##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548922_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	929047
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.993365244169564	31.0	31.0	33.0	30.0	33.0
2	31.313541726091362	31.0	31.0	33.0	30.0	34.0
3	31.47178990944484	31.0	31.0	34.0	30.0	34.0
4	35.31231035674191	37.0	35.0	37.0	33.0	37.0
5	35.20802069217166	37.0	35.0	37.0	32.0	37.0
6	35.269707560543225	37.0	35.0	37.0	32.0	37.0
7	35.64182866959368	36.0	35.0	37.0	35.0	37.0
8	35.72097105959117	37.0	35.0	37.0	35.0	37.0
9	37.57536916862118	39.0	37.0	39.0	35.0	39.0
10	37.104795559320465	39.0	37.0	39.0	33.0	39.0
11	36.65388295748224	39.0	35.0	39.0	32.0	39.0
12	35.10186459888466	35.0	35.0	39.0	31.0	39.0
13	34.669520487122824	35.0	34.0	39.0	30.0	39.0
14	35.262007196621916	35.0	33.0	40.0	29.0	41.0
15	35.7102956039899	35.0	34.0	40.0	31.0	41.0
16	35.91517113773577	35.0	34.0	40.0	32.0	41.0
17	35.790237738241444	35.0	34.0	40.0	31.0	41.0
18	35.923463506152004	36.0	35.0	39.0	31.0	41.0
19	35.72824625664794	36.0	35.0	39.0	31.0	41.0
20	35.40287197526067	35.0	34.0	39.0	30.0	41.0
21	35.32051984452885	35.0	34.0	39.0	30.0	41.0
22	35.149797588281324	35.0	34.0	39.0	30.0	41.0
23	35.10794071774625	35.0	34.0	39.0	30.0	41.0
24	35.235171094680894	35.0	34.0	39.0	31.0	41.0
25	35.14388723067832	35.0	34.0	39.0	30.0	41.0
26	34.91625719689101	35.0	34.0	39.0	30.0	41.0
27	34.89874893304644	35.0	34.0	39.0	29.0	41.0
28	35.04569521240583	35.0	34.0	39.0	30.0	41.0
29	35.07687555096782	36.0	34.0	39.0	30.0	41.0
30	35.03863959519809	36.0	34.0	39.0	30.0	41.0
31	34.730803716066035	35.0	34.0	39.0	29.0	41.0
32	34.53345740312385	35.0	34.0	39.0	29.0	41.0
33	34.33066895431555	35.0	33.0	39.0	27.0	41.0
34	34.1941042810536	35.0	34.0	39.0	27.0	41.0
35	33.984590661182914	35.0	33.0	39.0	26.0	41.0
36	33.70394070482979	35.0	33.0	39.0	23.0	41.0
37	33.70672635507138	35.0	33.0	39.0	24.0	41.0
38	33.768559610008964	35.0	33.0	39.0	24.0	41.0
39	33.776730348410794	35.0	33.0	39.0	24.0	41.0
40	33.406594068976055	35.0	33.0	39.0	23.0	41.0
41	33.58949009038294	35.0	33.0	39.0	23.0	41.0
42	33.61979964415148	35.0	33.0	39.0	23.0	41.0
43	33.5919119269531	35.0	33.0	39.0	23.0	41.0
44	33.5629037067016	35.0	33.0	39.0	23.0	41.0
45	33.64846773091136	35.0	33.0	39.0	23.0	41.0
46	33.68809974091731	35.0	33.0	39.0	24.0	41.0
47	33.63694624706823	35.0	33.0	38.0	24.0	41.0
48	33.66927399797857	35.0	33.0	38.0	23.0	41.0
49	33.73677973234938	35.0	33.0	38.0	24.0	41.0
50	33.496681007527066	35.0	33.0	38.0	24.0	41.0
51	33.21875965370966	35.0	32.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	4.0
13	1.0
14	8.0
15	19.0
16	57.0
17	171.0
18	440.0
19	889.0
20	1670.0
21	2747.0
22	4321.0
23	6419.0
24	9178.0
25	13051.0
26	16773.0
27	19580.0
28	21198.0
29	24747.0
30	29569.0
31	37227.0
32	48088.0
33	64908.0
34	124909.0
35	192591.0
36	47073.0
37	57256.0
38	83918.0
39	122173.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.974859183658094	24.691323474485145	30.983793069672473	13.35002427218429
2	30.011183503095108	26.69811107511245	31.566540767044078	11.72416465474836
3	27.57739920585288	26.7051074918707	33.6950660192649	12.022427283011517
4	25.340052763746073	27.87458546230707	33.32630103751479	13.459060736432063
5	22.563444045349698	32.334209141195224	32.39244085606003	12.709905957395051
6	20.10027479772283	41.920161197442106	29.070434542062994	8.909129462772066
7	86.92423526473902	5.175949117751847	6.77263905916493	1.1271765583441957
8	89.86014701086167	3.055711928460024	6.091833889996954	0.9923071706813542
9	86.07928339470446	5.244836913525365	7.212229305944693	1.4636503858254748
10	65.36278573635134	17.93364598346478	11.048848981806088	5.6547192983778
11	62.548504004641316	14.598615570579316	17.00753567903454	5.845344745744834
12	55.026925440801165	21.58007076068272	16.589580505614894	6.80342329290122
13	16.012322304468988	57.64057146732081	18.072175035278086	8.274931192932113
14	8.853588677429668	55.76004227988466	26.284461388928655	9.10190765375702
15	8.504413662602646	21.676298400403855	61.58816507668611	8.231122860307389
16	8.464480268490183	15.244008107232466	59.251900065335775	17.03961155894158
17	8.721410219289229	16.66664872713652	30.575094693809895	44.03684635976436
18	15.69942101960396	22.50079920606815	46.29249112262351	15.507288651704382
19	36.49277162511692	17.181907912086256	28.002027884488083	18.323292578308738
20	34.93084849313329	20.45784551265975	28.1847958176497	16.42651017655727
21	15.37188107813706	36.290844273755795	31.40131769436853	16.935956953738614
22	19.2793260190281	24.62900154674629	20.79012149008608	35.30155094413953
23	11.87378033619397	35.406174283970564	26.51749588556876	26.20254949426671
24	9.877756453656273	19.401709493706992	59.21336595457496	11.507168098061777
25	8.658657742826788	25.819253493095612	50.821540783189654	14.700547980887944
26	8.326704676943148	45.515027765010814	28.54085961205407	17.617407945991967
27	8.940344245231941	53.754438688247205	26.5025343174242	10.802682749096656
28	7.44440270513763	35.8826840837977	46.519067388409844	10.153845822654828
29	8.315940958853535	25.922800461117685	43.38854761922701	22.37271096080177
30	13.878415193203358	45.72395153313018	28.630413746559647	11.767219527106809
31	36.4406752295632	29.75812849080832	24.81413749788762	8.987058781740858
32	39.14226083287498	21.33853292675182	29.445227205943297	10.073979034429906
33	37.848246644141796	27.076240491600533	19.927086573660965	15.148426290596708
34	18.83101716059575	26.027854349672296	25.01122117610842	30.129907313623534
35	19.001837366677897	23.319702878325856	37.419743026994325	20.25871672800192
36	41.34440991682875	25.787069976007672	21.676836586308333	11.191683520855241
37	14.880947896069843	47.74936036605252	27.534451970675327	9.835239767202305
38	15.224418140309371	45.74827753601271	21.68383300306658	17.343471320611336
39	20.407363674819464	41.09662912640587	25.88189833237716	12.614108866397503
40	29.4950632206982	23.886520272924834	23.178913445713725	23.439503060663238
41	12.025333486895711	23.119497721859066	27.305184775366588	37.549984015878636
42	15.22022029025442	20.00695336188589	26.042708280635964	38.73011806722372
43	16.219631514874923	19.845497590541704	30.461860379507172	33.473010515076204
44	10.7236770583189	31.990738896955694	36.11744077533214	21.168143269393262
45	9.650211453241871	56.97957153943772	20.155923220246123	13.214293787074283
46	17.875521905780868	44.632725793205296	22.502736675324282	14.98901562568955
47	17.37113407610164	29.194647848817123	25.52088322765156	27.913334847429677
48	17.83644960911558	26.55753691686212	42.17246274946262	13.433550724559684
49	22.37540189032417	27.23705043985934	39.09242481812007	11.295122851696416
50	17.949038100332924	47.942676742941956	22.641481001499386	11.46680415522573
51	15.517406546708617	41.119448208755855	24.513183940102063	18.849961304433467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1073.0
1	1501.5
2	1930.0
3	4194.0
4	6458.0
5	5228.5
6	3999.0
7	4330.5
8	4662.0
9	4950.0
10	5238.0
11	5244.5
12	5251.0
13	5117.5
14	4984.0
15	4620.5
16	4257.0
17	4030.5
18	3804.0
19	3854.0
20	3904.0
21	4064.5
22	4225.0
23	4665.0
24	5105.0
25	6378.5
26	9129.0
27	10606.0
28	14060.5
29	17515.0
30	20560.0
31	23605.0
32	27827.5
33	32050.0
34	33102.0
35	34154.0
36	34601.5
37	35049.0
38	46168.5
39	57288.0
40	83675.0
41	110062.0
42	134385.0
43	158708.0
44	150855.5
45	143003.0
46	118343.0
47	93683.0
48	77823.5
49	61964.0
50	49778.0
51	37592.0
52	28561.0
53	19530.0
54	14128.5
55	8727.0
56	7120.5
57	5514.0
58	4990.5
59	4467.0
60	4227.5
61	3988.0
62	3469.5
63	2951.0
64	2493.0
65	2035.0
66	1675.5
67	1316.0
68	1097.0
69	878.0
70	824.5
71	771.0
72	606.0
73	441.0
74	361.5
75	216.5
76	151.0
77	122.0
78	93.0
79	69.0
80	45.0
81	28.0
82	11.0
83	9.5
84	8.0
85	9.5
86	11.0
87	8.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	929047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.334831128036093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.3034757149323	13.747123593846627
2	6.722009782042816	2.3304980882543784
3	2.690318428783152	1.3990864693079803
4	1.5795183642446624	1.0952273643125212
5	1.0942628484161916	0.948443084348922
6	0.8636296852341118	0.8982524850415375
7	0.6216362973495678	0.7543172166328671
8	0.5175093059166621	0.7176749140202004
9	0.4174490763482803	0.6512768318746877
>10	3.324574780671758	12.309761131761325
>50	1.1437598519633976	14.581703304046506
>100	1.7075588659475287	48.34861063674269
>500	0.010567346458357477	1.158579649616902
>1k	0.0037296516911849917	1.0594452301928368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3206	0.34508480195296903	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2206	0.23744762105684641	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1233	0.13271664404491915	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1140	0.12270638622157976	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	1044	0.11237321685555197	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1003	0.10796009243881094	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02281908234997799	0.0
2	0.0	0.0	0.0	0.08040497412940358	0.0
3	0.0	0.0	0.0	0.11129684504659075	0.0
4	0.0	0.0	0.0	0.15876484182178083	0.0
5	0.0	0.0	0.0	0.278349749797373	0.0
6	0.0	0.0	0.0	0.38577165633170335	0.0
7	0.0	0.0	0.0	0.46283987785332714	0.0
8	0.0	0.0	0.0	0.6482987405373464	0.0
9	1.0763718089612258E-4	0.0	0.0	0.7162178016827997	0.0
10	1.0763718089612258E-4	0.0	0.0	0.8331117801359889	0.0
11	1.0763718089612258E-4	0.0	0.0	0.9536654227396462	0.0
12	1.0763718089612258E-4	0.0	0.0	1.0567818420381316	0.0
13	1.0763718089612258E-4	0.0	0.0	1.1005901746628535	0.0
14	1.0763718089612258E-4	0.0	0.0	1.1156593799883106	0.0
15	1.0763718089612258E-4	0.0	0.0	1.1427839495741334	0.0
16	1.0763718089612258E-4	0.0	0.0	1.2048906029511963	0.0
17	1.0763718089612258E-4	0.0	0.0	1.2824970103773006	0.0
18	1.0763718089612258E-4	0.0	0.0	1.3895960053689427	0.0
19	1.0763718089612258E-4	0.0	0.0	1.4352341700688986	0.0
20	1.0763718089612258E-4	0.0	0.0	1.4850701848238033	0.0
21	1.0763718089612258E-4	0.0	0.0	1.5663362564003758	0.0
22	1.0763718089612258E-4	0.0	0.0	1.6424357432939345	0.0
23	1.0763718089612258E-4	0.0	0.0	1.7428612330700168	0.0
24	1.0763718089612258E-4	0.0	0.0	1.8136864980996656	0.0
25	1.0763718089612258E-4	0.0	0.0	1.87213348732626	0.0
26	1.0763718089612258E-4	0.0	0.0	1.9427434779941166	0.0
27	1.0763718089612258E-4	0.0	0.0	1.9978537146129314	0.0
28	1.0763718089612258E-4	0.0	0.0	2.05285631405085	0.0
29	1.0763718089612258E-4	0.0	0.0	2.125834322698421	0.0
30	1.0763718089612258E-4	0.0	0.0	2.23357914077544	0.0
31	1.0763718089612258E-4	0.0	0.0	2.335296276722276	0.0
32	1.0763718089612258E-4	0.0	0.0	2.4204372868111084	0.0
33	1.0763718089612258E-4	0.0	0.0	2.5037484648247075	0.0
34	1.0763718089612258E-4	0.0	0.0	2.5958858916717884	0.0
35	1.0763718089612258E-4	0.0	0.0	2.743994652584853	0.0
36	1.0763718089612258E-4	0.0	0.0	2.8489409039585727	0.0
37	1.0763718089612258E-4	0.0	0.0	2.95173441171437	0.0
38	1.0763718089612258E-4	0.0	0.0	3.0447329360086197	0.0
39	1.0763718089612258E-4	0.0	0.0	3.1367627256748043	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCACC	25	3.890226E-5	45.0	38
AACGTCC	35	1.2115925E-7	45.0	33
CGTGGCG	25	3.890226E-5	45.0	27
AACCGGG	20	7.032588E-4	45.0	3
CAGCGCG	20	7.032588E-4	45.0	1
AATCCGG	45	3.8380676E-10	45.0	2
TTGGCCA	35	1.2115925E-7	45.0	36
ACACGTT	20	7.032588E-4	45.0	45
ACACGTG	20	7.032588E-4	45.0	16
AGATCGT	40	6.8121153E-9	45.0	19
CGAACAG	20	7.032588E-4	45.0	9
AGTTTCG	25	3.890226E-5	45.0	1
CTCGTAG	20	7.032588E-4	45.0	1
GATCGAG	25	3.890226E-5	45.0	1
TAGACTC	25	3.890226E-5	45.0	5
CCCTATA	35	1.2115925E-7	45.0	32
ACGTTAG	55	1.8189894E-12	45.0	1
GCGAACA	20	7.032588E-4	45.0	8
CATTGCG	20	7.032588E-4	45.0	1
GATCCCC	20	7.032588E-4	45.0	9
>>END_MODULE