##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548918_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3592801
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.966947236988634	31.0	31.0	33.0	30.0	33.0
2	31.29727891970638	31.0	31.0	33.0	30.0	34.0
3	31.457855027317127	31.0	31.0	34.0	30.0	34.0
4	35.26450616107043	37.0	35.0	37.0	33.0	37.0
5	35.1295565771664	37.0	35.0	37.0	32.0	37.0
6	35.19792718828569	37.0	35.0	37.0	32.0	37.0
7	35.63211906253645	36.0	35.0	37.0	35.0	37.0
8	35.72293455718811	37.0	35.0	37.0	35.0	37.0
9	37.575335232872625	39.0	37.0	39.0	35.0	39.0
10	37.14061702832971	39.0	37.0	39.0	33.0	39.0
11	36.59093893594441	39.0	35.0	39.0	32.0	39.0
12	34.588051216863946	35.0	33.0	38.0	30.0	39.0
13	33.92957138455483	35.0	33.0	38.0	25.0	39.0
14	34.41243308493846	35.0	33.0	39.0	25.0	41.0
15	35.04298846498874	35.0	33.0	39.0	30.0	41.0
16	35.378333228030165	35.0	34.0	39.0	31.0	41.0
17	35.28946078560989	35.0	34.0	39.0	31.0	41.0
18	35.44732675146773	36.0	34.0	38.0	31.0	41.0
19	35.29091035100469	36.0	34.0	38.0	31.0	41.0
20	34.93489870438135	35.0	34.0	38.0	30.0	41.0
21	34.739501575511696	35.0	34.0	38.0	29.0	41.0
22	34.56870335985767	35.0	33.0	38.0	29.0	41.0
23	34.588793256292234	35.0	34.0	38.0	29.0	41.0
24	34.710126166186214	35.0	34.0	38.0	30.0	41.0
25	34.6370817643393	35.0	34.0	38.0	30.0	41.0
26	34.391760078000424	35.0	34.0	38.0	29.0	40.0
27	34.37851887705442	35.0	34.0	38.0	29.0	40.0
28	34.57372534688117	35.0	34.0	38.0	29.0	40.0
29	34.63019354537031	35.0	34.0	38.0	29.0	40.0
30	34.616038016021484	35.0	34.0	38.0	29.0	40.0
31	34.25460775589853	35.0	34.0	38.0	29.0	40.0
32	34.06602508739003	35.0	34.0	38.0	29.0	41.0
33	33.8283005376585	35.0	33.0	38.0	27.0	41.0
34	33.679792729961946	35.0	33.0	38.0	26.0	41.0
35	33.43514182945284	35.0	33.0	37.0	24.0	41.0
36	33.106535819824146	35.0	33.0	37.0	23.0	40.0
37	33.10669697542391	35.0	32.0	37.0	23.0	40.0
38	33.232675007605486	35.0	33.0	37.0	23.0	40.0
39	33.29249685690914	35.0	33.0	37.0	23.0	40.0
40	32.862841832876356	35.0	32.0	37.0	23.0	40.0
41	33.075685516676266	35.0	32.0	37.0	23.0	40.0
42	33.08585390618629	35.0	32.0	37.0	23.0	40.0
43	33.06427993089514	35.0	33.0	37.0	23.0	40.0
44	33.03916916077456	35.0	33.0	37.0	23.0	40.0
45	33.115978869968025	35.0	33.0	37.0	23.0	40.0
46	33.164904485386195	35.0	33.0	37.0	23.0	40.0
47	33.13148487767622	35.0	33.0	37.0	23.0	40.0
48	33.18100084029146	35.0	33.0	37.0	23.0	40.0
49	33.26815595965376	35.0	33.0	36.0	24.0	40.0
50	32.97439824805215	35.0	32.0	36.0	24.0	40.0
51	32.68336654326249	35.0	32.0	36.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	9.0
12	7.0
13	11.0
14	22.0
15	79.0
16	281.0
17	809.0
18	2053.0
19	4277.0
20	7873.0
21	12895.0
22	19537.0
23	28334.0
24	40012.0
25	55080.0
26	69541.0
27	81269.0
28	90340.0
29	103080.0
30	124364.0
31	155101.0
32	202546.0
33	276307.0
34	551798.0
35	837423.0
36	145704.0
37	171495.0
38	245482.0
39	366829.0
40	239.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.153103664800806	25.6319233934749	31.63412056498537	13.580852376738928
2	28.60865380520658	27.89258297356297	32.27426178071093	11.224501440519528
3	27.291770404205522	26.62170824379085	34.165348985373804	11.921172366629824
4	26.556438834213196	28.137712052518353	32.41434746872983	12.89150164453862
5	22.346909834416103	32.60798468938302	31.84089516786485	13.204210308336032
6	20.937341088471083	40.73003208360274	29.06039048641993	9.272236341506252
7	87.80962263147889	4.967155152762428	6.1818620068297685	1.041360208928911
8	90.91447035335382	2.7269253153737156	5.591208641948162	0.7673956893242904
9	87.63468948043601	4.343853166373534	6.640946715390025	1.3805106378004237
10	73.56015543304514	12.364113681776418	9.282618213477452	4.793112671700993
11	72.17096076292565	11.234604978121526	12.230735852055263	4.363698406897571
12	61.155933768666834	20.373268655848182	13.494819223218876	4.975978352266101
13	15.868566057513345	63.47894024745595	14.937175757855778	5.715317937174922
14	7.213842347516604	62.40468091608747	24.42712524295111	5.954351493444808
15	6.703738949081789	20.18594962537586	67.53583067918318	5.5744807463591775
16	6.739449248650287	12.50353136730924	65.37414680078301	15.382872583257464
17	7.177213544529741	13.02551964330894	29.77131213223332	50.025954679928
18	14.870653843616722	20.04970495165193	49.125320328067154	15.954320876664196
19	38.58939028351418	14.137465448267244	28.581711038267915	18.691433229950672
20	36.83738676314107	18.981902977648915	28.280525417355427	15.900184841854587
21	15.380729408614616	36.02762858282438	32.390744714221576	16.20089729433943
22	18.78400724114695	23.688787661771414	18.72243967867967	38.80476541840196
23	11.137605450454952	35.24069382078217	23.763409106154224	29.858291622608657
24	8.406338118921699	18.308528638240748	62.38940035921834	10.895732883619216
25	6.603594243043241	22.60024977726292	55.65473846171831	15.14141751797553
26	6.434840114996628	46.83376563299777	29.3068834037844	17.424510848221207
27	7.339204147404768	59.08735273676443	25.139800395290475	8.433642720540325
28	5.777275167759083	37.75449850965862	46.66264566281294	9.805580659769356
29	6.281951045994476	25.81389840405856	44.953644802481406	22.95050574746556
30	12.469212739586746	48.36396449455453	28.18736690398383	10.9794558618749
31	38.77676498086034	30.062756050223765	23.947777792313016	7.212701176602879
32	42.377966383331554	19.95465376456976	30.63203333555073	7.0353465165479525
33	42.27347966113347	26.837640047417043	17.61934490666196	13.269535384787524
34	20.414712643422224	26.55791400636996	21.459524198529227	31.567849151678594
35	18.822222550038255	22.477086818891443	37.181268876289	21.5194217547813
36	46.186220723051456	23.85063909746184	20.43820406418279	9.524936115303909
37	15.214842124570774	49.27356121310365	26.37126297838372	9.140333683941861
38	15.617174455250932	48.072548410001	18.7303722082019	17.57990492654617
39	21.099888360084513	42.74550691786158	24.575143460492246	11.579461261561661
40	31.53692620326035	21.399598808840235	22.13529221351252	24.928182774386894
41	11.515973191946896	22.200199788410213	27.02248190200348	39.261345117639415
42	15.556052227774375	17.72090911798343	25.809472887588264	40.91356576665393
43	16.801181028395394	17.007955631274875	29.91376366239043	36.2770996779393
44	9.238669216580602	32.33864052030714	37.33499294839876	21.087697314713505
45	9.270510668417204	60.80250478665531	18.75121945245506	11.17576509247242
46	19.54107672537388	45.74853992748276	20.658032548977804	14.052350798165552
47	18.27312450647837	29.550286809650743	22.333048782829888	29.843539901040998
48	19.034035005000277	26.408420616672064	42.248513068216134	12.309031310111527
49	25.2192926911343	24.836054098181336	39.48648978888617	10.4581634217982
50	20.097550629717595	49.59548274452161	19.6655478552806	10.64141877048019
51	16.682610587115736	43.33994563016432	21.225250159972678	18.752193622747264
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5322.0
1	6421.0
2	7520.0
3	14530.0
4	21540.0
5	17369.0
6	13198.0
7	14846.0
8	16494.0
9	17966.5
10	19439.0
11	19133.5
12	18828.0
13	18875.5
14	18923.0
15	17082.5
16	15242.0
17	14118.0
18	12994.0
19	11920.0
20	10846.0
21	11571.0
22	12296.0
23	14861.0
24	17426.0
25	19013.5
26	26983.5
27	33366.0
28	47322.5
29	61279.0
30	67909.0
31	74539.0
32	84971.0
33	95403.0
34	102528.5
35	109654.0
36	110719.5
37	111785.0
38	149049.5
39	186314.0
40	316978.0
41	447642.0
42	551041.5
43	654441.0
44	625936.0
45	597431.0
46	501499.0
47	405567.0
48	328407.0
49	251247.0
50	212369.0
51	173491.0
52	125919.5
53	78348.0
54	57364.0
55	36380.0
56	27163.5
57	17947.0
58	15880.5
59	13814.0
60	11715.5
61	9617.0
62	8581.0
63	7545.0
64	6254.5
65	4964.0
66	4463.5
67	3963.0
68	3240.0
69	2517.0
70	2188.0
71	1859.0
72	1484.0
73	1109.0
74	906.0
75	576.0
76	449.0
77	449.0
78	449.0
79	321.0
80	193.0
81	123.0
82	53.0
83	41.5
84	30.0
85	24.0
86	18.0
87	14.0
88	10.0
89	6.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3592801.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.282047456559194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.77352800453671	9.112907761808962
2	8.515999586572672	1.921558229515027
3	2.903291002300915	0.9826520060448071
4	1.33957058659658	0.6045239571757381
5	0.7755019096839818	0.4374624673853482
6	0.5225534625213097	0.3537283777652846
7	0.37884387035045036	0.2991894166744238
8	0.29724413215129014	0.2682817924091687
9	0.23894259734224854	0.2426185550347887
>10	2.5183473601136863	6.228342903323234
>50	0.46004566813613834	3.615378232167098
>100	0.705413184799782	21.128364291573124
>500	0.4796017335314691	38.739035838561975
>1k	0.09012650026094722	14.956179098959996
>5k	4.952005508843255E-4	0.4836279309461536
>10k+	4.952005508843255E-4	0.6261491406548855
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	11360	0.3161878434124239	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11055	0.3076986451517911	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	8753	0.24362607336170303	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	8560	0.23825422003612226	No Hit
ATTGATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	3970	0.11049874457282771	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3917	0.109023572416062	No Hit
GGATATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA	3881	0.10802156868693813	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3790	0.10548872592720832	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009296367931315984	0.0
2	0.0	0.0	0.0	0.04108215289407902	0.0
3	0.0	0.0	0.0	0.06134489497191745	0.0
4	0.0	0.0	0.0	0.09596969050053147	0.0
5	0.0	0.0	0.0	0.17164880548630443	0.0
6	0.0	0.0	0.0	0.24229006838953784	0.0
7	0.0	0.0	0.0	0.2948117638577812	0.0
8	0.0	0.0	0.0	0.4032508340985209	0.0
9	0.0	0.0	0.0	0.44310831576811516	0.0
10	0.0	0.0	0.0	0.5093240622010515	0.0
11	0.0	0.0	0.0	0.606073088935346	0.0
12	0.0	0.0	0.0	0.6789966936660282	0.0
13	0.0	0.0	0.0	0.7116731486102348	0.0
14	0.0	0.0	0.0	0.7224446886983165	0.0
15	0.0	0.0	0.0	0.7417610939208712	0.0
16	0.0	0.0	0.0	0.7940879553306738	0.0
17	0.0	0.0	0.0	0.8517031697552968	0.0
18	0.0	0.0	0.0	0.9436091784654925	0.0
19	0.0	0.0	0.0	0.9787071424217484	0.0
20	0.0	0.0	0.0	1.0175626203622188	0.0
21	0.0	0.0	0.0	1.0804383543647422	0.0
22	0.0	0.0	0.0	1.1340177204359496	0.0
23	0.0	0.0	0.0	1.2153748565534244	0.0
24	0.0	0.0	0.0	1.2673120498463455	0.0
25	0.0	0.0	0.0	1.3101755427033115	0.0
26	0.0	0.0	0.0	1.3598582276057036	0.0
27	0.0	0.0	0.0	1.4011352145582234	0.0
28	0.0	0.0	0.0	1.4463088826795585	0.0
29	0.0	0.0	0.0	1.4982182425355592	2.783343692010774E-5
30	0.0	0.0	0.0	1.5868955725630225	2.783343692010774E-5
31	0.0	0.0	0.0	1.6769924078734113	2.783343692010774E-5
32	0.0	0.0	0.0	1.7434586552386286	2.783343692010774E-5
33	0.0	0.0	0.0	1.8054437192597086	2.783343692010774E-5
34	0.0	0.0	0.0	1.8797868292733162	2.783343692010774E-5
35	0.0	0.0	0.0	2.012023488080748	2.783343692010774E-5
36	0.0	0.0	0.0	2.0953011313457104	2.783343692010774E-5
37	0.0	0.0	0.0	2.1816126192349645	2.783343692010774E-5
38	0.0	0.0	0.0	2.252476549633559	2.783343692010774E-5
39	0.0	0.0	0.0	2.3282669983670123	2.783343692010774E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	45	3.8562575E-10	45.000004	25
TCGCCTC	20	7.0352905E-4	45.000004	19
GTGCACC	25	3.892467E-5	45.000004	18
CAGGTAC	25	3.892467E-5	45.000004	10
ACACGGC	25	3.892467E-5	45.000004	15
ATTAGCC	50	2.1827873E-11	45.000004	29
CACGGGT	90	0.0	45.000004	4
CGTGCGC	25	3.892467E-5	45.000004	18
GACACGT	20	7.0352905E-4	45.000004	8
ATAATCG	160	0.0	45.000004	1
GGCGTGT	80	0.0	45.000004	7
GACGTAA	80	0.0	45.000004	11
CGCATCA	20	7.0352905E-4	45.000004	33
CGCATAT	85	0.0	45.000004	35
CCGGATG	40	6.8212103E-9	45.000004	1
ATAGCCG	45	3.8562575E-10	45.000004	2
CGACACT	20	7.0352905E-4	45.000004	8
TCCAGCG	35	1.2129021E-7	45.000004	6
CGCTCAC	45	3.8562575E-10	45.000004	5
GTGCTAT	25	3.892467E-5	45.000004	23
>>END_MODULE