##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548927_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3839863
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.88790146940138	31.0	31.0	33.0	30.0	33.0
2	31.203150216557205	31.0	31.0	33.0	30.0	34.0
3	31.212526592745625	31.0	31.0	34.0	28.0	34.0
4	35.13854035938262	35.0	35.0	37.0	32.0	37.0
5	35.06745865672812	37.0	35.0	37.0	32.0	37.0
6	35.15885827176647	37.0	35.0	37.0	32.0	37.0
7	35.56859554624735	36.0	35.0	37.0	35.0	37.0
8	35.63708783360239	37.0	35.0	37.0	35.0	37.0
9	37.45857417308899	39.0	37.0	39.0	35.0	39.0
10	36.9460238555386	39.0	37.0	39.0	33.0	39.0
11	36.548523215541806	39.0	35.0	39.0	32.0	39.0
12	35.4879434500658	37.0	35.0	39.0	31.0	39.0
13	35.13255525001804	37.0	35.0	39.0	30.0	39.0
14	35.90486691842912	37.0	35.0	40.0	30.0	41.0
15	36.24398526718271	37.0	35.0	40.0	31.0	41.0
16	36.36516745519306	37.0	35.0	40.0	32.0	41.0
17	36.18756736893999	37.0	35.0	40.0	31.0	41.0
18	36.20764100177532	37.0	35.0	40.0	31.0	41.0
19	36.037064343180994	36.0	35.0	40.0	31.0	41.0
20	35.741531403594344	36.0	34.0	40.0	31.0	41.0
21	35.6104894888177	35.0	34.0	40.0	30.0	41.0
22	35.433044356009574	35.0	34.0	40.0	30.0	41.0
23	35.324538922352176	35.0	34.0	40.0	30.0	41.0
24	35.354583483837835	35.0	34.0	40.0	30.0	41.0
25	35.208182166915854	35.0	34.0	39.0	30.0	41.0
26	34.91988646469939	35.0	34.0	39.0	29.0	41.0
27	34.88304061889708	35.0	34.0	39.0	29.0	41.0
28	34.89097866251999	35.0	34.0	39.0	29.0	41.0
29	34.8272813379019	35.0	34.0	39.0	29.0	41.0
30	34.74211996625921	35.0	34.0	39.0	29.0	41.0
31	34.49649167170808	35.0	34.0	39.0	27.0	41.0
32	34.268912719021486	35.0	33.0	39.0	25.0	41.0
33	33.94798512342758	35.0	33.0	39.0	24.0	41.0
34	33.64970026274375	35.0	33.0	39.0	23.0	41.0
35	33.37328232804139	35.0	33.0	39.0	23.0	41.0
36	33.12935643797709	35.0	33.0	39.0	21.0	41.0
37	33.14349314025006	35.0	33.0	39.0	21.0	41.0
38	33.151148621708636	35.0	33.0	39.0	22.0	41.0
39	33.13195392648123	35.0	33.0	39.0	21.0	41.0
40	32.848898254963785	35.0	32.0	39.0	19.0	41.0
41	32.86200992066644	35.0	32.0	39.0	19.0	41.0
42	32.78628716701611	35.0	32.0	39.0	18.0	41.0
43	32.732448527460484	35.0	32.0	39.0	18.0	41.0
44	32.693325256656294	35.0	32.0	39.0	18.0	41.0
45	32.75060594609756	35.0	32.0	39.0	20.0	41.0
46	32.75535038619867	35.0	32.0	38.0	20.0	41.0
47	32.67514830607238	35.0	32.0	38.0	20.0	41.0
48	32.684240557540726	35.0	32.0	38.0	20.0	41.0
49	32.7022135945996	35.0	32.0	38.0	19.0	41.0
50	32.54817033836884	35.0	32.0	38.0	18.0	40.0
51	32.292416682574355	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	3.0
11	14.0
12	5.0
13	15.0
14	51.0
15	151.0
16	386.0
17	1149.0
18	2685.0
19	5606.0
20	9933.0
21	15776.0
22	24456.0
23	35947.0
24	51289.0
25	71254.0
26	90574.0
27	99478.0
28	102942.0
29	111045.0
30	127744.0
31	152889.0
32	192131.0
33	252402.0
34	437127.0
35	640240.0
36	294999.0
37	277935.0
38	368804.0
39	472654.0
40	176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.02227865942092	23.647197829714237	27.577650556803718	17.75287295406112
2	35.03807297291596	25.195846830993712	28.556201093632765	11.209879102457561
3	26.94119555827903	25.206055528543597	35.594499074576355	12.258249838601012
4	24.57509551773071	27.745443001482084	35.003592576089304	12.675868904697902
5	21.545169710481858	32.033252228009175	34.34195438743517	12.079623674073789
6	19.469132101848423	39.387004171763415	32.1387507835566	9.005112942831554
7	82.20840170599837	5.107005119713906	11.35412904054129	1.3304641337464385
8	84.04794650225801	3.617785322028416	11.052738079457521	1.2815300962560383
9	80.0612157256652	5.619575490063057	12.272859734839498	2.0463490494322323
10	53.08942011733231	21.819867010880337	17.451586163360517	7.639126708426837
11	48.4571975614755	19.96498312570006	22.567810361984268	9.010008950840174
12	43.23612587219909	23.669646547285673	23.557611300194825	9.536616280320418
13	17.408954434051424	48.352141730056516	23.59594600119848	10.642957834693581
14	10.665484680052387	48.66342887754068	29.210000460953943	11.461085981452984
15	10.400996077203796	26.179085035065054	53.07559149896755	10.34432738876361
16	11.148652959754033	21.084267850181114	51.54626610376464	16.220813086300215
17	11.228838112193065	22.582889024946983	32.144480154630514	34.04379270822944
18	15.849627968497835	26.88233929179244	41.793079596850205	15.474953142859524
19	29.56397142293879	24.665671665890162	29.07246951258417	16.69788739858688
20	29.957787556483133	25.317543881122845	29.921588348334303	14.803080214059722
21	16.41331995438379	36.09313665617758	31.42739727953836	16.066146109900277
22	17.970484884486766	28.425102666423257	25.021830205921407	28.58258224316857
23	13.656060125061753	36.164857964984684	28.42999867443187	21.749083235521685
24	11.901179807717098	25.17204389844117	50.31322732087056	12.61354897297117
25	11.307903433013104	29.588138951832395	44.309237074343535	14.79472054081096
26	10.326410082859727	43.111433923554046	30.421970783853485	16.140185209732742
27	11.113208986883127	47.77258980333413	28.333250431070066	12.780950778712677
28	10.143955656751295	36.159935914380284	41.724717782900065	11.97139064596836
29	10.444982021493995	30.39785534015146	39.85053633423901	19.306626304115536
30	14.2332421755672	42.67386622908161	30.08701091679573	13.00588067855546
31	28.668236340723613	33.232800232716635	27.12336351583377	10.975599910725983
32	31.216660594401414	27.52371634092154	29.547668757973916	11.71195430670313
33	30.46272744626566	31.770638692057503	22.865268891103668	14.90136497057317
34	17.517317675135804	30.983371021309875	26.143094167682545	25.356217135871773
35	18.154892505279484	29.782963610941326	33.69958771966604	18.362556164113148
36	31.813348549153968	32.2302905077603	23.197103646666562	12.759257296419168
37	15.35898546380431	45.730095058078895	27.494835102189846	11.416084375926953
38	15.671574741078	44.04607664388026	24.44063759566422	15.841711019377513
39	18.887314469292264	41.32262010389433	26.19249697189717	13.597568454916232
40	25.227280244112876	29.220365414078575	26.061580842858195	19.490773498950354
41	13.181876540907838	29.826558916294672	27.705702000305738	29.285862542491753
42	15.43328498959468	27.866775455270147	26.26106712661363	30.43887242852154
43	16.724555016676376	27.38259151433267	28.849024040701455	27.043829428289502
44	12.837489254173912	35.334411670416365	33.03128262648954	18.796816448920183
45	12.386483580273568	51.10122418430032	22.206130791645432	14.306161443780677
46	16.499937627983083	44.48726425916758	23.96663630968084	15.046161803168498
47	16.666480028063503	33.617162903989026	25.98764591340889	23.728711154538587
48	17.054566790533933	31.32460715395315	36.79813576682293	14.822690288689987
49	19.591922941000757	31.958536020686157	35.0415366381561	13.408004400156983
50	17.28238741850946	44.78099869708893	24.535771198087016	13.40084268631459
51	15.511048180625195	41.547471875949746	24.518218488524198	18.423261454900867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6146.0
1	7796.0
2	9446.0
3	50281.0
4	91116.0
5	63648.0
6	36180.0
7	40750.5
8	45321.0
9	54432.0
10	63543.0
11	69987.5
12	76432.0
13	73928.0
14	71424.0
15	64438.0
16	57452.0
17	49873.5
18	42295.0
19	39551.5
20	36808.0
21	33694.0
22	30580.0
23	31177.5
24	31775.0
25	34304.0
26	41296.0
27	45759.0
28	59475.5
29	73192.0
30	84107.0
31	95022.0
32	102600.0
33	110178.0
34	127223.5
35	144269.0
36	146603.5
37	148938.0
38	170580.5
39	192223.0
40	275038.5
41	357854.0
42	414635.5
43	471417.0
44	455237.5
45	439058.0
46	378304.0
47	317550.0
48	273439.5
49	229329.0
50	195155.0
51	160981.0
52	130949.5
53	100918.0
54	83710.0
55	66502.0
56	61240.5
57	55979.0
58	52624.5
59	49270.0
60	45894.5
61	42519.0
62	37970.0
63	33421.0
64	27926.5
65	22432.0
66	18115.5
67	13799.0
68	12555.0
69	11311.0
70	9955.5
71	8600.0
72	7520.5
73	6441.0
74	5067.5
75	2761.5
76	1829.0
77	1502.0
78	1175.0
79	799.5
80	424.0
81	332.5
82	241.0
83	180.0
84	119.0
85	80.0
86	41.0
87	26.0
88	11.0
89	9.5
90	8.0
91	6.0
92	4.0
93	3.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3839863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.687817930271624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.39272445542059	13.082035127700106
2	6.785258933688031	2.2646233139026966
3	2.584375435095136	1.2938276017300239
4	1.5202516847452112	1.014787332928671
5	1.1384324480974342	0.9498976709881689
6	0.9010021723992506	0.9021456124666739
7	0.7324275383507004	0.8555832184979467
8	0.6265335684224258	0.8364382493629455
9	0.5585726408052206	0.8389222677529679
>10	4.948556468155508	17.312356076438924
>50	0.7138768805361119	8.309308793450096
>100	1.0146313591271447	39.94612198986463
>500	0.07295655995813469	7.918969340161515
>1k	0.010084708071966781	2.595374674164347
>5k	1.5757356362448095E-4	0.16915952663525072
>10k+	1.5757356362448095E-4	1.7104492039550994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	65047	1.6939927283864034	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6433	0.16753201872045956	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4496	0.11708751067420896	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4233	0.11023830798130038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01679747428488985	0.0
2	0.0	0.0	0.0	0.058361457166570783	0.0
3	0.0	0.0	0.0	0.09148763901212101	0.0
4	0.0	0.0	0.0	0.13237451440324824	0.0
5	0.0	0.0	0.0	0.2500349621848488	0.0
6	0.0	0.0	0.0	0.40501444973427436	0.0
7	0.0	0.0	0.0	0.5397848829502511	0.0
8	0.0	0.0	0.0	0.7408337224531187	0.0
9	0.0	0.0	0.0	0.8580774887020709	0.0
10	0.0	0.0	0.0	0.9895925974442318	0.0
11	0.0	0.0	0.0	1.1075915989711091	0.0
12	0.0	0.0	0.0	1.1915268852039773	0.0
13	0.0	0.0	0.0	1.2305386936981866	0.0
14	0.0	0.0	0.0	1.2463986345346174	0.0
15	0.0	0.0	0.0	1.2673629241459916	0.0
16	0.0	0.0	0.0	1.3080154161750042	0.0
17	0.0	0.0	0.0	1.3589286909454843	0.0
18	0.0	0.0	0.0	1.4299989348578321	0.0
19	0.0	0.0	0.0	1.465182481770834	0.0
20	0.0	0.0	0.0	1.5000535175343495	0.0
21	0.0	0.0	0.0	1.5535189666923013	0.0
22	0.0	0.0	0.0	1.6082344604481982	0.0
23	0.0	0.0	0.0	1.6743826537561366	0.0
24	0.0	0.0	0.0	1.724514650652901	0.0
25	2.60425957905269E-5	0.0	0.0	1.7645160777871502	0.0
26	2.60425957905269E-5	0.0	0.0	1.8067571681593848	0.0
27	2.60425957905269E-5	0.0	0.0	1.8506128994706321	0.0
28	2.60425957905269E-5	0.0	0.0	1.8976979126599047	0.0
29	2.60425957905269E-5	0.0	0.0	1.947777824365088	0.0
30	2.60425957905269E-5	0.0	0.0	2.026061867311412	0.0
31	2.60425957905269E-5	0.0	0.0	2.09614249258372	0.0
32	2.60425957905269E-5	0.0	0.0	2.158332211331498	0.0
33	2.60425957905269E-5	0.0	0.0	2.2166936684980687	0.0
34	2.60425957905269E-5	0.0	0.0	2.2813313912501565	0.0
35	2.60425957905269E-5	0.0	0.0	2.380892234957341	0.0
36	2.60425957905269E-5	0.0	0.0	2.4557907404508965	0.0
37	2.60425957905269E-5	0.0	0.0	2.5338404000351056	0.0
38	2.60425957905269E-5	0.0	0.0	2.605275240288521	0.0
39	2.60425957905269E-5	0.0	0.0	2.682205068253737	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCG	25	3.892518E-5	45.0	12
ACCGGTC	20	7.035353E-4	45.0	20
CGTACTA	20	7.035353E-4	45.0	35
GTTCACG	90	0.0	45.0	1
GCTCGAC	20	7.035353E-4	45.0	20
CGTTTTT	29675	0.0	43.89301	1
AATGTCG	105	0.0	42.857147	1
ACGGGTA	405	0.0	42.77778	5
CATCGGC	90	0.0	42.5	4
TATCGTG	150	0.0	42.0	1
CGGTCTA	290	0.0	41.896553	31
CGAACGG	215	0.0	41.860466	2
GCGTAAG	345	0.0	41.739132	1
ACTACCG	60	3.6379788E-12	41.250004	1
CGACGGT	295	0.0	41.186436	28
ATTAGCG	210	0.0	40.714287	1
CGGGTAC	155	0.0	40.64516	6
ATACGTG	95	0.0	40.263157	1
CTCGATT	45	1.9303116E-8	40.0	24
TCGTACA	170	0.0	39.705883	34
>>END_MODULE