##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548909_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3536274
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.889650801945777	31.0	31.0	33.0	28.0	34.0
2	31.16713637008897	31.0	31.0	34.0	28.0	34.0
3	31.261547040755325	31.0	31.0	34.0	28.0	34.0
4	35.092451263674704	35.0	35.0	37.0	32.0	37.0
5	34.94330840879412	35.0	35.0	37.0	32.0	37.0
6	34.98925309520699	36.0	35.0	37.0	32.0	37.0
7	35.51320316242463	37.0	35.0	37.0	35.0	37.0
8	35.453273134378165	37.0	35.0	37.0	35.0	37.0
9	37.14017239614351	39.0	37.0	39.0	34.0	39.0
10	36.55644924573152	39.0	35.0	39.0	32.0	39.0
11	36.250059243146886	38.0	35.0	39.0	32.0	39.0
12	35.21725239616613	37.0	35.0	39.0	30.0	39.0
13	34.589873409130625	37.0	33.0	39.0	27.0	39.0
14	35.52436491063758	37.0	33.0	40.0	27.0	41.0
15	35.974805968089576	37.0	34.0	40.0	30.0	41.0
16	36.145569319571955	37.0	34.0	40.0	31.0	41.0
17	36.049450070893826	37.0	34.0	40.0	31.0	41.0
18	36.01132208646728	37.0	34.0	40.0	30.0	41.0
19	35.909182376704976	37.0	34.0	40.0	30.0	41.0
20	35.718875290772154	36.0	34.0	40.0	30.0	41.0
21	35.52250956798031	36.0	34.0	40.0	29.0	41.0
22	35.403117801392085	36.0	34.0	40.0	29.0	41.0
23	35.39956151587801	35.0	34.0	40.0	29.0	41.0
24	35.30569548626605	35.0	34.0	40.0	29.0	41.0
25	35.18427248567277	35.0	34.0	40.0	29.0	41.0
26	35.02030329097802	35.0	34.0	40.0	29.0	41.0
27	34.90534472159114	35.0	34.0	40.0	27.0	41.0
28	34.920947584943924	36.0	34.0	40.0	27.0	41.0
29	34.97214525797492	36.0	34.0	40.0	29.0	41.0
30	34.88364985292429	36.0	34.0	40.0	27.0	41.0
31	34.68778918149442	35.0	33.0	40.0	27.0	41.0
32	34.42053217595695	35.0	33.0	40.0	25.0	41.0
33	34.1821349250652	35.0	33.0	40.0	24.0	41.0
34	34.033761241351776	35.0	33.0	40.0	23.0	41.0
35	33.85360721482555	35.0	33.0	40.0	23.0	41.0
36	33.62679532185572	35.0	33.0	40.0	22.0	41.0
37	33.561440092029066	35.0	33.0	40.0	23.0	41.0
38	33.565983292018664	35.0	32.0	40.0	23.0	41.0
39	33.5505763976434	35.0	33.0	40.0	23.0	41.0
40	33.366926884059325	35.0	32.0	39.0	22.0	41.0
41	33.35838201451585	35.0	32.0	39.0	22.0	41.0
42	33.32046159319103	35.0	32.0	39.0	22.0	41.0
43	33.1985355207204	35.0	32.0	39.0	22.0	41.0
44	33.12835176233516	35.0	32.0	39.0	22.0	41.0
45	33.08542861780507	35.0	32.0	39.0	22.0	41.0
46	33.03109487556677	35.0	32.0	39.0	22.0	41.0
47	32.9521142875241	35.0	32.0	39.0	22.0	41.0
48	32.94406230965134	35.0	32.0	39.0	22.0	40.0
49	32.9790463069321	35.0	32.0	39.0	21.0	40.0
50	32.864035988161554	35.0	32.0	39.0	21.0	40.0
51	32.666256347783005	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	7.0
12	7.0
13	26.0
14	54.0
15	151.0
16	498.0
17	1405.0
18	3268.0
19	6017.0
20	10571.0
21	16232.0
22	23740.0
23	34083.0
24	46424.0
25	62221.0
26	77688.0
27	88850.0
28	96336.0
29	106314.0
30	123649.0
31	148232.0
32	182004.0
33	232217.0
34	380104.0
35	504378.0
36	239605.0
37	283098.0
38	374211.0
39	494642.0
40	238.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.73720475279913	22.70191732880427	28.01451471237806	15.546363206018537
2	31.92371405609407	25.285031646303423	29.697755320996055	13.093498976606451
3	28.911249524216732	24.53797415019311	33.17237295526308	13.378403370327074
4	26.46474792394481	27.207309162129405	31.74561699687298	14.582325917052808
5	23.711426207358365	31.92741852017123	29.90387622678559	14.457279045684809
6	20.51305979118134	40.60050211041339	28.61226251133255	10.274175587072722
7	83.55656264192199	5.51046101065698	9.004986604544783	1.927989742876259
8	85.30430617084535	4.454094903279553	8.383315320023279	1.858283605851809
9	80.48380866414763	6.010054650742561	10.273327236520698	3.2328094485891077
10	52.281638809662375	21.66814562446236	15.17817906644112	10.87203649943415
11	48.340400093431676	20.369038145799788	20.145921950618078	11.144639810150458
12	42.788002287153084	21.062083990098053	23.67834053582952	12.47157318691934
13	21.86731005572532	41.15190169087576	23.857681842526908	13.123106410872007
14	14.885526404345365	43.60977118854478	27.725736184469866	13.778966222639987
15	14.197316158193624	22.306190074637883	49.49984079287974	13.996652974288756
16	14.862536104385576	16.958159916341327	48.455662655099694	19.72364132417341
17	15.086217866601967	17.97895185723731	30.80202495621097	36.13280531994975
18	21.666533758413518	22.152751738128888	35.3268440171774	20.853870486280194
19	29.990549374850477	23.5525301489647	26.326579897372206	20.130340578812614
20	32.24379671937186	21.806511599497096	25.90571318851424	20.043978492616805
21	21.47647495640892	27.88672484089185	29.394498277000032	21.2423019256992
22	21.78945975340146	24.81196310014439	26.779853597317405	26.618723549136746
23	19.264400892012326	29.155970380123257	25.45724680836383	26.122381919500583
24	19.12807095830244	23.462972609022945	40.325438583096215	17.083517849578396
25	16.889924253607045	24.649249464266624	36.976518222287076	21.484308059839254
26	16.000117637943216	34.17498191599407	28.785382580648445	21.039517865414275
27	17.539704219752203	34.63481619354156	29.27343865322653	18.552040933479702
28	14.835530278479553	30.026915335180476	39.00851574284119	16.129038643498777
29	16.038406526191125	23.92280688657044	38.723837575934446	21.314949011303987
30	18.57285380035597	30.818200173402854	32.85743695200089	17.75150907424029
31	29.829108264800748	27.567547084869553	24.70167752838157	17.90166712194813
32	30.589993874909016	26.239793635900387	27.010095937136093	16.160116552054507
33	28.585539468943864	27.707751152767006	24.112441513299025	19.5942678649901
34	19.86486341273329	28.70371470084049	27.92091336813833	23.510508518287892
35	20.815440206273607	25.433775776424568	30.874474093353626	22.876309923948202
36	30.540506759374413	25.88439131130676	25.649228538286344	17.925873391032482
37	20.608018496304304	33.542310352648016	28.45237671062819	17.397294440419493
38	20.750428275637013	33.78103619798692	22.858013830376265	22.6105216959998
39	20.930165479258676	31.77833504982928	26.93841031549026	20.35308915542178
40	23.739506610630283	26.37338056949207	25.91750526118734	23.969607558690306
41	17.01714855805857	24.93158052797945	28.05868549778665	29.99258541617533
42	22.058641383557948	25.544287575001256	24.532770933474048	27.86430010796675
43	22.665777595288148	26.252660285939385	25.608140093216758	25.473422025555713
44	19.111499844186284	30.593952844151783	28.453366452938884	21.841180858723053
45	17.24020819653681	38.3337942704666	23.32751930421681	21.098478228779783
46	21.712061904705347	33.899437656697415	25.00318131457008	19.385319124027152
47	21.573752486374076	28.88619490458036	26.359411063735443	23.18064154531012
48	22.747869650372117	25.20036626121166	30.91437484765038	21.13738924076585
49	21.259862782126046	25.297163059197338	32.36284858017224	21.08012557850438
50	19.387581392165878	33.96312050480251	26.820433032055774	19.828865070975834
51	18.699625651179744	34.58654504713153	24.403369195939003	22.310460105749723
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3932.0
1	5273.0
2	6614.0
3	20114.5
4	33615.0
5	26317.0
6	19019.0
7	19743.5
8	20468.0
9	21549.0
10	22630.0
11	22785.0
12	22940.0
13	21891.5
14	20843.0
15	19340.5
16	17838.0
17	17289.5
18	16741.0
19	16030.0
20	15319.0
21	15501.0
22	15683.0
23	16180.5
24	16678.0
25	17772.0
26	23657.0
27	28448.0
28	32449.5
29	36451.0
30	45550.0
31	54649.0
32	60480.5
33	66312.0
34	74101.0
35	81890.0
36	90165.0
37	98440.0
38	115636.0
39	132832.0
40	189426.5
41	246021.0
42	312155.5
43	378290.0
44	367382.5
45	356475.0
46	332355.0
47	308235.0
48	292899.0
49	277563.0
50	261310.0
51	245057.0
52	226798.0
53	208539.0
54	188157.0
55	167775.0
56	160012.0
57	152249.0
58	142472.5
59	132696.0
60	122330.5
61	111965.0
62	95146.5
63	78328.0
64	63526.0
65	48724.0
66	39021.5
67	29319.0
68	23590.0
69	17861.0
70	14374.5
71	10888.0
72	9093.0
73	7298.0
74	5717.5
75	3161.5
76	2186.0
77	1693.0
78	1200.0
79	968.5
80	737.0
81	514.5
82	292.0
83	215.0
84	138.0
85	101.5
86	65.0
87	40.0
88	15.0
89	13.0
90	11.0
91	6.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3536274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.34106468989554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.74421429588425	12.693843442277394
2	6.70822152281467	2.0582252067129883
3	2.327029733358252	1.0709734102427793
4	1.240335130609392	0.7611224590331486
5	0.7727277418728019	0.5927233137873777
6	0.5313837163699384	0.4891195180792997
7	0.36961045917199553	0.39691525749537043
8	0.30431785977116294	0.3734847978431976
9	0.24419338213561576	0.3371567824968177
>10	2.303063834840991	8.202089289887684
>50	0.7516597754512532	8.378368012746435
>100	1.658263864582159	58.77076429666157
>500	0.04051798728922069	4.006939889452994
>1k	0.004088971194325023	1.020432542491185
>5k	0.0	0.0
>10k+	3.717246540295476E-4	0.8478417807917566
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16935	0.47889388661625204	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	12800	0.36196290219592714	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4366	0.123463283670892	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010576103548537245	0.0
2	0.0	0.0	0.0	0.03834544495138103	0.0
3	0.0	0.0	0.0	0.055029672474474545	0.0
4	0.0	0.0	0.0	0.08370392113280815	0.0
5	0.0	0.0	0.0	0.14557695472692445	0.0
6	0.0	0.0	0.0	0.22028836000830251	0.0
7	0.0	0.0	0.0	0.27676022842121395	0.0
8	0.0	0.0	0.0	0.45878797853333764	0.0
9	0.0	0.0	0.0	0.5422373945005393	0.0
10	2.8278351734056807E-5	0.0	0.0	0.682526297453195	0.0
11	2.8278351734056807E-5	0.0	0.0	0.7615642905498838	0.0
12	2.8278351734056807E-5	0.0	0.0	0.8616413773367109	0.0
13	2.8278351734056807E-5	0.0	0.0	0.921874266530252	0.0
14	2.8278351734056807E-5	0.0	0.0	0.9508595770576601	0.0
15	2.8278351734056807E-5	0.0	0.0	0.9769887740599286	0.0
16	5.6556703468113614E-5	0.0	0.0	1.0223189718896217	0.0
17	5.6556703468113614E-5	0.0	0.0	1.069204479064688	0.0
18	5.6556703468113614E-5	0.0	0.0	1.1607415036278297	0.0
19	5.6556703468113614E-5	0.0	0.0	1.1936009483428038	0.0
20	5.6556703468113614E-5	0.0	0.0	1.2312959912043016	0.0
21	5.6556703468113614E-5	0.0	0.0	1.27331762188111	0.0
22	5.6556703468113614E-5	0.0	0.0	1.313218376177864	0.0
23	5.6556703468113614E-5	0.0	0.0	1.3597928214838555	0.0
24	5.6556703468113614E-5	0.0	0.0	1.3962718952207889	0.0
25	5.6556703468113614E-5	0.0	0.0	1.4258793294863463	0.0
26	5.6556703468113614E-5	0.0	0.0	1.457522805076756	0.0
27	8.483505520217041E-5	0.0	0.0	1.4891945590188995	0.0
28	8.483505520217041E-5	0.0	0.0	1.522251952196012	0.0
29	8.483505520217041E-5	0.0	0.0	1.5602297785748502	0.0
30	8.483505520217041E-5	0.0	0.0	1.6107066364201417	0.0
31	8.483505520217041E-5	0.0	0.0	1.657620421946942	0.0
32	8.483505520217041E-5	0.0	0.0	1.7016498155968682	0.0
33	8.483505520217041E-5	0.0	0.0	1.7486201578271368	0.0
34	8.483505520217041E-5	0.0	0.0	1.799068737320694	0.0
35	8.483505520217041E-5	0.0	0.0	1.857859430575798	0.0
36	8.483505520217041E-5	0.0	0.0	1.9118710823878466	0.0
37	8.483505520217041E-5	0.0	0.0	1.961867208253659	0.0
38	8.483505520217041E-5	0.0	0.0	2.0137862620373874	0.0
39	8.483505520217041E-5	0.0	0.0	2.065903264283254	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGC	30	2.1667856E-6	45.000004	18
ATCCGAT	30	2.1667856E-6	45.000004	27
ATTCACG	35	1.212884E-7	45.000004	1
GTTCGAC	20	7.035276E-4	45.000004	31
ACGTATG	40	6.8212103E-9	45.000004	1
GCGTATT	30	2.1667856E-6	45.000004	16
AGTCGTA	30	2.1667856E-6	45.000004	42
CATATCG	25	3.8924558E-5	45.0	37
ACGTTAG	100	0.0	45.0	1
CACGATA	55	1.8189894E-12	45.0	35
TAAGTCG	125	0.0	44.999996	1
GCGTAAG	375	0.0	43.8	1
GCTACGA	450	0.0	42.5	10
CGTTTTT	17475	0.0	42.270382	1
CGACGGT	355	0.0	41.830986	28
CGGTCTA	350	0.0	41.785713	31
GTCGACC	65	0.0	41.538464	31
TACGTAG	130	0.0	41.538464	1
AATGCGG	540	0.0	41.250004	2
GCGATAC	240	0.0	41.250004	9
>>END_MODULE