FastQCFastQC Report
Sat 18 Jun 2016
SRR3548907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393238
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70290.50450820319285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46210.3316734111472699No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT15280.109672575683408Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT15060.10809352027435369Illumina Single End Adapter 2 (95% over 23bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG351.01978E-746.0000041
CTTAACG700.046.0000041
CGAACTG351.01978E-746.00000414
GTTTCGA351.01978E-746.00000438
TATATCG351.01978E-746.0000041
CTACGGC351.01978E-746.00000416
GGAACGA700.046.0000048
CTCGCTA351.01978E-746.00000412
GAACGAT351.01978E-746.0000049
AACGTCG405.6134013E-946.01
GTACCGC405.6134013E-946.031
ACCGGTC206.311918E-446.024
GTCGATG600.046.01
CTCGTAT600.046.011
GTCGAAT206.311918E-446.029
GTCGAAA501.6370905E-1146.012
CGACGTG206.311918E-446.044
CGACGGT405.6134013E-946.028
GCGAATT206.311918E-446.023
CGACGCC253.4175326E-546.04
Produced by FastQC (version 0.11.5)