FastQCFastQC Report
Sat 18 Jun 2016
SRR3548903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1675550
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86270.5148757124526275No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46490.27746113216555757No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT24500.14622064396765241Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT21850.13040494166094715Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA20000.11936379099400196No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG206.3122855E-446.031
TCGCAAT206.3122855E-446.040
GTCGAAC253.41783E-546.028
ACGCATT206.3122855E-446.043
ACGTTAG301.8619176E-646.01
GACGTAA301.8619176E-646.034
GGCCGTA206.3122855E-446.08
ATAGCCG253.41783E-546.044
AAGCGTA405.6134013E-946.041
TACGCTA405.6134013E-946.013
TACGCCC900.046.038
GTCGTTA301.8619176E-646.08
TGTTACG501.6370905E-1146.01
GTCCGAT301.8619176E-646.021
ACTAACG301.8619176E-646.020
ACGTCGT206.3122855E-446.032
CGTAATG253.41783E-546.021
CGCGTAG206.3122855E-446.046
CGATCTA206.3122855E-446.010
TCGACTA253.41783E-546.045
Produced by FastQC (version 0.11.5)