##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548916_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4361558
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.911038211574855	31.0	31.0	33.0	28.0	34.0
2	31.198001493961563	31.0	31.0	34.0	28.0	34.0
3	31.278715083004744	31.0	31.0	34.0	28.0	34.0
4	35.09933629221484	35.0	35.0	37.0	32.0	37.0
5	34.958865845645064	35.0	35.0	37.0	32.0	37.0
6	35.019313969916254	37.0	35.0	37.0	32.0	37.0
7	35.59690757293609	37.0	35.0	37.0	35.0	37.0
8	35.5385293512089	37.0	35.0	37.0	35.0	37.0
9	37.24695831168587	39.0	37.0	39.0	34.0	39.0
10	36.54854733102254	39.0	35.0	39.0	32.0	39.0
11	36.20184369897179	38.0	35.0	39.0	31.0	39.0
12	35.5931600588597	37.0	35.0	39.0	30.0	39.0
13	35.18015282612314	37.0	35.0	39.0	30.0	39.0
14	36.18488095309061	38.0	35.0	40.0	30.0	41.0
15	36.49928030304767	38.0	35.0	40.0	31.0	41.0
16	36.569711327924566	38.0	35.0	40.0	31.0	41.0
17	36.47426194951437	38.0	35.0	40.0	31.0	41.0
18	36.413469682164035	38.0	35.0	40.0	31.0	41.0
19	36.34051616417803	38.0	35.0	40.0	30.0	41.0
20	36.21279918781316	38.0	34.0	40.0	30.0	41.0
21	36.0583229662428	37.0	34.0	40.0	30.0	41.0
22	35.95296245057386	37.0	34.0	40.0	30.0	41.0
23	35.920813617519244	37.0	34.0	40.0	30.0	41.0
24	35.82879466465882	37.0	34.0	40.0	30.0	41.0
25	35.7249989567948	37.0	34.0	40.0	30.0	41.0
26	35.58934422057439	37.0	34.0	40.0	29.0	41.0
27	35.48705623082394	37.0	34.0	40.0	29.0	41.0
28	35.51665826752734	37.0	34.0	40.0	29.0	41.0
29	35.543254497590084	37.0	34.0	40.0	29.0	41.0
30	35.41037812634843	37.0	34.0	40.0	29.0	41.0
31	35.25472571957085	37.0	34.0	40.0	28.0	41.0
32	35.03363110154674	37.0	34.0	40.0	27.0	41.0
33	34.8179212565785	37.0	33.0	40.0	25.0	41.0
34	34.64811633824427	37.0	34.0	40.0	24.0	41.0
35	34.48064453115148	37.0	33.0	40.0	24.0	41.0
36	34.3252328640362	37.0	33.0	40.0	23.0	41.0
37	34.29525550273549	37.0	33.0	40.0	23.0	41.0
38	34.27122097195544	37.0	33.0	40.0	23.0	41.0
39	34.219070112102145	36.0	33.0	40.0	23.0	41.0
40	34.08336699867341	36.0	33.0	40.0	23.0	41.0
41	34.02864251719225	36.0	33.0	40.0	23.0	41.0
42	34.008367652109634	36.0	33.0	40.0	23.0	41.0
43	33.91881112208069	36.0	33.0	40.0	23.0	41.0
44	33.848225106716455	36.0	33.0	40.0	23.0	41.0
45	33.80205307369523	36.0	33.0	40.0	23.0	41.0
46	33.74259106493597	35.0	33.0	40.0	23.0	41.0
47	33.66422801210026	35.0	32.0	40.0	23.0	41.0
48	33.63094586842592	35.0	32.0	40.0	23.0	41.0
49	33.67073325632721	36.0	32.0	40.0	23.0	41.0
50	33.595530312791894	35.0	32.0	40.0	23.0	41.0
51	33.392479705646466	35.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	6.0
13	19.0
14	58.0
15	164.0
16	575.0
17	1593.0
18	3607.0
19	6653.0
20	11129.0
21	17063.0
22	24948.0
23	34971.0
24	49513.0
25	68357.0
26	86620.0
27	99178.0
28	106387.0
29	119018.0
30	140385.0
31	167994.0
32	205781.0
33	258785.0
34	396689.0
35	512573.0
36	328911.0
37	399028.0
38	548812.0
39	772483.0
40	252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.384574502964306	23.736105309157875	28.032161901779133	15.847158286098683
2	33.47987118364584	24.992972694619674	28.840198846375536	12.686957275358942
3	29.864488790473498	25.329389176986755	31.948170814190707	12.85795121834904
4	25.9435504468816	27.613251961798973	31.8320196590301	14.611177932289332
5	22.68797067469927	31.962271280125133	31.27781402884015	14.071944016335447
6	20.8643562690213	40.790997162023295	28.53102033722812	9.813626231727286
7	86.1632700975202	4.048782568063981	8.35192378503278	1.4360235493830416
8	87.3017852794804	3.377233548195393	7.932715786423109	1.3882653859010932
9	82.72938248213138	5.471347623945388	9.347003983438945	2.452265910484281
10	44.208514480376046	30.282481626978246	15.408943317961151	10.100060574684552
11	37.312102693578765	22.832162268620525	26.331485216979804	13.524249820820907
12	35.28406592323202	22.986212724902433	27.667085935805506	14.06263541606004
13	22.453673664319034	35.04713224035998	27.13152043375326	15.367673661567723
14	16.801679583304864	36.94120312053629	30.387054350761815	15.870062945397034
15	16.302477234052603	24.560581333550992	43.69427163412707	15.442669798269334
16	18.38709928883211	21.106288165834318	42.20372628313094	18.302886262202637
17	17.766862208412682	21.46345411433254	31.754065863620294	29.015617813634485
18	21.200589330693298	24.060622373931515	35.30621397216316	19.432574323212027
19	26.859392905012385	25.058385100003257	28.748901195398524	19.333320799585834
20	28.168489333398757	24.42799109859367	29.07701789131315	18.326501676694427
21	23.097549086817143	26.832705193877963	31.129862310669722	18.939883408635172
22	22.270184186476484	24.91343689571479	28.837608029057506	23.97877088875122
23	19.09166403381544	28.80736654195588	29.518855418178553	22.582114006050134
24	19.01137162454334	24.323349592049446	38.774813954096224	17.890464829310996
25	18.78055960736966	26.27152957727491	35.03213759853704	19.915773216818394
26	17.762368401383174	31.980934335849714	29.882372308243983	20.374324954523132
27	17.77722547768481	32.06473466591525	31.26992693895163	18.888112917448307
28	16.490047822360726	29.190211387765565	37.08894849042475	17.230792299448957
29	17.733640135015975	25.77948063513084	36.5052350559135	19.98164417393968
30	19.105420585946582	29.417790615188427	32.95285767150179	18.523931127363202
31	25.957559202468477	27.218599408743387	28.202307524054476	18.621533864733657
32	26.749500981071446	26.75046393972062	29.710965668689948	16.789069410517985
33	25.6764211320817	27.988484848762756	27.43877302560232	18.89632099355322
34	19.85322675979547	28.48823746010027	30.06453198604719	21.59400379405708
35	20.237126274601874	26.459604572494506	31.82807611408584	21.475193038817782
36	26.46811070722893	27.834365609720198	27.909224180900495	17.78829950215038
37	20.364053395598543	31.94489216926612	29.498541576198228	18.192512858937103
38	20.074844814628168	32.5884924607216	26.70444827284195	20.632214451808277
39	20.92332602249013	31.462679161895817	27.80985601933988	19.804138796274177
40	22.79050284325005	26.82037015213371	28.009394808002096	22.379732196614146
41	18.106878321920743	26.51591472588465	29.556089819280174	25.821117132914427
42	20.70434464014923	27.35942064739251	27.2297651435565	24.706469568901753
43	21.111676148752352	27.438452039385925	28.082121113602064	23.36775069825966
44	19.72767529401191	29.98249708017181	29.507276986801507	20.782550639014772
45	18.280829923619038	34.69168586087815	26.17009793289462	20.857386282608186
46	20.964068344385193	32.39707003781676	27.332847574192527	19.30601404360552
47	20.979567393119613	29.440030374467106	27.642324141969453	21.938078090443828
48	20.987041786444202	27.338762891608916	31.01985574879435	20.65433957315253
49	20.614491427145985	26.63633041220591	31.88658731581696	20.862590844831136
50	19.477328972812007	31.943814572682516	28.583799642237935	19.995056812267542
51	18.52361014114681	32.19202862830209	27.455601874376086	21.828759356175016
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3126.0
1	5292.5
2	7459.0
3	27556.0
4	47653.0
5	36230.5
6	24808.0
7	25364.5
8	25921.0
9	26785.5
10	27650.0
11	27397.5
12	27145.0
13	25876.5
14	24608.0
15	23531.0
16	22454.0
17	21252.5
18	20051.0
19	20695.0
20	21339.0
21	21229.5
22	21120.0
23	23775.0
24	26430.0
25	31063.0
26	42074.5
27	48453.0
28	61452.0
29	74451.0
30	85301.0
31	96151.0
32	108807.0
33	121463.0
34	134317.0
35	147171.0
36	160129.0
37	173087.0
38	189581.0
39	206075.0
40	252514.5
41	298954.0
42	350088.5
43	401223.0
44	406428.5
45	411634.0
46	386438.0
47	361242.0
48	344391.5
49	327541.0
50	302821.5
51	278102.0
52	260396.0
53	242690.0
54	221103.5
55	199517.0
56	186621.5
57	173726.0
58	161201.0
59	148676.0
60	137572.0
61	126468.0
62	105338.5
63	84209.0
64	65463.0
65	46717.0
66	34907.0
67	23097.0
68	18801.5
69	14506.0
70	11240.5
71	7975.0
72	7186.5
73	6398.0
74	5051.5
75	2604.5
76	1504.0
77	1076.0
78	648.0
79	484.0
80	320.0
81	257.0
82	194.0
83	160.0
84	126.0
85	88.0
86	50.0
87	33.0
88	16.0
89	9.0
90	2.0
91	3.0
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4361558.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.471814499968808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.59828023329054	12.624734552859426
2	6.582679167016947	2.036919819697908
3	2.266968092517901	1.0522232941435528
4	1.2138065641436748	0.7511915999710171
5	0.7585502155837169	0.5868074112211308
6	0.5463705390648723	0.5072006177195798
7	0.4098162706551724	0.44384209230520866
8	0.3373753377535047	0.4175846914069237
9	0.2660676889133635	0.3704894934572671
>10	3.0215610934691006	11.11829134384123
>50	1.203169424447564	13.571537796657173
>100	1.7712681401657115	52.9105201887228
>500	0.020047759124651034	1.997227438190462
>1k	0.00374025356803191	0.8393248601553741
>5k	1.496101427212764E-4	0.22001607269103232
>10k+	1.496101427212764E-4	0.552088726959896
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23851	0.5468458747997849	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9505	0.21792671334417654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0124955348524541	0.0
2	0.0	0.0	0.0	0.042668239193425835	0.0
3	0.0	0.0	0.0	0.06275280530489334	0.0
4	0.0	0.0	0.0	0.08907367504914528	0.0
5	0.0	0.0	0.0	0.14866247336387595	0.0
6	0.0	0.0	0.0	0.2155880994818824	0.0
7	2.292758688523688E-5	0.0	0.0	0.25995298010481577	0.0
8	4.585517377047376E-5	0.0	0.0	0.3714956903014932	0.0
9	4.585517377047376E-5	0.0	0.0	0.4192309261965564	0.0
10	4.585517377047376E-5	0.0	0.0	0.49998188720636066	0.0
11	4.585517377047376E-5	0.0	0.0	0.5812831103014107	0.0
12	4.585517377047376E-5	0.0	0.0	0.659328616058757	0.0
13	4.585517377047376E-5	0.0	0.0	0.6908311204390725	0.0
14	4.585517377047376E-5	0.0	0.0	0.7037164242685756	0.0
15	4.585517377047376E-5	0.0	0.0	0.7256122697439769	0.0
16	4.585517377047376E-5	0.0	0.0	0.77286602631445	0.0
17	6.878276065571064E-5	0.0	0.0	0.8294971659209851	0.0
18	6.878276065571064E-5	0.0	0.0	0.9190981754684908	0.0
19	6.878276065571064E-5	0.0	0.0	0.9559428075930665	0.0
20	6.878276065571064E-5	0.0	0.0	0.9984964088520661	0.0
21	9.171034754094752E-5	0.0	0.0	1.0454062516192608	0.0
22	1.146379344261844E-4	0.0	0.0	1.0988046014749775	0.0
23	1.146379344261844E-4	0.0	0.0	1.1606173757175762	0.0
24	1.146379344261844E-4	0.0	0.0	1.208604815068377	0.0
25	1.146379344261844E-4	0.0	0.0	1.249645195592951	0.0
26	1.146379344261844E-4	0.0	0.0	1.2912816933765412	0.0
27	1.146379344261844E-4	0.0	0.0	1.3336289463535738	0.0
28	1.146379344261844E-4	0.0	0.0	1.3807909925765058	0.0
29	1.146379344261844E-4	0.0	0.0	1.431323394071568	0.0
30	1.146379344261844E-4	0.0	0.0	1.5000602995535082	0.0
31	1.3756552131142128E-4	0.0	0.0	1.5644638911141386	0.0
32	1.3756552131142128E-4	0.0	0.0	1.6235712101042792	0.0
33	1.3756552131142128E-4	0.0	0.0	1.6874703947534344	0.0
34	1.3756552131142128E-4	0.0	0.0	1.7522408277042287	0.0
35	1.3756552131142128E-4	0.0	0.0	1.8331064266484591	0.0
36	1.3756552131142128E-4	0.0	0.0	1.902783363192694	0.0
37	1.6049310819665817E-4	0.0	0.0	1.9762662791598782	0.0
38	1.6049310819665817E-4	0.0	0.0	2.0517668227729633	0.0
39	1.8342069508189504E-4	0.0	0.0	2.134122714864734	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGA	20	7.035457E-4	45.000004	13
CGCACGA	25	3.8926046E-5	45.000004	40
CGTTTTT	22155	0.0	43.31415	1
CGCATCG	125	0.0	43.2	21
CGACGGT	610	0.0	41.68033	28
TGCGTAG	425	0.0	41.294117	1
ACGGGAT	1620	0.0	40.694443	5
CGGTCTA	625	0.0	40.32	31
TAATGCG	235	0.0	40.212765	1
GCGATAT	325	0.0	39.46154	9
CGTTAGG	730	0.0	39.452057	2
TCACGAC	645	0.0	39.418602	25
CTATGCG	150	0.0	39.0	1
CGGGATC	870	0.0	38.793106	6
AGGGATC	4520	0.0	38.62832	6
GCGATCG	35	6.252307E-6	38.571426	9
TAACGGG	910	0.0	38.32418	3
TACGGGA	1260	0.0	38.214287	4
GTACGGG	1840	0.0	38.152176	3
TAGGGAC	3180	0.0	38.066036	5
>>END_MODULE