FastQCFastQC Report
Sat 18 Jun 2016
SRR3548909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3536274
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169350.47889388661625204No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC128000.36196290219592714No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT43660.123463283670892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC302.1667856E-645.00000418
ATCCGAT302.1667856E-645.00000427
ATTCACG351.212884E-745.0000041
GTTCGAC207.035276E-445.00000431
ACGTATG406.8212103E-945.0000041
GCGTATT302.1667856E-645.00000416
AGTCGTA302.1667856E-645.00000442
CATATCG253.8924558E-545.037
ACGTTAG1000.045.01
CACGATA551.8189894E-1245.035
TAAGTCG1250.044.9999961
GCGTAAG3750.043.81
GCTACGA4500.042.510
CGTTTTT174750.042.2703821
CGACGGT3550.041.83098628
CGGTCTA3500.041.78571331
GTCGACC650.041.53846431
TACGTAG1300.041.5384641
AATGCGG5400.041.2500042
GCGATAC2400.041.2500049
Produced by FastQC (version 0.11.5)