##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548912_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2205335
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90028181659476	31.0	31.0	33.0	28.0	34.0
2	31.180711773948175	31.0	31.0	34.0	28.0	34.0
3	31.257463378579672	31.0	31.0	34.0	28.0	34.0
4	35.10950354481292	35.0	35.0	37.0	32.0	37.0
5	34.98374850079466	35.0	35.0	37.0	32.0	37.0
6	35.04588418539587	37.0	35.0	37.0	32.0	37.0
7	35.53465165156314	37.0	35.0	37.0	35.0	37.0
8	35.439858343516974	37.0	35.0	37.0	35.0	37.0
9	37.0801569829527	39.0	37.0	39.0	33.0	39.0
10	36.51258289556915	38.0	35.0	39.0	32.0	39.0
11	36.26870747528153	38.0	35.0	39.0	32.0	39.0
12	35.69029467178456	37.0	35.0	39.0	30.0	39.0
13	35.300607390713886	37.0	35.0	39.0	30.0	39.0
14	36.341680062212774	38.0	35.0	40.0	30.0	41.0
15	36.62024272956263	38.0	35.0	40.0	31.0	41.0
16	36.6562785245779	38.0	35.0	40.0	31.0	41.0
17	36.54433408076324	38.0	35.0	40.0	31.0	41.0
18	36.460832934678855	38.0	35.0	40.0	31.0	41.0
19	36.366657673324006	38.0	35.0	40.0	30.0	41.0
20	36.187371079677234	37.0	34.0	40.0	30.0	41.0
21	36.05163977354914	37.0	34.0	40.0	30.0	41.0
22	35.9216282333523	37.0	34.0	40.0	30.0	41.0
23	35.85488735271512	37.0	34.0	40.0	30.0	41.0
24	35.757056864376615	37.0	34.0	40.0	30.0	41.0
25	35.640870888096366	37.0	34.0	40.0	29.0	41.0
26	35.498077162880016	37.0	34.0	40.0	29.0	41.0
27	35.37953054751319	37.0	34.0	40.0	29.0	41.0
28	35.356441084914536	37.0	34.0	40.0	28.0	41.0
29	35.35821677885673	37.0	34.0	40.0	29.0	41.0
30	35.24530286781827	37.0	34.0	40.0	28.0	41.0
31	35.08412599446343	37.0	34.0	40.0	27.0	41.0
32	34.83282630530056	37.0	34.0	40.0	25.0	41.0
33	34.553843747095115	36.0	33.0	40.0	24.0	41.0
34	34.3410615620756	36.0	33.0	40.0	23.0	41.0
35	34.13584285380679	36.0	33.0	40.0	23.0	41.0
36	33.96622689976806	36.0	33.0	40.0	22.0	41.0
37	33.901091670879936	36.0	33.0	40.0	22.0	41.0
38	33.84063192213428	36.0	33.0	40.0	23.0	41.0
39	33.77346888341227	36.0	33.0	40.0	22.0	41.0
40	33.64103186137253	36.0	32.0	40.0	21.0	41.0
41	33.578011050475325	36.0	32.0	40.0	21.0	41.0
42	33.51345759261065	36.0	32.0	40.0	21.0	41.0
43	33.428373467069626	35.0	32.0	40.0	21.0	41.0
44	33.359118229203276	35.0	32.0	40.0	21.0	41.0
45	33.30258577495029	35.0	32.0	40.0	20.0	41.0
46	33.227889640349424	35.0	32.0	39.0	20.0	41.0
47	33.126741288738444	35.0	32.0	39.0	20.0	41.0
48	33.08888037418352	35.0	32.0	39.0	20.0	41.0
49	33.10321153022103	35.0	32.0	39.0	20.0	41.0
50	33.0360144830604	35.0	32.0	39.0	20.0	41.0
51	32.859612711900915	35.0	31.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	1.0
12	11.0
13	8.0
14	27.0
15	97.0
16	306.0
17	791.0
18	1769.0
19	3438.0
20	5924.0
21	9248.0
22	13843.0
23	20118.0
24	27990.0
25	37829.0
26	48388.0
27	54450.0
28	58195.0
29	63601.0
30	73384.0
31	86151.0
32	105404.0
33	131676.0
34	200019.0
35	254114.0
36	173894.0
37	207594.0
38	273005.0
39	353884.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.20982979910082	23.120523639265688	26.390729753076066	17.27891680855743
2	34.394865179213134	25.778351134861598	27.351400127418284	12.475383558506985
3	29.986147229332506	24.66867845474724	32.61744814279916	12.72772617312109
4	27.288643222004822	26.838280805410513	31.74229765545824	14.130778317126422
5	23.343120206227173	31.406521004745308	31.081763088147607	14.168595700879912
6	20.273881292411357	40.71404117741749	28.818478825212495	10.193598704958658
7	81.85395869561766	5.425842332344065	10.624462950073346	2.0957360219649166
8	82.69927244613629	5.068663037588394	10.16806970369581	2.0639948125794945
9	76.6413719457588	6.881584883929199	12.321801449666376	4.155241720645616
10	42.877884765806556	25.60604171248359	18.695980429277185	12.82009309243267
11	38.0336320785731	23.095130671757353	24.122321552054448	14.7489156976151
12	34.59941460140976	21.642788964034942	28.597650697059628	15.160145737495665
13	22.799257255700383	32.61409264352128	28.012206762237934	16.574443338540405
14	17.3413109572922	35.9959824697835	30.171379858388864	16.491326714535433
15	17.238242715959252	23.04629455388864	42.44266744054758	17.272795289604527
16	18.46803320130502	20.324984639521887	41.2268430873314	19.980139071841695
17	18.40323578957392	21.135065647622696	31.208591891934784	29.2531066708686
18	21.409400385882417	23.927430526427955	33.92863215792612	20.734536929763507
19	27.699873261885383	24.09008155223583	27.770293402136183	20.439751783742608
20	28.770549599040507	22.754139393788243	28.414594608075415	20.060716399095828
21	23.226312555688818	26.793933801440595	30.02695735568519	19.952796287185393
22	22.611439985308355	24.062058598806985	29.040485912571107	24.286015503313553
23	21.35485085032433	27.682052840044708	27.516590449976984	23.446505859653975
24	20.198019801980198	24.23337044031859	36.84029863943573	18.728311118265477
25	19.85503336227829	24.71116633073887	34.2322594979901	21.20154080899274
26	18.426542906179787	29.877138847385996	29.546350101005064	22.149968145429153
27	19.62073789242904	29.38877766869886	30.234000730047818	20.756483708824284
28	18.30778543849347	27.85014521603294	35.39675378117157	18.44531556430202
29	18.863936771510904	24.922834852754796	35.371406158248064	20.841822217486232
30	19.77123656950078	28.47717013514954	32.456474866630245	19.295118428719444
31	26.442830681052993	26.70564789476429	27.15873098644877	19.69279043773395
32	26.93291495396391	26.22381633629358	28.42588540969966	18.41738330004285
33	25.708293751289485	27.200992139516217	26.744553548553846	20.34616056064045
34	20.089600899636565	28.653243158068953	28.9228620595057	22.334293882788785
35	22.099681000845678	25.208687115562945	30.581158871554663	22.110473012036717
36	26.325206827987586	28.05501205032342	26.390911131415407	19.228869990273587
37	21.187801399787336	31.809135573506975	27.921517592565305	19.08154543414039
38	21.643151720713632	31.47163582857026	25.484608914291933	21.40060353642417
39	21.90619565734911	30.23699347264701	26.839913210464623	21.016897659539254
40	23.179789011646758	27.460000408101266	27.362237483194164	21.997973097057816
41	19.306318541174015	26.703244631767966	28.241605016924865	25.748831810133154
42	21.47038885248726	28.064806480648063	25.606359124577445	24.858445542287228
43	22.49807852321756	27.21786032507533	26.542407389353546	23.741653762353565
44	20.784506662253126	29.376534630793056	27.824752248524597	22.014206458429218
45	19.500529398027965	33.16761399061821	25.744478729988867	21.587377881364965
46	21.670449160785097	31.41540854337323	26.938900439162307	19.97524185667937
47	22.230364094343944	28.78841536546602	27.466892784996382	21.514327755193655
48	21.875860130093614	26.84635214151138	29.770216316341962	21.507571412053046
49	21.23817923354048	27.017210537174623	30.91444157010159	20.830168659183297
50	20.013603375450895	30.057927707128396	29.016634660947204	20.911834256473504
51	19.79853400957224	30.85086846216108	27.186073771105068	22.16452375716161
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1817.0
1	3027.5
2	4238.0
3	20093.0
4	35948.0
5	26116.5
6	16285.0
7	16268.0
8	16251.0
9	16282.0
10	16313.0
11	16337.5
12	16362.0
13	15649.0
14	14936.0
15	13990.0
16	13044.0
17	12183.0
18	11322.0
19	11108.5
20	10895.0
21	10874.5
22	10854.0
23	10928.0
24	11002.0
25	13213.0
26	18332.5
27	21241.0
28	26383.5
29	31526.0
30	37873.0
31	44220.0
32	46780.5
33	49341.0
34	53995.0
35	58649.0
36	61728.5
37	64808.0
38	70867.0
39	76926.0
40	103330.5
41	129735.0
42	147972.0
43	166209.0
44	173422.5
45	180636.0
46	175995.0
47	171354.0
48	164498.5
49	157643.0
50	153818.5
51	149994.0
52	139161.5
53	128329.0
54	120835.5
55	113342.0
56	112579.0
57	111816.0
58	106802.5
59	101789.0
60	91924.5
61	82060.0
62	71266.5
63	60473.0
64	50018.5
65	39564.0
66	32558.0
67	25552.0
68	20714.0
69	15876.0
70	13877.5
71	11879.0
72	9913.5
73	7948.0
74	6489.5
75	3702.5
76	2374.0
77	1779.0
78	1184.0
79	947.0
80	710.0
81	464.5
82	219.0
83	175.0
84	131.0
85	93.0
86	55.0
87	32.5
88	10.0
89	10.0
90	10.0
91	7.5
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2205335.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.967126156326916
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4930617688515	15.672808667350191
2	5.991890971166219	2.3928168587246423
3	2.4238170574592393	1.4518998289843716
4	1.463807634456	1.1691212682310972
5	1.0343404452738718	1.0326403079687378
6	0.7728861706201272	0.9259389403951493
7	0.618230243362203	0.8640996884008859
8	0.5211056969470926	0.8323986553378608
9	0.4606054006188785	0.8277269528198381
>10	5.726910749272348	26.502961064402307
>50	1.7617383570153313	25.149208610005715
>100	0.7212979772168105	19.992505408717573
>500	0.006413572816256979	0.8866825686253178
>1k	0.0036648987521468446	1.3934367265852148
>5k	0.0	0.0
>10k+	2.2905617200917779E-4	0.9057544534510649
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19804	0.8980041580984295	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3702	0.16786565306404697	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3686	0.16714013970666589	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3080	0.13966132129585754	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3058	0.13866374042945856	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2350	0.10655977436534586	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2264	0.10266014006942256	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00934098447628138	0.0
2	0.0	0.0	0.0	0.0367744583022534	0.0
3	9.068916967263477E-5	0.0	0.0	0.05604590685768829	0.0
4	9.068916967263477E-5	0.0	0.0	0.07831009801232013	0.0
5	1.3603375450895215E-4	0.0	0.0	0.1416111384438192	0.0
6	1.3603375450895215E-4	0.0	0.0	0.2266775795967506	0.0
7	1.3603375450895215E-4	0.0	0.0	0.29233653843973817	0.0
8	1.3603375450895215E-4	0.0	0.0	0.45893254312836823	0.0
9	1.8137833934526955E-4	0.0	0.0	0.5476718956530414	0.0
10	1.8137833934526955E-4	0.0	0.0	0.6936814588259833	0.0
11	1.8137833934526955E-4	0.0	0.0	0.774077407740774	0.0
12	1.8137833934526955E-4	0.0	0.0	0.8707067180269664	0.0
13	2.2672292418158692E-4	0.0	0.0	0.9097030609861994	0.0
14	2.2672292418158692E-4	0.0	0.0	0.927070036978509	0.0
15	2.2672292418158692E-4	0.0	0.0	0.9507852548479029	0.0
16	2.2672292418158692E-4	0.0	0.0	0.9912779691067344	0.0
17	2.2672292418158692E-4	0.0	0.0	1.036667898527888	0.0
18	2.2672292418158692E-4	0.0	0.0	1.1206007250599115	0.0
19	2.2672292418158692E-4	0.0	0.0	1.1561508795715845	0.0
20	2.2672292418158692E-4	0.0	0.0	1.1943310200037636	0.0
21	2.2672292418158692E-4	0.0	0.0	1.2367282068257204	0.0
22	2.2672292418158692E-4	0.0	0.0	1.2806671095321118	0.0
23	2.2672292418158692E-4	0.0	0.0	1.3295032274008258	0.0
24	2.2672292418158692E-4	0.0	0.0	1.3715830021289284	0.0
25	2.2672292418158692E-4	0.0	0.0	1.4055914407561663	0.0
26	2.2672292418158692E-4	0.0	0.0	1.4386476431018416	0.0
27	2.2672292418158692E-4	0.0	0.0	1.4752407230647497	0.0
28	2.2672292418158692E-4	0.0	0.0	1.5159148156629265	0.0
29	2.2672292418158692E-4	0.0	0.0	1.5598990629541543	0.0
30	2.2672292418158692E-4	0.0	0.0	1.6180761652991495	0.0
31	2.2672292418158692E-4	0.0	0.0	1.6657786685469556	0.0
32	2.2672292418158692E-4	0.0	0.0	1.7150228876791962	0.0
33	2.2672292418158692E-4	0.0	0.0	1.7668517481471069	0.0
34	2.2672292418158692E-4	0.0	0.0	1.8165494131277107	0.0
35	2.2672292418158692E-4	0.0	0.0	1.879442352295683	0.0
36	2.2672292418158692E-4	0.0	0.0	1.9329489624025376	0.0
37	2.2672292418158692E-4	0.0	0.0	1.987317119621282	0.0
38	2.2672292418158692E-4	0.0	0.0	2.047580072868748	0.0
39	2.2672292418158692E-4	0.0	0.0	2.1063919994014513	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	645	0.0	43.604652	31
CGTTTTT	13840	0.0	42.902817	1
GCGTAAG	175	0.0	42.42857	1
TAATACG	75	0.0	42.0	1
TCACGAC	685	0.0	41.386864	25
CGACGGT	680	0.0	41.360294	28
CACGACG	710	0.0	40.880283	26
CTCACGA	695	0.0	40.791367	24
ACGGGTA	305	0.0	40.57377	5
ATTGGGC	555	0.0	40.135136	4
CATATGC	1250	0.0	39.96	33
ACGGTCT	705	0.0	39.893616	30
TATAGCG	140	0.0	38.57143	1
ATACCGG	105	0.0	38.57143	2
ACGACGG	735	0.0	38.57143	27
AGGGCGA	1610	0.0	38.152176	6
CGGGTAC	195	0.0	38.076927	6
GGTACCT	1395	0.0	38.064518	8
CGCATCG	125	0.0	37.800003	21
ATAGGGC	880	0.0	37.585228	4
>>END_MODULE