##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548931_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2584159
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95024222580731	31.0	31.0	33.0	30.0	33.0
2	31.260237856881098	31.0	31.0	33.0	30.0	34.0
3	31.398399634078245	31.0	31.0	34.0	30.0	34.0
4	35.26292383711684	37.0	35.0	37.0	33.0	37.0
5	35.1624752966052	37.0	35.0	37.0	32.0	37.0
6	35.24731179466898	37.0	35.0	37.0	32.0	37.0
7	35.5837609063529	36.0	35.0	37.0	35.0	37.0
8	35.64158203887609	37.0	35.0	37.0	35.0	37.0
9	37.49014476276421	39.0	37.0	39.0	35.0	39.0
10	37.06208209324581	39.0	37.0	39.0	33.0	39.0
11	36.63807567568404	39.0	35.0	39.0	32.0	39.0
12	35.23783172784647	35.0	35.0	39.0	31.0	39.0
13	34.777023008259164	35.0	34.0	39.0	30.0	39.0
14	35.43525688628293	36.0	33.0	40.0	29.0	41.0
15	35.85917043030247	36.0	34.0	40.0	31.0	41.0
16	36.046155441673676	35.0	34.0	40.0	32.0	41.0
17	35.90986816213708	35.0	34.0	40.0	31.0	41.0
18	36.00152893068886	36.0	35.0	40.0	31.0	41.0
19	35.89227017377801	36.0	35.0	40.0	31.0	41.0
20	35.60941877028465	35.0	34.0	40.0	31.0	41.0
21	35.477058106718665	35.0	34.0	40.0	30.0	41.0
22	35.3106325887842	35.0	34.0	40.0	30.0	41.0
23	35.26299774897752	35.0	34.0	40.0	30.0	41.0
24	35.351375824784775	35.0	34.0	39.0	30.0	41.0
25	35.2593226655171	35.0	34.0	39.0	30.0	41.0
26	35.03659914115192	35.0	34.0	39.0	30.0	41.0
27	35.00259271972042	35.0	34.0	39.0	29.0	41.0
28	35.09553204737015	36.0	34.0	39.0	29.0	41.0
29	35.098121284332734	36.0	34.0	39.0	29.0	41.0
30	35.05658591441161	36.0	34.0	39.0	29.0	41.0
31	34.80491099812357	35.0	34.0	39.0	29.0	41.0
32	34.66099454406637	35.0	34.0	39.0	29.0	41.0
33	34.42020982455027	35.0	33.0	40.0	27.0	41.0
34	34.213679189244935	35.0	34.0	39.0	26.0	41.0
35	34.00232145158251	35.0	33.0	39.0	25.0	41.0
36	33.723922947465695	35.0	33.0	39.0	23.0	41.0
37	33.73503913652372	35.0	33.0	39.0	23.0	41.0
38	33.80270718636121	35.0	33.0	39.0	24.0	41.0
39	33.7743281276423	35.0	33.0	39.0	23.0	41.0
40	33.46991884013329	35.0	33.0	39.0	23.0	41.0
41	33.59902467301741	35.0	33.0	39.0	23.0	41.0
42	33.57313578614938	35.0	33.0	39.0	23.0	41.0
43	33.54496491895429	35.0	33.0	39.0	23.0	41.0
44	33.50908864353935	35.0	33.0	39.0	23.0	41.0
45	33.56800220110295	35.0	33.0	39.0	23.0	41.0
46	33.57884286531905	35.0	33.0	39.0	23.0	41.0
47	33.54264927196817	35.0	33.0	39.0	23.0	41.0
48	33.568918553386226	35.0	33.0	39.0	23.0	41.0
49	33.626973804630445	35.0	33.0	39.0	24.0	41.0
50	33.42592812593962	35.0	33.0	39.0	24.0	41.0
51	33.10711918268187	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	3.0
11	7.0
12	9.0
13	8.0
14	23.0
15	55.0
16	158.0
17	525.0
18	1282.0
19	2792.0
20	4976.0
21	8097.0
22	12555.0
23	18439.0
24	26995.0
25	37952.0
26	48454.0
27	55649.0
28	61004.0
29	69027.0
30	82815.0
31	102269.0
32	132906.0
33	179408.0
34	334179.0
35	480763.0
36	144568.0
37	175789.0
38	252167.0
39	351151.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.886582830236065	24.996565613803178	29.039080025648577	14.077771530312184
2	30.470145219392457	26.860615000857145	30.6166532322508	12.052586547499592
3	28.721645997788837	25.468363208301035	33.28618711155158	12.523803682358556
4	26.055285297847387	27.76489372364471	33.00462548937585	13.175195489132054
5	21.41547791757396	35.094589767889666	31.604131169947358	11.885801144589013
6	20.209127998702865	42.1404023514033	28.76398085411927	8.886488795774564
7	84.87759460621425	5.8278921691738015	7.886511627187026	1.4080015974249263
8	87.06163204353912	4.4046051345911765	7.389676873597948	1.1440859482717587
9	82.33173732730842	7.37508798800693	8.223062125821205	2.0701125588634444
10	60.6169357226084	20.311791960169636	13.213931495701308	5.8573408215206575
11	58.53262125124654	15.961014782759111	18.423285873663346	7.083078092331006
12	51.287594919662446	21.865218045793622	19.571280250170364	7.275906784373562
13	17.717640439307335	53.526582536136516	19.404881820352387	9.350895204203766
14	9.543607804318542	53.82072078382174	26.542252237575166	10.093419174284554
15	10.355399957974722	23.051909731560634	57.60984521463269	8.982845095831951
16	10.180333330882503	17.52512132573886	55.81336132954667	16.481184013831964
17	10.158159772676527	19.314252722065476	30.19407861513165	40.33350889012634
18	15.285321065770333	24.24134892628511	45.0202948038414	15.453035204103152
19	34.74414693523115	18.999101835452077	27.669737040174386	18.587014189142387
20	32.77000370333249	22.05568620197132	29.762603616882704	15.411706477813478
21	15.659059678603368	36.56783502872695	31.03651903772175	16.736586254947934
22	18.847060107369554	25.387872805040246	22.1969313807703	33.568135706819895
23	13.568979308161765	34.35945698387754	26.221451543809803	25.85011216415089
24	10.657509851367506	22.20734095696124	55.06565192002505	12.069497271646211
25	9.490824674487909	26.01213779802249	49.40833749006931	15.088700037420297
26	9.397254580697242	44.00541917118877	29.283492230934705	17.313834017179282
27	10.727668073055877	51.12936162209834	26.530720439415685	11.612249865430108
28	8.870893780142787	36.310652711385025	42.96902783458758	11.849425673884618
29	9.193397155515585	27.59269069743773	41.558201333586666	21.655710813460008
30	13.817996493249835	44.838223963773125	29.047322552520953	12.296456990456083
31	33.82868469006745	30.926928257897444	25.090329194140143	10.154057857894967
32	36.159036653704355	23.270588226188867	29.662106704734498	10.908268415372275
33	35.4944490644732	29.23953208761535	21.117469938962735	14.148548908948714
34	18.733328715454427	28.59274526064379	24.512230091105074	28.161695932796704
35	20.231572438073663	24.141896841486922	35.163316189135415	20.463214531303993
36	38.18569213426883	27.916664570562418	22.45016657256771	11.447476722601047
37	15.604960840257895	46.08721831744873	27.661262329446444	10.646558512846926
38	16.055745795827576	44.461041290415956	22.36928919621432	17.11392371754215
39	20.850148926594684	40.08305990459565	26.495970255700215	12.570820913109449
40	28.003540029851102	25.58836356431628	24.331474959551638	22.076621446280974
41	12.910467196484426	25.731621003196786	28.080973345680356	33.27693845463843
42	15.62721179308239	22.385116395701658	27.036687757990123	34.950984053225824
43	17.85683466071554	21.756323817535993	29.478449274986563	30.908392246761906
44	11.74478814964559	32.83667916718747	34.49872859990426	20.919804083262676
45	11.129965300122786	53.81464530626792	21.543875589698622	13.511513803910672
46	18.112352993759284	44.767562677064376	22.466458139766168	14.65362618941017
47	18.370502743832713	30.347784327512358	25.11188359539796	26.16982933325697
48	17.41932288222203	29.066361628676873	39.85006340554123	13.664252083559875
49	21.77992917618459	28.84957930220238	37.461046321066156	11.90944520054687
50	18.672419150679197	45.8592524685981	23.66917051156682	11.799157869155884
51	17.350209487883678	39.53073320952774	24.38216843468223	18.736888867906348
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4181.0
1	5269.0
2	6357.0
3	15287.0
4	24217.0
5	19134.0
6	14051.0
7	14499.5
8	14948.0
9	16230.5
10	17513.0
11	17356.0
12	17199.0
13	16568.5
14	15938.0
15	15163.0
16	14388.0
17	14029.5
18	13671.0
19	13333.0
20	12995.0
21	13591.5
22	14188.0
23	15073.0
24	15958.0
25	18727.0
26	29256.0
27	37016.0
28	50385.5
29	63755.0
30	73139.0
31	82523.0
32	87434.0
33	92345.0
34	100883.5
35	109422.0
36	110850.0
37	112278.0
38	132238.0
39	152198.0
40	216743.5
41	281289.0
42	339210.0
43	397131.0
44	370095.0
45	343059.0
46	294622.0
47	246185.0
48	200567.5
49	154950.0
50	132576.5
51	110203.0
52	83861.5
53	57520.0
54	43781.5
55	30043.0
56	26849.0
57	23655.0
58	22404.0
59	21153.0
60	19692.5
61	18232.0
62	16027.0
63	13822.0
64	11362.5
65	8903.0
66	7627.0
67	6351.0
68	5387.5
69	4424.0
70	4279.5
71	4135.0
72	3547.0
73	2959.0
74	2325.0
75	1234.0
76	777.0
77	652.0
78	527.0
79	421.5
80	316.0
81	211.0
82	106.0
83	78.5
84	51.0
85	38.5
86	26.0
87	16.0
88	6.0
89	5.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2584159.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.667067354842908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.27232027281244	11.773595281708618
2	7.024728679172422	2.060643373738371
3	2.44398798489975	1.0753840916685429
4	1.4043701680667702	0.8239196738466948
5	0.9435773829991383	0.6919756515477381
6	0.7073596908283196	0.6224935337687891
7	0.5614922726197288	0.5764811487216164
8	0.47802947453875705	0.560903240052809
9	0.38738229166123633	0.5113585947481878
>10	3.804078723143176	11.40144775055989
>50	0.5277407789019133	5.536711384511897
>100	1.3248958201093828	49.55385344616056
>500	0.10417261087516108	9.925886882714774
>1k	0.014806259413728477	3.2703020288727824
>5k	5.287949790617314E-4	0.6580469237640043
>10k+	5.287949790617314E-4	0.9569969936147149
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12694	0.4912236437463794	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	11984	0.4637485541717828	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	9314	0.36042673844759554	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	7655	0.2962279023852635	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	3664	0.1417869411286225	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3402	0.13164824610250375	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2952	0.1142344569355059	No Hit
AGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	2783	0.10769461167056671	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2748	0.10634020584646689	No Hit
GAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2664	0.10308963186862728	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009093867676098879	0.0
2	0.0	0.0	0.0	0.03536934066363564	0.0
3	0.0	0.0	0.0	0.05096435629541371	0.0
4	0.0	0.0	0.0	0.07820726201445034	0.0
5	0.0	0.0	0.0	0.14132257341750257	0.0
6	0.0	0.0	0.0	0.2049022525316747	0.0
7	0.0	0.0	0.0	0.24990722320104916	0.0
8	0.0	0.0	0.0	0.3612006846327954	0.0
9	0.0	0.0	0.0	0.40461906562251004	0.0
10	0.0	0.0	0.0	0.478105255907241	0.0
11	0.0	0.0	0.0	0.5562738206124314	0.0
12	0.0	0.0	0.0	0.6174929638617438	0.0
13	0.0	0.0	0.0	0.6438071341585405	0.0
14	0.0	0.0	0.0	0.6541393157309593	0.0
15	0.0	0.0	0.0	0.6696569367442173	0.0
16	0.0	0.0	0.0	0.7072707213449327	0.0
17	0.0	0.0	0.0	0.7504956157883473	0.0
18	0.0	0.0	0.0	0.8133013487173196	0.0
19	0.0	0.0	0.0	0.8439109203419759	0.0
20	0.0	0.0	0.0	0.8765327520481518	0.0
21	0.0	0.0	0.0	0.9256396374990857	0.0
22	0.0	0.0	0.0	0.9779970969278593	0.0
23	0.0	0.0	0.0	1.0420024464438915	0.0
24	0.0	0.0	0.0	1.0890196771947855	0.0
25	0.0	0.0	0.0	1.1275621972177408	0.0
26	0.0	0.0	0.0	1.1748116118241951	0.0
27	0.0	0.0	0.0	1.2217901452658293	0.0
28	0.0	0.0	0.0	1.2740315127668227	0.0
29	0.0	0.0	0.0	1.3260019991029963	0.0
30	0.0	0.0	0.0	1.4006104113562672	0.0
31	0.0	0.0	0.0	1.4707299357353785	0.0
32	3.869730925999522E-5	0.0	0.0	1.531523408582831	0.0
33	3.869730925999522E-5	0.0	0.0	1.596225309665543	0.0
34	3.869730925999522E-5	0.0	0.0	1.6676992398687542	0.0
35	3.869730925999522E-5	0.0	0.0	1.7661451946261821	0.0
36	3.869730925999522E-5	0.0	0.0	1.847912609092552	0.0
37	3.869730925999522E-5	0.0	0.0	1.928673893518162	0.0
38	3.869730925999522E-5	0.0	0.0	2.009706059108592	0.0
39	3.869730925999522E-5	0.0	0.0	2.0874102561026624	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	25	3.8921615E-5	45.000004	36
TATGCGA	50	2.1827873E-11	45.000004	1
CGTTACC	25	3.8921615E-5	45.000004	44
CGCACAC	25	3.8921615E-5	45.000004	21
TAATGCG	65	0.0	45.000004	1
GTACGCG	100	0.0	45.000004	1
CGGCGTA	35	1.2127202E-7	45.0	21
CTCGTTA	40	6.8212103E-9	45.0	11
CTCCGTA	20	7.034924E-4	45.0	22
GATCACG	45	3.8562575E-10	45.0	1
TAAGTCG	35	1.2127202E-7	45.0	1
CACTACG	20	7.034924E-4	45.0	1
CGGTCTA	140	0.0	45.0	31
CGCGTCA	20	7.034924E-4	45.0	39
CGTCATT	20	7.034924E-4	45.0	23
TGTAACG	70	0.0	45.0	1
GCGTACA	35	1.2127202E-7	45.0	44
ACGTTAG	120	0.0	44.999996	1
TTAGCCG	30	2.1665583E-6	44.999996	24
TGAGTCG	30	2.1665583E-6	44.999996	16
>>END_MODULE