##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548917_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286937
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.889491421461855	31.0	31.0	33.0	30.0	33.0
2	31.19552724117141	31.0	31.0	33.0	30.0	34.0
3	31.326454936100955	31.0	31.0	34.0	28.0	34.0
4	35.192247775644134	37.0	35.0	37.0	33.0	37.0
5	35.07152789636749	37.0	35.0	37.0	32.0	37.0
6	35.14219846168323	37.0	35.0	37.0	32.0	37.0
7	35.54345727459338	36.0	35.0	37.0	35.0	37.0
8	35.606603540149926	37.0	35.0	37.0	35.0	37.0
9	37.42167444421598	39.0	37.0	39.0	35.0	39.0
10	36.98138267285153	39.0	37.0	39.0	33.0	39.0
11	36.527715143045334	39.0	35.0	39.0	32.0	39.0
12	35.188724354126514	35.0	35.0	39.0	31.0	39.0
13	34.702060034084134	35.0	33.0	39.0	29.0	39.0
14	35.3960799757438	36.0	33.0	40.0	28.0	41.0
15	35.849078369119354	36.0	34.0	40.0	30.0	41.0
16	36.00264169486682	36.0	34.0	40.0	31.0	41.0
17	35.83031815346226	36.0	34.0	40.0	31.0	41.0
18	35.938791442023856	36.0	35.0	40.0	31.0	41.0
19	35.81319592802601	36.0	35.0	40.0	31.0	41.0
20	35.51333567995762	35.0	34.0	40.0	30.0	41.0
21	35.38661448331864	35.0	34.0	40.0	30.0	41.0
22	35.213837880789164	35.0	34.0	40.0	30.0	41.0
23	35.138089545788795	35.0	34.0	39.0	29.0	41.0
24	35.219271129202575	35.0	34.0	39.0	30.0	41.0
25	35.1307882915065	35.0	34.0	39.0	30.0	41.0
26	34.85257739503793	35.0	34.0	39.0	29.0	41.0
27	34.84388210652513	35.0	34.0	39.0	29.0	41.0
28	34.94454880339587	36.0	34.0	39.0	29.0	41.0
29	34.93794108114325	36.0	34.0	39.0	29.0	41.0
30	34.89630824884905	36.0	34.0	39.0	29.0	41.0
31	34.630988683927136	35.0	34.0	39.0	29.0	41.0
32	34.49856588728536	35.0	34.0	39.0	27.0	41.0
33	34.26673102458031	35.0	33.0	39.0	27.0	41.0
34	34.06364811787954	35.0	33.0	39.0	25.0	41.0
35	33.79876767374023	35.0	33.0	39.0	23.0	41.0
36	33.56185504135054	35.0	33.0	39.0	23.0	41.0
37	33.60779892450259	35.0	33.0	39.0	23.0	41.0
38	33.63492334554275	35.0	33.0	39.0	23.0	41.0
39	33.64124180569254	35.0	33.0	39.0	23.0	41.0
40	33.3338224070092	35.0	32.0	39.0	23.0	41.0
41	33.42979120852313	35.0	33.0	39.0	23.0	41.0
42	33.36547743929852	35.0	33.0	39.0	23.0	41.0
43	33.338067241241106	35.0	33.0	39.0	23.0	41.0
44	33.2869758866929	35.0	33.0	39.0	23.0	41.0
45	33.33762463537292	35.0	33.0	39.0	23.0	41.0
46	33.36964560164775	35.0	33.0	39.0	23.0	41.0
47	33.3073218162872	35.0	33.0	39.0	23.0	41.0
48	33.336864886717294	35.0	33.0	39.0	23.0	41.0
49	33.37268808135584	35.0	33.0	39.0	23.0	41.0
50	33.16509198883379	35.0	32.0	39.0	23.0	41.0
51	32.89655220483939	35.0	32.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	1.0
15	10.0
16	29.0
17	96.0
18	211.0
19	386.0
20	703.0
21	1067.0
22	1593.0
23	2313.0
24	3249.0
25	4584.0
26	5758.0
27	6622.0
28	7294.0
29	8083.0
30	9498.0
31	11699.0
32	14745.0
33	19477.0
34	35908.0
35	51904.0
36	16252.0
37	19336.0
38	28202.0
39	37903.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.90269292562479	25.192289596671046	27.67192798419165	14.233089493512512
2	31.081038694905157	26.756047494746234	29.93305150607973	12.229862304268883
3	28.3271240725316	26.490483973833978	33.492020896573116	11.690371057061306
4	26.647312824766413	27.87998759309535	32.19696309642883	13.275736485709407
5	22.579520940136685	32.866448035631514	32.267361825069614	12.286669199162185
6	21.162136636265103	41.76700808888362	28.823051750035724	8.247803524815552
7	85.49507383153794	5.414080442745271	7.692977901072361	1.3978678246444343
8	86.83195265859753	4.363675650055587	7.615260492721399	1.189111198625482
9	81.92983128700725	6.991778683125564	9.001279026406493	2.07711100346069
10	58.85368565225119	21.323844607004325	13.040144700753126	6.782325039991358
11	56.194913866109985	17.412533064749404	19.373242209962466	7.019310859178147
12	49.93291210265668	22.471483287272118	19.62312284578148	7.972481764289722
13	16.96016895694874	53.30682344904979	20.100231061173705	9.632776532827764
14	9.657869148976953	52.37944217720266	27.23698930427237	10.72569936954802
15	10.505093452569728	22.908164509979542	57.11358242401642	9.473159613434309
16	10.350007144425431	18.756382062961556	55.14241802207454	15.75119277053848
17	10.577583232556275	18.55982323645957	31.352875369854704	39.50971816112945
18	16.029999616640588	24.189281967818722	44.34806246667387	15.432655948866824
19	35.12199542059755	18.983958151092402	28.557836737681093	17.336209690628955
20	33.18498485730317	22.18919135559374	29.353481774744978	15.272342012358115
21	16.98770113300132	36.147656105695674	30.971607007810075	15.893035753492926
22	19.149499715965526	25.808801235114327	22.071743971673225	32.96995507724692
23	14.197541620634494	35.00350251100416	26.72712128446314	24.07183458389821
24	11.06410117900445	21.935825634198448	55.35222017376636	11.647853013030735
25	10.205027584452337	27.32585898646741	47.38949664909022	15.079616779990031
26	9.78925687520257	44.249086036307624	29.171211799105727	16.79044528938408
27	10.614176631107176	50.533392347449094	27.791814928015558	11.060616093428175
28	9.07098073793202	35.41439410044714	44.0281316107717	11.486493550849142
29	10.087231691974196	27.59874118708985	41.69939742870386	20.614629692232093
30	14.65617888247246	44.355729654941676	28.862433217047645	12.125658245538219
31	33.95414324398735	29.41760734934846	25.892094780387332	10.736154626276848
32	36.172051704729604	22.903982407288012	30.01983013692901	10.904135751053367
33	35.45482109313195	27.54855595479147	21.74414592750325	15.252477024573338
34	19.424472967933728	27.4203048055845	24.89884539114858	28.256376835333192
35	19.8479108654513	24.33391301923419	36.563775323503066	19.25440079181144
36	37.82223972509645	27.945507201929342	23.231928960015612	11.000324112958594
37	15.041280838650994	46.31365073169372	27.78519326542063	10.859875164234657
38	16.270470521403656	44.90707019310859	22.6844220159826	16.138037269505155
39	20.757518200859423	40.4865876481597	25.666609743602255	13.089284407378624
40	28.257422361006075	25.36340729846621	24.800914486455213	21.5782558540725
41	12.809083527046006	25.994556296329858	27.408107006067535	33.7882531705566
42	15.730282257080821	22.64050993772152	26.416948668174545	35.21225913702311
43	17.372454580622225	22.57708138023329	29.09558544210053	30.95487859704395
44	11.722433844363048	32.814520260545	35.41927322025392	20.04377267483803
45	11.859746216068336	54.01429582103388	20.717788225289873	13.408169737607906
46	17.702840693253222	43.82704217302056	23.24273272530207	15.22738440842415
47	17.436231646668084	30.21046431795133	25.915444853748383	26.437859181632206
48	17.59131795481238	28.24592157860436	40.42943224470877	13.733328221874489
49	21.944886856696765	28.832809989649295	37.76508432164552	11.45721883200842
50	17.705977270271873	45.78112965563869	24.094139131586374	12.418753942503058
51	16.39907017916825	39.935944127108044	24.969941136904616	18.69504455681909
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	398.0
1	612.5
2	827.0
3	1947.5
4	3068.0
5	2385.5
6	1703.0
7	1791.0
8	1879.0
9	1957.0
10	2035.0
11	1980.0
12	1925.0
13	1796.5
14	1668.0
15	1617.5
16	1567.0
17	1461.0
18	1355.0
19	1449.5
20	1544.0
21	1678.0
22	1812.0
23	1652.5
24	1493.0
25	2347.5
26	3757.0
27	4312.0
28	5770.0
29	7228.0
30	8267.0
31	9306.0
32	9669.5
33	10033.0
34	10764.0
35	11495.0
36	11558.0
37	11621.0
38	14465.0
39	17309.0
40	24886.0
41	32463.0
42	37465.5
43	42468.0
44	40804.0
45	39140.0
46	32580.5
47	26021.0
48	21252.5
49	16484.0
50	14026.5
51	11569.0
52	8791.0
53	6013.0
54	4759.5
55	3506.0
56	3164.5
57	2823.0
58	2594.5
59	2366.0
60	2360.5
61	2355.0
62	2144.5
63	1934.0
64	1558.5
65	1183.0
66	997.5
67	812.0
68	727.0
69	642.0
70	584.5
71	527.0
72	442.5
73	358.0
74	292.0
75	171.0
76	116.0
77	92.0
78	68.0
79	56.0
80	44.0
81	30.5
82	17.0
83	14.0
84	11.0
85	9.0
86	7.0
87	4.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	286937.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.186713459749004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.0611595406116	18.653572038461405
2	7.563304275633042	3.809895551985279
3	3.6751072367510726	2.776916187176976
4	2.2056178220561784	2.222090563433785
5	1.448733914487339	1.8244422991806564
6	0.964438909644389	1.4574627879987594
7	0.741663207416632	1.3076041082188774
8	0.582537705825377	1.1737768220898666
9	0.4593884045938841	1.041343570191366
>10	7.30178497301785	46.08049850664083
>50	0.8744984087449842	14.03060602153086
>100	0.11484710114847102	4.089050906645013
>500	0.004151100041511001	0.6353311005551742
>1k	0.0027674000276740004	0.8974095358911537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1527	0.5321725674973949	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1048	0.3652369683937589	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	692	0.2411679218783217	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	618	0.2153782886138769	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	513	0.1787848900629755	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	500	0.17425427881381628	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	399	0.13905491449342539	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	312	0.10873466997982137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01115227384408424	0.0
2	0.0	0.0	0.0	0.038684449896667215	0.0
3	0.0	0.0	0.0	0.05297330075940015	0.0
4	0.0	0.0	0.0	0.08224801960012128	0.0
5	0.0	0.0	0.0	0.15613183381717938	0.0
6	0.0	0.0	0.0	0.2237424939969401	0.0
7	0.0	0.0	0.0	0.2767157947563402	0.0
8	0.0	0.0	0.0	0.3976482642531287	0.0
9	0.0	0.0	0.0	0.4419088510718381	0.0
10	0.0	0.0	0.0	0.5022008315414185	0.0
11	0.0	0.0	0.0	0.568068948933041	0.0
12	0.0	0.0	0.0	0.6203452325771859	0.0
13	0.0	0.0	0.0	0.6506654770907899	0.0
14	0.0	0.0	0.0	0.6597266995891085	0.0
15	0.0	0.0	0.0	0.67715212747049	0.0
16	0.0	0.0	0.0	0.7133970174637638	0.0
17	0.0	0.0	0.0	0.7485963817841547	0.0
18	0.0	0.0	0.0	0.8127219563876391	0.0
19	0.0	0.0	0.0	0.8381630810944563	0.0
20	0.0	0.0	0.0	0.8660437657046669	0.0
21	0.0	0.0	0.0	0.908561809735238	0.0
22	0.0	0.0	0.0	0.9559589735725961	0.0
23	0.0	0.0	0.0	1.0127658684659002	0.0
24	0.0	0.0	0.0	1.0549354039388437	0.0
25	0.0	0.0	0.0	1.0894377511439794	0.0
26	0.0	0.0	0.0	1.131607286616923	0.0
27	0.0	0.0	0.0	1.1657611252644309	0.0
28	0.0	0.0	0.0	1.2058396093916086	0.0
29	0.0	0.0	0.0	1.251145721883201	0.0
30	0.0	0.0	0.0	1.3229384847544932	0.0
31	0.0	0.0	0.0	1.398913350317317	0.0
32	0.0	0.0	0.0	1.4665240104970778	0.0
33	0.0	0.0	0.0	1.5212398540446161	0.0
34	0.0	0.0	0.0	1.580137800283686	0.0
35	0.0	0.0	0.0	1.6850388761296033	0.0
36	0.0	0.0	0.0	1.761362250250055	0.0
37	0.0	0.0	0.0	1.828624401872188	0.0
38	0.0	0.0	0.0	1.9045992674350118	0.0
39	0.0	0.0	0.0	1.9823166757859738	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGAGG	35	1.2076634E-7	45.000004	2
GCGTTAG	25	3.883464E-5	45.000004	1
AGTATCG	25	3.883464E-5	45.000004	1
TGTTACG	25	3.883464E-5	45.000004	1
GCGATTC	35	1.2076634E-7	45.000004	9
TATCGGA	25	3.883464E-5	45.000004	35
GTACGAG	35	1.2076634E-7	45.000004	1
AGGTCAA	20	7.024429E-4	45.0	33
GCCCCGT	20	7.024429E-4	45.0	6
AGCGTGG	20	7.024429E-4	45.0	2
AAATCGA	20	7.024429E-4	45.0	44
GTCTGAT	20	7.024429E-4	45.0	33
GCGAAAG	20	7.024429E-4	45.0	1
AGCCCCG	20	7.024429E-4	45.0	5
GGCACCG	45	3.8198777E-10	45.0	8
GTTTACG	20	7.024429E-4	45.0	1
CAGCACA	95	0.0	45.0	45
CGGCAGG	20	7.024429E-4	45.0	2
ATAGCGG	45	3.8198777E-10	45.0	2
ATTGTGT	20	7.024429E-4	45.0	21
>>END_MODULE