FastQCFastQC Report
Sat 18 Jun 2016
SRR3548910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446472
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127990.5231615158481274No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC71910.29393346827595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT600.045.00000423
GGTATCG600.045.0000041
CACGTCA453.8562575E-1045.00000433
TACACCG302.166511E-645.00000435
GTACGAC302.166511E-645.00000438
ACGCGAT551.8189894E-1245.022
GCGCCTA207.0348504E-445.040
TGCTCGA207.0348504E-445.035
CCGTCGA4600.044.51086841
TCCGTCG1100.042.95454440
CGTTTTT134050.042.935471
ACGTTAG950.042.631581
CGATGAA34500.042.58695619
CCCGTCG5050.042.3267340
GATGAAT36050.042.31622720
TAGTAGG12850.041.4980552
CGTCGAA5450.041.28440542
GACACGA3150.040.71428325
GTCGAAT5400.040.41666843
CGTTAGG3400.040.3676452