##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548906_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1685649
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.688325980082446	33.0	31.0	34.0	31.0	34.0
2	33.10729873182377	34.0	33.0	34.0	31.0	34.0
3	33.15747228515545	34.0	33.0	34.0	31.0	34.0
4	36.46546997625247	37.0	37.0	37.0	35.0	37.0
5	36.460798185150054	37.0	37.0	37.0	35.0	37.0
6	36.52187080465743	37.0	37.0	37.0	35.0	37.0
7	36.62532472655933	37.0	37.0	37.0	35.0	37.0
8	36.627243275438715	37.0	37.0	37.0	35.0	37.0
9	38.35411642637346	39.0	39.0	39.0	37.0	39.0
10	38.25308590341168	39.0	39.0	39.0	37.0	39.0
11	38.10678083041012	39.0	39.0	39.0	35.0	39.0
12	37.44626491042916	39.0	35.0	39.0	35.0	39.0
13	37.323842033543166	39.0	35.0	39.0	35.0	39.0
14	38.515368857929495	40.0	35.0	41.0	35.0	41.0
15	38.54316586667806	40.0	35.0	41.0	35.0	41.0
16	38.61460304013469	40.0	35.0	41.0	35.0	41.0
17	38.56292027581068	40.0	35.0	41.0	35.0	41.0
18	38.60952487736178	40.0	36.0	41.0	35.0	41.0
19	38.593410015964174	40.0	36.0	41.0	35.0	41.0
20	38.451642661075944	40.0	35.0	41.0	35.0	41.0
21	38.3815509634568	40.0	35.0	41.0	35.0	41.0
22	38.339010671853984	40.0	35.0	41.0	35.0	41.0
23	38.270934221774525	40.0	35.0	41.0	35.0	41.0
24	38.22299185654902	40.0	35.0	41.0	35.0	41.0
25	38.23089741695929	40.0	35.0	41.0	35.0	41.0
26	38.1689343392367	40.0	35.0	41.0	35.0	41.0
27	38.147833267779944	40.0	35.0	41.0	35.0	41.0
28	38.131647810427914	40.0	35.0	41.0	35.0	41.0
29	38.098951798387446	40.0	35.0	41.0	35.0	41.0
30	38.05579453373745	40.0	36.0	41.0	35.0	41.0
31	37.968887947609495	40.0	35.0	41.0	35.0	41.0
32	37.847341291099156	40.0	35.0	41.0	35.0	41.0
33	37.75533577868227	40.0	35.0	41.0	35.0	41.0
34	37.624134087226935	40.0	35.0	41.0	35.0	41.0
35	37.50770059484507	40.0	35.0	41.0	34.0	41.0
36	37.45795476994321	40.0	35.0	41.0	34.0	41.0
37	37.420496793816504	40.0	35.0	41.0	34.0	41.0
38	37.311900638863726	40.0	35.0	41.0	34.0	41.0
39	37.326513408188774	40.0	35.0	41.0	34.0	41.0
40	37.24997078276676	40.0	35.0	41.0	33.0	41.0
41	37.22546330819761	40.0	35.0	41.0	34.0	41.0
42	37.206995050571024	40.0	35.0	41.0	34.0	41.0
43	37.16396058728715	40.0	35.0	41.0	34.0	41.0
44	37.06913716912596	39.0	35.0	41.0	34.0	41.0
45	37.002361701635394	39.0	35.0	41.0	34.0	41.0
46	36.943910624335196	39.0	35.0	41.0	34.0	41.0
47	36.89776163364971	39.0	35.0	41.0	33.0	41.0
48	36.84574309360964	39.0	35.0	41.0	33.0	41.0
49	36.83726030745428	39.0	35.0	41.0	33.0	41.0
50	36.71371501421707	39.0	35.0	41.0	33.0	41.0
51	36.64959964974915	39.0	35.0	41.0	33.0	41.0
52	36.440296289441044	38.0	35.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	3.0
11	3.0
12	4.0
13	2.0
14	9.0
15	14.0
16	43.0
17	78.0
18	186.0
19	403.0
20	657.0
21	1168.0
22	1850.0
23	2973.0
24	5056.0
25	7992.0
26	11019.0
27	12369.0
28	12652.0
29	13306.0
30	14885.0
31	17481.0
32	22384.0
33	30532.0
34	75646.0
35	328036.0
36	66197.0
37	80847.0
38	162590.0
39	814203.0
40	3058.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.401139857704656	24.70781283648019	29.536160849619346	14.354886456195803
2	29.292041225664416	26.20426909754047	31.094195766734355	13.409493910060755
3	28.00559309796998	25.284919932916043	33.0860101954796	13.62347677363437
4	24.866802044791054	27.959142146437365	31.68281178347331	15.491244025298268
5	22.262701191054603	34.17200140717314	29.360442179836966	14.20485522193529
6	21.67438179597295	41.40310349307596	26.265610456269368	10.656904254681727
7	88.87449285112143	3.698575444828668	5.794741372610787	1.6321903314391075
8	89.72959376477546	2.7795822261929977	5.6952544687535775	1.7955695402779581
9	84.7601131670947	5.67864365594498	6.983541650723253	2.5777015262370755
10	42.080409385346535	35.69450104974404	12.60155583991685	9.62353372499257
11	41.87283354957052	19.951959156384277	23.71828298773944	14.45692430630576
12	39.54352299915344	20.834467911172492	24.764408248692344	14.857600840981721
13	21.506019343291516	38.202259189190634	25.129668157487117	15.162053310030736
14	14.441796601783647	40.19947213209868	28.6678306100499	16.690900656067782
15	13.974676815873293	25.407424677379453	45.384715323296845	15.23318318345041
16	16.843957431232717	22.13408604045089	44.05674016358091	16.96521636473548
17	15.836333661396887	20.89960602711478	30.774496944500306	32.48956336698803
18	19.138207301757365	23.8743653038088	37.631381147558	19.35604624687583
19	25.79149039924682	25.035639092124164	28.559029786153584	20.613840722475437
20	27.775533340570902	24.656022695116242	29.692421138683084	17.87602282562977
21	20.22473243243404	28.60631127832663	32.14340589292314	19.025550396316195
22	21.096859429216877	24.1288666857691	27.187391918483623	27.586881966530395
23	16.90209527606281	30.170219304256108	27.914233627522695	25.013451792158392
24	16.49863049780826	23.545471210198563	42.217329942354546	17.738568349638626
25	15.319678058718036	27.266886522639055	36.88644551742385	20.526989901219057
26	16.12364139865417	33.71063608141434	29.034336329805317	21.13138619012618
27	15.51093970334275	36.17627394552484	29.59234099151128	18.72044535962113
28	14.290756853888325	31.724872734477938	36.48754871269167	17.49682169894207
29	15.075439786100189	24.60541904038148	36.370501806722515	23.948639366795817
30	16.497325362516158	33.64721837108437	30.555471512752653	19.299984753646815
31	25.196823300699016	29.694734787609995	27.530345878649708	17.57809603304128
32	25.275250066888184	26.035076104218614	30.592549219914705	18.0971246089785
33	26.184336122170155	27.852714295799423	26.38188614592955	19.581063436100873
34	18.626950213241308	27.913759032871017	29.061922143933877	24.397368609953794
35	17.503762645722805	26.21275247693915	33.34092684776012	22.942558029577924
36	27.160043401680895	27.094727312744233	27.71763279306665	18.027596492508227
37	17.97812000007119	34.432850492599584	30.973055481894512	16.615974025434713
38	17.77428159717711	33.71324635199855	26.736704972387486	21.77576707843685
39	18.546625068445447	32.42430660238282	29.577865854635217	19.451202474536515
40	22.218267266791603	26.395352769170806	29.26006541100787	22.126314553029726
41	16.161727619451025	25.98435380082093	30.734986939748428	27.118931639979614
42	18.236062193256128	26.133198548452256	28.335733002540863	27.295006255750753
43	18.8970538943754	25.40368724449752	30.430534470699417	25.268724390427664
44	16.6449836235183	29.39532488673502	31.915837757445352	22.043853732301326
45	16.30725020452063	37.13691284484492	25.770192964252935	20.785643986381505
46	19.77190981040537	33.74866297787973	27.643892649062764	18.83553456265213
47	18.50877614497443	29.400842049560733	28.449635718942673	23.640746086522167
48	18.668595893925723	27.174518538556956	33.578105524934315	20.578780042583006
49	20.45781773073754	26.670083748158717	33.42884550698277	19.44325301412097
50	19.013210935372665	33.747061220930334	28.44340666413945	18.796321179557548
51	17.73649199803755	32.38236430004111	27.611560888417458	22.26958281350388
52	18.61045804909563	27.474047088094856	34.31776128956859	19.59773357324093
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4852.0
1	5120.0
2	5388.0
3	9891.0
4	14394.0
5	9491.5
6	4589.0
7	4507.5
8	4426.0
9	4622.0
10	4818.0
11	4883.0
12	4948.0
13	4923.0
14	5185.5
15	5473.0
16	5359.5
17	5246.0
18	6028.0
19	6810.0
20	6122.5
21	5435.0
22	7381.0
23	9327.0
24	10765.0
25	12203.0
26	14807.5
27	17412.0
28	21037.5
29	24663.0
30	30039.0
31	35415.0
32	41150.0
33	46885.0
34	53343.5
35	59802.0
36	68663.5
37	77525.0
38	80339.5
39	100581.0
40	118008.0
41	145010.5
42	172013.0
43	180212.5
44	188412.0
45	186461.0
46	184510.0
47	164674.0
48	144838.0
49	127714.5
50	110591.0
51	97133.0
52	83675.0
53	73661.0
54	63647.0
55	55180.5
56	46714.0
57	41788.5
58	36863.0
59	33956.0
60	31049.0
61	27698.0
62	24347.0
63	19603.5
64	12480.5
65	10101.0
66	8366.5
67	6632.0
68	5268.0
69	3904.0
70	3573.0
71	3242.0
72	2470.0
73	1698.0
74	1413.5
75	1129.0
76	937.0
77	745.0
78	588.0
79	431.0
80	362.5
81	294.0
82	199.0
83	104.0
84	90.0
85	76.0
86	74.5
87	73.0
88	46.0
89	13.5
90	8.0
91	5.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1685649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.248453646815543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.56464661100817	11.369955575391398
2	10.32842025606961	3.149848750390163
3	3.685791531365253	1.68607863953545
4	1.7687965516338797	1.0788564891294556
5	1.0279477214944388	0.7837306591278802
6	0.7187098650915743	0.6575528438014755
7	0.5247134444146534	0.5600748044512435
8	0.4055271844298954	0.49469299794423016
9	0.3259861848365543	0.44737067060841973
>10	3.929049510678699	14.173425250937191
>50	1.1107073563577463	11.952455859638002
>100	1.5905266648591636	50.857271909729896
>500	0.015654790008456335	1.4572133737759985
>1k	0.0031309580016912666	0.8216444315729358
>5k	3.913697502114083E-4	0.509827743966269
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8543	0.5068077636566094	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3962	0.23504300124165828	No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	1851	0.10980933753112303	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10286839075038753	0.0
2	0.0	0.0	0.0	0.35529342110961415	0.0
3	0.0	0.0	0.0	0.4980870869320956	0.0
4	0.0	0.0	0.0	0.6873910286186508	0.0
5	0.0	0.0	0.0	1.1820373043261083	0.0
6	0.0	0.0	0.0	1.5366188334582112	0.0
7	0.0	0.0	0.0	1.7708906183908988	0.0
8	0.0	0.0	0.0	2.282147706906954	0.0
9	0.0	0.0	0.0	2.4469506997008272	0.0
10	0.0	0.0	0.0	2.793820065743224	0.0
11	0.0	0.0	0.0	3.257795662086235	0.0
12	0.0	0.0	0.0	3.6435817895659177	0.0
13	0.0	0.0	0.0	3.7776547786638854	0.0
14	0.0	0.0	0.0	3.8216734326066697	0.0
15	5.9324331459277705E-5	0.0	0.0	3.908049659211378	0.0
16	5.9324331459277705E-5	0.0	0.0	4.102752115060728	0.0
17	5.9324331459277705E-5	0.0	0.0	4.337617143308008	0.0
18	1.1864866291855541E-4	0.0	0.0	4.652510694693854	0.0
19	1.1864866291855541E-4	0.0	0.0	4.819212066094424	0.0
20	1.1864866291855541E-4	0.0	0.0	5.004185331584452	0.0
21	1.1864866291855541E-4	0.0	0.0	5.244864144314741	0.0
22	1.1864866291855541E-4	0.0	0.0	5.502984310494059	0.0
23	1.1864866291855541E-4	0.0	0.0	5.798241508166884	0.0
24	1.779729943778331E-4	0.0	0.0	6.005758019611437	0.0
25	1.779729943778331E-4	0.0	0.0	6.190138041786873	0.0
26	1.779729943778331E-4	0.0	0.0	6.368407657822002	0.0
27	1.779729943778331E-4	0.0	0.0	6.537304029486566	0.0
28	2.3729732583711082E-4	0.0	0.0	6.720200943375519	0.0
29	2.3729732583711082E-4	0.0	0.0	6.915377993876542	0.0
30	2.3729732583711082E-4	0.0	0.0	7.1653114023144795	0.0
31	2.3729732583711082E-4	0.0	0.0	7.390743861859734	0.0
32	3.559459887556662E-4	0.0	0.0	7.612023618202841	0.0
33	4.1527032021494394E-4	0.0	0.0	7.81657391307443	0.0
34	4.1527032021494394E-4	0.0	0.0	8.035362047496246	0.0
35	4.1527032021494394E-4	0.0	0.0	8.296804376237283	0.0
36	4.1527032021494394E-4	0.0	0.0	8.535703459023795	0.0
37	4.1527032021494394E-4	0.0	0.0	8.757517134350033	0.0
38	4.1527032021494394E-4	0.0	0.0	8.991551621956884	0.0
39	4.1527032021494394E-4	0.0	0.0	9.220899487378452	0.0
40	4.1527032021494394E-4	0.0	0.0	9.491418438832758	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	75	0.0	46.000004	1
ACAACGC	20	6.312298E-4	46.0	36
CGTATCA	30	1.861923E-6	46.0	38
GTCGAAA	25	3.4178393E-5	46.0	13
ACGCATT	25	3.4178393E-5	46.0	24
CGACGAA	20	6.312298E-4	46.0	23
CTATCGG	25	3.4178393E-5	46.0	13
ACCGACA	30	1.861923E-6	46.0	18
ATGTCGC	50	1.6370905E-11	46.0	39
TACGACC	25	3.4178393E-5	46.0	28
CGCTAGC	25	3.4178393E-5	46.0	24
TATTCGA	55	1.8189894E-12	46.0	26
TCGCTAG	30	1.861923E-6	46.0	1
TCGCTAC	20	6.312298E-4	46.0	19
TCGTCTA	20	6.312298E-4	46.0	20
GGACGTA	20	6.312298E-4	46.0	8
ACTCGTC	20	6.312298E-4	46.0	18
TCGTACA	50	1.6370905E-11	46.0	34
CCGGTTA	20	6.312298E-4	46.0	30
ACCGTAT	30	1.861923E-6	46.0	36
>>END_MODULE