##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548908_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4516102
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.962791141564118	31.0	31.0	33.0	30.0	34.0
2	31.247568589017696	31.0	31.0	34.0	30.0	34.0
3	31.312869594176572	31.0	31.0	34.0	28.0	34.0
4	35.148439738517865	35.0	35.0	37.0	32.0	37.0
5	35.03131771603033	35.0	35.0	37.0	32.0	37.0
6	35.07495468437161	37.0	35.0	37.0	32.0	37.0
7	35.600245300039724	37.0	35.0	37.0	35.0	37.0
8	35.523781570921116	37.0	35.0	37.0	35.0	37.0
9	37.19699710059693	39.0	37.0	39.0	34.0	39.0
10	36.60404415134999	39.0	35.0	39.0	32.0	39.0
11	36.34896111735297	38.0	35.0	39.0	32.0	39.0
12	35.63947492771421	37.0	35.0	39.0	31.0	39.0
13	35.18458639773858	37.0	35.0	39.0	30.0	39.0
14	36.20175585051002	38.0	35.0	40.0	30.0	41.0
15	36.517535254961025	38.0	35.0	40.0	31.0	41.0
16	36.58023778028043	38.0	35.0	40.0	31.0	41.0
17	36.45592637190214	38.0	35.0	40.0	31.0	41.0
18	36.39348203384246	38.0	35.0	40.0	31.0	41.0
19	36.290885148298244	37.0	35.0	40.0	31.0	41.0
20	36.125933825232465	37.0	34.0	40.0	30.0	41.0
21	35.96589226726943	37.0	34.0	40.0	30.0	41.0
22	35.85035147567526	37.0	34.0	40.0	30.0	41.0
23	35.80048236288729	37.0	34.0	40.0	30.0	41.0
24	35.70931325288933	37.0	34.0	40.0	30.0	41.0
25	35.57364625511116	36.0	34.0	40.0	29.0	41.0
26	35.45523063916625	36.0	34.0	40.0	29.0	41.0
27	35.34842370699333	36.0	34.0	40.0	29.0	41.0
28	35.3594294371562	36.0	34.0	40.0	29.0	41.0
29	35.365974683477035	36.0	34.0	40.0	29.0	41.0
30	35.227139909594605	36.0	34.0	40.0	29.0	41.0
31	35.04773320000301	36.0	34.0	40.0	27.0	41.0
32	34.81663301670334	36.0	34.0	40.0	26.0	41.0
33	34.55771680976205	36.0	33.0	40.0	25.0	41.0
34	34.37802423417363	36.0	33.0	40.0	24.0	41.0
35	34.184623155101455	36.0	33.0	40.0	23.0	41.0
36	33.99853590552206	36.0	33.0	40.0	23.0	41.0
37	33.93052592700519	36.0	33.0	40.0	23.0	41.0
38	33.90059635499818	36.0	33.0	40.0	23.0	41.0
39	33.85435559249991	36.0	33.0	40.0	23.0	41.0
40	33.70861065582664	36.0	33.0	40.0	22.0	41.0
41	33.648290273337494	35.0	33.0	40.0	22.0	41.0
42	33.5970396594231	35.0	33.0	40.0	22.0	41.0
43	33.4804909189385	35.0	32.0	40.0	22.0	41.0
44	33.396772260679676	35.0	32.0	40.0	21.0	41.0
45	33.3586648397224	35.0	32.0	40.0	22.0	41.0
46	33.28149364208337	35.0	32.0	39.0	22.0	41.0
47	33.189780700258765	35.0	32.0	39.0	21.0	41.0
48	33.16070673337316	35.0	32.0	39.0	21.0	41.0
49	33.18574779754753	35.0	32.0	39.0	20.0	41.0
50	33.09090782271968	35.0	32.0	39.0	20.0	41.0
51	32.917360148198604	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	9.0
13	13.0
14	49.0
15	181.0
16	570.0
17	1579.0
18	3478.0
19	6711.0
20	11788.0
21	18630.0
22	27514.0
23	39189.0
24	54505.0
25	74526.0
26	94279.0
27	107878.0
28	115869.0
29	127085.0
30	147747.0
31	177515.0
32	218899.0
33	276162.0
34	432880.0
35	573378.0
36	341873.0
37	406579.0
38	539037.0
39	717799.0
40	372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.71205964789989	22.411872008205304	27.57962508375586	16.296443260138943
2	34.171128110038254	25.123059665171425	28.217453901616924	12.488358323173392
3	29.8910653479483	24.95975511624848	32.56560635698662	12.583573178816598
4	27.10547724564237	26.73929862523034	31.292295878171046	14.862928250956244
5	23.340991855365534	31.48852705275479	30.53755207477599	14.632929017103688
6	19.872358950262857	40.981868877186564	29.056916783544747	10.088855389005829
7	82.89914621060375	5.346823433128836	9.886047746485797	1.8679826097816215
8	83.6764094345079	4.502001947697373	9.88562702968179	1.9359615881129346
9	78.33642818519156	6.700800823364929	11.657951924026516	3.304819067416989
10	45.22769857722434	25.096000931777006	17.563907989677823	12.112392501320828
11	40.216651439670756	22.581620167126427	23.246862006216865	13.954866386985945
12	36.47561547546977	21.2311192262708	27.68092926156229	14.612336036697135
13	21.6964098685105	35.32692574259838	27.31645565135597	15.660208737535159
14	16.74506023114624	37.942300683199804	29.801098380860306	15.511540704793648
15	16.36796511681977	23.09538181378543	44.70142614139362	15.835226928001184
16	18.108669821895077	19.121069453258585	43.61356320118544	19.156697523660892
17	17.49340471052248	19.791182750079603	31.49390779924811	31.221504740149804
18	21.527038140414014	23.05136155029271	35.54133631171307	19.880263997580215
19	28.92764600976683	24.357332938892878	27.30237270991665	19.412648341423644
20	29.92737985103082	22.910111419095493	27.626280363021028	19.536228366852654
21	22.838390275507507	26.990023697427564	29.973636556481676	20.19794947058326
22	22.103907307673744	24.36705371136436	27.738235318865694	25.790803662096206
23	19.669662022691252	28.397852838576277	27.7305295584555	24.201955580276973
24	20.080547339276215	24.263911665414113	38.06477355914459	17.590767436165084
25	19.455960029246462	24.624997398198712	35.463370845034056	20.45567172752077
26	17.391834816839832	31.528827293980516	29.441429799415513	21.63790808976414
27	18.008317792645073	31.51704722346838	30.632456928563617	19.842178055322933
28	16.85728090286712	28.13198196143488	37.405045324485585	17.605691811212413
29	17.829712437850166	24.945583602850423	36.479003352891496	20.745700606407915
30	19.119519443980675	29.31897906646041	33.421056477466635	18.140445012092286
31	27.6787813915629	26.907718204770397	26.729754996676334	18.683745406990365
32	28.167322172971293	26.30990176926916	27.854176012853564	17.668600044905983
33	26.02737936388505	27.745343218554407	25.994541310182985	20.23273610737756
34	19.65878981475618	28.282775721186105	29.433414037149735	22.625020426907984
35	20.925036679862412	25.604935406684792	31.627585027973236	21.842442885479556
36	27.65008407693183	27.6943257703214	26.401440888624748	18.254149264122024
37	20.394069930218585	32.78143407744112	28.474069009070213	18.350426983270086
38	21.72953578107846	32.731723065599496	24.72561514332493	20.81312600999712
39	20.89523221574712	30.715648140808156	27.15574183222611	21.233377811218613
40	23.453677529869786	26.736087891726097	27.536114109025885	22.27412046937824
41	18.079263931594106	25.89618215000458	28.7824322834161	27.242121634985217
42	21.21094696266825	27.19256562407138	25.60075038163443	25.99573703162595
43	21.754247357566324	26.405736628623533	27.07702350389783	24.76299250991231
44	19.661048399703994	29.420039671380323	29.414658924886993	21.504253004028694
45	18.319692513588045	34.68090401855405	25.778248586945114	21.22115488091279
46	21.39794451055357	31.96409204220808	27.471788723992507	19.166174723245845
47	21.46804921589459	28.771626504450076	27.11012727347611	22.650197006179223
48	21.586868498541442	26.49955647591662	30.74957120100476	21.16400382453718
49	20.690431704155486	26.151003675293428	32.361536564054575	20.79702805649651
50	20.12731333348981	31.265502860652838	28.56985958244522	20.03732422341214
51	18.955107745573503	32.604888020686865	26.401108743779485	22.038895489960147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6290.0
1	7892.0
2	9494.0
3	37491.5
4	65489.0
5	48096.5
6	30704.0
7	31508.0
8	32312.0
9	32841.0
10	33370.0
11	33262.0
12	33154.0
13	32274.5
14	31395.0
15	29025.0
16	26655.0
17	25150.0
18	23645.0
19	23137.0
20	22629.0
21	22910.5
22	23192.0
23	25372.5
24	27553.0
25	28975.5
26	33647.5
27	36897.0
28	48347.0
29	59797.0
30	70482.0
31	81167.0
32	90079.0
33	98991.0
34	110416.0
35	121841.0
36	127533.5
37	133226.0
38	155914.0
39	178602.0
40	223270.5
41	267939.0
42	326144.5
43	384350.0
44	392282.5
45	400215.0
46	396486.0
47	392757.0
48	367141.5
49	341526.0
50	325284.0
51	309042.0
52	280160.0
53	251278.0
54	237074.5
55	222871.0
56	212974.5
57	203078.0
58	194488.5
59	185899.0
60	168787.0
61	151675.0
62	129228.5
63	106782.0
64	89420.5
65	72059.0
66	58508.0
67	44957.0
68	35435.5
69	25914.0
70	22356.0
71	18798.0
72	16452.0
73	14106.0
74	10906.0
75	5760.5
76	3815.0
77	3002.0
78	2189.0
79	1659.5
80	1130.0
81	971.5
82	813.0
83	524.0
84	235.0
85	164.0
86	93.0
87	66.0
88	39.0
89	27.0
90	15.0
91	15.5
92	16.0
93	10.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4516102.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.462426795521008
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53786588756061	13.423111482369759
2	6.935236843546271	2.283416576929613
3	2.462621060023337	1.2162215677722756
4	1.219127156494407	0.8027916627288343
5	0.8446253954301705	0.6952291870953582
6	0.5759457340324114	0.568887869082071
7	0.4253772826374771	0.490191966312798
8	0.3253837780542796	0.42852853013349057
9	0.2693057787667714	0.39900840017028755
>10	2.9431338413916075	11.303702925716347
>50	0.944931657976441	11.245142469378088
>100	1.4836845567081494	51.29125208910628
>500	0.025556320112446008	2.77611912065373
>1k	0.006796893646927129	1.8946231982780295
>5k	1.359378729385426E-4	0.13568189330702904
>10k+	2.718757458770852E-4	1.0460910609660354
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	36575	0.8098798477093742	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	10170	0.22519420509102764	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	6063	0.13425294645692237	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	4888	0.10823493357767384	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4534	0.10039631522937259	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016142239479976315	0.0
2	0.0	0.0	0.0	0.05427246771662819	0.0
3	0.0	0.0	0.0	0.08064476843082818	0.0
4	2.2142989684466824E-5	0.0	0.0	0.1164721257402955	0.0
5	2.2142989684466824E-5	0.0	0.0	0.19773689788228876	0.0
6	2.2142989684466824E-5	0.0	0.0	0.31761904403399216	0.0
7	2.2142989684466824E-5	0.0	0.0	0.4057702859678546	0.0
8	2.2142989684466824E-5	0.0	0.0	0.6532624816711403	0.0
9	2.2142989684466824E-5	0.0	0.0	0.7868289954478441	0.0
10	2.2142989684466824E-5	0.0	0.0	0.9864923334326815	0.0
11	2.2142989684466824E-5	0.0	0.0	1.1121095139126618	0.0
12	2.2142989684466824E-5	0.0	0.0	1.2373724065576908	0.0
13	2.2142989684466824E-5	0.0	0.0	1.2926191658204353	0.0
14	2.2142989684466824E-5	0.0	0.0	1.3143414387008974	0.0
15	2.2142989684466824E-5	0.0	0.0	1.3426180365279614	0.0
16	2.2142989684466824E-5	0.0	0.0	1.4074527103240804	0.0
17	2.2142989684466824E-5	0.0	0.0	1.477778845561947	0.0
18	2.2142989684466824E-5	0.0	0.0	1.6029310232585534	0.0
19	2.2142989684466824E-5	0.0	0.0	1.6472391456171716	0.0
20	4.428597936893365E-5	0.0	0.0	1.7050987776626834	0.0
21	6.642896905340048E-5	0.0	0.0	1.765748426408438	0.0
22	6.642896905340048E-5	0.0	0.0	1.827460938659047	0.0
23	6.642896905340048E-5	0.0	0.0	1.9019056699782246	0.0
24	6.642896905340048E-5	0.0	0.0	1.9621346019199744	0.0
25	6.642896905340048E-5	0.0	0.0	2.0110484661329617	0.0
26	6.642896905340048E-5	0.0	0.0	2.0609809078714343	0.0
27	6.642896905340048E-5	0.0	0.0	2.11410194012447	0.0
28	8.85719587378673E-5	0.0	0.0	2.1706551357785986	0.0
29	8.85719587378673E-5	0.0	0.0	2.232168361122047	0.0
30	8.85719587378673E-5	0.0	0.0	2.3117724090377054	0.0
31	8.85719587378673E-5	0.0	0.0	2.3820321153065187	0.0
32	8.85719587378673E-5	0.0	0.0	2.449789663740987	0.0
33	8.85719587378673E-5	0.0	0.0	2.522307954957616	0.0
34	8.85719587378673E-5	0.0	0.0	2.59752769091575	0.0
35	8.85719587378673E-5	0.0	0.0	2.6851696440868698	0.0
36	8.85719587378673E-5	0.0	0.0	2.7610093837561687	0.0
37	8.85719587378673E-5	0.0	0.0	2.842384870846584	0.0
38	8.85719587378673E-5	0.0	0.0	2.9257089410292325	0.0
39	8.85719587378673E-5	0.0	0.0	3.0100515887373667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	27485	0.0	43.16627	1
CGCATCG	335	0.0	42.985077	21
CGACGGT	840	0.0	42.857143	28
TAATGCG	275	0.0	42.545456	1
GCGTAAG	455	0.0	41.53846	1
CGTAAGG	765	0.0	41.47059	2
TAGCCGT	475	0.0	40.263157	44
CGTATGA	185	0.0	40.135136	15
CGGTCTA	910	0.0	39.80769	31
TAGCATA	4400	0.0	39.528408	30
CGTCGAA	40	3.4612276E-7	39.375	14
TATAGCG	260	0.0	38.94231	1
GTTGATC	4630	0.0	38.633907	16
CATATGC	4505	0.0	38.6071	33
ACGTAAG	140	0.0	38.57143	1
GTAGCAT	4595	0.0	38.43852	29
GGTACCT	4745	0.0	38.40885	8
ACCCGCT	2725	0.0	38.394497	34
ACGGTCT	945	0.0	38.333332	30
ACCTGGT	4715	0.0	38.271477	11
>>END_MODULE