##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548925_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3499710
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.872421429204135	31.0	31.0	33.0	30.0	33.0
2	31.189622282989163	31.0	31.0	33.0	30.0	34.0
3	31.32871923673676	31.0	31.0	34.0	28.0	34.0
4	35.181589617425445	37.0	35.0	37.0	32.0	37.0
5	35.05105080135211	37.0	35.0	37.0	32.0	37.0
6	35.1266296350269	37.0	35.0	37.0	32.0	37.0
7	35.55537315949036	36.0	35.0	37.0	35.0	37.0
8	35.616005611893556	37.0	35.0	37.0	35.0	37.0
9	37.40556417531738	39.0	37.0	39.0	35.0	39.0
10	36.94993670904161	39.0	37.0	39.0	33.0	39.0
11	36.54953296130251	39.0	35.0	39.0	32.0	39.0
12	35.194197519223025	35.0	35.0	39.0	31.0	39.0
13	34.722414428624084	35.0	33.0	39.0	29.0	39.0
14	35.39795868800558	36.0	33.0	40.0	27.0	41.0
15	35.83398110129125	36.0	34.0	40.0	30.0	41.0
16	36.004814113169374	36.0	34.0	40.0	31.0	41.0
17	35.861172211411805	36.0	34.0	40.0	31.0	41.0
18	35.93686962634047	36.0	35.0	40.0	31.0	41.0
19	35.822082115375274	36.0	35.0	40.0	31.0	41.0
20	35.535354929408435	35.0	34.0	40.0	30.0	41.0
21	35.39322515294124	35.0	34.0	40.0	30.0	41.0
22	35.22225812995934	35.0	34.0	40.0	29.0	41.0
23	35.164588780213215	35.0	34.0	40.0	29.0	41.0
24	35.273987273231214	35.0	34.0	40.0	30.0	41.0
25	35.16713984873032	35.0	34.0	39.0	30.0	41.0
26	34.89021690368631	35.0	34.0	39.0	29.0	41.0
27	34.88213451971735	35.0	34.0	39.0	29.0	41.0
28	34.959552077172106	36.0	34.0	39.0	29.0	41.0
29	34.96289121098605	36.0	34.0	39.0	29.0	41.0
30	34.92707424329444	36.0	34.0	39.0	29.0	41.0
31	34.69760380145784	35.0	34.0	39.0	29.0	41.0
32	34.52884496143966	35.0	34.0	39.0	27.0	41.0
33	34.27897568655688	35.0	33.0	40.0	26.0	41.0
34	34.07853479288284	35.0	33.0	39.0	25.0	41.0
35	33.87344208520135	35.0	33.0	39.0	24.0	41.0
36	33.61579959482357	35.0	33.0	39.0	23.0	41.0
37	33.66273005477597	35.0	33.0	39.0	23.0	41.0
38	33.71012883924668	35.0	33.0	39.0	23.0	41.0
39	33.7085167056699	35.0	33.0	39.0	23.0	41.0
40	33.41181812207297	35.0	32.0	39.0	23.0	41.0
41	33.48763783284901	35.0	33.0	39.0	23.0	41.0
42	33.441564586780046	35.0	33.0	39.0	23.0	41.0
43	33.4139351546271	35.0	33.0	39.0	23.0	41.0
44	33.3883298901909	35.0	33.0	39.0	23.0	41.0
45	33.44651699712262	35.0	33.0	39.0	23.0	41.0
46	33.458475988010434	35.0	33.0	39.0	23.0	41.0
47	33.411292078486504	35.0	33.0	39.0	23.0	41.0
48	33.43879207134305	35.0	33.0	39.0	23.0	41.0
49	33.49458012235299	35.0	33.0	39.0	24.0	41.0
50	33.28250969366033	35.0	32.0	39.0	23.0	41.0
51	32.98823531092576	35.0	32.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	3.0
11	4.0
12	8.0
13	10.0
14	21.0
15	101.0
16	295.0
17	938.0
18	2207.0
19	4509.0
20	8083.0
21	13130.0
22	19746.0
23	28193.0
24	39463.0
25	54412.0
26	69206.0
27	80095.0
28	87658.0
29	99115.0
30	117213.0
31	142872.0
32	181758.0
33	239553.0
34	427186.0
35	620331.0
36	200694.0
37	240816.0
38	344165.0
39	477742.0
40	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.76748644887719	24.30192787402385	29.321229473299216	13.609356203799743
2	30.73300359172617	27.997234056536115	29.160273279786043	12.109489071951677
3	28.05409591080404	26.27869166302351	33.58512562469462	12.082086801477837
4	27.15845027159393	28.42866980406948	31.53707021438919	12.875809709947394
5	22.838578053610156	33.515291266990694	30.932305819625057	12.713824859774094
6	20.68359949824414	42.17692323078197	28.23202493920925	8.907452331764631
7	84.5088307316892	5.799109069037149	8.136274148429441	1.5557860508442127
8	85.90106037357381	5.024530603964329	7.691380142926128	1.383028879535733
9	81.00408319546477	7.782673421512068	9.10898331576045	2.104260067262716
10	57.75927148249427	21.915444422537867	13.66818964999957	6.657094444968298
11	56.4047021038886	16.961205357015295	19.169474042134922	7.464618496961177
12	49.52624646042101	22.568784270696714	20.25522114689503	7.64974812198725
13	17.68049352660649	52.9141557443331	19.97656948718608	9.428781241874326
14	10.28490932105803	52.505036131565184	27.146792162779203	10.06326238459758
15	10.114752365195974	23.563895294181517	57.17319435038903	9.148157990233477
16	10.431550042717825	18.03729451868869	54.84337273659817	16.687782701995307
17	10.514414051449977	19.26416760245849	31.339082381111577	38.88233596497996
18	16.022156121507212	24.057964802797947	43.865463138374324	16.05441593732052
19	34.225921576359184	19.78158190250049	28.63400110294853	17.358495418191794
20	33.17666320923734	22.17389440839384	29.71729086124279	14.932151521126036
21	17.221398344434252	35.8115958179392	30.10169414037163	16.865311697254914
22	18.270285252206612	26.37189938594912	22.211354655099992	33.14646070674427
23	13.788056724700045	35.002443059567796	26.68409668229653	24.52540353343563
24	11.301250675055934	22.55255435450366	53.65521714656357	12.490977823876834
25	10.351314823228211	26.968577396412847	47.390412348451726	15.289695431907216
26	9.647913684276697	43.784942180923565	29.61656822993905	16.95057590486069
27	11.194899005917632	49.62274016989979	27.761100205445594	11.42126061873698
28	9.470327541424862	35.20528843818488	43.478631086575746	11.845752933814516
29	10.126181883641786	27.72012538181735	41.84681016427076	20.30688257027011
30	14.547662520608851	43.45620065662584	29.490643510462295	12.50549331230302
31	33.374422452146035	30.778264484771594	25.10973766397787	10.737575399104497
32	36.20797151764003	23.940526500767206	28.867534738592624	10.983967243000134
33	35.03044537976004	28.936940489354836	21.175383103171406	14.857231027713723
34	18.67546168111072	27.63826145594921	25.447594229236138	28.238682633703938
35	20.056261804549518	25.055590320340826	35.227918884707584	19.66022899040206
36	36.3934726020156	28.08938454900549	23.462858351120524	12.054284497858395
37	16.027842306934005	46.20639995885373	26.84685302496493	10.918904709247338
38	15.633695363330105	44.78651088233025	22.744998871335053	16.83479488300459
39	20.14101168382523	40.67579885190487	25.999582822576727	13.183606641693169
40	27.3606670266965	26.371413631415173	24.73264927665435	21.535270065233977
41	13.759111469236021	25.80553817316292	27.25174371590789	33.18360664169317
42	15.443622471576216	23.463515548431154	26.12236442448089	34.97049755551174
43	17.464475627980605	22.86260861614248	28.911052630075062	30.761863125801852
44	12.15877887024925	33.15680442093773	34.619068437099074	20.065348271713944
45	12.007880652968389	52.204754108197534	21.794720133953955	13.992645104880118
46	17.139048664032163	45.03393138288601	22.79960339570993	15.027416557371897
47	18.164876518340094	30.378202765371988	25.718188078440786	25.738732637847132
48	17.648005120424266	28.076497766957836	39.823813973157776	14.451683139460128
49	21.266104905835054	28.96219972512008	37.558883450343025	12.212811918701835
50	17.658920310540015	45.16462792631389	24.257724211434663	12.918727551711429
51	16.658151675424534	40.38006006211943	24.90223475659412	18.059553505861913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5640.0
1	7594.5
2	9549.0
3	20739.0
4	31929.0
5	25714.0
6	19499.0
7	19959.5
8	20420.0
9	21695.5
10	22971.0
11	22540.0
12	22109.0
13	21429.0
14	20749.0
15	19352.0
16	17955.0
17	17159.5
18	16364.0
19	16147.0
20	15930.0
21	19225.5
22	22521.0
23	24273.5
24	26026.0
25	30832.5
26	48123.0
27	60607.0
28	70460.5
29	80314.0
30	95032.5
31	109751.0
32	118323.5
33	126896.0
34	137865.0
35	148834.0
36	151197.5
37	153561.0
38	183207.0
39	212853.0
40	294747.0
41	376641.0
42	454949.5
43	533258.0
44	501070.5
45	468883.0
46	395648.5
47	322414.0
48	267021.0
49	211628.0
50	178930.0
51	146232.0
52	113381.0
53	80530.0
54	61866.0
55	43202.0
56	37140.5
57	31079.0
58	29704.5
59	28330.0
60	26453.0
61	24576.0
62	21105.0
63	17634.0
64	14706.0
65	11778.0
66	9695.0
67	7612.0
68	6413.0
69	5214.0
70	4602.0
71	3990.0
72	3417.5
73	2845.0
74	2392.5
75	1402.5
76	865.0
77	668.5
78	472.0
79	364.5
80	257.0
81	187.5
82	118.0
83	85.0
84	52.0
85	37.5
86	23.0
87	17.5
88	12.0
89	9.0
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3499710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.801204676544947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.66740587078944	11.939747850194777
2	7.748282714746867	2.293678367054074
3	2.5572289700032926	1.1355020816942685
4	1.3148475429122124	0.7784531040438348
5	0.8378104927626879	0.6200302291768761
6	0.6027862399081817	0.5353177507851555
7	0.49649326943333427	0.514408895098683
8	0.4018999150166035	0.4758882321317414
9	0.3166467920009118	0.42180785807191656
>10	3.2878883409643422	10.087596823735494
>50	0.47984511664405743	5.029429201022636
>100	1.0792569855976801	42.64817457124321
>500	0.184788330256287	16.956495475158352
>1k	0.02365600870017525	4.9782941111237085
>5k	5.817051319715226E-4	0.50471449050923
>10k+	5.817051319715226E-4	1.0804609589560434
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15566	0.44477971031885494	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	11637	0.33251326538484616	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	10444	0.2984247266202057	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6931	0.1980449808698435	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5577	0.15935606093076285	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	5078	0.14509773666960976	No Hit
TAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4098	0.11709541647736527	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4036	0.11532384111826409	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	3970	0.11343797057470477	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3688	0.10538016007040583	No Hit
AGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA	3637	0.10392289646856454	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02091601875583977	0.0
2	0.0	0.0	0.0	0.06637692837406528	0.0
3	0.0	0.0	0.0	0.0967508736438162	0.0
4	0.0	0.0	0.0	0.13526835080620966	0.0
5	0.0	0.0	0.0	0.24393449742978704	0.0
6	0.0	0.0	0.0	0.33605641610304854	0.0
7	0.0	0.0	0.0	0.41106263090370343	0.0
8	0.0	0.0	0.0	0.5845341471150467	0.0
9	0.0	0.0	0.0	0.65379702889668	0.0
10	0.0	0.0	0.0	0.7600058290544074	0.0
11	0.0	0.0	0.0	0.8844161373370937	0.0
12	0.0	0.0	0.0	0.986195999097068	0.0
13	0.0	0.0	0.0	1.0428006891999624	0.0
14	0.0	0.0	0.0	1.0600021144609124	0.0
15	0.0	0.0	0.0	1.0928334061965135	0.0
16	0.0	0.0	0.0	1.1597818104928694	0.0
17	0.0	0.0	0.0	1.2381597332350396	0.0
18	0.0	0.0	0.0	1.3592840549645542	0.0
19	0.0	0.0	0.0	1.408916738815502	0.0
20	0.0	0.0	0.0	1.4629212134719733	0.0
21	0.0	0.0	0.0	1.540956250660769	0.0
22	2.8573796114535205E-5	0.0	0.0	1.626220458266542	0.0
23	2.8573796114535205E-5	0.0	0.0	1.7250286452306047	0.0
24	2.8573796114535205E-5	0.0	0.0	1.800577762157436	0.0
25	2.8573796114535205E-5	0.0	0.0	1.8616399644541977	0.0
26	2.8573796114535205E-5	0.0	0.0	1.9289884018961572	0.0
27	2.8573796114535205E-5	0.0	0.0	1.9887647833677649	0.0
28	2.8573796114535205E-5	0.0	0.0	2.058056238945513	0.0
29	2.8573796114535205E-5	0.0	0.0	2.1312908783870665	0.0
30	2.8573796114535205E-5	0.0	0.0	2.2450717345151454	0.0
31	2.8573796114535205E-5	0.0	0.0	2.3490232047798245	0.0
32	2.8573796114535205E-5	0.0	0.0	2.444173945841227	0.0
33	2.8573796114535205E-5	0.0	0.0	2.533381337310806	0.0
34	2.8573796114535205E-5	0.0	0.0	2.634504001760146	0.0
35	2.8573796114535205E-5	0.0	0.0	2.7788588197307775	0.0
36	5.714759222907041E-5	0.0	0.0	2.8907823791114122	0.0
37	5.714759222907041E-5	0.0	0.0	2.998248426298179	0.0
38	5.714759222907041E-5	0.0	0.0	3.101171239902735	0.0
39	5.714759222907041E-5	0.0	0.0	3.2006651979735463	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	30	2.1667802E-6	45.000004	6
CGACGAA	30	2.1667802E-6	45.000004	32
CGGCATA	20	7.0352654E-4	45.000004	26
CAACCGT	35	1.212884E-7	45.000004	20
TTCGTCA	20	7.0352654E-4	45.000004	13
CGCGATT	20	7.0352654E-4	45.000004	28
TACGACA	20	7.0352654E-4	45.000004	30
TCGTCAA	20	7.0352654E-4	45.000004	14
ACTAACG	35	1.212884E-7	45.000004	26
TAGTCCG	35	1.212884E-7	45.000004	1
GCGTAAA	40	6.8212103E-9	45.000004	1
AACCCGT	25	3.8924467E-5	45.0	44
CGCATTA	25	3.8924467E-5	45.0	14
CGTTCGA	25	3.8924467E-5	45.0	14
CAAGTCG	65	0.0	44.999996	1
GCAACCG	115	0.0	44.999996	1
TGTAGCG	170	0.0	43.676468	1
AGTACGG	450	0.0	43.5	2
CGAGGGT	410	0.0	43.35366	4
TAGTACG	265	0.0	43.301888	1
>>END_MODULE