22.26281341558021	37.14931724548282	22.80172628058838
36	44.86594211085252	23.04638814198884	21.077373824103155	11.010295923055487
37	14.357623276167237	47.89244639266105	28.338387147795828	9.411543183375889
38	15.296442747286681	46.30050236874016	20.179225885170705	18.22382899880245
39	19.31479012736215	42.44637759751753	25.977088370201322	12.261743904918996
40	30.489399040453115	21.784968102974293	23.301052187601208	24.424580668971387
41	11.339283427856987	22.076326908737602	27.93224424008255	38.652145423322864
42	15.16563104894894	17.46504884349745	26.796002139021326	40.57331796853229
43	16.291419059885065	17.37852392465222	30.42278810884907	35.907268906613645
44	9.448991120048806	30.85628638784072	37.36071883167258	22.334003660437894
45	9.513613665634816	58.94335359378178	19.231579185213636	12.311453555369772
46	18.75460755737322	45.945304320973705	21.495236158498468	13.804851963154604
47	16.633361200864645	30.998636750495212	22.902591680412137	29.465410368228
48	17.49502526907231	27.588976508424278	41.87248721482854	13.04351100767487
49	24.205255665770387	24.68168500651498	40.37935995059162	10.733699377123017
50	19.22124560333205	48.71774709838745	21.087406135375947	10.97360116290455
51	15.27704092008044	43.352629715826495	21.301157632314286	20.069171731778777
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4854.0
1	5817.5
2	6781.0
3	14513.0
4	22245.0
5	18066.0
6	13887.0
7	15347.0
8	16807.0
9	17909.0
10	19011.0
11	19988.0
12	20965.0
13	20011.5
14	19058.0
15	17541.5
16	16025.0
17	15392.0
18	14759.0
19	15658.5
20	16558.0
21	13942.5
22	11327.0
23	12343.5
24	13360.0
25	14938.5
26	23195.0
27	29873.0
28	40607.0
29	51341.0
30	58197.0
31	65053.0
32	76346.0
33	87639.0
34	96477.5
35	105316.0
36	112040.5
37	118765.0
38	148693.0
39	178621.0
40	287159.5
41	395698.0
42	505777.0
43	615856.0
44	587817.5
45	559779.0
46	458509.5
47	357240.0
48	289027.5
49	220815.0
50	180490.5
51	140166.0
52	105143.0
53	70120.0
54	51082.0
55	32044.0
56	25617.0
57	19190.0
58	18041.0
59	16892.0
60	15872.0
61	14852.0
62	12408.0
63	9964.0
64	8192.0
65	6420.0
66	5050.0
67	3680.0
68	3104.5
69	2529.0
70	2242.0
71	1955.0
72	1723.5
73	1492.0
74	1208.0
75	670.0
76	416.0
77	321.5
78	227.0
79	184.0
80	141.0
81	103.5
82	66.0
83	46.0
84	26.0
85	18.5
86	11.0
87	8.5
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3319275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.32770442133441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40373698374253	10.715972408900344
2	8.171658037064166	2.178188859004259
3	2.815814162364779	1.1258501658421536
4	1.3313588598751127	0.7097582945256112
5	0.8233597426112254	0.5486747640974194
6	0.6074842624793779	0.4857822414562486
7	0.453654366522357	0.42323199145203755
8	0.35240016349562486	0.37573481736796865
9	0.3131360036271667	0.37560456900186995
>10	2.9216939396861026	8.210729123044489
>50	0.42395172136052645	3.967171915597936
>100	1.0414713001746008	37.78429638851779
>500	0.29698027892419937	24.685879682475008
>1k	0.04307347556915869	8.053514900009137
>5k	0.0	0.0
>10k+	2.2670250299557205E-4	0.3596098787077279
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11902	0.35857227858493196	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4488	0.13521024922611113	No Hit
TATTTTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	4298	0.12948610765905205	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3920	0.11809807864669242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02072741788492969	0.0
2	0.0	0.0	0.0	0.07649260757243675	0.0
3	0.0	0.0	0.0	0.10601712723410986	0.0
4	0.0	0.0	0.0	0.15503385528466307	0.0
5	0.0	0.0	0.0	0.26746804648605493	0.0
6	0.0	0.0	0.0	0.3634528624473718	0.0
7	0.0	0.0	0.0	0.4323233236173562	0.0
8	0.0	0.0	0.0	0.5706969142357894	0.0
9	0.0	0.0	0.0	0.6226359671916307	0.0
10	0.0	0.0	0.0	0.699068320642309	0.0
11	0.0	0.0	0.0	0.808730822242809	0.0
12	0.0	0.0	0.0	0.8865188934330539	0.0
13	0.0	0.0	0.0	0.922339968818492	0.0
14	0.0	0.0	0.0	0.9364695603708642	0.0
15	0.0	0.0	0.0	0.9595468890043759	0.0
16	0.0	0.0	0.0	1.0155832222397962	0.0
17	0.0	0.0	0.0	1.081561485565372	0.0
18	0.0	0.0	0.0	1.1800468475796673	0.0
19	0.0	0.0	0.0	1.223881721158988	0.0
20	0.0	0.0	0.0	1.26645125818138	0.0
21	0.0	0.0	0.0	1.3469808919116373	0.0
22	0.0	0.0	0.0	1.4222684170489037	0.0
23	0.0	0.0	0.0	1.522019115620128	0.0
24	0.0	0.0	0.0	1.5892024613808737	0.0
25	0.0	0.0	0.0	1.644033712181124	0.0
26	0.0	0.0	0.0	1.7133259522034179	0.0
27	0.0	0.0	0.0	1.7670726288120147	0.0
28	0.0	0.0	0.0	1.825790270465689	0.0
29	0.0	0.0	0.0	1.8901416725037847	0.0
30	0.0	0.0	0.0	1.9981170586950463	0.0
31	0.0	0.0	0.0	2.1101294710441287	0.0
32	0.0	0.0	0.0	2.195208290967154	0.0
33	3.012706087925827E-5	0.0	0.0	2.277214150680495	0.0
34	3.012706087925827E-5	0.0	0.0	2.365516566117601	0.0
35	3.012706087925827E-5	0.0	0.0	2.520429913158747	0.0
36	3.012706087925827E-5	0.0	0.0	2.6319301654728817	0.0
37	3.012706087925827E-5	0.0	0.0	2.749305194658472	0.0
38	3.012706087925827E-5	0.0	0.0	2.848242462585956	0.0
39	3.012706087925827E-5	0.0	0.0	2.9444080409125486	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCAG	30	2.1667456E-6	45.000004	1
CAACGAC	30	2.1667456E-6	45.000004	13
ACGTTGA	60	0.0	45.000004	41
CGTTGAA	125	0.0	45.000004	2
GCTATCG	60	0.0	45.000004	1
TACGGTG	30	2.1667456E-6	45.000004	35
CCCCGAT	30	2.1667456E-6	45.000004	40
ACGGACG	125	0.0	45.000004	1
TTGCGTC	30	2.1667456E-6	45.000004	32
TCATCCG	30	2.1667456E-6	45.000004	1
AGTCGTG	30	2.1667456E-6	45.000004	1
CTTAACG	165	0.0	45.0	1
GTCATCG	45	3.8562575E-10	45.0	1
CGGAATA	25	3.8924034E-5	45.0	29
AGCGTCG	20	7.0352154E-4	45.0	1
TCGTTAG	345	0.0	45.0	1
CGTATAT	25	3.8924034E-5	45.0	23
GGTCGTA	50	2.1827873E-11	45.0	8
ACGTTAG	110	0.0	45.0	1
CCTAGCG	45	3.8562575E-10	45.0	1
>>END_MODULE