##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548913_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1709368
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.907897538739466	31.0	31.0	33.0	28.0	34.0
2	31.191245536361976	31.0	31.0	34.0	28.0	34.0
3	31.261064323188453	31.0	31.0	34.0	28.0	34.0
4	35.08594287479349	35.0	35.0	37.0	32.0	37.0
5	34.95053376452584	35.0	35.0	37.0	32.0	37.0
6	35.00979660318902	36.0	35.0	37.0	32.0	37.0
7	35.53714179743625	37.0	35.0	37.0	35.0	37.0
8	35.45973658100538	37.0	35.0	37.0	35.0	37.0
9	37.12602026011953	39.0	37.0	39.0	34.0	39.0
10	36.58957989151546	39.0	35.0	39.0	32.0	39.0
11	36.27493494671715	38.0	35.0	39.0	32.0	39.0
12	35.2953571144423	37.0	35.0	39.0	30.0	39.0
13	34.74174022211718	37.0	33.0	39.0	27.0	39.0
14	35.668500872837214	37.0	34.0	40.0	27.0	41.0
15	36.088211549531756	38.0	34.0	40.0	30.0	41.0
16	36.23795227241881	37.0	34.0	40.0	31.0	41.0
17	36.150245002831454	37.0	34.0	40.0	31.0	41.0
18	36.0711023021374	37.0	35.0	40.0	30.0	41.0
19	35.94109752844326	37.0	34.0	40.0	30.0	41.0
20	35.770017924753475	36.0	34.0	40.0	30.0	41.0
21	35.58252991748997	36.0	34.0	40.0	30.0	41.0
22	35.47062423070983	36.0	34.0	40.0	29.0	41.0
23	35.45561107965049	35.0	34.0	40.0	29.0	41.0
24	35.34570554731339	35.0	34.0	40.0	29.0	41.0
25	35.22733957813648	35.0	34.0	40.0	29.0	41.0
26	35.08468802504785	35.0	34.0	40.0	29.0	41.0
27	34.93749619742501	35.0	34.0	40.0	28.0	41.0
28	34.96776469431977	36.0	34.0	40.0	28.0	41.0
29	35.00735827510518	36.0	34.0	40.0	29.0	41.0
30	34.906669599524506	36.0	34.0	40.0	28.0	41.0
31	34.7084185500138	35.0	33.0	40.0	27.0	41.0
32	34.42445044016268	35.0	33.0	40.0	25.0	41.0
33	34.1719454207637	35.0	33.0	40.0	24.0	41.0
34	34.00157660609067	35.0	33.0	40.0	23.0	41.0
35	33.79218810694947	35.0	33.0	40.0	23.0	41.0
36	33.56246577682512	35.0	33.0	40.0	22.0	41.0
37	33.4950777129325	35.0	33.0	40.0	22.0	41.0
38	33.48593690767582	35.0	32.0	40.0	23.0	41.0
39	33.447625672178255	35.0	33.0	40.0	22.0	41.0
40	33.2490476012187	35.0	32.0	39.0	21.0	41.0
41	33.235790654791714	35.0	32.0	39.0	21.0	41.0
42	33.195139373148436	35.0	32.0	39.0	21.0	41.0
43	33.062755942547184	35.0	32.0	39.0	21.0	41.0
44	32.98475869444145	35.0	32.0	39.0	20.0	41.0
45	32.93883938391265	35.0	32.0	39.0	20.0	41.0
46	32.89035245775047	35.0	32.0	39.0	20.0	41.0
47	32.809545399235276	35.0	32.0	39.0	20.0	41.0
48	32.78273724557848	35.0	32.0	39.0	20.0	41.0
49	32.8137036612362	35.0	32.0	39.0	20.0	41.0
50	32.68590789110361	35.0	32.0	39.0	20.0	40.0
51	32.49889432819615	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	3.0
12	2.0
13	10.0
14	19.0
15	89.0
16	290.0
17	730.0
18	1632.0
19	3123.0
20	5252.0
21	8222.0
22	11877.0
23	16710.0
24	22988.0
25	30554.0
26	37862.0
27	42878.0
28	46283.0
29	51320.0
30	59307.0
31	71451.0
32	87812.0
33	111459.0
34	181963.0
35	242448.0
36	119286.0
37	136523.0
38	179109.0
39	240061.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.650546868784254	23.943586167519225	27.61506006898456	15.790806894711965
2	32.33177408258491	26.57643058721118	28.460401739122293	12.631393591081617
3	29.083556027724867	25.60589644827796	32.699746338997805	12.610801184999367
4	26.130359290685213	27.82039911827061	30.846546793902775	15.2026947971414
5	23.197345451652307	32.122807961773006	30.81027607864427	13.869570507930417
6	20.805642787275765	40.64355949099316	28.292269423553034	10.25852829817804
7	81.92244151054659	6.917117905565099	9.287409147708393	1.8730314361799214
8	82.85483289730473	6.083066958080413	8.847538973468556	2.214561171146295
9	78.0246851467911	7.542846244927951	11.002896977128389	3.4295716311525664
10	49.46272540494498	23.547533357357807	16.42940548787622	10.560335749820988
11	46.400014508286105	21.570369867693792	20.518928633272647	11.510686990747459
12	41.04206934960757	22.445488625035686	24.136171965311156	12.376270060045584
13	21.927987419911922	41.92280421769917	23.305397082430463	12.843811279958443
14	14.631314029512662	44.28788885716826	28.091317960790185	12.989479152528888
15	13.181304435323465	24.178351297087577	49.57563263147549	13.064711636113463
16	14.858591011414745	19.359318765766062	47.003395406957424	18.77869481586177
17	15.259440916174865	19.80310851729996	30.515956774667597	34.42149379185758
18	20.45533787926298	24.36514548066888	35.75888866528448	19.420627974783663
19	29.01680621141849	24.10306031234936	27.551761820743103	19.328371655489047
20	30.355956119454675	23.56888627843741	26.069810596664965	20.005347005442946
21	21.387904769482056	28.82691146669412	29.919128005204264	19.86605575861956
22	21.250368557267947	26.051090227499284	26.378930692513258	26.319610522719504
23	18.342276209686855	30.792316224475947	26.290769453973635	24.574638111863568
24	18.435176041671543	24.549658119258112	40.453781748576084	16.561384090494265
25	17.09421259787243	25.14168979412274	37.39820799266161	20.365889615343214
26	15.378373761530579	35.18042925806497	29.20576493768457	20.235432042719882
27	17.001546770502316	35.71869837273191	29.5097369320123	17.770017924753475
28	14.36513378043815	30.903643919858098	38.94380847190307	15.787413827800684
29	14.982788960598302	26.08952548544257	38.41273499913419	20.51495055482494
30	17.57947966733904	32.19698742459201	32.64586677649283	17.577666131576112
31	27.987478413074307	28.12437111259834	26.353190184910446	17.534960289416908
32	29.53144086001376	27.70620486635996	27.610613981307715	15.151740292318564
33	28.11272938302343	28.503341585896074	24.93939280482611	18.44453622625438
34	19.530610143632032	29.65868086918674	28.16227986015884	22.648429127022386
35	20.546599678945668	27.172498841677157	31.222475207211087	21.058426272166088
36	29.306035915028243	27.245917789498808	26.107660843071823	17.340385452401122
37	20.42491727936875	34.17888950770109	28.337841822240733	17.058351390689424
38	20.476047287652513	34.529779427250304	23.95013829672721	21.04403498836997
39	20.377297340303553	33.01939664250179	27.073514889713625	19.529791127481033
40	22.97761511857014	27.22684641341127	26.162534925188723	23.633003542829865
41	17.27486416032124	25.936603469820422	28.017021495663897	28.77151087419444
42	21.71884579563909	26.72361948977634	24.60026161715909	26.95727309742548
43	22.196098207056643	26.65324260194411	26.63867581468707	24.51198337631218
44	18.629692377533686	31.29829270233209	29.069515750850606	21.002499169283617
45	17.413628896761843	38.23670502782315	23.811666066054823	20.538000009360186
46	21.192978925544413	34.2268019525345	25.985802940033974	18.59441618188711
47	21.956360479428653	29.31422607653823	26.97909402773423	21.75031941629889
48	22.73196877442423	26.619253431677674	30.56369371604008	20.085084077858014
49	20.950784149463427	26.04096952791909	32.09490291148541	20.913343411132068
50	19.074535149833157	34.35369095478563	26.93153258982267	19.640241305558543
51	18.218370766271512	34.22516392023251	24.950098515942734	22.60636679755325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6105.0
1	5603.0
2	5101.0
3	11765.5
4	18430.0
5	14179.0
6	9928.0
7	10763.0
8	11598.0
9	12704.5
10	13811.0
11	13895.5
12	13980.0
13	13598.5
14	13217.0
15	12679.5
16	12142.0
17	11483.5
18	10825.0
19	10904.5
20	10984.0
21	11599.0
22	12214.0
23	11457.5
24	10701.0
25	11994.0
26	13852.0
27	14417.0
28	18186.5
29	21956.0
30	23680.0
31	25404.0
32	29072.0
33	32740.0
34	40044.0
35	47348.0
36	47101.5
37	46855.0
38	54956.5
39	63058.0
40	90975.0
41	118892.0
42	143851.0
43	168810.0
44	174313.5
45	179817.0
46	167838.5
47	155860.0
48	143735.5
49	131611.0
50	124989.5
51	118368.0
52	108710.0
53	99052.0
54	88232.0
55	77412.0
56	73682.5
57	69953.0
58	63426.0
59	56899.0
60	51209.5
61	45520.0
62	37178.0
63	28836.0
64	23786.0
65	18736.0
66	14333.5
67	9931.0
68	8085.5
69	6240.0
70	5130.0
71	4020.0
72	3351.5
73	2683.0
74	1993.5
75	1022.0
76	740.0
77	519.0
78	298.0
79	210.0
80	122.0
81	104.5
82	87.0
83	71.5
84	56.0
85	32.0
86	8.0
87	6.5
88	5.0
89	5.0
90	5.0
91	2.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1709368.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.73602969326951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.38400026087473	15.435490751380723
2	5.868725578134919	2.1991323338717224
3	2.0431539412186566	1.1484177873178014
4	1.0553209001085195	0.7909009488144452
5	0.6526009698295414	0.6113575574291384
6	0.5009222708514254	0.563117672441538
7	0.39852288904689587	0.5226715677841135
8	0.32852582206087777	0.4924215645710706
9	0.29401557658911026	0.4957816115931595
>10	3.611531132502695	16.051322375985784
>50	1.5100303958178594	20.813757785764274
>100	1.3429121862923346	38.212051218234556
>500	0.006910893122438618	0.8334565895385911
>1k	0.00188478903339235	0.45036436855796197
>5k	9.42394516696175E-4	1.3797558667151466
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9806	0.5736623126208049	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8483	0.4962652863514469	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5154	0.30151494587473265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02796355144123442	0.0
2	0.0	0.0	0.0	0.09161280660454624	0.0
3	0.0	0.0	0.0	0.13683419837039187	0.0
4	0.0	0.0	0.0	0.19428233124757222	0.0
5	0.0	0.0	0.0	0.30139794356744715	0.0
6	0.0	0.0	0.0	0.4153581908635238	0.0
7	0.0	0.0	0.0	0.4922287067500971	0.0
8	0.0	0.0	0.0	0.7064014302362043	0.0
9	0.0	0.0	0.0	0.7985407472235353	0.0
10	0.0	0.0	0.0	0.9466656682469778	0.0
11	0.0	0.0	0.0	1.0917485292809974	0.0
12	0.0	0.0	0.0	1.2385864249242995	0.0
13	0.0	0.0	0.0	1.2974970866425486	0.0
14	0.0	0.0	0.0	1.3204880400241492	0.0
15	0.0	0.0	0.0	1.3618483556495735	0.0
16	0.0	0.0	0.0	1.434448287320226	0.0
17	0.0	0.0	0.0	1.5405108788745314	0.0
18	0.0	0.0	0.0	1.7538060850559973	0.0
19	0.0	0.0	0.0	1.8126582456206037	0.0
20	0.0	0.0	0.0	1.8797590688488377	0.0
21	0.0	0.0	0.0	1.933697132507453	0.0
22	0.0	0.0	0.0	1.990560253848206	0.0
23	0.0	0.0	0.0	2.054619017087017	0.0
24	0.0	0.0	0.0	2.101185935386646	0.0
25	0.0	0.0	0.0	2.138802177178934	0.0
26	0.0	0.0	0.0	2.1837310631765656	0.0
27	0.0	0.0	0.0	2.232345521853691	0.0
28	0.0	0.0	0.0	2.2806674747626023	0.0
29	0.0	0.0	0.0	2.3293404345933704	0.0
30	5.850115364274984E-5	0.0	0.0	2.4005363385765968	0.0
31	5.850115364274984E-5	0.0	0.0	2.47208324948168	0.0
32	5.850115364274984E-5	0.0	0.0	2.5323394377337123	0.0
33	5.850115364274984E-5	0.0	0.0	2.5911330971446755	0.0
34	5.850115364274984E-5	0.0	0.0	2.6554843661517005	0.0
35	5.850115364274984E-5	0.0	0.0	2.7300733370462065	0.0
36	5.850115364274984E-5	0.0	0.0	2.7969986568135123	0.0
37	5.850115364274984E-5	0.0	0.0	2.8648014938854596	0.0
38	5.850115364274984E-5	0.0	0.0	2.933891356337547	0.0
39	5.850115364274984E-5	0.0	0.0	3.0037417337869905	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	30	2.1661253E-6	45.000004	31
TCTGCGA	20	7.0342526E-4	45.0	45
CTATGCG	50	2.1827873E-11	45.0	1
GTACCTC	20	7.0342526E-4	45.0	41
GATCGAC	20	7.0342526E-4	45.0	10
AACCCGC	20	7.0342526E-4	45.0	17
AAGCGTC	20	7.0342526E-4	45.0	36
TAACGCG	50	2.1827873E-11	45.0	1
GTAATCG	25	3.891607E-5	45.0	13
CTACGGC	20	7.0342526E-4	45.0	36
ACGTCCA	40	6.8175723E-9	45.0	10
GGTTCGC	20	7.0342526E-4	45.0	8
TCGACGC	20	7.0342526E-4	45.0	42
TATCGTG	50	2.1827873E-11	45.0	1
ATCGATC	20	7.0342526E-4	45.0	19
CGTTTTT	9115	0.0	42.85244	1
TGCGAAG	75	0.0	42.0	1
ACGGGTA	235	0.0	41.17021	5
CGTAAGG	225	0.0	41.0	2
TCGATTG	55	6.184564E-11	40.909092	1
>>END_MODULE