FastQCFastQC Report
Sat 18 Jun 2016
SRR3548913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1709368
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98060.5736623126208049No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC84830.4962652863514469No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51540.30151494587473265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG302.1661253E-645.00000431
TCTGCGA207.0342526E-445.045
CTATGCG502.1827873E-1145.01
GTACCTC207.0342526E-445.041
GATCGAC207.0342526E-445.010
AACCCGC207.0342526E-445.017
AAGCGTC207.0342526E-445.036
TAACGCG502.1827873E-1145.01
GTAATCG253.891607E-545.013
CTACGGC207.0342526E-445.036
ACGTCCA406.8175723E-945.010
GGTTCGC207.0342526E-445.08
TCGACGC207.0342526E-445.042
TATCGTG502.1827873E-1145.01
ATCGATC207.0342526E-445.019
CGTTTTT91150.042.852441
TGCGAAG750.042.01
ACGGGTA2350.041.170215
CGTAAGG2250.041.02
TCGATTG556.184564E-1140.9090921
Produced by FastQC (version 0.11.5)