##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548910_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2446472
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.904397025594406	31.0	31.0	33.0	28.0	34.0
2	31.1820163893149	31.0	31.0	34.0	28.0	34.0
3	31.260141951348718	31.0	31.0	34.0	28.0	34.0
4	35.092787491538836	35.0	35.0	37.0	32.0	37.0
5	34.93280895918694	35.0	35.0	37.0	32.0	37.0
6	34.989889931296986	36.0	35.0	37.0	32.0	37.0
7	35.538520776039945	37.0	35.0	37.0	35.0	37.0
8	35.464816682962244	37.0	35.0	37.0	35.0	37.0
9	37.12735808952647	39.0	37.0	39.0	34.0	39.0
10	36.55545659218662	39.0	35.0	39.0	32.0	39.0
11	36.19302285086443	38.0	35.0	39.0	31.0	39.0
12	35.14276231242377	37.0	34.0	39.0	30.0	39.0
13	34.51615714383815	35.0	33.0	39.0	27.0	39.0
14	35.44165802837719	37.0	33.0	40.0	27.0	41.0
15	35.88825173556043	37.0	34.0	40.0	30.0	41.0
16	36.04883644693256	37.0	34.0	40.0	31.0	41.0
17	35.906959082303004	37.0	34.0	40.0	30.0	41.0
18	35.872447344584366	36.0	34.0	40.0	30.0	41.0
19	35.76758736662426	36.0	34.0	40.0	30.0	41.0
20	35.49558139230696	36.0	34.0	40.0	29.0	41.0
21	35.365183415138205	35.0	34.0	40.0	29.0	41.0
22	35.23107478851178	35.0	34.0	40.0	29.0	41.0
23	35.12203981897197	35.0	34.0	40.0	29.0	41.0
24	35.10604249711421	35.0	34.0	40.0	29.0	41.0
25	35.04452125346213	35.0	34.0	40.0	29.0	41.0
26	34.81503242219817	35.0	34.0	40.0	27.0	41.0
27	34.6644179863902	35.0	33.0	40.0	27.0	41.0
28	34.739637322642565	35.0	33.0	40.0	27.0	41.0
29	34.774116360211764	35.0	34.0	40.0	27.0	41.0
30	34.70049974003381	36.0	34.0	40.0	27.0	41.0
31	34.491628352991576	35.0	33.0	40.0	26.0	41.0
32	34.1237598468325	35.0	33.0	40.0	24.0	41.0
33	33.809635671285015	35.0	33.0	40.0	23.0	41.0
34	33.57181484194383	35.0	33.0	40.0	23.0	41.0
35	33.35975682533869	35.0	32.0	40.0	22.0	41.0
36	33.194055358082984	35.0	32.0	39.0	20.0	41.0
37	33.15636189582386	35.0	32.0	39.0	21.0	41.0
38	33.11535876968958	35.0	32.0	39.0	21.0	41.0
39	33.05779914914211	35.0	32.0	39.0	20.0	41.0
40	32.91341000428372	35.0	31.0	39.0	18.0	41.0
41	32.883873185550456	35.0	32.0	39.0	18.0	41.0
42	32.87721829638762	35.0	32.0	39.0	19.0	41.0
43	32.72340905597939	35.0	31.0	39.0	18.0	41.0
44	32.73222828628327	35.0	31.0	39.0	18.0	41.0
45	32.70180284098898	35.0	31.0	39.0	20.0	41.0
46	32.6416239384714	35.0	31.0	39.0	20.0	40.0
47	32.48271020473564	35.0	31.0	38.0	18.0	40.0
48	32.56241314022805	35.0	31.0	38.0	20.0	40.0
49	32.61442558917494	35.0	31.0	38.0	19.0	40.0
50	32.46063719511198	35.0	31.0	38.0	18.0	40.0
51	32.31361323571249	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	4.0
12	5.0
13	16.0
14	39.0
15	107.0
16	386.0
17	986.0
18	2277.0
19	4482.0
20	8325.0
21	13396.0
22	20777.0
23	29754.0
24	39099.0
25	48999.0
26	59426.0
27	65522.0
28	68512.0
29	74113.0
30	86084.0
31	103117.0
32	126721.0
33	160536.0
34	265547.0
35	349556.0
36	159711.0
37	186059.0
38	246747.0
39	326019.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.485361778103325	23.04150629968379	27.556906435062405	15.916225487150475
2	32.48522770749062	26.990008469338704	27.695514193499864	12.829249629670809
3	28.424686650817993	26.125539143713887	32.479300805404684	12.970473400063437
4	26.37389677870828	26.027683946515634	33.03303696097891	14.565382313797173
5	24.866174638418098	30.432475826414528	30.687250865736456	14.014098669430918
6	21.20997092956715	40.37422051018773	28.407600822735763	10.00820773750936
7	81.89621626570833	5.444820132828007	10.821828330755471	1.8371352707081874
8	83.49059380201368	5.585144649111046	9.04175482081953	1.882506728055747
9	78.7126523418212	5.466238730711	10.825711473501434	4.995397453966365
10	53.81962270567576	20.482760481215397	15.2433790372422	10.454237775866636
11	50.21434947957712	19.568627803629063	19.967487876419597	10.24953484037422
12	43.46340362775457	20.36802383186891	22.924848516557724	13.243724023818789
13	23.16000346621584	39.6141464116491	23.427776814940042	13.798073307195013
14	14.519029851966422	43.92848150316047	28.08444159589809	13.468047048975013
15	14.157488824723929	21.742206736884786	49.97216399778947	14.128140440601813
16	16.29714135293598	16.951880095092033	48.47654091279197	18.27443763918001
17	15.617100870150976	18.511104970749717	30.24040332364319	35.631390835456116
18	21.433026823932586	22.820248913537537	34.583841548155874	21.162882714374003
19	30.42597667171339	22.834432603356998	26.22490672282372	20.51468400210589
20	34.32992488775673	21.52311573563891	25.47337553832621	18.67358383827814
21	23.161556723314224	28.761784316354326	28.867078797550107	19.209580162781347
22	21.733868198777667	24.70308264308768	27.856971181358297	25.706077976776353
23	21.081132340774797	29.75852574646266	25.935101648414534	23.225240264348006
24	20.340964458207576	23.304047624497645	39.729291812863586	16.6256961044312
25	17.635558469502204	24.39578298872826	37.31614340977538	20.652515131994154
26	15.671669244528447	32.77454227965822	30.316880798145245	21.23690767766809
27	19.158894931149835	33.52819897386931	29.279509432358108	18.033396662622746
28	15.13620429745364	30.516147333793313	38.881499563453005	15.46614880530004
29	16.97174543587664	24.269683037451482	38.92094411871462	19.837627407957253
30	18.538900097773446	30.47956404160767	32.87006759120889	18.11146826940999
31	29.10395050505381	26.53801065370869	25.7738490364901	18.58418980474741
32	32.97017092368112	24.60490044439503	27.797252533444077	14.62767609847977
33	30.58473589724305	26.161795434405132	25.052034112795894	18.201434555555917
34	20.548978283830756	27.600806385685182	28.53942330016448	23.31079203031958
35	21.350704197718184	25.00829766292032	31.50667573550811	22.134322403853385
36	30.28847254331952	28.20036362566177	24.683626054171068	16.827537776847638
37	21.718458253354218	32.68604750023707	27.857993061028292	17.737501185380417
38	20.916405337972392	33.76372180020863	23.315574427175132	22.004298434643847
39	21.675661932775032	32.228081907334314	26.112540834311616	19.983715325579038
40	24.442544202427005	25.04210144240359	25.865409454921206	24.649944900248194
41	17.58143972217953	24.74628771553486	27.604403402123545	30.067869160162065
42	22.69586572010634	25.43421710937219	24.384828438665966	27.485088731855505
43	22.721944089284488	26.333879970831465	25.425224568276278	25.518951371607766
44	20.037466196220517	29.777042206082882	28.480154279305058	21.705337318391546
45	18.09515906987695	36.86443172045296	23.303720623003247	21.73668858666684
46	22.129294755877034	33.50163010244957	25.039648931195618	19.32942621047778
47	22.05952081201011	28.501777253122047	26.3617977234156	23.076904211452245
48	23.576235493396204	24.834782494956002	30.381504468475423	21.207477543172374
49	21.596077944076207	24.66163520367288	32.06760592395908	21.674680928291842
50	19.453932029469374	33.155008518388925	26.72530075962447	20.665758692517226
51	18.146089552629256	33.71659270982869	24.729692389694222	23.40762534784784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2247.0
1	3376.5
2	4506.0
3	15917.0
4	27328.0
5	20516.0
6	13704.0
7	14438.5
8	15173.0
9	16000.0
10	16827.0
11	16920.0
12	17013.0
13	16531.5
14	16050.0
15	15225.0
16	14400.0
17	13541.5
18	12683.0
19	12161.5
20	11640.0
21	11493.5
22	11347.0
23	11527.0
24	11707.0
25	12460.5
26	14308.5
27	15403.0
28	20092.0
29	24781.0
30	28717.5
31	32654.0
32	36218.5
33	39783.0
34	45906.5
35	52030.0
36	54281.5
37	56533.0
38	71449.5
39	86366.0
40	125766.5
41	165167.0
42	203674.0
43	242181.0
44	241055.5
45	239930.0
46	229220.5
47	218511.0
48	198505.0
49	178499.0
50	171587.5
51	164676.0
52	158895.0
53	153114.0
54	145727.5
55	138341.0
56	130156.5
57	121972.0
58	115504.0
59	109036.0
60	97926.5
61	86817.0
62	72606.5
63	58396.0
64	45071.5
65	31747.0
66	24204.0
67	16661.0
68	13272.5
69	9884.0
70	8307.0
71	6730.0
72	5542.5
73	4355.0
74	3402.0
75	1835.5
76	1222.0
77	942.5
78	663.0
79	525.0
80	387.0
81	315.0
82	243.0
83	148.0
84	53.0
85	37.0
86	21.0
87	22.0
88	23.0
89	13.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2446472.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.2440431528665
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.34178996639673	14.199053794648151
2	6.214087025137363	2.14311969634273
3	2.1752689011999897	1.1253129240414332
4	1.1797440973910909	0.8137423249900603
5	0.7346920180899628	0.6334530432004947
6	0.49833483880846435	0.5155984478993958
7	0.3662244813129306	0.4420633531577428
8	0.2972900213348636	0.4101185565451992
9	0.2507100027401967	0.38909286954965067
>10	3.2170177731982705	13.486085017302102
>50	1.1240115445572971	13.956605083956639
>100	1.5793928412857845	48.58903643889506
>500	0.018816473280199142	1.9705312467332137
>1k	0.0021436488547062313	0.5051496301055046
>5k	2.3818320607847015E-4	0.2953517351076253
>10k+	2.3818320607847015E-4	0.5256858375250308
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12799	0.5231615158481274	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7191	0.29393346827595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011526802677488237	0.0
2	0.0	0.0	0.0	0.04990860308231609	0.0
3	0.0	0.0	0.0	0.07349358586568741	0.0
4	0.0	0.0	0.0	0.12082705217962846	0.0
5	0.0	0.0	0.0	0.23343819181253658	0.0
6	0.0	0.0	0.0	0.32320010202446625	0.0
7	0.0	0.0	0.0	0.39223829252899683	0.0
8	0.0	0.0	0.0	0.5625651959229454	0.0
9	0.0	0.0	0.0	0.6387565441174066	0.0
10	0.0	0.0	0.0	0.8343443129535102	0.0
11	0.0	0.0	0.0	0.9189150744418902	0.0
12	0.0	0.0	0.0	1.0477127880474413	0.0
13	0.0	0.0	0.0	1.0880565974186502	0.0
14	0.0	0.0	0.0	1.1053059262480829	0.0
15	0.0	0.0	0.0	1.133141928458613	0.0
16	0.0	0.0	0.0	1.1852169164413082	0.0
17	0.0	0.0	0.0	1.2336948879856382	0.0
18	0.0	0.0	0.0	1.3277895679983258	4.0875186799603676E-5
19	0.0	0.0	0.0	1.378393049256235	4.0875186799603676E-5
20	0.0	0.0	0.0	1.4160799714854697	4.0875186799603676E-5
21	0.0	0.0	0.0	1.4604295491630397	4.0875186799603676E-5
22	0.0	0.0	0.0	1.503307620115824	4.0875186799603676E-5
23	0.0	0.0	0.0	1.5526439705829456	4.0875186799603676E-5
24	0.0	0.0	0.0	1.5900038913177834	4.0875186799603676E-5
25	0.0	0.0	0.0	1.622744915944266	4.0875186799603676E-5
26	0.0	0.0	0.0	1.6577340758447265	4.0875186799603676E-5
27	0.0	0.0	0.0	1.6950122462059651	4.0875186799603676E-5
28	0.0	0.0	0.0	1.7302875324140232	4.0875186799603676E-5
29	0.0	0.0	0.0	1.7688328335660495	4.0875186799603676E-5
30	0.0	0.0	0.0	1.8261807206458933	4.0875186799603676E-5
31	0.0	0.0	0.0	1.8800950920345707	4.0875186799603676E-5
32	0.0	0.0	0.0	1.9272650576013133	4.0875186799603676E-5
33	0.0	0.0	0.0	1.9750890261568494	4.0875186799603676E-5
34	0.0	0.0	0.0	2.023035620272785	4.0875186799603676E-5
35	0.0	0.0	0.0	2.090765804799728	4.0875186799603676E-5
36	0.0	0.0	0.0	2.147091812209582	4.0875186799603676E-5
37	0.0	0.0	0.0	2.203785696300632	4.0875186799603676E-5
38	4.0875186799603676E-5	0.0	0.0	2.2608065818860794	4.0875186799603676E-5
39	8.175037359920735E-5	0.0	0.0	2.3180727185923238	4.0875186799603676E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	60	0.0	45.000004	23
GGTATCG	60	0.0	45.000004	1
CACGTCA	45	3.8562575E-10	45.000004	33
TACACCG	30	2.166511E-6	45.000004	35
GTACGAC	30	2.166511E-6	45.000004	38
ACGCGAT	55	1.8189894E-12	45.0	22
GCGCCTA	20	7.0348504E-4	45.0	40
TGCTCGA	20	7.0348504E-4	45.0	35
CCGTCGA	460	0.0	44.510868	41
TCCGTCG	110	0.0	42.954544	40
CGTTTTT	13405	0.0	42.93547	1
ACGTTAG	95	0.0	42.63158	1
CGATGAA	3450	0.0	42.586956	19
CCCGTCG	505	0.0	42.32673	40
GATGAAT	3605	0.0	42.316227	20
TAGTAGG	1285	0.0	41.498055	2
CGTCGAA	545	0.0	41.284405	42
GACACGA	315	0.0	40.714283	25
GTCGAAT	540	0.0	40.416668	43
CGTTAGG	340	0.0	40.367645	2
>>END_MODULE