##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3548903_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1675550 Sequences flagged as poor quality 0 Sequence length 52 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.647116469219064 33.0 31.0 34.0 31.0 34.0 2 33.06688251618871 34.0 33.0 34.0 31.0 34.0 3 33.122640327056786 34.0 33.0 34.0 31.0 34.0 4 36.46121333293545 37.0 37.0 37.0 35.0 37.0 5 36.45665184566262 37.0 37.0 37.0 35.0 37.0 6 36.52107964548954 37.0 37.0 37.0 35.0 37.0 7 36.60466831786577 37.0 37.0 37.0 35.0 37.0 8 36.595717227179136 37.0 37.0 37.0 35.0 37.0 9 38.27752260451792 39.0 39.0 39.0 37.0 39.0 10 38.185636358210736 39.0 39.0 39.0 37.0 39.0 11 38.03691862373549 39.0 39.0 39.0 35.0 39.0 12 37.39841544567455 39.0 35.0 39.0 35.0 39.0 13 37.278004834233535 39.0 35.0 39.0 35.0 39.0 14 38.47458864253529 40.0 35.0 41.0 35.0 41.0 15 38.4944280982364 40.0 35.0 41.0 35.0 41.0 16 38.56465399421086 40.0 35.0 41.0 35.0 41.0 17 38.52137924860494 40.0 35.0 41.0 35.0 41.0 18 38.53481483691922 40.0 36.0 41.0 35.0 41.0 19 38.49450986243323 40.0 36.0 41.0 35.0 41.0 20 38.35396914446003 40.0 35.0 41.0 35.0 41.0 21 38.291123511682734 40.0 35.0 41.0 35.0 41.0 22 38.20721852526036 40.0 35.0 41.0 35.0 41.0 23 38.11136582017845 40.0 35.0 41.0 35.0 41.0 24 38.067887559308886 40.0 35.0 41.0 35.0 41.0 25 38.06395810330936 40.0 35.0 41.0 35.0 41.0 26 38.01181224075677 40.0 35.0 41.0 35.0 41.0 27 37.988743994509264 40.0 35.0 41.0 35.0 41.0 28 37.9434406612754 40.0 35.0 41.0 35.0 41.0 29 37.89236489510907 40.0 35.0 41.0 35.0 41.0 30 37.83862970367939 40.0 35.0 41.0 35.0 41.0 31 37.70780281101728 40.0 35.0 41.0 35.0 41.0 32 37.603765330786906 40.0 35.0 41.0 34.0 41.0 33 37.478969890483725 40.0 35.0 41.0 34.0 41.0 34 37.30863417982155 40.0 35.0 41.0 34.0 41.0 35 37.177245680522816 40.0 35.0 41.0 34.0 41.0 36 37.09474859001522 40.0 35.0 41.0 33.0 41.0 37 37.03380442242845 40.0 35.0 41.0 33.0 41.0 38 36.906579928978545 39.0 35.0 41.0 33.0 41.0 39 36.890991017874725 39.0 35.0 41.0 33.0 41.0 40 36.809715018949 39.0 35.0 41.0 33.0 41.0 41 36.73709647578407 39.0 35.0 41.0 33.0 41.0 42 36.749942406970845 39.0 35.0 41.0 33.0 41.0 43 36.7078899465847 39.0 35.0 41.0 33.0 41.0 44 36.60089821252723 39.0 35.0 41.0 33.0 41.0 45 36.52080093103757 39.0 35.0 41.0 33.0 41.0 46 36.46025961624541 38.0 35.0 41.0 33.0 41.0 47 36.393845006117395 38.0 35.0 41.0 32.0 41.0 48 36.3097388917072 38.0 35.0 41.0 32.0 41.0 49 36.27255289307988 38.0 35.0 41.0 32.0 41.0 50 36.15644653994211 38.0 35.0 40.0 32.0 41.0 51 36.1041723613142 38.0 35.0 40.0 32.0 41.0 52 35.915458804571635 37.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 0.0 9 2.0 10 1.0 11 1.0 12 4.0 13 2.0 14 7.0 15 23.0 16 44.0 17 126.0 18 244.0 19 504.0 20 848.0 21 1415.0 22 2326.0 23 3963.0 24 6294.0 25 9801.0 26 13268.0 27 15359.0 28 15453.0 29 15677.0 30 16661.0 31 19552.0 32 24376.0 33 33703.0 34 85746.0 35 322738.0 36 72500.0 37 90280.0 38 178316.0 39 743419.0 40 2896.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.326847900689327 24.30766017128704 28.986720778251918 15.378771149771717 2 29.894661454447792 26.444152666288684 30.522455313180746 13.13873056608278 3 28.8876488317269 25.960132493808004 32.30300498343827 12.849213691026826 4 26.31541881770165 27.735609202948286 31.320044164602667 14.628927814747398 5 24.178627913222524 33.02449941810152 28.48849631464295 14.308376354033003 6 22.297156157679566 42.79114320670824 24.239324400942973 10.672376234669214 7 87.4248455730954 4.345140401659156 6.402912476500254 1.8271015487451883 8 88.03861418638655 3.511861776730029 6.390498642236878 2.059025394646534 9 83.00080570558922 5.612962907701949 8.2268508847841 3.1593805019247414 10 44.32586314941362 30.549312166154397 13.742353257139447 11.382471427292531 11 41.779356032347586 21.552326101876996 22.49452418608815 14.173793679687266 12 38.53880815254693 21.189102085882247 25.160514457939183 15.111575303631644 13 20.779027781922355 36.03885291396855 26.646175882546032 16.53594342156307 14 15.568141804183702 38.736653636119485 28.81077855032676 16.884426009370056 15 14.959326788218794 24.707946644385427 44.76368953477963 15.569037032616157 16 17.34290233057802 21.334367819521947 42.735937453373516 18.586792396526512 17 17.01829250096983 21.052072453821133 29.96801050401361 31.961624541195427 18 19.914953298916775 24.504192653158665 36.247441138730565 19.333412909193996 19 27.497836531288232 24.47721643639402 28.177076183939604 19.847870848378143 20 28.484497627644657 23.894482408761302 28.944764405717528 18.676255557876516 21 22.007042463668647 28.7681059950464 30.240159947479935 18.98469159380502 22 21.21404911819999 24.15988779803647 27.661066515472534 26.96499656829101 23 17.72164363940199 30.248873504222495 28.134821401927724 23.894661454447792 24 17.372743278326517 24.15845543254454 40.5830921190057 17.885709170123242 25 16.970427620781237 26.74130882396825 36.04959565515801 20.23866790009251 26 16.41252126167527 34.181015189042405 28.2566918325326 21.149771716749726 27 17.20897615708275 35.47008444988213 28.348184178329504 18.972755214705618 28 15.52421592909791 29.98388588821581 36.104502999015246 18.387395183671035 29 16.652263435886724 26.059204440333023 35.04592521858495 22.24260690519531 30 18.40321088597774 32.298528841275996 30.47841007430396 18.819850198442303 31 27.10023574348721 28.905195309003012 25.814628032586317 18.17994091492346 32 28.284682641520693 25.941153651039954 28.433887380263194 17.340276327176152 33 26.87404135955358 28.459789322908897 25.489063292650176 19.17710602488735 34 19.41756438184477 27.28405598161798 28.502223150607264 24.796156485929995 35 20.837157948136433 25.74909731133061 31.801020560413 21.61272418011996 36 28.62588403807705 27.56551580078183 26.102294768881862 17.70630539225926 37 18.978783086150816 34.770194861388795 29.168869923308765 17.082152129151623 38 20.688669392139893 33.86786428336964 25.80782429649966 19.63564202799081 39 21.235534600578916 31.92283130912238 28.304138939452717 18.537495150845988 40 24.726746441466982 26.12521261675271 28.45871504878995 20.68932589299036 41 17.42347288949897 26.259138790247977 29.927725224553132 26.389663095699916 42 19.694070603682373 26.324072692548718 27.41523678792038 26.566619915848527 43 20.96141565456119 24.72471725702008 29.575064904061353 24.738802184357375 44 18.004655187848766 29.39023007370714 30.449404673092417 22.155710065351673 45 17.596729432126764 36.69362298946615 25.13694010921787 20.572707469189222 46 20.72913371728686 33.11855808540479 26.69821849541941 19.454089701888932 47 19.884694577899793 28.57294619677121 28.211393273850376 23.330965951478618 48 20.45883441258094 27.060427919190715 33.17101847154665 19.309719196681687 49 21.23816060398078 25.897943958700125 33.22413535853899 19.639760078780103 50 19.785145176210797 32.57413983468115 29.01011608128674 18.630598907821312 51 18.641878786070247 32.33702366387157 27.0666348363224 21.95446271373579 52 19.77058279370953 26.910745725284237 34.08277878905434 19.235892691951896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5505.0 1 6117.5 2 6730.0 3 11333.5 4 15937.0 5 10976.5 6 6016.0 7 5986.5 8 5957.0 9 6163.0 10 6369.0 11 6825.0 12 7281.0 13 7149.5 14 6788.5 15 6559.0 16 6282.5 17 6006.0 18 6218.0 19 6430.0 20 6081.0 21 5732.0 22 7291.0 23 8850.0 24 10782.5 25 12715.0 26 13461.0 27 14207.0 28 17573.0 29 20939.0 30 28094.5 31 35250.0 32 37450.0 33 39650.0 34 45488.5 35 51327.0 36 54928.0 37 58529.0 38 66443.0 39 90095.5 40 105834.0 41 129057.5 42 152281.0 43 160412.0 44 168543.0 45 160797.5 46 153052.0 47 144650.0 48 136248.0 49 129980.5 50 123713.0 51 114463.0 52 105213.0 53 94052.0 54 82891.0 55 70435.0 56 57979.0 57 53431.0 58 48883.0 59 44812.5 60 40742.0 61 35180.5 62 29619.0 63 25124.5 64 17580.0 65 14530.0 66 12615.5 67 10701.0 68 9285.0 69 7869.0 70 6646.5 71 5424.0 72 4584.0 73 3744.0 74 3174.5 75 2605.0 76 2205.0 77 1805.0 78 1419.5 79 1034.0 80 757.0 81 480.0 82 348.0 83 216.0 84 156.5 85 97.0 86 68.0 87 39.0 88 24.5 89 6.0 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 1675550.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.221424990157875 #Duplication Level Percentage of deduplicated Percentage of total 1 76.45905917838417 12.402748932802009 2 9.217824222091775 2.990524883822442 3 3.061851773066164 1.4900279660332398 4 1.5238554663695985 0.9887642857422595 5 0.9085013887369401 0.7368593565425267 6 0.6419306722611038 0.6247818149379069 7 0.47441486767436986 0.5386979633136825 8 0.38496639503769003 0.49957628006682997 9 0.3045949108785376 0.4446867149280018 >10 4.3670571998959735 17.297407545727086 >50 1.1333952922163786 12.872403487942389 >100 1.5095891970623296 46.60889337908512 >500 0.009256740232170277 0.9706181920180609 >1k 0.0033324264835812994 1.0158456362377144 >5k 3.702696092868111E-4 0.5181635608007309 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8627 0.5148757124526275 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4649 0.27746113216555757 No Hit CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCTT 2450 0.14622064396765241 Illumina Single End Adapter 1 (95% over 22bp) CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGCT 2185 0.13040494166094715 Illumina Single End Adapter 1 (95% over 21bp) AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA 2000 0.11936379099400196 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.15356151711378355 0.0 2 0.0 0.0 0.0 0.41610217540509087 0.0 3 0.0 0.0 0.0 0.5576079496284801 0.0 4 0.0 0.0 0.0 0.727343260421951 0.0 5 0.0 0.0 0.0 1.2048581062934558 0.0 6 0.0 0.0 0.0 1.5794216824326341 0.0 7 0.0 0.0 0.0 1.813315030885381 0.0 8 0.0 0.0 0.0 2.2749544925546834 0.0 9 0.0 0.0 0.0 2.4460028050490883 0.0 10 0.0 0.0 0.0 2.794186983378592 0.0 11 0.0 0.0 0.0 3.258094357076781 0.0 12 0.0 0.0 0.0 3.6563516457282685 0.0 13 0.0 0.0 0.0 3.817671809256662 0.0 14 0.0 0.0 0.0 3.8713855152039627 0.0 15 0.0 0.0 0.0 3.9592372653755485 0.0 16 0.0 0.0 0.0 4.145623825012683 0.0 17 5.968189549700099E-5 0.0 0.0 4.365253200441646 0.0 18 5.968189549700099E-5 0.0 0.0 4.663782041717645 0.0 19 2.3872758198800395E-4 0.0 0.0 4.812807734773656 0.0 20 2.3872758198800395E-4 0.0 0.0 4.975261854316493 0.0 21 2.3872758198800395E-4 0.0 0.0 5.19184745307511 0.0 22 2.984094774850049E-4 0.0 0.0 5.419951657664647 0.0 23 5.371370594730089E-4 0.0 0.0 5.673301304049416 0.0 24 6.565008504670108E-4 0.0 0.0 5.868222374742622 0.0 25 6.565008504670108E-4 0.0 0.0 6.035152636447734 0.0 26 6.565008504670108E-4 0.0 0.0 6.1902658828444395 0.0 27 6.565008504670108E-4 0.0 0.0 6.345021037868163 0.0 28 6.565008504670108E-4 0.0 0.0 6.500969830801826 0.0 29 6.565008504670108E-4 0.0 0.0 6.676852376831488 0.0 30 6.565008504670108E-4 0.0 0.0 6.879472412043807 0.0 31 6.565008504670108E-4 0.0 0.0 7.07343857240906 0.0 32 6.565008504670108E-4 0.0 0.0 7.257795947599296 0.0 33 6.565008504670108E-4 0.0 0.0 7.445435827041867 0.0 34 6.565008504670108E-4 0.0 0.0 7.63403061681239 0.0 35 7.161827459640118E-4 0.0 0.0 7.864462415326311 0.0 36 7.161827459640118E-4 0.0 0.0 8.052102294768883 0.0 37 7.161827459640118E-4 0.0 0.0 8.246008773238637 0.0 38 7.161827459640118E-4 0.0 0.0 8.431380740652322 0.0 39 7.161827459640118E-4 0.0 0.0 8.638238190444929 0.0 40 7.758646414610128E-4 0.0 0.0 8.86449225627406 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTCG 20 6.3122855E-4 46.0 31 TCGCAAT 20 6.3122855E-4 46.0 40 GTCGAAC 25 3.41783E-5 46.0 28 ACGCATT 20 6.3122855E-4 46.0 43 ACGTTAG 30 1.8619176E-6 46.0 1 GACGTAA 30 1.8619176E-6 46.0 34 GGCCGTA 20 6.3122855E-4 46.0 8 ATAGCCG 25 3.41783E-5 46.0 44 AAGCGTA 40 5.6134013E-9 46.0 41 TACGCTA 40 5.6134013E-9 46.0 13 TACGCCC 90 0.0 46.0 38 GTCGTTA 30 1.8619176E-6 46.0 8 TGTTACG 50 1.6370905E-11 46.0 1 GTCCGAT 30 1.8619176E-6 46.0 21 ACTAACG 30 1.8619176E-6 46.0 20 ACGTCGT 20 6.3122855E-4 46.0 32 CGTAATG 25 3.41783E-5 46.0 21 CGCGTAG 20 6.3122855E-4 46.0 46 CGATCTA 20 6.3122855E-4 46.0 10 TCGACTA 25 3.41783E-5 46.0 45 >>END_MODULE