##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548907_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1393238
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71770580475123	33.0	31.0	34.0	31.0	34.0
2	33.13082832940244	34.0	33.0	34.0	31.0	34.0
3	33.16284583107839	34.0	33.0	34.0	31.0	34.0
4	36.48375941511787	37.0	37.0	37.0	35.0	37.0
5	36.47811500978297	37.0	37.0	37.0	35.0	37.0
6	36.552375114660954	37.0	37.0	37.0	35.0	37.0
7	36.66263265859817	37.0	37.0	37.0	35.0	37.0
8	36.66800001148404	37.0	37.0	37.0	35.0	37.0
9	38.43756845564074	39.0	39.0	39.0	37.0	39.0
10	38.296911941821854	39.0	39.0	39.0	37.0	39.0
11	38.06972247383433	39.0	39.0	39.0	35.0	39.0
12	37.51096869307326	39.0	35.0	39.0	35.0	39.0
13	37.436861469468965	39.0	35.0	39.0	35.0	39.0
14	38.69240431283097	41.0	36.0	41.0	35.0	41.0
15	38.70528079193935	41.0	36.0	41.0	35.0	41.0
16	38.78329330667122	41.0	36.0	41.0	35.0	41.0
17	38.74276326083555	41.0	36.0	41.0	35.0	41.0
18	38.751144456295336	40.0	36.0	41.0	35.0	41.0
19	38.74489498563777	40.0	36.0	41.0	35.0	41.0
20	38.610580532543615	40.0	36.0	41.0	35.0	41.0
21	38.55771303969602	40.0	35.0	41.0	35.0	41.0
22	38.4739283596916	40.0	35.0	41.0	35.0	41.0
23	38.399968993093786	40.0	35.0	41.0	35.0	41.0
24	38.34420608682795	40.0	35.0	41.0	35.0	41.0
25	38.36539270390271	40.0	35.0	41.0	35.0	41.0
26	38.31053057697249	40.0	35.0	41.0	35.0	41.0
27	38.28084433528227	40.0	35.0	41.0	35.0	41.0
28	38.263336199558154	40.0	35.0	41.0	35.0	41.0
29	38.244648078792	40.0	36.0	41.0	35.0	41.0
30	38.19900189343099	40.0	36.0	41.0	35.0	41.0
31	38.101741411015205	40.0	35.0	41.0	35.0	41.0
32	37.97994168979026	40.0	35.0	41.0	35.0	41.0
33	37.855943492784434	40.0	35.0	41.0	35.0	41.0
34	37.74934433312902	40.0	35.0	41.0	35.0	41.0
35	37.63882839830668	40.0	35.0	41.0	34.0	41.0
36	37.558664061703745	40.0	35.0	41.0	34.0	41.0
37	37.530891348068316	40.0	35.0	41.0	34.0	41.0
38	37.43535347155332	40.0	35.0	41.0	34.0	41.0
39	37.44344900153455	40.0	35.0	41.0	34.0	41.0
40	37.36647148584807	40.0	35.0	41.0	34.0	41.0
41	37.31776695726071	40.0	35.0	41.0	34.0	41.0
42	37.29890872916185	40.0	35.0	41.0	34.0	41.0
43	37.25693671863673	40.0	35.0	41.0	34.0	41.0
44	37.16232617829832	40.0	35.0	41.0	34.0	41.0
45	37.09244077465588	40.0	35.0	41.0	34.0	41.0
46	37.057747491814034	40.0	35.0	41.0	34.0	41.0
47	36.99851497016303	39.0	35.0	41.0	33.0	41.0
48	36.95030066650493	39.0	35.0	41.0	34.0	41.0
49	36.903018723290636	39.0	35.0	41.0	33.0	41.0
50	36.79684590859566	39.0	35.0	41.0	33.0	41.0
51	36.75444683535763	39.0	35.0	41.0	33.0	41.0
52	36.55153319102695	39.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	4.0
13	3.0
14	7.0
15	11.0
16	28.0
17	62.0
18	134.0
19	284.0
20	478.0
21	759.0
22	1388.0
23	2405.0
24	4307.0
25	6947.0
26	9564.0
27	10193.0
28	9836.0
29	10003.0
30	10997.0
31	13161.0
32	16823.0
33	23676.0
34	59966.0
35	254187.0
36	55572.0
37	66659.0
38	132123.0
39	701297.0
40	2358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.792889657043524	23.754089394633223	30.38368175430185	13.06933919402141
2	29.273534026490804	26.647923757462834	30.954366734183246	13.124175481863112
3	27.077426828725603	25.924429279132493	33.281463755654094	13.716680136487808
4	24.971038688293028	28.108047584117003	32.371497188563616	14.549416539026355
5	22.297051903551296	34.9922267408727	28.414312558227667	14.296408797348336
6	21.739932445138592	43.62887030069522	24.72391651677603	9.907280737390165
7	90.58229821466254	3.131553977138149	5.016156607844461	1.2699912003548568
8	91.18341589879117	2.496271275977256	4.785255641893201	1.5350571833383815
9	87.11024247113558	4.8799271911905935	5.787238074184023	2.2225922634897985
10	44.43576761472197	37.10227541884445	10.889596752313675	7.572360214119914
11	34.3540012546313	21.349977534348046	30.37980589102508	13.916215319995578
12	36.12606029981956	21.32385134485278	28.247506886834838	14.30258146849282
13	19.51274656591336	37.07657988082438	28.308659396312763	15.102014156949494
14	13.563081110334343	41.04467434853198	29.511110090307614	15.881134450826062
15	12.536264442973849	28.190876217846483	45.17010015517808	14.102759184001584
16	15.768088438586947	24.08116919004506	43.17639915075529	16.974343220612703
17	15.316909243072613	24.039754873180318	30.840746519977202	29.802589363769865
18	17.135693973319704	27.04462554136479	37.30776794775911	18.511912537556398
19	23.873308078016823	26.315891470086232	29.435746082148206	20.375054369748742
20	26.1829637147422	26.647923757462834	31.3991579328155	15.769954594979465
21	20.471376749701058	29.03897252300038	32.405375104612425	18.084275622686146
22	20.86506397327664	25.346782100402088	28.57638106339333	25.211772862927944
23	16.63039624242233	30.929963150588772	29.176278568342234	23.26336203864666
24	15.775481288911156	25.822580205248492	41.885736679591	16.516201826249354
25	14.939299674571036	29.996597853345946	36.73169982443775	18.332402647645267
26	15.706146401404498	35.426754079346104	29.718971202335855	19.14812831691355
27	15.152472154793367	38.24852609532614	29.829720406707256	16.769281343173244
28	13.44594390908086	30.983651034496617	38.18565097994743	17.38475407647509
29	14.621550661121791	25.56720388045689	36.62324742793406	23.187998030487254
30	16.240728432615246	34.83776641176885	31.114999734431592	17.806505421184323
31	24.412340174471268	30.247811213877313	28.271336268462388	17.06851234318903
32	25.25735014405292	27.763311078222095	29.291406062711467	17.687932715013517
33	24.4552617714992	30.71133575168062	27.244878477331223	17.588523999488963
34	18.035683781234795	29.585541020270767	28.692728736942286	23.686046461552156
35	18.9887872710908	27.284785514032777	32.80107203507225	20.925355179804168
36	26.06848219758577	28.869152291281175	27.542817522921425	17.519547988211635
37	17.320371680933196	34.98433146382743	31.52770739816169	16.167589457077685
38	17.417124712360703	32.90844780288795	28.901810028150248	20.772617456601097
39	18.097410492679643	33.90160187993724	30.671213389241466	17.329774238141653
40	22.80242140969454	28.797951247382	28.004834780561538	20.39479256236192
41	16.23304848130757	28.231357456514967	31.297165308439762	24.238428753737693
42	18.92720411013768	26.558420025867797	29.336983343836444	25.177392520158076
43	19.568731257688924	26.67814113597246	30.334013284162502	23.41911432217611
44	16.313149655694144	30.169217319653928	31.84043214440031	21.677200880251615
45	15.254464779169103	39.53509737747606	26.079966236924346	19.13047160643049
46	19.52257977459702	35.732157750506374	27.02589220219374	17.719370272702868
47	17.14832641659214	30.578551546828326	30.391074604626056	21.882047431953477
48	19.35764025959671	27.105706275596848	34.568752790262685	18.96790067454376
49	20.36019689385446	27.480229508526183	33.291942941550545	18.867630656068812
50	18.531866055907177	35.29741508629538	28.59131031453348	17.579408543263966
51	16.51175176100566	33.57638824091792	27.624497752717055	22.287362245359372
52	18.023266663699957	29.23527782044417	34.869347519949926	17.87210799590594
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5517.0
1	4805.5
2	4094.0
3	7778.0
4	11462.0
5	7513.5
6	3565.0
7	3841.5
8	4118.0
9	4646.0
10	5174.0
11	5745.0
12	6316.0
13	6281.0
14	5913.0
15	5580.0
16	5267.5
17	4955.0
18	5170.0
19	5385.0
20	7105.5
21	8826.0
22	9019.0
23	9212.0
24	11388.0
25	13564.0
26	17061.5
27	20559.0
28	24224.0
29	27889.0
30	34770.0
31	41651.0
32	45190.5
33	48730.0
34	57461.0
35	66192.0
36	68136.0
37	70080.0
38	75456.0
39	88851.5
40	96871.0
41	117775.5
42	138680.0
43	144805.0
44	150930.0
45	140369.5
46	129809.0
47	117973.5
48	106138.0
49	99492.0
50	92846.0
51	84906.0
52	76966.0
53	65003.5
54	53041.0
55	43539.5
56	34038.0
57	27727.5
58	21417.0
59	18541.0
60	15665.0
61	13648.5
62	11632.0
63	9072.5
64	5343.0
65	4173.0
66	2879.5
67	1586.0
68	1258.5
69	931.0
70	836.5
71	742.0
72	689.0
73	636.0
74	487.0
75	338.0
76	240.0
77	142.0
78	135.0
79	128.0
80	72.5
81	17.0
82	21.5
83	26.0
84	13.5
85	1.0
86	11.5
87	22.0
88	11.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1393238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.86221523625132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61025131154855	11.237358290592882
2	9.41769927438864	2.7993574729250366
3	3.2267969484530994	1.4387205231476674
4	1.7117337249747608	1.0176062019090044
5	1.0306543909570678	0.7658903696295728
6	0.7685997805128516	0.6853857221110513
7	0.5787878101290556	0.6021448307179609
8	0.45546766584467796	0.5415406786349277
9	0.36710898175513085	0.4910447431805232
>10	3.8423571725572896	13.384808614147897
>50	1.1618286031842164	12.386505977869927
>100	1.8146286210239324	52.208313831189734
>500	0.010685714577764056	1.098411155618122
>1k	0.0029142857939356516	0.8355028072505352
>5k	4.857142989892752E-4	0.5074087810751695
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7029	0.50450820319285	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4621	0.3316734111472699	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1528	0.109672575683408	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	1506	0.10809352027435369	Illumina Single End Adapter 2 (95% over 23bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1111798558465962	0.0
2	0.0	0.0	0.0	0.3595222065433185	0.0
3	0.0	0.0	0.0	0.4974742290979718	0.0
4	0.0	0.0	0.0	0.6680122132758366	0.0
5	0.0	0.0	0.0	1.1326851550129986	0.0
6	0.0	0.0	0.0	1.4230877997872582	0.0
7	0.0	0.0	0.0	1.6373369086975806	0.0
8	0.0	0.0	0.0	2.132370779436105	0.0
9	0.0	0.0	0.0	2.303124089351568	0.0
10	7.177524586610472E-5	0.0	0.0	2.6954475832556963	0.0
11	7.177524586610472E-5	0.0	0.0	3.1744755741660793	0.0
12	7.177524586610472E-5	0.0	0.0	3.5383760707072303	0.0
13	1.4355049173220944E-4	0.0	0.0	3.6634803242518506	0.0
14	1.4355049173220944E-4	0.0	0.0	3.7113544132445426	0.0
15	1.4355049173220944E-4	0.0	0.0	3.7898047569761952	0.0
16	1.4355049173220944E-4	0.0	0.0	3.970965477542243	0.0
17	1.4355049173220944E-4	0.0	0.0	4.202871296935627	0.0
18	1.4355049173220944E-4	0.0	0.0	4.461190406807739	0.0
19	1.4355049173220944E-4	0.0	0.0	4.629431583117888	0.0
20	1.4355049173220944E-4	0.0	0.0	4.77865231927352	0.0
21	2.1532573759831415E-4	0.0	0.0	4.973450336554128	0.0
22	2.1532573759831415E-4	0.0	0.0	5.190426904807363	0.0
23	2.1532573759831415E-4	0.0	0.0	5.430084450754286	0.0
24	3.588762293305236E-4	0.0	0.0	5.612393575254192	0.0
25	3.588762293305236E-4	0.0	0.0	5.760465907475966	0.0
26	3.588762293305236E-4	0.0	0.0	5.905093027896167	0.0
27	3.588762293305236E-4	0.0	0.0	6.045987835531331	0.0
28	4.306514751966283E-4	0.0	0.0	6.195208571686963	0.0
29	4.306514751966283E-4	0.0	0.0	6.364813477668568	0.0
30	4.306514751966283E-4	0.0	0.0	6.56542528986433	0.0
31	4.306514751966283E-4	0.0	0.0	6.752902232066596	0.0
32	5.02426721062733E-4	0.0	0.0	6.916549792641315	0.0
33	5.02426721062733E-4	0.0	0.0	7.08615469862292	0.0
34	5.02426721062733E-4	0.0	0.0	7.265233937058851	0.0
35	5.02426721062733E-4	0.0	0.0	7.483000033016613	0.0
36	5.02426721062733E-4	0.0	0.0	7.68519090062143	0.0
37	5.02426721062733E-4	0.0	0.0	7.875036425937277	0.0
38	5.02426721062733E-4	0.0	0.0	8.066891658137376	0.0
39	5.02426721062733E-4	0.0	0.0	8.258029137878811	0.0
40	5.02426721062733E-4	0.0	0.0	8.464957171710791	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	35	1.01978E-7	46.000004	1
CTTAACG	70	0.0	46.000004	1
CGAACTG	35	1.01978E-7	46.000004	14
GTTTCGA	35	1.01978E-7	46.000004	38
TATATCG	35	1.01978E-7	46.000004	1
CTACGGC	35	1.01978E-7	46.000004	16
GGAACGA	70	0.0	46.000004	8
CTCGCTA	35	1.01978E-7	46.000004	12
GAACGAT	35	1.01978E-7	46.000004	9
AACGTCG	40	5.6134013E-9	46.0	1
GTACCGC	40	5.6134013E-9	46.0	31
ACCGGTC	20	6.311918E-4	46.0	24
GTCGATG	60	0.0	46.0	1
CTCGTAT	60	0.0	46.0	11
GTCGAAT	20	6.311918E-4	46.0	29
GTCGAAA	50	1.6370905E-11	46.0	12
CGACGTG	20	6.311918E-4	46.0	44
CGACGGT	40	5.6134013E-9	46.0	28
GCGAATT	20	6.311918E-4	46.0	23
CGACGCC	25	3.4175326E-5	46.0	4
>>END_MODULE